公开(公告)号：WO1998024881A1

公开(公告)日：1998-06-11

申请号：PCT/US1997022165

申请日：1997-11-26

Applicant: MERCK & CO., INC. ,  FONG, Tung, M. ,  HUANG, Ruey-Ruey, C. ,  VAN DER PLOEG, Leonardus

Inventor： MERCK & CO., INC.

IPC: C12N01/00

CPC classification number: G01N33/74 ,  C07K14/5759 ,  G01N33/5005 ,  G01N33/6893 ,  G01N2500/00

Abstract: Mutant ob receptors have been made which a) lack a functional first CK-F3 domain; b) lack a functional second CK-F3 domain or c) lack a functional intracellular domain. These receptors may be used in various assays, such as a transactivation assay to identify novel ligands.

Abstract translation: 已经制备了突变体ob受体，其a）缺少功能性第一个CK-F3结构域; b）缺乏功能性第二CK-F3结构域或c）缺乏功能性细胞内结构域。 这些受体可用于各种测定，例如反式激活测定以鉴定新配体。

公开(公告)号：WO1998008932A1

公开(公告)日：1998-03-05

申请号：PCT/US1997007657

申请日：1997-05-05

Applicant: DOW AGROSCIENCES LLC ,  WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： DOW AGROSCIENCES LLC ,  WISCONSIN ALUMNI RESEARCH FOUNDATION ,  ENSIGN, Jerald, C. ,  BOWEN, David, J. ,  PETELL, James ,  FATIG, Raymond ,  SCHOONOVER, Sue ,  FFRENCH-CONSTANT, Richard, H. ,  ROCHELEAU, Thomas, A. ,  BLACKBURN, Michael, B. ,  HEY, Timothy, D. ,  MERLO, Donald, J. ,  ORR, Gregory, L. ,  ROBERTS, Jean, L. ,  STRICKLAND, James, A. ,  GUO, Lining ,  CICHE, Todd, A. ,  SUKHAPINDA, Kitisri

IPC: C12N01/00

CPC classification number: C07K14/195 ,  A01N63/02

Abstract: Proteins from the genus Photorhabdus are toxic to insects upon exposure. Photorhabdus luminescens (formerly Xenorhabdus luminescens) have been found in mammalian clinical samples and as a bacterial symbiont of entomopathogenic nematodes of genus Heterorhabditis. These protein toxins can be applied to, or genetically engineered into, insect larvae food and plants for insect control.

Abstract translation: 来自红豆属的蛋白质在接触时对昆虫有毒。 Photorhabdus luminescens（以前的Xenorhabdus luminescens）已经在哺乳动物临床样品中发现，并且作为Heterorhabditis属的昆虫病原性线虫的细菌共生体。 这些蛋白质毒素可以应用于或遗传工程化成昆虫幼虫食物和植物用于昆虫控制。

公开(公告)号：WO1998004677A1

公开(公告)日：1998-02-05

申请号：PCT/US1997013487

申请日：1997-07-31

Applicant: APHIOS CORPORATION ,  CASTOR, Trevor, P. ,  CHIKARMANE, Hemant, M. ,  HONG, Glenn, T. ,  SHALICE, Christopher

Inventor： APHIOS CORPORATION

IPC: C12N01/00

CPC classification number: C12N1/005 ,  Y10S435/814 ,  Y10S435/816

Abstract: Supercritical and near critical fluids are used to fractionate biomas materials in two steps. In the first step, the biomass is exposed to elevated pressure supercritical or near critical fluid to bring about disruption of the biomass. In the second step, the disrupted biomass is subjected to a multiplicity of supercritical or near critical fluid extraction steps, with different solvation conditions used for each fraction. Thus, fractionation of the biomass is effected.

