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    MICROFLUIDIC SYSTEM FOR REPRODUCING FUNCTIONAL UNITS OF TISSUES AND ORGANS IN VITRO
    77.
    发明申请
    MICROFLUIDIC SYSTEM FOR REPRODUCING FUNCTIONAL UNITS OF TISSUES AND ORGANS IN VITRO 审中-公开
    用于复制体外组织和器官功能单位的微流感系统

    公开(公告)号：WO2014052835A1

    公开(公告)日：2014-04-03

    申请号：PCT/US2013/062307

    申请日：2013-09-27

    Applicant: NORTIS, INC.

    Inventor： NEUMANN, Thomas ,  TOUROVSKAIA, Anna A. ,  FAUVER, Mark E. ,  KRAMER, Greg ,  ASP, Elizabeth ,  MANN, Henning

    IPC: C12M1/00 ,  A01N1/02

    CPC classification number: C12M21/08 ,  A01N1/0231 ,  A01N1/0247 ,  C12M23/16 ,  C12M25/14 ,  C12M29/06 ,  C12M29/10 ,  C12M33/00 ,  C12M33/04 ,  G01N33/5085

    Abstract: A microfluidic system for generating compartmentalized microenvironments of tissues and organs in vitro and for independently perfusing the compartments. A microfluidic device that includes at least a first perfusion path and a second separate perfusion path. The microfluidic device also has a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void can be populated with at least one cell type in such way that the cells are in direct contact with the matrix and the matrix is in fluidic connection exclusively with the second separate perfusion path.

    Abstract translation: 用于在体外产生组织和器官的分隔的微环境并用于独立灌注隔室的微流体系统。 一种微流体装置，其包括至少第一灌注路径和第二分离灌注路径。 所述微流体装置还具有包含基质的腔室，其中所述基质包围至少一个空腔，所述至少一个空腔的内腔与第一灌注路径专用流体连接，其中至少一个空隙可以以这种方式填充至少一种细胞类型 细胞与基质直接接触，并且基质仅与第二分离灌注路径流体连接。

    SYSTEMS AND METHODS FOR HARVESTING AND DEWATERING ALGAE
    78.
    发明申请
    SYSTEMS AND METHODS FOR HARVESTING AND DEWATERING ALGAE 审中-公开
    用于收获和脱水的系统和方法

    公开(公告)号：WO2013116357A1

    公开(公告)日：2013-08-08

    申请号：PCT/US2013/023878

    申请日：2013-01-30

    Applicant: ORIGINOIL, INC.

    Inventor： ECKELBERRY, Nicholas

    IPC: C02F3/32 ,  C02F1/48

    CPC classification number: A01G7/04 ,  A01G33/00 ,  A01H13/00 ,  C02F1/463 ,  C02F11/006 ,  C12M21/02 ,  C12M33/00 ,  C12M47/02 ,  C12N1/02 ,  C12N13/00 ,  Y02A40/88

    Abstract: An applied electrical field effects the harvesting of algae from a growth medium through increased interface potential between solvent and solute and the use of micron-sized bubbles of hydrogen and oxygen gas. The process and method makes use of strategically placed bipolar electrode plates that generate hydrogen and oxygen gas. Micro bubbles of the gas flocculate the biomass out of solution concurrently clarifying the water for re-use in an algae growth system. The flocked algae can then be processed for use in applications which require a chemical-free and dewatered product such as required for bio-fuels, pharmaceuticals or food.

    Abstract translation: 施加的电场通过增加溶剂和溶质之间的界面电位以及使用微米级的氢气和氧气泡来影响从生长介质中收获藻​​类。 该方法和方法利用产生氢气和氧气的策略性放置的双极电极板。 气体的微气泡将生物质从溶液中絮凝，同时澄清藻类生长系统中的水再利用。 然后可以将植绒的藻类加工成用于需要无化学物质和脱水产品的应用，例如生物燃料，药物或食品所需的。

    SYSTEM AND METHOD FOR TISSUE CONSTRUCTION USING AN ELECTRIC FIELD APPLICATOR
    79.
    发明申请
    SYSTEM AND METHOD FOR TISSUE CONSTRUCTION USING AN ELECTRIC FIELD APPLICATOR 审中-公开
    使用电场应用器的组织结构的系统和方法

