公开(公告)号：WO9502058A3

公开(公告)日：1995-06-01

申请号：PCT/US9407886

申请日：1994-07-08

申请人： ECOGEN INC

发明人： BAUM JAMES A

IPC分类号： A01M1/20 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N9/00 ,  C12N15/00 ,  C12N15/09 ,  C12N15/75 ,  C12R1/07 ,  C12R1/19 ,  A01H5/00 ,  C12N15/32 ,  C12N15/52

CPC分类号： C12N9/93 ,  A01N63/00 ,  C07K14/325 ,  C12N15/75 ,  Y10S435/832

摘要： A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t..

公开(公告)号：WO9502694A3

公开(公告)日：1995-03-30

申请号：PCT/US9407902

申请日：1994-07-13

申请人： MYCOGEN CORP

发明人： HICKLE LESLIE A ,  PAYNE JEWEL

IPC分类号： C07K14/32 ,  C07K14/325 ,  C12N15/32 ,  A01N63/00 ,  C12N1/21

CPC分类号： C07K14/32 ,  C07K14/325 ,  Y10S530/825

摘要： Methods and compositions for the control of pests of the family Calliphoridae are described. Specifically, Bacillus thuringiensis (B.t.) isolates having anti-calliphorid activity are disclosed. Also described are recombinant hosts which express B.t. genes coding for pesticidal toxins. The B.t. isolates and recombinant proteins are shown to be useful in a method for controlling calliphorids including screw-worms and the sheep blowfly.

公开(公告)号：WO9425612A3

公开(公告)日：1995-01-05

申请号：PCT/FR9400525

申请日：1994-05-05

申请人： PASTEUR INSTITUT ,  AGRONOMIQUE INST NAT RECH ,  LERECLUS DIDIER ,  AGAISSE HERVE

发明人： LERECLUS DIDIER ,  AGAISSE HERVE

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/75 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  C12N15/32 ,  C12N15/56 ,  C12N15/90

CPC分类号： C07K14/325 ,  C12N15/75

摘要： The invention relates to sequences of nucleotides of bacteria, particularly Gram+ bacteria such as bacteria of the Bacillus type and more particularly sequences of nucleotides of the gene CryIIIA for the control of the expression of DNA sequences in a cellular host. The invention relates particularly to an expression system comprising a DNA sequence susceptible of being involved in the control of the expression of a coding sequence of nucleotides. Said DNA sequence comprises a promoter, as well as a sequence of nucleotides called 'downstream region', situated between the promoter and the coding sequence of the gene to be expressed, and susceptible of acting at the post-transcriptional level during the expression of the gene. Preferably, the downstream region comprises a nucleotide sequence S2 comprising an essentially complementary region at the extremity 3' of the RNA 16S of the ribosomes of Bacillus type bacteria.

公开(公告)号：WO9416079A3

公开(公告)日：1994-10-13

申请号：PCT/US9312682

申请日：1993-12-30

申请人： MYCOGEN CORP

发明人： PAYNE JEWEL M ,  NARVA KENNETH E

IPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  C12P1/04 ,  C12P1 ,  C12P21/02

CPC分类号： C07K14/325 ,  A01N63/00

摘要： Disclosed and claimed are toxins and genes from Bacillus thuringiensis strains designated PS80JJ1, PS158D5, PS167P, PS169E, PS177F1, PS177G, PS204G4, PS204G6 which can be used to control corn rootworm. Mutants which retain the activity of the parent strain can be used to control the pest. Further, isolated spores or purified toxins from these isolates can be used to control corn rootworm. Genes encoding δ-endotoxins can be removed from these strains using standard well-known techniques, and transferred to other hosts. Expression of the δ-endotoxin in such hosts results in control of corn rootworm larvae.

公开(公告)号：WO9315206A3

公开(公告)日：1994-01-06

申请号：PCT/US9300966

申请日：1993-02-01

申请人： MYCOGEN CORP

发明人： MICHAELS TRACY E ,  FONCERRADA LOUIS ,  NARVA KENNETH E

IPC分类号： C12N15/09 ,  A01N63/00 ,  A01N63/02 ,  C07K14/195 ,  C07K14/325 ,  C07K14/41 ,  C12N1/21 ,  C12N15/32 ,  C12P21/02 ,  C12R1/38 ,  C12N15/82 ,  C12P1/04

CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  Y10S435/832

摘要： Certain isolates of Bacillus thuringiensis (B.t.) have been found to have activity against scarab pests. These isolates are designated B.t. PS86B1, B.t. PS43F and B.t. PS50C. These isolates, or transformed hosts containing the gene expressing a scarab-active toxin obtained from the isolates, can be used to control scarab-active pests, e.g., masked chafer, Cyclocephala sp., June bettle, Cotinis sp., northern masked chafer, Cyclocephala borealis, Japanese beetle, Popillia japonica, and Pasadena masked chafer, Cyclocephala pasadenae, in various environments.

