公开(公告)号：WO2008116368A1

公开(公告)日：2008-10-02

申请号：PCT/CN2008/000126

申请日：2008-01-17

Applicant: 中国农业科学院兰州兽医研究所 ,  中国农业科学院生物技术研究所 ,  柳纪省 ,  张志芳 ,  李志勇 ,  易咏竹 ,  殷相平 ,  白银梅 ,  李轶女 ,  李宝玉 ,  李学瑞 ,  杨彬 ,  兰喜 ,  沈桂芳

Inventor： 柳纪省 ,  张志芳 ,  李志勇 ,  易咏竹 ,  殷相平 ,  白银梅 ,  李轶女 ,  李宝玉 ,  李学瑞 ,  杨彬 ,  兰喜 ,  沈桂芳

IPC: C12N15/42 ,  C12N15/866 ,  C12N7/01 ,  C07K14/09 ,  A61K39/135 ,  A61P31/12 ,  C12R1/03

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2770/32122

Abstract: A method for expressing antigens of foot-and-mouth disease virus in insect in vivo by using recombinant baculovirus is disclosed, which comprises: cloning different genes combination of foot-and-mouth disease virus into baculovirus carrying vector to construct transfer vector, transfecting baculovirus with the transfer vector to proceed DNA recombination, and thus to get recombinant baculovirus, infecting insect host with the recombinant baculovirus, culturing the infected insect host to express the antigens of foot-and-mouth disease virus, and then collecting and purifying the expressed antigens. The preferred genes, baculovirus carrying vector, baculovirus and insect host are selected from P1-2A3C, ORF and VP1 genes of foot-and-mouth disease virus as shown in SEQ ID NO 1, 3 or 5, pVL1393, Bombyx mori Bm-NPV-ZJ8 and larvae or pupa of Bombyx mori, respectively.

公开(公告)号：WO2008145000A1

公开(公告)日：2008-12-04

申请号：PCT/CN2008/000563

申请日：2008-03-21

Applicant: 中国农业科学院兰州兽医研究所 ,  柳纪省 ,  杨彬 ,  兰喜 ,  殷相平 ,  白银梅 ,  韩晓荣 ,  李学瑞 ,  李宝玉 ,  方玉萍 ,  李志勇

Inventor： 柳纪省 ,  杨彬 ,  兰喜 ,  殷相平 ,  白银梅 ,  韩晓荣 ,  李学瑞 ,  李宝玉 ,  方玉萍 ,  李志勇

IPC: C12N15/85 ,  C12N15/42 ,  A61K39/135 ,  A61P31/14

CPC classification number: A61K39/135 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/53 ,  A61K2039/552 ,  C12N15/1131 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2770/32134 ,  C12N2830/20 ,  C12N2840/203

Abstract: A foot-and-mouth disease virus double effect vaccine vector and the construction method and uses thereof are disclosed. The foot-and-mouth disease virus double -effective vaccine vector mainly consists of bicistronic expression vector sequence, and it comprises the antisense gene sequence that can bind to 5' non-coding region RNA of foot-and-mouth disease virus genome, and the gene sequence encoding complete VP1 structural protein of foot-and-mouth disease virus. Animal experiment shows that the foot-and-mouth disease virus double effective vaccine has double effects of gene therapy and gene immunization in the prevention and treatment of foot-and-mouth disease in animal.