    公开(公告)号：WO2013019814A3

    公开(公告)日：2013-08-01

    申请号：PCT/US2012049056

    申请日：2012-07-31

    Applicant: TOKYO ELECTRON LTD ,  BRCKA JOZEF

    Inventor： BRCKA JOZEF

    IPC: C12M3/00 ,  B03C5/00 ,  C12M1/26 ,  H01J37/32

    CPC classification number: C23C16/50 ,  A61F2/00 ,  B03C5/005 ,  B03C5/026 ,  C12M3/00 ,  C12M21/08 ,  C12M33/00 ,  C23C16/04 ,  C23C16/48 ,  H01J37/32009 ,  H01J37/32697 ,  H01L21/02612 ,  H01L21/67011

    Abstract: A method and apparatus are provided for constructing tissue from cells or other objects by application of temporally and spatially controlled electric fields. Electric field applicators expose a substrate (32) to the electric field controlled to affect the processing medium (28) to achieve a processing effect on the construction of tissue on the substrate (32). Electrical bias is selected to interact with dipole properties of the medium (28) to control the movement of suspended dielectrophoretic cells or other particles in the medium (28) or at the substrate (32). The motion of suspended particles may be affected to cause suspended particles of different properties to follow different paths in the processing medium (28), which may be used to cause the suspended particles to be sorted. The processing medium (28) and electrical bias may be selected to affect the structure, or orientation, of one or more layers on the substrate (32).

    Abstract translation: 提供了一种用于通过应用时间和空间控制的电场从电池或其他物体构造组织的方法和装置。 电场施加器将衬底（32）暴露于被控制以影响处理介质（28）的电场，以实现对衬底（32）上的组织的构造的处理效果。 选择电偏压以与介质（28）的偶极子性质相互作用，以控制介质（28）中或基板（32）上的悬浮介电泳细胞或其它颗粒的运动。 悬浮颗粒的运动可能受到影响，导致不同性质的悬浮颗粒遵循处理介质（28）中不同的路径，可用于引起悬浮颗粒的分选。 可以选择处理介质（28）和电偏压来影响衬底（32）上的一个或多个层的结构或取向。

    SYSTEMS AND DEVICES FOR RESTRAINING A MICRO-OBJECT AND ASSOCIATED METHODS
    80.
    发明申请
    SYSTEMS AND DEVICES FOR RESTRAINING A MICRO-OBJECT AND ASSOCIATED METHODS 审中-公开
    用于限制微观对象和相关方法的系统和设备

    公开(公告)号：WO2013059717A1

    公开(公告)日：2013-04-25

    申请号：PCT/US2012/061185

    申请日：2012-10-19

    Applicant: NANOINJECTION TECHNOLOGIES, L.L.C. ,  ATEN, Quentin T.

    Inventor： ATEN, Quentin T.

    IPC: C12N15/87 ,  C12M1/00 ,  C12N15/89 ,  C12Q1/02

    CPC classification number: C12M21/06 ,  C12M23/12 ,  C12M33/00 ,  C12M35/00 ,  Y10T29/49826

    Abstract: Systems, devices, and methods for restraining a micro-object are provided. In one aspect, a micro-object restraint device is provided having a micro-barrier structure coupled to a support substrate, where the micro-barrier structure has two micro-object holding regions. Each micro-object holding region includes a micro-object receiving opening defined in the micro-barrier structure, a micro-object impeding opening at an internal region of the micro-barrier structure, and at least two contact points positioned adjacent to the micro-object impeding opening and oriented to contact and impede a micro-object at the micro-object opening. The two micro-object holding regions abut one another and the micro-object openings from the two micro-object holding regions are continuous.

    Abstract translation: 提供了用于限制微物体的系统，设备和方法。 在一个方面，提供一种具有耦合到支撑衬底的微阻挡结构的微物体约束装置，其中微阻挡结构具有两个微物体保持区域。 每个微物体保持区域包括限定在微阻挡结构中的微物体接收开口，在微阻挡结构的内部区域处的微物体阻挡开口，以及至少两个接触点， 物体阻止开口并且定向成在微物体开口处接触和阻碍微物体。 两个微物体保持区彼此邻接，并且来自两个微物体保持区域的微物体开口是连续的。

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