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    GENETICALLY MODIFIED HOST CELLS AND USE OF SAME FOR PRODUCING ISOPRENOID COMPOUNDS
    2.
    发明申请
    GENETICALLY MODIFIED HOST CELLS AND USE OF SAME FOR PRODUCING ISOPRENOID COMPOUNDS 审中-公开
    遗传修饰的宿主细胞及其用于生产异噻唑类化合物的用途

    公开(公告)号：WO2006014837A1

    公开(公告)日：2006-02-09

    申请号：PCT/US2005/026190

    申请日：2005-07-21

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  KIRBY, James ,  KEASLING, Jay D. ,  PARADISE, Eric M.

    Inventor： KIRBY, James ,  KEASLING, Jay D. ,  PARADISE, Eric M.

    IPC: C12N1/15

    CPC classification number: C12P5/007 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/81 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y205/01092 ,  C12Y402/03024

    Abstract: The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound.

    Abstract translation: 本发明提供了产生类异戊二烯前体或类异戊二烯化合物的遗传修饰的真核宿主细胞。 受试者遗传修饰的宿主细胞包括甲羟戊酸途径酶中的一种或多种的增加的活性水平，异戊烯转移酶活性水平增加和角鲨烯合酶活性水平降低。 提供了用于在受试者遗传修饰的真核宿主细胞中产生类异戊二烯化合物或类异戊二烯前体的方法。 所述方法通常涉及在促进产生高水平的类异戊二烯或类异戊二烯前体化合物的条件下培养受试者遗传修饰的宿主细胞。

    METHOD FOR THE ENZYMATIC PRODUCTION OF 3-BUTEN-2-ONE
    5.
    发明申请
    METHOD FOR THE ENZYMATIC PRODUCTION OF 3-BUTEN-2-ONE 审中-公开
    3- BUTEN-2-ONE的生产方法

    公开(公告)号：WO2015000981A3

    公开(公告)日：2015-02-19

    申请号：PCT/EP2014064102

    申请日：2014-07-02

    Applicant: SCIENTIST OF FORTUNE SA ,  GLOBAL BIOENERGIES

    Inventor： MARLIERE PHILIPPE ,  ANISSIMOVA MARIA ,  ALLARD MATHIEU

    IPC: C12P5/02 ,  C12N1/00 ,  C12P7/16 ,  C12P7/26

    CPC classification number: C12P7/16 ,  C12N1/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/26 ,  C12P19/32 ,  C12Y101/01001 ,  C12Y101/0103 ,  C12Y101/01034 ,  C12Y101/01184 ,  C12Y102/0105 ,  C12Y103/08001 ,  C12Y402/01017 ,  C12Y402/01018 ,  C12Y402/01055 ,  C12Y402/01059 ,  C12Y402/01074 ,  C12Y402/01116 ,  C12Y402/01127 ,  Y02E50/10

    Abstract: Described is a method for the production of 3-buten-2-one comprising the enzymatic conversion of 4-hydroxy-2-butanone into 3-buten-2-one by making use of an enzyme catalyzing 4-hydroxy-2-butanone dehydration, wherein said enzyme catalyzing 4-hydroxy-2-butanone dehydration is (a) a 3-hydroxypropiony-CoA dehydratase (EC 4.2.1.116), (b) a 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55), (c) an enoyl-CoA hydratase (EC 4.2.1.17), (d) a 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.59), (e) a crotonyl-[acyl-carrier-protein] hydratase (EC 4.2.1.58), (f) a 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.60), (g) a 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.61 ), (h) a long-chain-enoyl-CoA hydratase (EC 4.2.1.74), or (i) a 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18). The produced 3-buten-2-one can be further converted into 3-buten-2-ol and finally into 1,3-butadiene.

    Abstract translation: 描述了制备3-丁烯-2-酮的方法，其包括通过使用催化4-羟基-2-丁酮脱水的酶将4-羟基-2-丁酮酶促转化成3-丁烯-2-酮 其中所述催化4-羟基-2-丁酮脱水的酶是（a）3-羟基丙酰辅酶A脱水酶（EC 4.2.1.116），（b）3-羟基丁酰辅酶A脱水酶（EC 4.2.1.55），（c ）烯酰辅酶A水合酶（EC 4.2.1.17），（d）3-羟基辛酰 - [酰基 - 载体 - 蛋白]脱水酶（EC 4.2.1.59），（e）巴豆酰基 - [酰基 - 载体 - 蛋白]水合酶 （EC 4.2.1.58），（f）3-羟基癸酰基 - [酰基 - 载体 - 蛋白质]脱水酶（EC 4.2.1.60），（g）3-羟基棕榈酰 - [酰基 - 载体 - 蛋白质]脱水酶（EC 4.2。 1.61），（h）长链烯酰辅酶A水合酶（EC 4.2.1.74），或（i）3-甲基谷丙酰辅酶A水合酶（EC 4.2.1.18）。 生成的3-丁烯-2-酮可以进一步转化为3-丁烯-2-醇，最后进一步转化为1,3-丁二烯。

