公开(公告)号：WO01067090A1

公开(公告)日：2001-09-13

申请号：PCT/US2001/001119

申请日：2001-01-11

IPC: G01N30/02 ,  G01N30/26 ,  G01N30/64 ,  G01N30/88 ,  G01N30/96

CPC classification number: G01N30/96 ,  G01N30/02 ,  G01N2030/965 ,  B01D15/422

Abstract: Apparatus for analysis of ionic species in an aqueous liquid stream comprising: (a) a chromatographic separator (10) including medium for separating said ionic species and having an inlet and outlet (b) a source of an aqueous eluent solution comprising electrolyte in fluid communication with said chromatographic separator; (c) a membrane suppressor (12) comprising a chromatography affluent flow channel separated by an ion exchange membrane from a regenerant flow channel having an inlet and an outlet; (d) a container (26) including an inlet and an outlet, said container outlet being in fluid communication with said regenerant flow channel; (e) a regenerant liquid reservoir (28) in said container of a different chemical composition than said chromatography affluent; (f) a detector (16) for detecting ionic species in fluid communication with said chromatography effluent flow channel; and (g) a recycle conduit (30) for the flow of effluent from said detector to said container inlet.

Abstract translation: 用于分析水性液体流中的离子物质的装置，包括：（a）色谱分离器（10），其包括用于分离所述离子物质的介质，并具有入口和出口（b）包含流体连通的电解质的含水洗脱液源 与所述色谱分离器; （c）膜抑制器（12），包括由具有入口和出口的再生剂流动通道由离子交换膜分离的色谱富余流动通道; （d）包括入口和出口的容器（26），所述容器出口与所述再生剂流动通道流体连通; （e）所述容器中与所述色谱富集物不同的化学成分的再生液体储存器（28）; （f）检测器（16），用于检测与所述色谱流出物流动通道流体连通的离子物质; 和（g）用于将流出物从所述检测器流出到所述容器入口的循环管道（30）。

公开(公告)号：WO01046687A2

公开(公告)日：2001-06-28

申请号：PCT/US2000/029387

申请日：2000-10-25

IPC: G01N33/50 ,  B01D15/16 ,  B01D15/36 ,  B01J20/281 ,  B01J20/285 ,  C12N15/10 ,  C12Q1/68 ,  G01N30/04 ,  G01N30/20 ,  G01N30/24 ,  G01N30/26 ,  G01N30/30 ,  G01N30/32 ,  G01N30/34 ,  G01N30/54 ,  G01N30/74 ,  G01N30/80 ,  G01N30/82 ,  G01N30/86 ,  G01N30/88 ,  G01N30/96

CPC classification number: C12N15/101 ,  B01D15/166 ,  B01D15/366 ,  C12Q1/6869 ,  G01N30/30 ,  G01N30/96 ,  G01N2030/8827 ,  C12Q2565/137 ,  C12Q2527/107

Abstract: In an extensive Matched Ion Polynucleotide Chromatography (MIPC) system and method, and the computer programs or software associated therewith, the system provides automated options for sample selection, mobile phase gradient selection and control, column and mobile phase temperature control, and fragment collection for a wide variety of MIPC separation processes. MIPC separation processes can be applied to effect size-based separation of DNA fragments, mutation detection, DNA fragment purification, PCR process monitoring and other novel processes. This invention is directed to the system and software which automates many of these procedures, facilitating use of the system to achieve complex separation methods. In one embodiment of the invention, a user specifies a size range of double stranded DNA fragment(s) in a mixture, the software calculates a solvent gradient to elute the fragment(s), and the system performs the chromatographic separation using the calculated gradient. In an embodiment useful in DNA mutation detection, a user specifies the base sequence of a wild type DNA molecule, the software calculates a temperature for partially denaturing heteroduplex and homoduplex molecules of the DNA in a mixture, the software calculates a solvent gradient to elute the fragments, and the system performs the chromatographic separation using the calculated gradient and temperature.

