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199 条记录 (耗时 0.018 秒)

    REGULATION OF HUMAN TRANSKETOLASE-LIKE ENZYME
    63.
    发明申请
    REGULATION OF HUMAN TRANSKETOLASE-LIKE ENZYME 审中-公开
    人类转铁蛋白酶样酶的调控

    公开(公告)号：WO01092310A2

    公开(公告)日：2001-12-06

    申请号：PCT/EP2001/006125

    申请日：2001-05-30

    IPC分类号： A61K48/00 ,  C12N9/10 ,  C07K14/18

    CPC分类号： C12N9/1022 ,  A61K48/00 ,  C07K2319/00

    摘要： Reagents which regulate human transketolase-like enzyme and reagents which bind to human transketolase-like enzyme gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, cancer, anemia, and end-stage renal disease, including sensory periphery neuropathy associated with uremia.

    摘要翻译： 调节人类转酮醇酶样酶的试剂和与人类转酮酶样酶基因产物结合的试剂可以在预防，改善或纠正功能障碍或疾病中发挥作用，包括但不限于癌症，贫血和终末期肾脏 疾病，包括与尿毒症相关的感觉性周围神经病变。

    MATERIALS AND METHODS FOR DETECTION OF OXALOBACTER
    64.
    发明申请
    MATERIALS AND METHODS FOR DETECTION OF OXALOBACTER 审中-公开
    用于检测氧化剂的材料和方法

    公开(公告)号：WO9816632B1

    公开(公告)日：2001-04-12

    申请号：PCT/US9717032

    申请日：1997-09-23

    申请人： UNIV FLORIDA

    发明人： PECK AMMON B ,  SIDHU HARMEET

    IPC分类号： C12N15/09 ,  C12N9/10 ,  C12N9/88 ,  C12Q1/04 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/68 ,  C12R1/01

    CPC分类号： C12N9/10 ,  C12N9/1014 ,  C12N9/1022 ,  C12N9/88 ,  C12Q1/04 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/689 ,  G01N2333/9104 ,  G01N2333/988

    摘要： The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns methods for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.

    摘要翻译： 本发明涉及甲酰辅酶A转移酶与草酰辅酶A脱羧酶一起用于生物样品中草酸盐的检测和测量的新用途。 根据本发明的酶系统的使用导致草酸盐转化为二氧化碳和甲酸盐。 因为甲酸盐的生产与样品中存在的草酸盐浓度直接相关，因此所得甲酸盐浓度的测定为检测甚至低含量草酸盐提供了准确，灵敏和快速的方法。 本发明还涉及克隆，测序和表达编码甲醛 - 辅酶A转移酶和草酸 - 辅酶A脱羧酶酶的生物素。 本发明还涉及用于检测样品中生物形态产杆菌生物体的存在的方法，以及用于检测方法中的多核苷酸探针和引物。

    NOVEL TRANSALDOLASE GENE
    65.
    发明申请
    NOVEL TRANSALDOLASE GENE 审中-公开
    新型转移酶基因

    公开(公告)号：WO01021774A1

    公开(公告)日：2001-03-29

    申请号：PCT/JP2000/006471

    申请日：2000-09-21

    IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P19/18

    CPC分类号： C12N9/1022 ,  C12Y202/01002

    摘要： Attempts are made to provide a novel transaldolase gene; a polypeptide encoded by this gene; a recombinant DNA obtained by integrating this gene; a microorganism carrying this recombinant DNA; and a process for producing an aromatic amino acid, an aromatic vitamin, L-histidine, riboflavin, a nucleic acid, a nucleic acid-associated substance, a novel saccharide, etc. by using the above microorganism. As the results of extensive studies, a novel transaldolase gene is isolated from chromosomal DNA of a microorganism belonging to the genus Corynebacterium as a DNA fragment complementary to the requirement for shikimic acid of a transketolase defective variant obtained as a variant with the requirement for shikimic acid belonging to the genus Corynebacterium . Further, a recombinant DNA containing this gene is constructed and transferred into a host microorganism, thereby achieving the objects as described above.

