利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

365 条记录 (耗时 0.016 秒)

    TRANSGENIC PLANTS HAVING RESISTANCE TO A FUNGAL DISEASE
    51.
    发明申请
    TRANSGENIC PLANTS HAVING RESISTANCE TO A FUNGAL DISEASE 审中-公开
    具有抵抗真菌感染的转基因植物

    公开(公告)号：WO01061021A2

    公开(公告)日：2001-08-23

    申请号：PCT/IL2001/000148

    申请日：2001-02-16

    IPC分类号： C07K14/415 ,  C12N9/10 ,  C12N15/82

    CPC分类号： C12N9/1096 ,  C07K14/415 ,  C12N15/8282

    摘要： The present invention provides novel nucleic acid sequences, which upon their transformation into plants, provide the later with resistance to plant fungal diseases, in particular to downy mildew disease. The nucleic acids of the invention, one or more thereof, are transformed into a plant cell, from which said plant is generated. Such plants also form part of the invention. The nucleic acid of the invention may also be used for mass production of biologically functional proteins or peptides encoded thereby.

    摘要翻译： 本发明提供了新的核酸序列，其在转化为植物后提供对植物真菌病，特别是霜霉病的抗性。 将本发明的一种或多种的核酸转化成植物细胞，从其中产生所述植物。 这些植物也构成本发明的一部分。 本发明的核酸也可用于批量生产由其编码的生物功能性蛋白质或肽。

    THERMOSTABLE ENZYME HAVING AMINOTRANSFERASE ACTIVITY AND GENE ENCODING THE SAME
    52.
    发明申请
    THERMOSTABLE ENZYME HAVING AMINOTRANSFERASE ACTIVITY AND GENE ENCODING THE SAME 审中-公开
    具有氨基转移酶活性的热稳定酶和编码其的基因

    公开(公告)号：WO00060096A1

    公开(公告)日：2000-10-12

    申请号：PCT/JP1999/001696

    申请日：1999-03-31

    IPC分类号： C07H21/04 ,  C12N9/10 ,  C12N15/54 ,  C07K14/195

    CPC分类号： C12N9/1096

    摘要： An aminotransferase which remains stable at high temperatures and over a wide pH range. Thus, a novel catalyst for an aminotransferase reaction to be carried out under severe conditions is provided.

    摘要翻译： 在高温和宽pH范围内保持稳定的氨基转移酶。 因此，提供了在恶劣条件下进行氨基转移酶反应的新型催化剂。

    FRUIT FLAVOUR RELATED GENES AND USE THEREOF
    53.
    发明申请
    FRUIT FLAVOUR RELATED GENES AND USE THEREOF 审中-公开
    水果相关基因及其用途

    公开(公告)号：WO00032789A1

    公开(公告)日：2000-06-08

    申请号：PCT/NL1999/000737

    申请日：1999-12-02

    IPC分类号： A01H5/00 ,  C07K16/40 ,  C12N9/04 ,  C12N9/10 ,  C12N9/18 ,  C12N9/88 ,  C12N15/11 ,  C12N15/53 ,  C12N15/54 ,  C12N15/55 ,  C12N15/60 ,  C12N15/82 ,  C12P7/62 ,  C12Q1/68 ,  G01N33/50

    CPC分类号： C12N9/0006 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/18 ,  C12N9/88 ,  C12N15/8249

    摘要： DNA sequences encoding enzymes involved in the biosynthetic pathway for aliphatic and/or aromatic ester production in fruit are disclosed. The enzymes have alcohol acyl transferase, alcohol dehydrogenase, pyruvate decarboxylase, thiolase, aminotransferase and esterase activities, respectively. The polypeptides having said activities are disclosed. Expression vectors comprising the DNA sequences may be used for regulating ester formation in fruit. Genetically modified plants and microorganisms may be used for producing esters.

    摘要翻译： 公开了编码参与果实中脂肪族和/或芳族酯生产的生物合成途径的酶的DNA序列。 酶分别具有醇酰基转移酶，醇脱氢酶，丙酮酸脱羧酶，硫解酶，氨基转移酶和酯酶活性。 公开了具有所述活性的多肽。 包含DNA序列的表达载体可用于调节果实中的酯形成。 转基因植物和微生物可用于生产酯。

    METHOD FOR STABILIZING ENZYMES AND ENZYME COMPOSITIONS
    55.
    发明申请
    METHOD FOR STABILIZING ENZYMES AND ENZYME COMPOSITIONS 审中-公开
    用于稳定酶和酶组合物的方法

    公开(公告)号：WO99055850A1

    公开(公告)日：1999-11-04

    申请号：PCT/JP1999/002205

    申请日：1999-04-23

    IPC分类号： C12N9/10 ,  C12N9/96

    CPC分类号： C12Y206/01021 ,  C12N9/1096 ,  C12N9/96 ,  C12Y206/01001 ,  C12Y206/01002

    摘要： A method aiming at accurately and stably effecting medical examinations with the use of aspartate aminotransferase or alanine aminotransferase by stabilizing the aspartate aminotransferase or alanine aminotransferase in media. Valine and proline are added to the media (serum, buffer, etc.) as a component for stabilizing aspartate aminotransferase or alanine aminotransferase contained therein.

