公开(公告)号：WO2009134296A2

公开(公告)日：2009-11-05

申请号：PCT/US2009/001490

申请日：2009-03-09

Applicant: ARRYX, INC. ,  AKCAKIR, Osman

Inventor： AKCAKIR, Osman

IPC: G01B9/021

CPC classification number: G01B9/021 ,  G01N21/453 ,  G01N21/6456 ,  G01Q20/02 ,  G01Q30/04 ,  G01Q60/20 ,  G03H1/0443 ,  G03H2001/0033 ,  G03H2001/0077 ,  G03H2001/045 ,  G03H2001/0458 ,  G03H2223/23

Abstract: The present invention utilizes a holographic optical forcing array for dynamic cellular probing and diagnostics. A holographic optical trapping system generates optical forces on objects so that deformations thereof may be quantified. In one embodiment, digital holography is used to generate an interference pattern, and an analysis thereof determines the phase profile which yields a measurement of the objects' shape deformation using only one image. In another embodiment, phase-stepped holography allows the phase profile of an object to be measured using only one image, by using a holographic optical element to make phase-shifted replicas of the beam in space. In another embodiment, the optical forcing array applies optical forces to beads placed on the objects' surface, deforming the objects. The beads' position is determined by applying Mie theory, and analysis thereof yields the three dimensional position of the beads, and a measurement of the deformation displacement on the objects' surface.

Abstract translation: 本发明利用全息光学强制阵列进行动态细胞探测和诊断。 全息光学捕获系统在物体上产生光学力，从而可以量化其变形。 在一个实施例中，使用数字全息术来产生干涉图案，并且其分析确定相位轮廓，其仅使用一个图像来产生对象的形状变形的测量。 在另一个实施例中，相位阶全息术通过使用全息光学元件在空间中进行光束的相移复制，允许仅使用一个图像来测量物体的相位分布。 在另一个实施例中，光学强迫阵列将光学力施加到放置在物体表面上的珠子上，使物体变形。 通过应用米氏理论确定珠的位置，分析其产生珠的三维位置，并测量物体表面上的变形位移。

公开(公告)号：WO2009035623A1

公开(公告)日：2009-03-19

申请号：PCT/US2008/010601

申请日：2008-09-11

Applicant: ARRYX, INC. ,  CHAKRABARTY, Tania

Inventor： CHAKRABARTY, Tania

IPC: B01D11/00

CPC classification number: G01N33/5091 ,  G01N33/56966 ,  G01N2800/367

Abstract: The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted and unwanted objects, coating a surface of a sample holder with an antibody, placing an eluted sample on the sample holder, binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted and unwanted objects, separating the wanted objects, and performing PCR-based STR analysis on the wanted objects In one embodiment, holographic optical trapping is used to sort the wanted objects In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis

Abstract translation: 本发明涉及一种分类物体的方法和装置，包括：提供具有想要和不想要的物体的样品，用抗体涂覆样品架的表面，将洗脱的样品放置在样品架上，将所需物体中的抗原结合 与样品架表面上的抗体一起将物体分类为有用和不需要的物体，分离所需物体，并对所需物体进行基于PCR的STR分析。在一个实施例中，全息光学捕获用于对所需物体进行分类 在其他实施方案中，所需物体是精子，抗体是人精子特异性抗体，并且PCR是基于单细胞PCR的STR分析

公开(公告)号：WO2008005448A2

公开(公告)日：2008-01-10

申请号：PCT/US2007/015379

申请日：2007-07-03

Applicant: ARRYX, INC. ,  KNUTSON, Christopher

Inventor： KNUTSON, Christopher

IPC: C12M3/00

CPC classification number: B01J19/0046 ,  B01J2219/00382 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00617 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00745 ,  Y10T428/24893 ,  Y10T428/2982

Abstract: Nanoscale masking using particles patterned on a substrate include assembling particles into a pattern on a first substrate; contacting the particles with a second substrate; adding blocking molecules while the particles are in contact, such that blocking molecules bind to portions of the second substrate not in contact with the particles; and separating the substrates, yielding a functionalized substrate having blocking molecules bound thereto. Nanoscale printing methods include assembling particles into a desired pattern on a first substrate; contacting a print material with the particles such that at least a portion of the print material binds to the particles on the first substrate; removing the first substrate having particles thereon from unbound print material; contacting the particles having print material bound thereto with a second substrate such that at least a portion of the print material binds to the second substrate; and separating the substrates, yielding a printed substrate.

