公开(公告)号：WO1993002199A1

公开(公告)日：1993-02-04

申请号：PCT/FR1992000711

申请日：1992-07-20

申请人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ... ,  LERECLUS, Didier

发明人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ...

IPC分类号： C12N15/74

CPC分类号： C12N15/75 ,  C07K14/325

摘要： A recombinant vector capable of replicating in Gram positive bacteria and containing: a) a nucleotide concatenation I of Bacillus thuringiensis with a size of about 2.6 kb between sites BalI-HpaI, or any fragment included in said concatenation as long as its allows replication of the recombinant vector when under the control of a functional promoter in Gram positive bacteria, or any sequence which hybridizes with the complementary sequence of concatenation I or the above-mentioned fragment under highly stringent conditions, and b) at least one DNA sequence of interest inserted in phase with the above-mentioned concatenation.

公开(公告)号：WO1997015677A3

公开(公告)日：1997-05-01

申请号：PCT/FR1996001684

申请日：1996-10-28

申请人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ,  BRAVO, Alejandra ,  LERECLUS, Didier ,  AGAISSE, Hervé ,  SALAMITOU, Sylvie ,  SANCHIS, Vincent

发明人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

IPC分类号： C12N15/32

公开(公告)号：WO1994025612A2

公开(公告)日：1994-11-10

申请号：PCT/FR1994000525

申请日：1994-05-05

申请人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ,  LERECLUS, Didier ,  AGAISSE, Hervé

发明人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

IPC分类号： C12N15/75

CPC分类号： C07K14/325 ,  C12N15/75

摘要： The invention relates to sequences of nucleotides of bacteria, particularly Gram bacteria such as bacteria of the Bacillus type and more particularly sequences of nucleotides of the gene CryIIIA for the control of the expression of DNA sequences in a cellular host. The invention relates particularly to an expression system comprising a DNA sequence susceptible of being involved in the control of the expression of a coding sequence of nucleotides. Said DNA sequence comprises a promoter, as well as a sequence of nucleotides called "downstream region", situated between the promoter and the coding sequence of the gene to be expressed, and susceptible of acting at the post-transcriptional level during the expression of the gene. Preferably, the downstream region comprises a nucleotide sequence S2 comprising an essentially complementary region at the extremity 3' of the RNA 16S of the ribosomes of Bacillus type bacteria.

摘要翻译： 本发明涉及细菌的核苷酸序列，特别是革兰氏阳性细菌，例如芽孢杆菌属细菌，特别是用于控制细胞宿主中DNA序列表达的CryIIIA基因的核苷酸序列。 本发明特别涉及包含易受参与控制核苷酸编码序列表达的DNA序列的表达系统。 所述DNA序列包含启动子以及称为“下游区”的核苷酸序列，其位于启动子和待表达基因的编码序列之间，并且在表达期间易于在转录后水平作用 基因。 优选地，下游区域包含核苷酸序列S2，其包含在芽孢杆菌型细菌的核糖体的RNA 16S的末端3'处的基本上互补的区域。

公开(公告)号：WO1988009812A1

公开(公告)日：1988-12-15

申请号：PCT/FR1988000292

申请日：1988-06-09

申请人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE ,  SANCHIS, Vincent ,  LERECLUS, Didier ,  MENOU, Ghislaine ,  LECADET, Marguerite-Marie ,  MARTOURET, Daniel ,  DEDONDER, Raymond

发明人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

IPC分类号： C12N15/00

CPC分类号： C07K14/325

摘要： A nucleotide sequence coding for at least one part of the terminal N region of a polypeptide which exercises a specific toxic effect on Lepidoptera of the Noctuidae family, preferably on S.littoralis, is characterized by its capacity for hybridisation with a gene capable of expressing a polypeptide exercising a toxic effect on S.littoralis larvae.

摘要翻译： 编码对Noctuidae家族，优选在S.littoralis上对鳞翅目具有特异性毒性作用的多肽的末端N区的至少一部分的核苷酸序列的特征在于其与能够表达 对S.littoralis幼虫发挥毒性作用的多肽。

公开(公告)号：WO9715677A2

公开(公告)日：1997-05-01

申请号：PCT/FR9601684

申请日：1996-10-28

申请人： PASTEUR INSTITUT ,  AGRONOMIQUE INST NAT RECH ,  BRAVO ALEJANDRA ,  LERECLUS DIDIER ,  AGAISSE HERVE ,  SALAMITOU SYLVIE ,  SANCHIS VINCENT

发明人： BRAVO ALEJANDRA ,  LERECLUS DIDIER ,  AGAISSE HERVE ,  SALAMITOU SYLVIE ,  SANCHIS VINCENT

IPC分类号： C12N15/09 ,  A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/00 ,  C12N15/32 ,  C12R1/07 ,  C12N15/75 ,  A01N63/00 ,  C12N15/62 ,  C12N15/90

CPC分类号： C12R1/075 ,  C07K14/325 ,  C07K2319/00

摘要： The present invention relates particularly to a strain of Bacillus thuringiensis characterized in that it expresses the gene sigma E ( sigma E) and does not sporulate at all or litte sporulates or does not produce any viable spores. The invention also relates to a pesticide composition containing said strain of Bacillus thuringiensis.

摘要翻译： 本发明特别涉及苏云金芽孢杆菌菌株，其特征在于其表达基因σE（σE），并且根本不发生孢子形成或不产生任何可行的孢子。 本发明还涉及含有所述苏云金芽孢杆菌菌株的农药组合物。

公开(公告)号：WO9425612A3

公开(公告)日：1995-01-05

申请号：PCT/FR9400525

申请日：1994-05-05

申请人： PASTEUR INSTITUT ,  AGRONOMIQUE INST NAT RECH ,  LERECLUS DIDIER ,  AGAISSE HERVE

发明人： LERECLUS DIDIER ,  AGAISSE HERVE

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/75 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  C12N15/32 ,  C12N15/56 ,  C12N15/90

CPC分类号： C07K14/325 ,  C12N15/75

摘要： The invention relates to sequences of nucleotides of bacteria, particularly Gram+ bacteria such as bacteria of the Bacillus type and more particularly sequences of nucleotides of the gene CryIIIA for the control of the expression of DNA sequences in a cellular host. The invention relates particularly to an expression system comprising a DNA sequence susceptible of being involved in the control of the expression of a coding sequence of nucleotides. Said DNA sequence comprises a promoter, as well as a sequence of nucleotides called 'downstream region', situated between the promoter and the coding sequence of the gene to be expressed, and susceptible of acting at the post-transcriptional level during the expression of the gene. Preferably, the downstream region comprises a nucleotide sequence S2 comprising an essentially complementary region at the extremity 3' of the RNA 16S of the ribosomes of Bacillus type bacteria.