公开(公告)号：WO00054886A1

公开(公告)日：2000-09-21

申请号：PCT/US2000/005921

申请日：2000-03-07

IPC: G01N33/48 ,  A61M1/02 ,  A61M1/36 ,  B04B5/04 ,  G01N1/10 ,  G01N1/28

CPC classification number: A61M1/3693 ,  A61M1/3696 ,  A61M2202/0427 ,  B04B5/0442 ,  B04B2005/045 ,  B04B2005/0471

Abstract: An apparatus and method are provided for separating components of a fluid or particles. A separation vessel (28) having a barrier dam (62) is provided to initially separate intermediate density components of a fluid, and a fluid chamber (30) is provided to further separate these intermediate density components by forming an elutriative field or saturated fluidized particle bed. The separation vessel includes a shield (96) for limiting flow into the fluid chamber of relatively high density substances, such as red blood cells. The separation vessel also includes a trap dam (70) with a smooth, gradually sloped downstream section (104) for reducing mixing of substances. Structure is also provided for adding additional plasma to platelets and plasma flowing from the fluid chamber. The system reduces clumping of platelets by limiting contact between the platelets and walls of the separation vessel.

Abstract translation: 提供了用于分离流体或颗粒的组分的装置和方法。 提供具有阻挡坝（62）的分离容器（28）以最初分离流体的中间密度分量，并且提供流体室（30）以进一步分离这些中间密度组分，通过形成淘析场或饱和流化颗粒 床。 分离容器包括用于限制流入诸如红细胞等相对高密度物质的流体室的屏蔽件（96）。 分离容器还包括具有平滑，逐渐倾斜的下游部分（104）的捕集坝（70），用于减少物质的混合。 还提供了结构，用于将额外的血浆添加到血小板和从流体腔流出的血浆。 该系统通过限制分离容器的血小板和壁之间的接触来减少血小板的聚集。

公开(公告)号：WO00047328A1

公开(公告)日：2000-08-17

申请号：PCT/GB2000/000401

申请日：2000-02-08

IPC: B04B5/02 ,  B04B5/04 ,  B04B7/02 ,  B04B7/08 ,  B04B15/00

CPC classification number: B04B7/08 ,  B04B5/0421 ,  B04B7/02 ,  B04B2007/025

Abstract: A centrifuge rotor including a sample carrier (1) enclosed within an outer housing (7, 8) and being rotatable about a principal axis of rotation of the rotor, the sample carrier (1) having a peripheral zone for holding sample tubes (6) in an orientation parallel to the said axis when at rest and the sample carrier being capable of deflecting to enable sample tubes (6) to swing out under the effect of centrifugal force. The swinging out of the tubes (6) is constrained and controlled by the arrangement of the sample carrier (1) and contact between the tubes (6) and outer housing (7, 8).

Abstract translation: 一种离心机转子，其包括封闭在外壳体（7,8）内并可围绕转子的主旋转轴线旋转的样品载体（1），样品载体（1）具有用于保持样品管（6）的周边区域， 在静止时平行于所述轴线的取向，并且样品载体能够偏转以使样品管（6）在离心力作用下摆动。 通过样品载体（1）的布置和管（6）和外壳（7,8）之间的接触来限制和控制管（6）摆动。

公开(公告)号：WO00047327A1

公开(公告)日：2000-08-17

申请号：PCT/GB2000/000172

申请日：2000-01-21

IPC: B01D1/22 ,  B04B5/02 ,  B04B5/04 ,  B01D1/14 ,  B01L3/14 ,  B04B7/02 ,  B04B15/08

CPC classification number: B01D1/223 ,  B04B5/02 ,  B04B5/0421

Abstract: A centrifugal evaporator, in which inclined sample tubes (61) supported in a holder (62) are rotatably mounted on a rotor (64), within a vacuum chamber (76), has a rotatable drive shaft (63) connected to the holder. Drive to the shaft (63) is via a spindle (70) passing up through the centre of a main drive shaft (71) and connected by bevel gears (67), (69) to the shaft (63). In operation, the relatively slow rotation of the holder (62) mounted on the shaft (63), in combination with rotation about the main shaft (71), causes the liquid sample in each tube (61) to be swept around the inside walls of the tube to simulate a vortex, thereby increasing the exposed surface area and improving evaporation. In an alternative arrangement the holder is pivoted relative to the rotor, so that the liquid sample is alternatively slushed from one side wall to the other.