Abstract translation: 在广泛的匹配离子多核苷酸色谱（MIPC）系统和方法以及与其相关的计算机程序或软件中，系统提供用于样品选择，流动相梯度选择和控制，色谱柱和流动相温度控制以及片段收集的自动选择 各种MIPC分离过程。 可以应用MIPC分离过程来实现基于大小的DNA片段分离，突变检测，DNA片段纯化，PCR过程监控等新方法。 本发明涉及自动化许多这些程序的系统和软件，便于使用该系统来实现复杂的分离方法。 在本发明的一个实施方案中，用户指定混合物中双链DNA片段的大小范围，软件计算溶剂梯度以洗脱片段，并且系统使用计算的梯度进行色谱分离 。 在DNA突变检测中有用的实施方案中，用户指定野生型DNA分子的碱基序列，软件计算混合物中DNA的部分变性异源双链体和同源双链体分子的温度，软件计算溶剂梯度洗脱 片段，系统使用计算的梯度和温度进行色谱分离。

公开(公告)号：WO0037932A2

公开(公告)日：2000-06-29

申请号：PCT/US9931017

申请日：1999-12-23

Applicant: CPBD INC

Inventor： MORGAN LING HAN ,  PIDGEON CHARLES ,  ROOKE NADEGE ,  YIN JIANMING

IPC: G01N30/02 ,  G01N30/34 ,  G01N30/52 ,  G01N30/72 ,  G01N30/86 ,  G01N30/89 ,  G01N30/96

CPC classification number: G01N30/96 ,  G01N30/02 ,  G01N30/34 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/8637 ,  G01N30/89 ,  G01N2030/527 ,  Y10T436/16

Abstract: This invention provides a rapid, high throughput method for simultaneously measuring biologically significant physicochemical characteristics for multiple compounds. More specifically, this invention is directed to the measurement and use of binding affinities of multiple compounds with a surface under controlled conditions to calculate biologically significant physicochemical values.

Abstract translation: 本发明提供了一种用于同时测量多种化合物的生物学显着物理化学特性的快速，高通量的方法。 更具体地，本发明涉及在受控条件下测量和使用多种化合物与表面的结合亲和力以计算生物学显着的物理化学值。

公开(公告)号：WO99044054A1

公开(公告)日：1999-09-02

申请号：PCT/US1999/003427

申请日：1999-02-17

IPC: G01N30/02 ,  G01N30/60 ,  G01N30/96

CPC classification number: G01N30/96 ,  G01N2030/965

Abstract: An electrolytic suppressor (22) including (a) a suppressor bed (26) of ion exchange resin, (b) an electrode chamber (44) adjacent the suppressor, (c) a first electrode (42) in the electrode chamber, (d) a barrier (40) separating the suppressor bed from the first electrode chamber preventing significant liquid flow but permitting transport of ions only of the same charge as the suppressor bed resin, (e) a second electrode (36) in electrical communication with the resin bed, and (f) a recycle conduit (30, 32, 34, 38, 48) between the suppressor outlet port and said electrode chamber. The second electrode may be in contact with the ion exchange resin in the suppressor or located in second electrode chamber. For anion analysis, the method of using the apparatus includes: (a) flowing an aqueous liquid sample stream containing anions to be detected and cation hydroxide through the separator bed, (b) flowing the aqueous effluent from the separator bed through the flow-through suppressor, (c) flowing the effluent liquid from the suppressor past a detector, (d) recycling said liquid effluent from the detector through a cathode chamber proximate to the suppressor bed and separated by the first barrier, and (e) applying an electrical potential between the cathode and the anode. Water is electrolyzed at the anode to cause cations on the cation exchange resin to electromigrate toward the barrier and to be transported across the barrier toward the cathode while water in the cathode chamber is electrolyzed to generate hydroxide ions which combine with the transported cations to form cation hydroxide in the cathode chamber.

Abstract translation: 一种电解抑制器（22），包括（a）离子交换树脂的抑制床（26），（b）邻近所述抑制器的电极室（44），（c）所述电极室中的第一电极（d） ）将抑制床与第一电极室分隔开的阻挡层（40），其防止显着的液体流动，但允许仅传输与抑制床树脂相同电荷的离子，（e）与树脂电连通的第二电极（36） 床和（f）在抑制器出口和所述电极室之间的循环管道（30,32,34,38,48）。 第二电极可以与抑制器中的离子交换树脂接触或位于第二电极室中。 对于阴离子分析，使用该装置的方法包括：（a）使含有待检测的阴离子和阳离子氢氧化物的含水液体样品流通过分离床，（b）使来自分离器床的水性流出物流过流过 （c）将来自抑制器的流出液体流过检测器，（d）将来自检测器的所述液体流出物再循环通过靠近抑制床的阴极室并被第一屏障分离，以及（e）施加电势 在阴极和阳极之间。 在阳极处电解水以使阳离子交换树脂上的阳离子电致化学势垒，并将阴极室内的水转移至阴极，同时电解阴极室内的水，以产生氢氧化物离子，与运送的阳离子结合形成阳离子 氢氧化物在阴极室中。