    摘要翻译： 尝试提供新的转醛醇酶基因; 由该基因编码的多肽; 通过整合该基因获得的重组DNA; 携带该重组DNA的微生物; 以及通过使用上述微生物生产芳香族氨基酸，芳香族维生素，L-组氨酸，核黄素，核酸，核酸相关物质，新型糖等的方法。 作为广泛研究的结果，从属于棒杆菌属微生物的微生物的染色体DNA中分离出新的转醛醇酶基因，作为与获得的转酮醇酶缺陷型变体莽草酸的要求互补的DNA片段 具有对属于棒状杆菌属的莽草酸的要求的变体。 此外，构建含有该基因的重组DNA并转移到宿主微生物中，从而实现如上所述的目的。

    DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE POLYPEPTIDE
    66.
    发明申请
    DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE POLYPEPTIDE 审中-公开
    脱氧木糖-5-磷酸酯合酶多肽

    公开(公告)号：WO01014409A1

    公开(公告)日：2001-03-01

    申请号：PCT/US2000/022332

    申请日：2000-08-16

    IPC分类号： C07K14/21 ,  C12N9/10 ,  C07K1/00

    CPC分类号： C12N9/1022 ,  C07K14/21

    摘要： The invention provides dxs polypeptides and polynucleotides encoding dxs polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing dxs polypeptides to screen for antibacterial compounds.

    摘要翻译： 本发明提供了dxs多肽和编码dxs多肽的多核苷酸以及通过重组技术产生这种多肽的方法。 还提供了利用dxs多肽筛选抗菌化合物的方法。

    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE OPCA GENE
    67.
    发明申请
    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE OPCA GENE 审中-公开
    用于OPCA基因的核苷酸序列

    公开(公告)号：WO2001004322A1

    公开(公告)日：2001-01-18

    申请号：PCT/EP2000/006300

    申请日：2000-07-05

    申请人： DEGUSSA AG ,  FORSCHUNGSZENTRUM JÜLICH GMBH ,  NATIONAL UNIVERSITY OF IRELAND

    发明人： MCCORMACK, Ashling ,  STAPELTON, Cliona ,  BURKE, Kevin ,  MORITZ, Bernd ,  EGGELING, Lothar ,  SAHM, Hermann ,  MÖCKEL, Bettina ,  WEISSENBORN, Anke

    IPC分类号： C12N15/53

    CPC分类号： C12N9/0006 ,  C07K2319/00 ,  C12N9/1022 ,  C12P13/06 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide from coryneform bacteria, comprising at least one polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for polypeptides which comprise at least one of the amino acid sequences according to SEQ ID No. 3 or SEQ ID No. 5 or SEQ ID No. 8 or SEQ ID No. 10, b) polynucleotide which codes for polypeptides which comprise amino acid sequences which are identical to the extent of at least 70 % to the amino acid sequences according to SEQ ID No. 3 or SEQ ID No. 5 or according to SEQ ID No. 8 or SEQ ID No. 10, c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and a process for the fermentative preparation of L-amino acids, which comprises in the coryneform microorganisms which in particular already produce L-amino acids, a) amplifying, in particular over-expressing, in addition to the opcA gene, at least one of the nucleotide sequences which code for the tal gene, tkt gene, zwf gene or the devB gene, b) concentrating the desired L-amino acid in the medium or in the cells of the bacteria and c) isolating the L-amino acid.

