公开(公告)号：WO2011008553A1

公开(公告)日：2011-01-20

申请号：PCT/US2010/040433

申请日：2010-06-29

Applicant: BATES, Malcolm ,  GOLDSBOROUGH, Andrew ,  LIFE TECHNOLOGIES CORPORATION

Inventor： BATES, Malcolm ,  GOLDSBOROUGH, Andrew

IPC: C12Q1/68

CPC classification number: C12N15/1006 ,  C12N15/1003 ,  C12Q1/6806 ,  Y02A50/53 ,  Y02A50/54 ,  Y02A50/60 ,  C12Q2563/137 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: This disclosure relates to reagents, compositions, methods for stabilising RNA in an RNA-containing sample by contacting the sample with guanidine and a metal ion to form a stabilised RNA-containing composition in which the metal ion is present at a concentration which is no more than 20mM, and the metal ion is derived from a metal other than from a Group 1 or Group 2 metal.

Abstract translation: 本公开涉及用于通过使样品与胍和金属离子接触来稳定RNA的RNA的试剂，组合物，方法，以形成稳定的含RNA组合物，其中金属离子以不再多的浓度存在 超过20mM，金属离子衍生自不同于第1族或第2族金属的金属。

公开(公告)号：WO2021205042A1

公开(公告)日：2021-10-14

申请号：PCT/EP2021/059465

申请日：2021-04-12

Applicant: GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert ,  RNASSIST LIMITED

Inventor： GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert

IPC: C12N7/06 ,  C11D3/48

Abstract: Provided is the use of a composition to inactive a virus. The composition comprises a quaternary ammonium compound and a halogenated organic compound. Also provided are a composition and kit for inactivating a virus, and a method for detecting an analyte in a sample.

公开(公告)号：WO2014131906A1

公开(公告)日：2014-09-04

申请号：PCT/EP2014/054009

申请日：2014-02-28

Applicant: GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert

Inventor： GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert

IPC: A01N1/02 ,  B01L3/00

CPC classification number: G01N1/30 ,  A01N1/0231 ,  G01N2001/307

Abstract: It is possible to stabilise biomolecules such as RNA, DNA and proteins in biological samples such as cells, tissues, biopsies and blood using deep eutectic solvents (DES). DES mixtures can be used to fix and preserve cell morphology in biological samples such as tissue blocks, cancer biopsies and whole blood. This invention describes methods to stabilise and preserve biomolecules, whole cells, tissues, blood and biological samples using DES mixtures.

Abstract translation: 使用深层共晶溶剂（DES）可以在生物样品如细胞，组织，活组织检查和血液中稳定生物分子，如RNA，DNA和蛋白质。 DES混合物可用于固定和保存生物样品中的细胞形态，如组织块，癌症活检和全血。 本发明描述了使用DES混合物稳定和保存生物分子，全细胞，组织，血液和生物样品的方法。

公开(公告)号：WO2020020913A1

公开(公告)日：2020-01-30

申请号：PCT/EP2019/069846

申请日：2019-07-23

Applicant: GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert

Inventor： GOLDSBOROUGH, Andrew Simon ,  BATES, Malcolm Robert

IPC: G01N1/28 ,  B01L3/00 ,  C12M3/02 ,  C12M1/06

Abstract: A method of processing a biological sample to obtain single cells and/or groups of cells comprises: fixing cells of the biological sample using a fixative; contacting the biological sample with a dissociation medium; dissociating the biological sample in the presence of the dissociation medium to obtain the single cells and/or groups of cells; wherein the fixative comprises a deep eutectic solvent. Fixing cells of the biological sample using a deep eutectic solvent facilitates dissociation of the biological sample into single cells, while at the same time inhibiting the degradation of biomolecules such as DNA and RNA in the cells. Also provided is an apparatus useful in the method, deep eutectic solvents and uses thereof, and a method of extracting cell nuclei.