Abstract translation: 其中支撑在保持器（62）中的倾斜样品管（61）可旋转地安装在转子（64）上的真空室（76）内的离心式蒸发器具有连接到保持器的可旋转的驱动轴（63）。 通过经过主驱动轴（71）的中心的主轴（70）向轴（63）传递并通过锥齿轮（67），（69）连接到轴（63）。 在操作中，安装在轴（63）上的保持器（62）相对慢的旋转与围绕主轴（71）的旋转相结合，使得每个管（61）中的液体样品围绕内壁 以模拟涡流，从而增加暴露的表面积并改善蒸发。 在替代布置中，保持器相对于转子枢转，使得液体样品被替代地从一个侧壁冲入另一个侧壁。

公开(公告)号：WO00038762A1

公开(公告)日：2000-07-06

申请号：PCT/IB1999/002053

申请日：1999-12-24

IPC: A61J3/00 ,  A61M1/02 ,  A61M1/36 ,  B04B5/04

CPC classification number: A61M1/3693 ,  A61M1/0209 ,  A61M1/3696 ,  A61M1/3698 ,  B04B5/0442 ,  B04B2005/0485

Abstract: A system for separating biological fluids into components, comprises a set of containers (40, 42-44) for the biological fluid to be separated and the separated components, optionally an additional container (41) for additive solution, and a hollow centrifugal processing chamber (20) having an axial inlet/outlet (7) for the biological fluid. The processing chamber contains a piston (21) movable to intake a selected quantity of biological fluid and express processed biological fluid components via the outlet. Optical means (60-69) monitor the position of piston (21) to control the amount of intaken fluid and the expression of components. A distribution valve arrangement (45-48) selectively communicates the processing chamber (20) and the containers (40-44) or places them out of communication. The system is arranged to operate in a separation mode and in a non-separation transfer mode, especially for adding preservative solution to separated blood stem cells. In the separation mode, fluids are intaken into processing chamber (20), centrifuged and separated into components, and the components expressed possibly through the use of a density gradient product. In the transfer mode, the processing chamber (20) intakes and expresses fluid with the chamber stationary, the valve actuation arrangement (45-48) transferring fluid from one container (40-44) to another via the processing chamber (20), by movement of the piston (21), without centrifugation or separation, and the means (60-69) for monitoring the piston's position controls the amounts of non-separated fluids transferred.

Abstract translation: 用于将生物流体分离成组件的系统包括用于待分离的生物流体的一组容器（40,42-44）和分离的组分，任选的用于添加剂溶液的附加容器（41）和中空离心处理室 （20）具有用于生物流体的轴向入口/出口（7）。 处理室包含可移动以吸入选定数量的生物流体并通过出口表达经处理的生物流体成分的活塞（21）。 光学装置（60-69）监视活塞（21）的位置，以控制吸入液体的数量和组分的表达。 分配阀装置（45-48）选择性地连通处理室（20）和容器（40-44），或将它们放在通信中。 该系统被布置成以分离模式和非分离转移模式操作，特别是用于向分离的血液干细胞添加防腐溶液。 在分离模式中，流体进入处理室（20），离心分离成分量，并且组分可能通过使用密度梯度产物表达。 在传送模式中，处理室（20）进入和表达流体，腔室静止，阀致动装置（45-48）通过处理室（20）将流体从一个容器（40-44）传送到另一个容器（40-44） 没有离心或分离的活塞（21）的运动，以及用于监测活塞位置的装置（60-69）控制非分离流体转移量。

公开(公告)号：WO00029121A1

公开(公告)日：2000-05-25

申请号：PCT/GB1999/003746

申请日：1999-11-10

IPC: B04B5/04

CPC classification number: B04B5/0421

Abstract: Centrifuge apparatus comprises a rotor (20) having an axis of rotation (22) and a sample holder (24), the longitudinal axis (28) of the sample holder being oriented to extend along a chord of a circle defined by the rotational path of the perimeter of the rotor, the chord being spaced from the axis of rotation by as great a distance as possible. The sample holder may be fixed relative to rotor, but is preferably movable during operation of the apparatus such that the angle between the centrifugal force direction and the longitudinal axis may be varied in a predetermined manner whilst the apparatus is operating at effective centrifugation speeds, said angle being controlled by a biassing mechanism (32). A similar biassing mechanism may also be applied to an otherwise conventional "swinging-bucket" type centrifuge. The apparatus provides improved speed and uniformity of centrifugation.