    摘要翻译： 通过使培养基中天冬氨酸转氨酶或丙氨酸氨基转移酶稳定，旨在通过使用天冬氨酸氨基转移酶或丙氨酸氨基转移酶来准确和稳定地进行体检。 将缬氨酸和脯氨酸作为用于稳定天冬氨酸转氨酶或其中所含的丙氨酸氨基转移酶的组分添加到培养基（血清，缓冲液等）中。

    BIOLOGICAL SYSTEMS FOR MANUFACTURE OF POLYHYDROXYLALKANOATE POLYMERS CONTAINING 4-HYDROXYACIDS
    56.
    发明申请
    BIOLOGICAL SYSTEMS FOR MANUFACTURE OF POLYHYDROXYLALKANOATE POLYMERS CONTAINING 4-HYDROXYACIDS 审中-公开
    用于生产含有4-羟基酸的聚羟基丙酸酯聚合物的生物系统

    公开(公告)号：WO99014313A2

    公开(公告)日：1999-03-25

    申请号：PCT/US1998/019659

    申请日：1998-09-18

    IPC分类号： C12N15/09 ,  C08G63/06 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  C12P7/52 ,  C12P7/62 ,  C12R1/19 ,  C12N15/70 ,  C12P1/04 ,  C12P7/00

    CPC分类号： C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

    摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtained by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, alpha -ketoglutarate, or succinate as substrate.

    摘要翻译： 编码该基因的转移4-羟钴和被集成到细菌表达也合酶从细菌中分离的基因组聚羟基烷酸酯以产生用于制造含有4HB借助于改进的聚羟基烷酸酯的方法 转基因生物包括细菌和植物。 这些新机制提供了一种从廉价碳源如糖和脂肪酸生产含4HB的PHA的方法，其产率高，稳定。 通过筛选其基因组中编码CoA-4HB转移酶和/或PHA合酶的基因的菌株筛选获得有用的菌株以产生聚合物。 生产聚合物的方法包括使用可以利用廉价基材的复合系统。 描述了可以使用氨基酸，α-酮戊二酸或琥珀酸的降解机理作为底物的系统。

    PROCESS FOR PRODUCING L-GLUTAMIC ACID BY FERMENTATION METHOD
    57.
    发明申请
    PROCESS FOR PRODUCING L-GLUTAMIC ACID BY FERMENTATION METHOD 审中-公开
    通过发酵方法生产L-谷氨酸的方法

    公开(公告)号：WO99007853A1

    公开(公告)日：1999-02-18

    申请号：PCT/JP1998/003535

    申请日：1998-08-07

    IPC分类号： C12N9/06 ,  C12N9/10 ,  C12P13/14 ,  C12N15/52 ,  C12N1/19

    CPC分类号： C12N9/0016 ,  C12N9/1096 ,  C12P13/14

    摘要： L-Glutamic acid is efficiently produced at a low cost by culturing a strain which belongs to the genus Corynebacterium, has an enhanced glutamine-oxoglutarate aminotransferase activity and is capable of producing L-Glutamic acid in a liquid medium to accumulate L-Glutamic acid in the culture medium, and harvesting the acid thus accumulated.

    摘要翻译： 通过培养属于棒杆菌属的菌株，能够以低成本有效地生产L-谷氨酸，具有增强的谷氨酰胺 - 氧戊二酸氨基转移酶活性，并且能够在液体培养基中产生L-谷氨酸以将L-谷氨酸积累在 培养基，并收集酸积累。

    PREPARATION OF D-AMINO ACIDS BY DIRECT FERMENTATIVE MEANS
    58.
    发明申请
    PREPARATION OF D-AMINO ACIDS BY DIRECT FERMENTATIVE MEANS 审中-公开
    通过直接发酵方法制备D-氨基酸

    公开(公告)号：WO1998014602A2

    公开(公告)日：1998-04-09

    申请号：PCT/US1997017133

    申请日：1997-09-25

    申请人： MONSANTO COMPANY

    发明人： MONSANTO COMPANY ,  FOTHERINGHAM, Ian, G. ,  TAYLOR, Paul, P. ,  TON, Jennifer, L.

    IPC分类号： C12P13/04

    CPC分类号： C12N9/90 ,  C12N9/1096 ,  C12N9/78 ,  C12P13/04 ,  C12P13/22

    摘要： The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene and an L-aminodeaminase gene; (b) culturing the cell in a cell culture medium; and (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene, an L-aminodeaminase gene and means for increasing production of phenylpyruvate; (b) culturing the cell in a cell culture medium; and (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.

    摘要翻译： 本发明涉及生产天然和非天然D-氨基酸的材料和方法。 特别地，本发明涉及使用重组宿主细胞生产D-氨基酸的发酵方法。 具体地说，本发明涉及一种在细胞中产生D-氨基酸的方法，包括：（a）向细胞中掺入D-氨基转移酶基因和L-氨基脱氨酶基因; （b）在细胞培养基中培养细胞; 和（c）从细胞培养基中分离D-氨基酸。 本发明还涉及一种在细胞中生产D-苯丙氨酸的方法，其包括：（a）向细胞中掺入D-氨基转移酶基因，L-氨基脱氨酶基因和增加苯丙酮酸生产的方法; （b）在细胞培养基中培养细胞; 和（c）从细胞培养基中分离D-苯丙氨酸。 本发明还涉及用于制备对映异构体纯的D-氨基酸的重组细胞的制备。