公开(公告)号：WO2005090396A1

公开(公告)日：2005-09-29

申请号：PCT/NL2005/000216

申请日：2005-03-22

申请人： WAGENINGEN CENTRE FOR FOOD SCIENCES ,  PRETZER, Gabriele ,  SNEL, Johannes ,  BRON, Peter, Allard ,  DE VOS, Willem, Meindert ,  KLEEREBEZEM, Michiel

发明人： PRETZER, Gabriele ,  SNEL, Johannes ,  BRON, Peter, Allard ,  DE VOS, Willem, Meindert ,  KLEEREBEZEM, Michiel

IPC分类号： C07K14/335

CPC分类号： C07K14/335 ,  A61K38/00

摘要： The present invention relates to novel mannose-specific adhesins, and variants thereof, as well as nucleic acid sequences encoding these. The invention also relates to host cells expressing nucleic acids encoding the mannose-specific adhesins or variants thereof, as well as to pharmaceutical or nutraceutical compositions comprising the mannose-specific adhesins and/or host cells expressing the adhesins, and their use in treating or preventing bacterial infection. Further, screening methods suitable for identifying bacteria strains with probiotic properties are provided.

摘要翻译： 本发明涉及新型甘露糖特异性粘附素及其变体，以及编码这些粘附素的核酸序列。 本发明还涉及表达编码甘露糖特异性粘附素或其变体的核酸的宿主细胞，以及包含表达粘附素的甘露糖特异性粘附素和/或宿主细胞的药物或营养组合物及其在治疗或预防中的用途 细菌感染。 此外，提供了适用于鉴定具有益生菌特性的细菌菌株的筛选方法。

公开(公告)号：WO2012150861A1

公开(公告)日：2012-11-08

申请号：PCT/NL2012/050284

申请日：2012-04-26

申请人： STICHTING TOP INSTITUTE FOOD AND NUTRITION ,  VAN BOKHORST-VAN DE VEEN, Hermien ,  LEE,, I-Chiao ,  WELS, Michiel Wouter Wilhelmus ,  DE VOS, Paulus ,  BRON, Peter Allard ,  BONGERS, Roger Samuel ,  WIERSMA, Anne ,  KLEEREBEZEM, Michiel

发明人： VAN BOKHORST-VAN DE VEEN, Hermien ,  LEE,, I-Chiao ,  WELS, Michiel Wouter Wilhelmus ,  DE VOS, Paulus ,  BRON, Peter Allard ,  BONGERS, Roger Samuel ,  WIERSMA, Anne ,  KLEEREBEZEM, Michiel

IPC分类号： C12N1/21 ,  C07K14/335 ,  A23L1/03 ,  A23L1/30

CPC分类号： A23L1/3014 ,  A23L29/065 ,  A23L33/135 ,  C07K14/335 ,  C12N1/20

摘要： The present invention relates to a method for screening for a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract. The present invention further relates to a method for screening for culture conditions that provide a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract. The present invention further relates to a method for modulating the expression of certain polynucleotides. The present invention further relates to a method for the preparation of a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract. The present invention further relates to a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract. The present invention further relates to a method for the preparation of a food composition. The present invention further relates to a food composition. The present invention further relates to the use of certain polynucleotides in the screening for a bacterium exhibiting enhanced survival properties in the gastro- intestinal tract and/or the screening for culture conditions that provide a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract and/or for the control of culture conditions providing a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract. The present invention further relates to a method for controlling culture conditions providing a bacterium exhibiting enhanced survival properties in the gastro-intestinal tract.

摘要翻译： 本发明涉及筛选在胃肠道中表现出增强的存活性的细菌的方法。 本发明还涉及一种用于筛选提供在胃肠道中表现出增强的存活性的细菌的培养条件的方法。 本发明还涉及调节某些多核苷酸表达的方法。 本发明还涉及一种制备在胃肠道中表现出增强的存活性的细菌的方法。 本发明还涉及在胃肠道中表现出增强的存活性的细菌。 本发明还涉及一种食品组合物的制备方法。 本发明还涉及食品组合物。 本发明还涉及某些多核苷酸在筛选在胃肠道中表现出增强的存活性的细菌和/或筛选提供在胃肠道中表现出增强的存活性的细菌的培养条件下的某些多核苷酸的用途，以及 或用于控制提供在胃肠道中表现出增强的存活性的细菌的培养条件。 本发明还涉及一种控制培养条件的方法，所述培养条件提供在胃肠道中表现出增强的存活性的细菌。