Abstract translation: 超临界和近临界流体用于在两个步骤中分解生物材料材料。 在第一步中，生物质暴露于超临界或近临界流体的高压下，导致生物量的破坏。 在第二步中，破碎的生物质经受多个超临界或近临界流体萃取步骤，每个馏分使用不同的溶剂化条件。 因此，实现了生物质的分馏。

公开(公告)号：WO1997038088A1

公开(公告)日：1997-10-16

申请号：PCT/US1997004017

申请日：1997-03-14

Applicant: INFECTECH, INC.

Inventor： INFECTECH, INC. ,  OLLAR, Robert-A.

IPC: C12N01/00

CPC classification number: C12N1/28 ,  C12M27/02 ,  C12M41/02 ,  C12M41/26 ,  C12P1/00 ,  C12R1/00

Abstract: A method of growing a paraffinophilic organism including providing a tank and introducing into the tank (i) sterile broth and (ii) a paraffin. The broth is then inoculated with a paraffinophilic organism that will grow and multiply in the broth by utilizing paraffin as its sole carbon source. An associated apparatus is also disclosed.

Abstract translation: 一种生长石蜡生物的方法，包括提供罐并引入罐（i）无菌肉汤和（ii）石蜡。 然后将肉汤接种在石蜡生物体上，通过利用石蜡作为其唯一的碳源，将在肉汤中生长和繁殖。 还公开了一种相关联的装置。

公开(公告)号：WO1997023600A1

公开(公告)日：1997-07-03

申请号：PCT/US1996019747

申请日：1996-12-19

Applicant: UNITED STATES ARMY MEDICAL RESEARCH MATERIEL COMMAND (USAMRMC) ,  CASTRIC, Peter ,  CROSS, Alan ,  SADOFF, Jerald

Inventor： UNITED STATES ARMY MEDICAL RESEARCH MATERIEL COMMAND (USAMRMC)

IPC: C12N01/00

CPC classification number: C07K14/21 ,  A61K39/00 ,  A61K2039/51 ,  Y02A50/472

Abstract: The present invention relates to a broadly reactive vaccine against Gram-negative bacteria which is composed of a biological glycan-pilus sonjugate. The conjugate core is a common pilus type to which is attached the glycan of choice in vivo. Pooling of these bioconjugates produces a multivalent vaccine. These pili give high bronchial titers when delivered by the intranasal route. Mice vaccinated with pure glycosylated P. aeruginosa strain 1244 pili in this manner are protected against respiratory challenge with P. aeruginosa strain 1244. The present invention further relates to a DNA and amino acid sequence of a new gene, pilO, which is capable of glycosylating pilin of Gram-negative bacteria and uses thereof.

Abstract translation: 本发明涉及一种广泛反应性的抗革兰氏阴性细菌疫苗，其由生物聚糖 - 真菌聚合物组成。 共轭核心是常见的菌毛类型，其在体内附着选择的聚糖。 汇集这些生物接头产生多价疫苗。 当鼻内途径递送时，这些皮毛给予高支气管滴度。 以这种方式用纯糖基化的铜绿假单胞菌菌株1244菌毛接种的小鼠被保护免受铜绿假单胞菌菌株1244的呼吸攻击。本发明还涉及能够糖基化的新基因pilO的DNA和氨基酸序列 革兰氏阴性细菌的皮林及其用途。

公开(公告)号：WO1995029984A1

公开(公告)日：1995-11-09

申请号：PCT/US1995005271

申请日：1995-04-27

Applicant: BIOLOG, INC.

Inventor： BIOLOG, INC. ,  BOCHNER, Barry

IPC: C12N01/00

CPC classification number: C12Q1/04

Abstract: The present invention is directed to methods and media for the isolation and presumptive identification of various bacteria. In particular, the organisms commonly associated with urinary tract infections are distinguished based on their colonial morphology and color.

