公开(公告)号：WO2008116368A1

公开(公告)日：2008-10-02

申请号：PCT/CN2008/000126

申请日：2008-01-17

Applicant: 中国农业科学院兰州兽医研究所 ,  中国农业科学院生物技术研究所 ,  柳纪省 ,  张志芳 ,  李志勇 ,  易咏竹 ,  殷相平 ,  白银梅 ,  李轶女 ,  李宝玉 ,  李学瑞 ,  杨彬 ,  兰喜 ,  沈桂芳

Inventor： 柳纪省 ,  张志芳 ,  李志勇 ,  易咏竹 ,  殷相平 ,  白银梅 ,  李轶女 ,  李宝玉 ,  李学瑞 ,  杨彬 ,  兰喜 ,  沈桂芳

IPC: C12N15/42 ,  C12N15/866 ,  C12N7/01 ,  C07K14/09 ,  A61K39/135 ,  A61P31/12 ,  C12R1/03

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2770/32122

Abstract: A method for expressing antigens of foot-and-mouth disease virus in insect in vivo by using recombinant baculovirus is disclosed, which comprises: cloning different genes combination of foot-and-mouth disease virus into baculovirus carrying vector to construct transfer vector, transfecting baculovirus with the transfer vector to proceed DNA recombination, and thus to get recombinant baculovirus, infecting insect host with the recombinant baculovirus, culturing the infected insect host to express the antigens of foot-and-mouth disease virus, and then collecting and purifying the expressed antigens. The preferred genes, baculovirus carrying vector, baculovirus and insect host are selected from P1-2A3C, ORF and VP1 genes of foot-and-mouth disease virus as shown in SEQ ID NO 1, 3 or 5, pVL1393, Bombyx mori Bm-NPV-ZJ8 and larvae or pupa of Bombyx mori, respectively.