公开(公告)号：WO99038595A1

公开(公告)日：1999-08-05

申请号：PCT/US1999/001757

申请日：1999-01-28

IPC: G01N30/26 ,  B01D15/08 ,  B01J39/26 ,  B01J41/20 ,  C25B1/16 ,  C25B1/22 ,  G01N30/02 ,  G01N30/34 ,  G01N30/60 ,  G01N30/96

CPC classification number: G01N30/96 ,  C25B1/16 ,  C25B1/22 ,  G01N30/02 ,  G01N30/34 ,  G01N2030/965 ,  Y10T436/25 ,  B01D15/36 ,  B01D15/426

Abstract: Method and apparatus for generating an acid or base, e.g., for chromatographic analysis of ions. For generating a base, a cation source, such as a salt, cation hydroxide or cation exchange resin, is provided in a cation reservoir (10) separated from a base generation chamber (12) by a permselective barrier (14). An aqueous stream (21) flows through the base generation chamber (12) containing a cation exchange material (19). An electric potential is applied between an anode (16) in the cation source reservoir (10) and a cathode (18) in the base generation chamber (12) to electrolytically generate hydroxide ions and cause cations in the reservoir (10) to electromigrate through the barrier (14) toward the cathode (18) to combine with the migrated cations to form cation hydroxide, which flows from the base generation chamber (12).

Abstract translation: 用于产生酸或碱的方法和装置，例如用于离子的色谱分析。 为了产生碱，阳离子源（例如盐，阳离子氢氧化物或阳离子交换树脂）被提供在通过选择性渗透屏障（14）从基底产生室（12）分离的阳离子储存器（10）中。 水流（21）流过含有阳离子交换材料（19）的基底产生室（12）。 在阳离子源储存器（10）中的阳极（16）和基底产生室（12）中的阴极（18）之间施加电位以电解产生氢氧根离子并使贮存器（10）中的阳离子电致 所述阻挡层（14）朝向阴极（18）与迁移的阳离子结合以形成从基底产生室（12）流出的阳离子氢氧化物。

公开(公告)号：WO1996028009A2

公开(公告)日：1996-09-19

申请号：PCT/US1996002949

申请日：1996-02-29

Applicant: DIONEX CORPORATION

Inventor： DIONEX CORPORATION ,  RABIN, Steven, B. ,  SMALL, Hamish ,  RIVIELLO, John, M.

IPC: G01N30/02 ,  G01N30/60 ,  G01N30/96

CPC classification number: G01N30/96 ,  G01N2030/965

Abstract: A method and apparatus using suppression of eluents for the analysis of anions or cations in ion chromatography. The invention relates to the intermittent use of the electric field during electrochemical suppression to minimize background noise during detection of the ionic species caused by the application of an electric field using an electrolytic membrane suppressor. Using this intermittent electrochemical suppression system allows the detection of the analyte in the absence of the electric field. The system finds particular benefit in reducing background noise created during electrochemical suppression, and in reducing the baseline interference when analyzing organic solvent-containing samples.

Abstract translation: 在离子色谱法中使用抑制洗脱液进行阴离子或阳离子分析的方法和装置。 本发明涉及在电化学抑制期间间歇地使用电场，以使由使用电解膜抑制器的电场施加引起的离子物质检测期间的背景噪声最小化。 使用这种间歇电化学抑制系统允许在没有电场的情况下检测分析物。 该系统在减少电化学抑制期间产生的背景噪声以及在分析含有机溶剂的样品时降低基线干扰方面具有特别的益处。

公开(公告)号：WO1996027791A1

公开(公告)日：1996-09-12

申请号：PCT/US1996002948

申请日：1996-02-29

Applicant: DIONEX CORPORATION

Inventor： DIONEX CORPORATION ,  POHL, Christopher, A. ,  SIRIRAKS, Archava ,  SLINGSBY, Rosanne, W. ,  DHILLON, Harpreet S.