公开(公告)号：WO1993003154A1

公开(公告)日：1993-02-18

申请号：PCT/US1992006404

申请日：1992-07-31

申请人： MYCOGEN CORPORATION ,  KUBOTA CORPORATION

发明人： MYCOGEN CORPORATION ,  KUBOTA CORPORATION ,  OHBA, Michio ,  IWAHANA, Hidenori ,  SATO, Ryoichi ,  SUZUKI, Nobukazu ,  OGIWARA, Katsutoshi ,  SAKANAKA, Kazunobu ,  HORI, Hidetaka ,  ASANO, Shouji ,  KAWASUGI, Tadaaki

IPC分类号： C12N15/32

CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Bacillus thuringiensis serovar japonensis strain Buibui (FERM BP-3465) belonging to Bacillus thuringiensis serovar japonensis and capable of producing insecticidal toxin proteins to kill coloepterous larvae, and an insecticide containing, as an effective ingredient, the toxin proteins produced.

摘要翻译： 属于苏云金芽孢杆菌血清型粳稻的苏云金芽孢杆菌血清型粳稻品种Buibui（FERM BP-3465），能够产生杀虫性蛋白质以杀灭幼嫩幼虫，以及含有作为有效成分的杀虫剂，产生毒素蛋白质。

公开(公告)号：WO1992013954A1

公开(公告)日：1992-08-20

申请号：PCT/US1992000040

申请日：1992-01-03

申请人： ECOGEN INC.

发明人： ECOGEN INC. ,  DONOVAN, William, P. ,  RUPAR, Mark, J. ,  SLANEY, Annette, C.

IPC分类号： C12N15/32

CPC分类号： A01N63/00 ,  C07K14/325

摘要： A Bacillus thuringiensis strain isolate, designated EG5144, exhibits insecticidal activity against coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica. A novel toxin gene in B.t. strain EG5144 produces an irregularly shaped insecticidal crystal protein of approximately 70 kDa that is toxic to coleopteran insects. The cryIII-type gene (SEQ ID NO:1), designated as the cryIIIC(b) gene, has a nucleotide base sequence illustrated in Figure 1.

摘要翻译： 命名为EG5144的苏云金芽孢杆菌菌株分离株表现出对鞘翅目昆虫的杀虫活性，包括科罗拉多马铃薯甲虫和Diabrotica属的昆虫。 一种新型毒素基因 菌株EG5144产生约70kDa的不规则形状的杀虫晶体蛋白，对鞘翅目昆虫有毒。 称为cryIIIC（b）基因的cryIII型基因（SEQ ID NO：1）具有图1所示的核苷酸碱基序列。

公开(公告)号：WO9116434A3

公开(公告)日：1991-12-12

申请号：PCT/US9102560

申请日：1991-04-15

申请人： ECOGEN INC

发明人： GAWRON-BURKE M CYNTHIA ,  CHAMBERS JUDITH A ,  GONZALEZ JOSE M JR

IPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/32 ,  A01N63/02 ,  C12Q1/68

CPC分类号： A01N63/00 ,  C07K14/325

摘要： Two purified and isolated cryI-type genes were obtained from a novel B.t. strain. One gene, designated cryIF (SEQ ID NO:1), has a nucleotide base sequence coding for the amino acid sequence illustrated in Figure 1. The 134 kDa crystal protein, designated CryIF (SEQ ID NO:2), produced by this gene is toxic to European corn borer larvae and other lepidopteran insects. The second gene, designated cryIX, produces a crystal portein of about 81 kDa, designated CryIX, that is also toxic to lepidopteran insects.

公开(公告)号：WO1990015139A1

公开(公告)日：1990-12-13

申请号：PCT/EP1990000905

申请日：1990-05-30

申请人： PLANT GENETIC SYSTEMS N.V. ,  VAN MELLAERT, Herman ,  BOTTERMAN, Johan ,  VAN RIE, Jeroen ,  JOOS, Henk

发明人： PLANT GENETIC SYSTEMS N.V.

IPC分类号： C12N15/32

CPC分类号： A01N65/08 ,  A01N65/00 ,  A01N65/12 ,  A01N65/38 ,  C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  C12R1/075 ,  Y02A40/162 ,  A01N2300/00

摘要： Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.

公开(公告)号：WO1990002801A2

公开(公告)日：1990-03-22

申请号：PCT/EP1989001036

申请日：1989-09-05

申请人： PLANT GENETIC SYSTEMS, N.V. ,  BOTTERMAN, Johan ,  PEFEROEN, Marnix ,  HOFTE, Herman ,  JOOS, Henk

发明人： PLANT GENETIC SYSTEMS, N.V.

IPC分类号： C12N15/32

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： A plant cell transformed with all or part of a Bacillus thuringiensis gene coding for either a 130 or 132 kDa protoxin against Lepidoptera, preferably with a part of the gene coding just for the respective 62 or 60 kDa trypsin-digestion product of the protoxin. A plant, regenerated from the transformed cell, is resistant to Lepidoptera.

摘要翻译： 本发明涉及编码前毒素100kDa或130或132抗鳞翅目，优选仅用于摘要的编码基因的一部分与苏云金芽孢杆菌基因的全部或部分转化的植物细胞 原毒素的胰蛋白酶kDa 62或60。 来自转化细胞的再生植物对鳞翅目具有抗性。