Abstract translation: 本发明涉及用于分离和推测各种细菌的方法和介质。 特别地，根据其殖民地形态和颜色来区分通常与尿路感染相关的生物。

公开(公告)号：WO1994013781A1

公开(公告)日：1994-06-23

申请号：PCT/CA1992000530

申请日：1992-12-04

Applicant: 958075 ONTARIO INC. carrying on business AS ... ,  GASHINSKI, Vladimir Vladislavovich ,  KRINUKOVA, Tatiana Ivanova ,  KOKOSHA, Natalia Vladimirovna ,  ULBERG, Zoya Rudolfovna ,  SOKOLYUK, Anatoliy, Mihailovich

Inventor： 958075 ONTARIO INC. carrying on business AS ...

IPC: C12N01/00

CPC classification number: C12N1/00 ,  C12N1/14 ,  C12N1/20

Abstract: This solid nutrient medium for cultivating microorganisms contains a copolymer of acrylonitrile and acrylamide and a liquid nutrient substrate in the following percent-by-weight proportions: copolymer of acrylonitrile and acrylamide: 5.0 - 30.0; nutrient substrate: 70.0 - 95.0. The method of obtaining a solid culture medium includes the purification of acrylamide and N,N'-methylene-bis-acrylamide by recrystalization; preparation of the reactive mix composed of acrylonitrile, acrylamide, N,N'-methylene-bis-acrylamide, an acceptable solvent and an initiating system; polymerization over a period sufficient for the production of a copolymer retaining the form; its rinsing and saturation with water until equilibrium state; permeation with the nutrient substrate, where the components have these percent-by-weight proportions: acrylonitrile 5.0 - 50.0; acrylamide 5.0 - 50.0; N,N'-methylene-bis-acrylamide 0.01 - 0.60; initiating system 0.15 - 0.3; acceptable solvent residuum.

Abstract translation: 用于培养微生物的固体营养培养基含有以下百分比重量比的丙烯腈和丙烯酰胺的共聚物和液体营养基质：丙烯腈和丙烯酰胺的共聚物：5.0-30.0; 营养底物：70.0〜95.0。 获得固体培养基的方法包括通过重结晶纯化丙烯酰胺和N，N'-亚甲基双丙烯酰胺; 由丙烯腈，丙烯酰胺，N，N'-亚甲基双丙烯酰胺，可接受的溶剂和引发体系组成的反应混合物的制备; 在足以制备保持该形式的共聚物的时间内进行聚合; 用水冲洗并饱和直到平衡状态; 与营养基质渗透，其中组分具有这些百分比重量比：丙烯腈5.0-50.0; 丙烯酰胺5.0〜50.0; N，N'-亚甲基双丙烯酰胺0.01-0.60; 起始系统0.15 - 0.3; 可接受的溶剂残渣。

公开(公告)号：WO1992019716A1

公开(公告)日：1992-11-12

申请号：PCT/DK1992000060

申请日：1992-02-27

Applicant: AGRO-FERM A/S ,  KIEL, Pauli ,  ANDERSEN, Margrethe

Inventor： AGRO-FERM A/S

IPC: C12N01/00

CPC classification number: C12P13/04 ,  C12N1/20 ,  C12P7/40

Abstract: The procedure involves heat treatment of the plant juice, first in a temperature x time interval from 55 DEG C x 24 hours to 120 DEG C x 10 min., whereafter the plant juice is cooled to 50 DEG C - 60 DEG C and the pH value is set at 7.5 - 8.5 by addition of a base. Hereafter the plant juice is converted by enzymic hydrolysis by means of proteolytic enzymes such as proteases and peptidases, while keeping the pH value constant at 7.5 - 8.5 by continuous addition of base until the hydrolysis is over. By the procedure a medium is formed which is well suited as a nutritive substrate for vitamin and amino acid demanding micro-organisms, which form organic acids or amino acids. By the procedure it is possible in a cheap and simple manner to convert plant juice to organic acids or amino acids.