    摘要翻译： 本发明涉及来自棒状细菌的分离的多核苷酸，其包含至少一种选自以下的多核苷酸序列：a）与编码多肽的多核苷酸程度相同的多核苷酸，所述多核苷酸编码多肽，所述多核苷酸包含至少一个 根据SEQ ID No.3或SEQ ID No.5或SEQ ID No.8或SEQ ID No.10的氨基酸序列，b）编码包含氨基酸序列的多肽的多核苷酸，所述氨基酸序列与 至少70％与根据SEQ ID No.3或SEQ ID No.5或SEQ ID No.8或SEQ ID No.10的氨基酸序列，c）与a）或b的多核苷酸互补的多核苷酸 ）或d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及L-氨基酸的发酵制备方法，其包含在特别是已经发育的棒状微生物中 d）L-氨基酸，a）除了opcA基因外，特别是过表达，至少一个编码tal基因，tkt基因，zwf基因或devB基因的核苷酸序列之一，b）浓缩 培养基中或细胞中所需的L-氨基酸，和c）分离L-氨基酸。

    METHODS OF MODIFYING THE PRODUCTION OF ISOPENTENYL PYROPHOSPHATE, DIMETHYLALLYL PYROPHOSPHATE AND/OR ISOPRENOIDS
    68.
    发明申请
    METHODS OF MODIFYING THE PRODUCTION OF ISOPENTENYL PYROPHOSPHATE, DIMETHYLALLYL PYROPHOSPHATE AND/OR ISOPRENOIDS 审中-公开
    磷酸异戊酯，二甲基戊二酸和/或异丙苯磺酸的生产方法

    公开(公告)号：WO99058649A1

    公开(公告)日：1999-11-18

    申请号：PCT/US1999/007041

    申请日：1999-05-13

    IPC分类号： C12N9/02 ,  C12N9/10 ,  C07H21/04 ,  C12N1/20 ,  C12N9/04 ,  C12N15/00

    CPC分类号： C12N9/0008 ,  C12N9/1022

    摘要： The present invention provides methods for enhancing and reducing the levels of IPP, DMAPP and/or isoprenoids in a host cell. Nucleic acid sequences encoding DXP synthase, GAP dehydrogenase, and LYTB as well as vectors containing the same and host cells transformed with the vectors.

    摘要翻译： 本发明提供用于增强和降低宿主细胞中IPP，DMAPP和/或类异戊二烯的水平的方法。 编码DXP合酶，GAP脱氢酶和LYTB的核酸序列以及含有与载体转化的相同载体和宿主细胞的载体。

    SYNTHESIS OF ADIPIC ACID FROM BIOMASS-DERIVED CARBON SOURCES
    70.
    发明申请
    SYNTHESIS OF ADIPIC ACID FROM BIOMASS-DERIVED CARBON SOURCES 审中-公开
    从生物质衍生的碳源合成ADIPIC

    公开(公告)号：WO1995007996A1

    公开(公告)日：1995-03-23

    申请号：PCT/US1994010263

    申请日：1994-09-02

    申请人： PURDUE RESEARCH FOUNDATION

    发明人： PURDUE RESEARCH FOUNDATION ,  FROST, John, W. ,  DRATHS, Karen, M.

    IPC分类号： C12P07/44

    CPC分类号： C12N9/0069 ,  C12N9/1022 ,  C12N9/88 ,  C12N15/52 ,  C12P7/44 ,  C12Y113/11001 ,  Y10S435/849 ,  Y10S435/852

    摘要： A method is provided for producing adipic acid. The method comprises the steps of culturing a cell transformant characterized by the constitutive expression of structural genes encoding 3-dehydroshikimate dehydratase, protocatechuate decarboxylase, and catechol 1,2-dioxygenase for a period of time sufficient to convert a carbon source to cis, cis-muconic acid and hydrogenating the cis, cis-muconic acid to produce adipic acid.

    摘要翻译： 提供了一种生产己二酸的方法。 该方法包括培养细胞转化体的步骤，其特征在于将编码3-脱水莽草酸脱水酶，原儿茶酸脱羧酶和邻苯二酚1,2-双加氧酶的结构基因的组成型表达足以将碳源转化成顺式，顺式 - 粘酸和氢化顺式，顺式 - 粘酸产生己二酸。