Abstract translation: 使用在基板上图案化的颗粒的纳米级掩模包括将颗粒组装成第一基板上的图案; 使颗粒与第二底物接触; 在颗粒接触的同时加入阻挡分子，使得阻挡分子与不与颗粒接触的第二基底的部分结合; 并分离底物，产生具有与其结合的阻断分子的官能化底物。 纳米级印刷方法包括在第一基底上将颗粒组装成所需图案; 使打印材料与颗粒接触，使得至少一部分印刷材料与第一基底上的颗粒结合; 从未结合的印刷材料中除去其上具有颗粒的第一基板; 使具有与其结合的印刷材料的颗粒与第二基板接触，使得至少一部分印刷材料结合到第二基板; 并分离衬底，产生印刷衬底。

公开(公告)号：WO2007047761A1

公开(公告)日：2007-04-26

申请号：PCT/US2006/040715

申请日：2006-10-17

Applicant: ARRYX, INC. ,  AKCAKIR, Osman

Inventor： AKCAKIR, Osman

IPC: G01B9/02

CPC classification number: G01Q20/02 ,  G01N21/6456 ,  G01Q30/04 ,  G01Q60/20 ,  G03H2001/0077

Abstract: The present invention utilizes spatially modulated optical force microscopy (SMOFM) with single beam optical force probing capability or with a holographic optical trapping system capable of multi-beam optical force probing coupled to a microscope objective, to generate a probe beam(s) as a force probe to perturb the object that is adhered or resting on a surface, so that deformations of the object may subsequently be quantified. This quantification is performed by imaging a sequence of four phase shifted replicas of the image using a computer-controlled spatial light modulator, and calculating the pixel by pixel optical path-length using existing algorithms. The change in optical path lengths, and consequently the viscoelastic or elastic response elicited, is an indication of damage or disease when the objects are cells. In another embodiment, the optical deformability of the cells may be measured and correlated with measurements of cytoskeletal/structural protein expression.

Abstract translation: 本发明利用具有单光束光学力探测能力的空间调制光学力学显微镜（SMOFM）或利用能够与显微镜物镜耦合的多光束光学力探测的全息光学捕获系统，以产生探针光束 强力探测器扰乱粘附或搁置在表面上的物体，从而可以随后量化物体的变形。 该量化通过使用计算机控制的空间光调制器对图像的四个相移副本的序列进行成像，并使用现有算法计算逐像素光程长度来执行。 光路长度的变化以及因此引起的粘弹性或弹性响应是当物体是细胞时的损伤或疾病的指示。 在另一个实施方案中，可以测量细胞的光学可变形性并与细胞骨架/结构蛋白质表达的测量相关。

公开(公告)号：WO2005089437A3

公开(公告)日：2006-10-26

申请号：PCT/US2005008934

申请日：2005-03-17

Applicant: ARRYX INC ,  PLEWA JOSEPH ,  TANNER EVAN ,  MUETH DAN ,  GRUBER LEWIS ,  BRADLEY KENNETH

Inventor： PLEWA JOSEPH ,  TANNER EVAN ,  MUETH DAN ,  GRUBER LEWIS ,  BRADLEY KENNETH

IPC: H01S1/00 ,  G02B5/18 ,  G02B26/00 ,  G02B27/44 ,  G21K1/00 ,  H01S3/00 ,  H05H3/02 ,  H05H3/04

CPC classification number: B82Y20/00 ,  B82Y10/00 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2225/32

Abstract: A method and apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.