Abstract translation: 该方法涉及植物汁的热处理，首先在55℃×24小时至120℃×10分钟的温度x时间间隔内，将植物汁冷却至50℃〜60℃， 通过添加基数将值设置为7.5-8.5。 此后，通过蛋白水解酶如蛋白酶和肽酶通过酶水解转化植物汁，同时通过连续加入碱保持pH值恒定在7.5-8.5，直到水解结束。 通过该程序，形成适合作为维生素和氨基酸要求微生物的营养底物的培养基，其形成有机酸或氨基酸。 通过该程序，可以以便宜和简单的方式将植物汁转化为有机酸或氨基酸。

公开(公告)号：WO1988003558A1

公开(公告)日：1988-05-19

申请号：PCT/US1987002971

申请日：1987-11-13

Applicant: GENETICS INSTITUTE, INC.

Inventor： GENETICS INSTITUTE, INC. ,  KAUFMAN, Randal, J. ,  DORNER, Andrew

IPC: C12N01/00

CPC classification number: C12N15/85 ,  C07K14/705 ,  C12N9/6459 ,  C12Y304/21069

Abstract: Vectors, improved host cells and improved methods for producing a heterologous protein by culturing an improved eucaryotic host cell of this invention transformed or transfected with a vector capable of directing the expression of the heterologous protein. An expression vector containing DNA encoding an anti-sense RNA to GRP78 protein-encoding mRNA is used to increase levels of heterologous proteins.

Abstract translation: 载体，改良的宿主细胞和通过培养用能够指导异源蛋白质表达的载体转化或转染的本发明的改良的真核宿主细胞产生异源蛋白质的改进方法。 使用含有编码GRP78蛋白编码mRNA的反义RNA的DNA的表达载体来增加异源蛋白质的水平。

公开(公告)号：WO1987007296A1

公开(公告)日：1987-12-03

申请号：PCT/US1987001162

申请日：1987-05-20

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY ... ,  THE DANA-FARBER CANCER INSTITUTE

Inventor： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY ... ,  THE DANA-FARBER CANCER INSTITUTE ,  GOFF, Stephen, P. ,  HASELTINE, William, A. ,  TANESE, Naoko

IPC: C12N01/00

CPC classification number: C07K14/005 ,  C12N9/1276 ,  C12N15/71 ,  C12N2740/16222 ,  C12Q1/703

Abstract: A plasmid which, when introduced into a suitable host cell and grown under appropriate conditions, effects expression of a gene on the plasmid and production of a polypeptide having HTLV-III reverse transcriptase activity. The plasmid is a double-stranded DNA molecule which includes in a 5' to 3' order the following: a DNA sequence which includes an inducible promoter; a DNA sequence which includes an ATG initiation codon; a portion of the Human T-cell lymphotropic virus III (HTLV-III) pol gene, said portion including a DNA sequence which encodes the polypeptide having HTLV-III reverse transcriptase activity; a DNA sequence which contains a gene associated with a selectable or identifiable phenotypic trait which is manifested when the vector is present in the host cell; and a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell. The invention also provides a method for identifying substances which inhibit HTLV-III reverse transcriptase and a method for treating an HTLV-III virus-related disease.

Abstract translation: 一种质粒，当被引入合适的宿主细胞并在合适的条件下生长时，影响基因在质粒上的表达并产生具有HTLV-III逆转录酶活性的多肽。 质粒是双链DNA分子，其以5'至3'的顺序包括以下：包含诱导型启动子的DNA序列; 包括ATG起始密码子的DNA序列; 人类T细胞淋巴细胞病毒III（HTLV-III）pol基因的一部分，所述部分包括编码具有HTLV-III逆转录酶活性的多肽的DNA序列; 包含与可选择或可鉴定的表型性状相关的基因的DNA序列，其在载体存在于宿主细胞中时表现出来; 以及含有能够在宿主细胞中自主复制的细菌质粒的复制起点的DNA序列。 本发明还提供了用于鉴定抑制HTLV-III逆转录酶的物质的方法和用于治疗HTLV-III病毒相关疾病的方法。