IPC: G01N30/02

CPC classification number: G01N30/96 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N2030/146 ,  Y10T436/173076 ,  Y10T436/19 ,  Y10T436/193333 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255 ,  B01D15/36 ,  B01D15/422

Abstract: Selected polyvalent anions or cations are removed from a liquid sample stream as a pretreatment for the analysis of the liquid sample stream. A stream containing anions of interest, precipitable anions (e.g. sulfate ions) to be removed and an added displacing salt having a polyvalent cation flow through a cation exchange resin bed having precipitable exchangeable polyvalent ions (e.g. barium ions). The polyvalent cation (e.g. calcium) of the displacing salt exchanges with the cation of the resin, which reacts with the precipitable anion to form a precipitate which is retained in the bed. The anions of interest are subsequently separated and analyzed. The invention is also applicable to the removal of precipitable cations (e.g. barium ions) by reversing the roles of the anions and cations.

Abstract translation: 选择的多价阴离子或阳离子作为用于分析液体样品流的预处理从液体样品流中去除。 含有感兴趣的阴离子，待除去的可沉淀的阴离子（例如硫酸根离子）的流和通过具有可沉淀的可交换的多价离子（例如钡离子）的阳离子交换树脂床的具有多价阳离子流的添加的置换盐。 置换盐的多价阳离子（例如钙）与树脂的阳离子交换，其与可沉淀的阴离子反应以形成保留在床中的沉淀物。 随后分离和分析感兴趣的阴离子。 本发明也适用于通过逆转阴离子和阳离子的作用来除去可沉淀的阳离子（例如钡离子）。

公开(公告)号：WO1993007481A1

公开(公告)日：1993-04-15

申请号：PCT/US1992006076

申请日：1992-07-21

Applicant: MARATHON OIL COMPANY

Inventor： MARATHON OIL COMPANY ,  ATWOOD, Sonia, E.

IPC: G01N30/02

CPC classification number: G01N30/96 ,  G01N30/02 ,  G01N2030/8859 ,  B01D15/36

Abstract: A method for identification and quantification of sulfonation by-products by ion chromatography. The method utilizes two eluants and preferably three eluants to identify and quantify the by-products on a single chromatographic run.

公开(公告)号：WO1981001245A1

公开(公告)日：1981-05-14

申请号：PCT/US1980001411

申请日：1980-10-22

Applicant: ISOLAB INC

Inventor： ISOLAB INC ,  ACUFF K ,  VOLK M ,  ROSENTHAL M

IPC: B01D15/08

CPC classification number: H01M2/1235 ,  B01D15/08 ,  G01N30/02 ,  G01N30/6004 ,  G01N30/96 ,  G01N33/66 ,  H01M2/0235 ,  H01M2/1282 ,  B01D15/363 ,  B01D15/362

Abstract: Methods and microcolumns (20) to determine a numerical percentage value as a diagnostic indicator of the blood sugar condition of a specific person. A whole blood sample (10) is lysed and a test sample thereof is placed on a microchromatographic column bed of ion exchange material particles (27). The column bed is characterized by having no cyanide therein. The column bed comprises an equilibrated suspension of particles selected from the class consisting of CarXH and CarYOH, where "Car" represents an inert substrate for carrying ionizable groups X- providing dissociated cations H+ and ionizable groups Y+ providing anions OH-. Fractions (31, 32) of various hemoglobin species in the test sample are preferentially eluted or desorbed from the column bed by buffer and wash solutions characterized by having no cyanide therein. Amounts of hemoglobin species in the eluate fractions are detected and measured by spectrometric (color) analysis (40). A mathematical computation using integer factors corresponding to amounts of various hemoglobin species produces the numerical percentage value.

公开(公告)号：WO2016188905A1

公开(公告)日：2016-12-01

申请号：PCT/EP2016/061440

申请日：2016-05-20

Applicant: CSL BEHRING RECOMBINANT FACILITY AG

Inventor： SCHULTE, Stefan ,  BELTZ, Hans-Wilhelm ,  PESTEL, Sabine ,  WEIMER, Thomas ,  PELZING, Matthias

IPC: C07K14/755

CPC classification number: A61K38/37 ,  A61K38/1741 ,  A61P7/04 ,  C07K14/473 ,  C07K14/755 ,  C07K2319/02 ,  C07K2319/31 ,  G01N30/96

Abstract: The present invention provides modified von Willebrand Factor molecules, methods for their preparation and uses thereof. The invention further provides pharmaceutical compositions for treating coagulation disorders.

Abstract translation: 本发明提供了修饰的血管性血友病因子分子，其制备方法和用途。 本发明还提供了用于治疗凝血障碍的药物组合物。