Abstract translation: 离心设备包括具有旋转轴线（22）和样本保持器（24）的转子（20），样本保持器的纵向轴线（28）被定向成沿着由 转子的周边，弦与旋转轴线间隔尽可能大的距离。 样品保持器可以相对于转子固定，但是优选在装置的操作期间可移动，使得离心力方向和纵向轴线之间的角度可以以预定的方式变化，同时装置以有效的离心速度运行，所述 角度由偏压机构（32）控制。 类似的偏压机构也可以应用于另外常规的“摆动式”型离心机。 该设备提供了改善的离心速度和均匀性。

公开(公告)号：WO9912590A8

公开(公告)日：1999-09-02

申请号：PCT/US9818254

申请日：1998-09-02

Applicant: COBE LAB

Inventor： DUMONT LARRY JOE ,  TAYLOR LINDA A ,  VANWAEG GEERT

IPC: A61M1/36 ,  B04B5/04

CPC classification number: B04B5/0442 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3633 ,  A61M1/3693 ,  A61M1/3696 ,  B04B2005/0471

Abstract: The instant invention relates to a method and the apparatus for collecting a hyperconcentrated platelet product. A fluid containing platelets and other particles flows into a fluid chamber at a flow rate. The flow rate of the fluid is selected to retain the majority of the platelets in the fluid chamber in a saturated bed. The platelets are collected from the fluid chamber without collecting the other particles to form a hyperconcentrated other particle reduced platelet product.

Abstract translation: 本发明涉及用于收集高浓度血小板产品的方法和设备。 含有血小板和其他颗粒的流体以流速流入流体室。 选择流体的流速以将大部分血小板保留在流体室中的饱和床中。 从流体室收集血小板而不收集其他颗粒以形成高浓缩的其他颗粒减少的血小板产品。

公开(公告)号：WO99036111A1

公开(公告)日：1999-07-22

申请号：PCT/US1999/000353

申请日：1999-01-19

IPC: A61K35/14 ,  A61M1/02 ,  A61M1/36 ,  B04B5/00 ,  B04B5/04

CPC classification number: A61M1/3693 ,  A61M1/0227 ,  A61M1/3696 ,  A61M2205/12 ,  B04B5/0442 ,  B04B2005/0471

Abstract: A system and method are disclosed for separating particles having different sedimentation velocities. The system includes a fluid chamber for separating the particles from one another. Conduits are provided for flowing liquid carrying particles to the fluid chamber and for mixing the liquid with a diluting liquid. Structure is also provided for separating particles from the liquids after the particles are separated in the fluid chamber. The disclosed methods include forming a saturated fluidized bed of first particles to retain second particles in the fluid chamber. A diluting liquid having a density less than that of the particles is mixed with the liquid carrying particles to reduce overall density of substances in the fluid chamber and thereby reduce Coriolis jetting of liquids in the fluid chamber.

Abstract translation: 公开了用于分离具有不同沉降速度的颗粒的系统和方法。 该系统包括用于将颗粒彼此分离的流体室。 提供导管，用于将液体携带颗粒流动到流体室，并将液体与稀释液体混合。 还提供了在流体室中分离颗粒之后从液体中分离颗粒的结构。 所公开的方法包括形成第一颗粒的饱和流化床以将第二颗粒保留在流体室中。 将密度小于颗粒的稀释液与载液颗粒混合以降低流体室中的物质的总体密度，从而减少流体腔中的液体的科里奥利喷射。

公开(公告)号：WO99033538A1

公开(公告)日：1999-07-08

申请号：PCT/GB1998/003661

申请日：1998-12-08

IPC: B01D3/08 ,  B04B5/04 ,  B04B9/02 ,  B04B13/00 ,  B04B15/02 ,  B04B15/08 ,  G01N1/40 ,  G01N35/00 ,  B01D1/22 ,  B04B5/02 ,  B01D3/10 ,  F26B5/04 ,  F26B5/08

CPC classification number: B04B15/02 ,  B01D3/08 ,  B04B5/0421 ,  B04B9/02 ,  B04B13/00 ,  B04B15/08 ,  G01N2001/4027 ,  G01N2035/00495 ,  Y10S159/16

Abstract: In a centrifugal evaporator for evaporating a mixture of liquids having different volatilities and specific gravities, the mixture being contained in a test tube (14) pivotally mounted on a rotatable support arm (12) in a sealable vacuum chamber (16), the support arm is rotated up to a speed of the order of 2000 rpm before evaporation of the more volatile component occurs, and only then is the chamber progressively evacuated. The motor is preferably a three-phase induction motor connected to the electric supply in a frequency convertor.