Abstract translation: 一种用于通过用衍射光学元件（DOE）调制激光束而形成的光阱来操纵具有一个或多个特性的微粒（微，纳米和微微）的方法和装置。 选择材料的至少一种特性; 并且产生具有与材料的至少一个所选特征对应的选定波长的激光束。 根据材料的至少一个所选特征计算DOE的值。 光束和DOE被调制以产生具有对应于至少一个所选特征的性质的全息光阱; 陷阱聚焦到光束焦点或选定的光斑尺寸; 并且束聚焦位于颗粒位置附近以将颗粒捕获在其中。

公开(公告)号：WO2003088723A1

公开(公告)日：2003-10-23

申请号：PCT/US2003/010936

申请日：2003-04-10

Applicant: ARRYX, INC. ,  GRIER, David ,  LOPES, Ward

Inventor： GRIER, David ,  LOPES, Ward

IPC: H05H3/04

CPC classification number: G03H1/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2225/32

Abstract: The present invention relates generally to an apparatus and method to generate and control optical traps (1000, 1002) for manipulation of small particles. An upstream modification of an input laser beam (12, 22) provides a beam with a square or other preselected, cross section of intensity which can be used to form optical traps (1000, 1002) with a corresponding cross section of intensity.

Abstract translation: 本发明一般涉及一种用于产生和控制用于操纵小粒子的光阱（1000,1002）的装置和方法。 输入激光束（12,22）的上游修改为光束提供具有正方形或其它预选的强度横截面，其可用于形成具有对应的强度横截面的光学陷阱（1000,1002）。

公开(公告)号：WO2014025895A1

公开(公告)日：2014-02-13

申请号：PCT/US2013/053960

申请日：2013-08-07

Applicant: ARRYX, INC.

Inventor： KNUTSON, Christopher, R. ,  KURELLA, Sridevi ,  DOORNEWEERD, Derek, D. ,  GRANT, Christopher, F. ,  KLINE, Timothy, R.

IPC: G01N33/80 ,  G01N33/68

CPC classification number: G01N33/80 ,  G01N33/6854

Abstract: Methods and devices for evaluating a sample, e.g., a plasma sample, from a subject, for detecting a target red blood cell protein or antibody are disclosed. In one embodiment, optimized antibody screening methods and devices significantly reduce the level of non-specific binding to a surface (e.g., a test surface bound with a red blood cell (rbcm) preparation), thus allowing for more efficient detection and reduced test time. In one embodiment, the optimized antibody screening method includes an immunoglobulin G (IgG) binding moiety that binds selectively and specifically to the plasma IgG present relative to the binding to the lysed rbcm preparation. In another embodiment, an optimized antibody screening method is disclosed whereby non-specific binding caused by lysed red blood cell membrane preparations can be reduced by an agent that specifically cleaves a human IgG in the hinge region. In other embodiments, the invention provides methods and devices for target capturing that include a substantially planar surface, optionally having an optimized angle, for capture. Alternative solid phase geometries for capture are disclosed. Optimized methods for cell deposition are also disclosed. Thus, optimized methods, devices, kits, assays for evaluating a sample are disclosed.

Abstract translation: 公开了用于评估来自受试者的用于检测靶红细胞蛋白或抗体的样品例如血浆样品的方法和装置。 在一个实施方案中，优化的抗体筛选方法和装置显着降低与表面的非特异性结合的水平（例如，与红细胞（rbcm）制剂结合的测试表面），从而允许更有效的检测和减少的测试时间 。 在一个实施方案中，优化的抗体筛选方法包括免疫球蛋白G（IgG）结合部分，所述免疫球蛋白G（IgG）结合部分相对于与裂解的rbcm制剂结合而选择性且特异性地结合存在的血浆IgG。 在另一个实施方案中，公开了优化的抗体筛选方法，其中由裂解的红细胞膜制剂引起的非特异性结合可以通过在铰链区中特异性切割人IgG的试剂降低。 在其他实施例中，本发明提供了用于目标捕获的方法和装置，其包括可选地具有优化角度的基本平坦的表面，用于捕获。 公开了用于捕获的替代固相几何形状。 还公开了细胞沉积的优化方法。 因此，公开了用于评估样品的优化方法，装置，试剂盒测定。

公开(公告)号：WO2011149526A2

公开(公告)日：2011-12-01

申请号：PCT/US2011/000930

申请日：2011-05-25

Applicant: KNUTSON, Christopher ,  AKCAKIR, Osman ,  FU, Haojun ,  ARRYX, INC.

