公开(公告)号：WO0073412A3

公开(公告)日：2002-03-21

申请号：PCT/US0014738

申请日：2000-05-30

Applicant: CEPHEID

Inventor： PETERSEN KURT E ,  TAYLOR MICHAEL T ,  POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  BELGRADER PHILLIP ,  NORTHRUP M ALLEN ,  DORITY DOUGLAS B

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

Abstract translation: 用于从流体样品分离所需分析物的筒在样品流动路径中具有样品流动路径和裂解室（86）。 裂解室包含至少一个用于在样品流过裂解室时从样品捕获细胞或病毒的过滤器。 珠也设置在裂解室中，用于破裂细胞或病毒以从其中释放分析物。 分析物流动路径从裂解室延伸并从样品流动路径发散。 分析物流动路径优选地导致用于化学反应和光学检测分析物的反应室。 该盒还包括至少一个流量控制器（例如阀），用于在样品流过裂解室之后将样品引入废物室，并将从样品分离的分析物引导到分析物流动路径中。

公开(公告)号：WO0073413A2

公开(公告)日：2000-12-07

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：WO0073413A9

公开(公告)日：2002-01-24

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。