Abstract translation: 在用于蒸发具有不同挥发性和比重的液体混合物的离心式蒸发器中，该混合物容纳在可密封的真空室（16）中可旋转地安装在可旋转的支撑臂（12）上的试管（14）中，该支撑臂 旋转到2000rpm的速度，然后蒸发更易挥发的组分，然后只有室逐渐抽真空。 电动机优选是在变频器中连接到电源的三相感应电动机。

公开(公告)号：WO9852629A3

公开(公告)日：1999-02-25

申请号：PCT/US9810406

申请日：1998-05-20

Applicant: ZYMEQUEST INC ,  JORGENSEN GLEN ,  BARRY DONALD ,  EDWARDS BRUCE H ,  FENNELLY JEREMY ,  BRIEN JOHN P O ,  SACCO VICTOR JR ,  MARTIN ROY E III ,  SUSSER MARK

Inventor： JORGENSEN GLEN ,  BARRY DONALD ,  EDWARDS BRUCE H ,  FENNELLY JEREMY ,  O'BRIEN JOHN P ,  SACCO VICTOR JR ,  MARTIN ROY E III ,  SUSSER MARK

IPC: B04B11/04 ,  A61K35/14 ,  A61M1/02 ,  A61M1/10 ,  A61M1/36 ,  B04B5/04 ,  B04B13/00 ,  C12M1/00 ,  C12M1/34 ,  F04B43/02 ,  F16J15/16 ,  F16L39/00 ,  G01J1/04 ,  G01N21/03 ,  G01N21/27 ,  G01N33/48 ,  G01N35/00

CPC classification number: B04B7/08 ,  A61M1/0222 ,  A61M1/3687 ,  A61M1/3692 ,  A61M1/3693 ,  A61M1/3695 ,  A61M1/3696 ,  A61M1/3698 ,  A61M2202/0429 ,  A61M2205/128 ,  A61M2205/3393 ,  B04B5/0428 ,  B04B5/0442 ,  G01N2035/00217 ,  Y10S277/907 ,  Y10T137/87249 ,  A61M2202/0064

Abstract: The present invention is a method or an apparatus for interactively processing biological cells maintained in a sterile environment. Furthermore, the invention provides a superior system for enzymatically converting red blood cells of groups A, B, or AB to group O. A system (10) for interactively processing biological cells maintained in a sterile environment includes a supply module (20), a cell module (12), a processing module (60), at set of conduits, a plurality of valves, a plurality of sensors and a control module (80). The supply module is constructed and arranged to provide selected amounts of process chemicals. The cell module includes a cell sensor for measuring the amount of the biological cells supplied for processing to the processing module. The processing module is constructed and arranged to process said biological cells. Several conduits connect the supply module, the cell module and the processing module in a sterile manner, and the valves control transfer of the biological cells and the process chemicals between the modules. The sensors are constructed and arranged to detect the transfer of the biological cells and the process chemicals in the conduits. The control module is operatively connected to the valves, the sensors and the processing module. The control module also receives data from the cells sensor and controls the transfer and the processing of the biological cells based on the cell sensor data. The system can adjust the processing algorithm based on the type of the processed cells or the cell amount provided for processing.

公开(公告)号：WO99001197A1

公开(公告)日：1999-01-14

申请号：PCT/US1998/012166

申请日：1998-06-22

IPC: A61K35/14 ,  A61M1/36 ,  A61M1/38 ,  B01D21/26 ,  B04B5/04 ,  B04B11/04

CPC classification number: B01D21/302 ,  A61M1/3693 ,  A61M1/3696 ,  A61M1/38 ,  B01D21/262 ,  B01D2221/10 ,  B04B5/0442 ,  B04B2005/045

Abstract: Systems and methods rotate a chamber (14), in which whole blood is centrifugally separated into an interface between the packed red blood cells and the plasma constituent. The interface carries platelets and mononuclear cells. The systems and methods include an interface control unit which is operative in a first state to enable removal of platelet-poor plasma (PPP) in a first container for use as a diluting liquid, and in a second state, to retain mononuclear cells (MNC) in the chamber (14), while removing platelet-rich plasma (PRP) from the chamber (14), bypassing the platelet-poor collection container, thereby maintaining its platelet-poor character, and in a third state to enable the removal of mononuclear cells (MNC) from the chamber (14) in a path (T13) that leads to a second container, where the mononuclear cells are collected.

Abstract translation: 系统和方法旋转室（14），其中全血离心分离成包装的红细胞和血浆成分之间的界面。 界面携带血小板和单核细胞。 所述系统和方法包括接口控制单元，该接口控制单元在第一状态下工作以能够在用作稀释液体的第一容器中移除贫血小板血浆（PPP），并且在第二状态下保留单核细胞 ），同时从所述腔室（14）中去除富含血小板的血浆（PRP），绕过所述血小板贫乏的收集容器，从而保持其血小板不足的特征，并且在第三状态下能够移除 来自室（14）的单核细胞（MNC）在通向第二容器的路径（T13）中，其中收集单核细胞。