Inventor： KNUTSON, Christopher ,  AKCAKIR, Osman ,  FU, Haojun

IPC: C40B30/06

CPC classification number: G01N33/80 ,  C40B30/04 ,  G01N15/0211 ,  G01N15/1463 ,  G01N21/453 ,  G01N33/5029 ,  G01N33/53 ,  G01N33/54313 ,  G01N2015/0216 ,  G01N2015/1497 ,  G06F19/10

Abstract: The present invention relates to methods and apparatuses for the detection of positional freedom of particles used in biological, biochemical, physical, biophysical, and chemical analyses. In particular, the present invention relates to methods and apparatuses which can detect and characterize a population of particles/cells based upon their detected mobility. In one embodiment consistent with the invention, detection of certain cells is based on differences detected in populations of cells that bind to a substrate and those that exhibit weaker binding forces. Initially, cells are settled on the substrate, and in the presence of gravitational, natural thermodynamic pressure fluctuations, and other random or applied forces, some of the particles may exhibit translational movement. Particle movement is detected, and measurements are computed, according to the methods and apparatuses of the present invention, to determine the binding of specific analytes.

Abstract translation: 本发明涉及用于检测生物，生化，物理，生物物理和化学分析中使用的颗粒的位置自由度的方法和装置。 特别地，本发明涉及可以基于其检测到的移动性来检测和表征粒子/细胞群体的方法和装置。 在与本发明一致的一个实施方案中，某些细胞的检测基于在与底物结合的细胞群体中检测到的差异以及显示较弱结合力的细胞的差异。 最初，细胞沉积在基底上，并且在重力，天然热力学压力波动和其它随机或施加的力的存在下，一些颗粒可能表现出平移运动。 检测到颗粒运动，并且根据本发明的方法和装置计算测量值，以确定特定分析物的结合。

公开(公告)号：WO2006083907A3

公开(公告)日：2006-12-21

申请号：PCT/US2006003462

申请日：2006-02-01

Applicant: ARRYX INC ,  MUETH DANIEL ,  ANDERSON AMY L ,  KNUTSON CHRISTOPHER R ,  PLEWA JOSEPH

Inventor： MUETH DANIEL ,  ANDERSON AMY L ,  KNUTSON CHRISTOPHER R ,  PLEWA JOSEPH

IPC: G01N21/01 ,  G01N33/00

CPC classification number: G01N21/6428 ,  C12M47/04 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/149

Abstract: Apparatus for sorting and orienting sperm cells has a pair or walls (314, 316) in confronting relationship forming a flow chamber (312) having inlet, a downstream outlet, and intermediate detector region (338). The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid (328) containing the cells (330) to be sorted. The first and second streams have respective flow rates relative to third stream, such that the third stream is constricted forming a relatively narrow sample stream, so that the cells (330) are oriented parallel to the walls (314, 316). A detector (340) detect desired cells (330) and a sorter (356) downstream of the detector (340) for sorting the desired cells (330) from the stream.

Abstract translation: 用于分选和定向精子细胞的装置具有面对关系的一对或多个壁（314,316），形成具有入口，下游出口和中间检测器区域（338）的流动室（312）。 入口接收第一和第二间隔开的输入流体流和包含要分选的单元（330）的第三流样品流体（328）。 第一和第二流具有相对于第三流的相应流速，使得第三流收缩形成相对窄的样品流，使得单元（330）平行于壁（314,316）定向。 检测器（340）检测检测器（340）下游的所需单元（330）和分选器（​​356），用于从流中分选所需单元（330）。

公开(公告)号：WO2005060431A9

公开(公告)日：2005-09-22

申请号：PCT/US2004035538

申请日：2004-10-28

Applicant: ARRYX INC ,  PLEWA JOSEPH ,  TANNER EVAN ,  MUETH DAN ,  GRUBER LEWIS ,  BRADLEY KENNETH

Inventor： PLEWA JOSEPH ,  TANNER EVAN ,  MUETH DAN ,  GRUBER LEWIS ,  BRADLEY KENNETH

IPC: G02B5/32 ,  G03H1/08 ,  G03H1/22

CPC classification number: G03H1/22 ,  G02B5/32 ,  G03H1/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/2284

Abstract: A method for trapping particles where a characteristic of a particle is selected, a laser is used to generate a holographic optical trap, and the beams are modulated and focused as desired.

Abstract translation: 一种用于捕获其特征为粒子的颗粒的方法，使用激光来产生全息光阱，并且根据需要调制和聚焦光束。