    A METHOD FOR REGULATING CYTOSOLIC ISOPRENOID BIOSYNTHESIS IN HEVEA BRASILIENSIS
    6.
    发明申请
    A METHOD FOR REGULATING CYTOSOLIC ISOPRENOID BIOSYNTHESIS IN HEVEA BRASILIENSIS 审中-公开
    一种用于调节血管性支气管炎生物活性的方法

    公开(公告)号：WO2013039378A1

    公开(公告)日：2013-03-21

    申请号：PCT/MY2012/000214

    申请日：2012-07-23

    Applicant: UNIVERSITI SAINS MALAYSIA ,  MAQSUDUL, Alam ,  MOHD NAZALAN, Mohd Najimudin ,  SAITO, Jennifer Ann ,  BISWAPRIYA, Biswavas Misra

    Inventor： MAQSUDUL, Alam ,  MOHD NAZALAN, Mohd Najimudin ,  SAITO, Jennifer Ann ,  BISWAPRIYA, Biswavas Misra

    IPC: C12N9/10 ,  C12N9/02 ,  C12N9/12 ,  C12N9/14 ,  A01H5/00 ,  C12N15/29 ,  C12N15/52

    CPC classification number: C12N15/8243 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/88 ,  C12Y101/01034 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y401/01033

    Abstract: The present invention discloses an isolated polynucleotide encoding an enzyme for catalyzing cytosolic biosynthesis of isopentenyl diphosphate, dimefhylallyl diphosphate or the combination thereof in the plant of Hevea brasiliensis , comprising nucleotide sequence set forth in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ' ID NO. 9 or any complementary sequence thereof; and a method for enhancing cytoplasmic availability and functionality of the enzyme, comprising the steps of predicting catalytic domain, signal peptide or membrane-spanning domain of the enzyme; introducing a point mutation to the predicted catalytic domain, signal peptide or membrane-spanning transmembrane domain; and expressing the mutated catalytic domain, signal peptide or membrane-spanning transmembrane domain in a plant cell, tissue or organ of Hevea brasiliensis . The present invention also relates to an isolated polypeptide encoded by the polynucleotide, a recombinant gene construct comprising the polynucleotide, a transformant and a transgenic plant comprising the recombinant gene construct, with enhanced production of cytosolic isoprenoid towards rubber production.

    Abstract translation: 本发明公开了一种分离的多核苷酸，其编码用于催化异二烯基二磷酸，二烯丙基二磷酸或其组合的细胞质生物合成的酶，其包含在SEQ ID NO。 1，SEQ ID NO： 3，SEQ ID NO： 5，SEQ ID NO： 7，SEQ ID NO： 9或其任何互补序列; 以及用于增强酶的细胞质可用性和功能的方法，包括预测酶的催化结构域，信号肽或跨膜结构域的步骤; 对预测的催化结构域，信号肽或跨膜跨膜结构域引入点突变; 并在巴西三叶草的植物细胞，组织或器官中表达突变的催化结构域，信号肽或跨膜跨膜结构域。 本发明还涉及由多核苷酸编码的分离的多肽，包含多核苷酸的重组基因构建体，转化体和包含重组基因构建体的转基因植物，其具有增强的产生细胞质类异戊二烯用于橡胶生产的功能。

    METHODS AND COMPOSITIONS FOR DETECTING MICROBIAL PRODUCTION OF WATER-IMMISCIBLE COMPOUNDS
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR DETECTING MICROBIAL PRODUCTION OF WATER-IMMISCIBLE COMPOUNDS 审中-公开
    检测水中不溶性化合物微生物的方法和组合物

    公开(公告)号：WO2012158466A1

    公开(公告)日：2012-11-22

    申请号：PCT/US2012/037351

    申请日：2012-05-10

    Applicant: AMYRIS, INC. ,  UBERSAX, Jeff ,  FRENZ, Lucas

    Inventor： UBERSAX, Jeff ,  FRENZ, Lucas

    IPC: G01N33/58 ,  C12P5/00

    CPC classification number: C12Q1/02 ,  C12N9/0006 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y203/01009 ,  C12Y203/0301 ,  G01N33/582

    Abstract: Provided herein are methods and compositions useful for detecting the production of compounds in a cell, for example, a microbial cell genetically modified to produce one or more such compounds at greater yield and/or with increased persistence compared to a parent microbial cell that is not genetically modified. In some embodiments, the methods comprise contacting a solution with a fluorescent dye that directly binds the recombinantly produced compound, wherein the solution comprises a plurality of cells recombinantly producing the compound; and detecting the fluorescent dye under spectral conditions suitable for the selective detection of the fluorescent dye bound to the recombinantly produced compound.

    Abstract translation: 本文提供了用于检测细胞中化合物的生产的方法和组合物，例如，与不是亲本微生物细胞相比，经遗传修饰以产生一种或多种此类化合物以更高的产率和/或具有增加的持久性的微生物细胞 转基因 在一些实施方案中，所述方法包括使溶液与直接结合重组产生的化合物的荧光染料接触，其中所述溶液包含重组产生所述化合物的多个细胞; 并在适于选择性检测与重组产生的化合物结合的荧光染料的光谱条件下检测荧光染料。

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