公开(公告)号：WO2005040387A1

公开(公告)日：2005-05-06

申请号：PCT/NL2003/000733

申请日：2003-10-28

申请人： FRIESLAND BRANDS B.V. ,  BRON, Peter, Allard ,  DE VOS, Willem, Meindert ,  KLEEREBEZEM, Michiel

发明人： BRON, Peter, Allard ,  DE VOS, Willem, Meindert ,  KLEEREBEZEM, Michiel

IPC分类号： C12N15/74

CPC分类号： A61K48/0058 ,  A61K48/00 ,  A61K48/0075 ,  A61K2035/11 ,  C12N15/746

摘要： The present invention relates to the site-specific production of biologically active substances by recombinant micro-organisms in body cavities of a human or animal subject. The invention provides transcription regulatory sequences, which are specifically induced in the gastrointestinal tract (GI-tract) of subjects and methods for their use for in vivo site-specific expression of nucleic acid sequences encoding biologically active substances.

摘要翻译： 本发明涉及通过重组微生物在人或动物受试者的体腔中的生物活性物质的位点特异性生产。 本发明提供在受试者的胃肠道（GI道）中特异性诱导的转录调节序列及其用于体内位点特异性表达编码生物活性物质的核酸序列的方法。

公开(公告)号：WO2011059332A3

公开(公告)日：2011-07-07

申请号：PCT/NL2010050760

申请日：2010-11-16

申请人： STICHTING TOP INST FOOD AND NUTRITION ,  VAN HEMERT SASKIA ,  MEIJERINK MARJOLEIN ,  BRON PETER ALLARD ,  MARCO MARIA LOUISE ,  KLEEREBEZEM MICHIEL ,  DE VOS PAULUS ,  WELS MICHIEL WOUTER WILHELMUS ,  WELLS JEREMY MARK

发明人： VAN HEMERT SASKIA ,  MEIJERINK MARJOLEIN ,  BRON PETER ALLARD ,  MARCO MARIA LOUISE ,  KLEEREBEZEM MICHIEL ,  DE VOS PAULUS ,  WELS MICHIEL WOUTER WILHELMUS ,  WELLS JEREMY MARK

IPC分类号： C12N15/74

CPC分类号： C12N1/36 ,  A23V2002/00 ,  A61K35/74 ,  C07K14/335 ,  C12N1/20 ,  C12N9/12 ,  A23V2200/324

摘要： The invention relates to a method for preparing a bacterium capable of immunomodulation, having either anti-inflammatory or pro -inflammatory capacities, by incorporating or knocking-out one or more genes. The invention also relates to modulating expression of these one or more genes by adapting fermentation conditions. The invention further provides for a method for identifying a bacterium capable of immunomodulation, a recombinant bacterium obtainable by any of such methods, compositions comprising such recombinant bacterium, and their use as a medicament. Also, the use of protein and nucleic acid sequences as a medicament is described.

摘要翻译： 本发明涉及通过掺入或敲除一种或多种基因来制备能够进行免疫调节的具有抗炎或促炎能力的细菌的方法。 本发明还涉及通过调节发酵条件来调节这些一种或多种基因的表达。 本发明进一步提供了用于鉴定能够免疫调节的细菌的方法，可通过任何这些方法获得的重组细菌，包含这种重组细菌的组合物及其作为药物的用途。 另外，描述了使用蛋白质和核酸序列作为药物。

公开(公告)号：WO2011059332A2

公开(公告)日：2011-05-19

申请号：PCT/NL2010/050760

申请日：2010-11-16

申请人： STICHTING TOP INSTITUTE FOOD AND NUTRITION ,  VAN HEMERT, Saskia ,  MEIJERINK, Marjolein ,  BRON, Peter Allard ,  MARCO, Maria Louise ,  KLEEREBEZEM, Michiel ,  DE VOS, Paulus ,  WELS, Michiel Wouter Wilhelmus ,  WELLS, Jeremy Mark

发明人： VAN HEMERT, Saskia ,  MEIJERINK, Marjolein ,  BRON, Peter Allard ,  MARCO, Maria Louise ,  KLEEREBEZEM, Michiel ,  DE VOS, Paulus ,  WELS, Michiel Wouter Wilhelmus ,  WELLS, Jeremy Mark

IPC分类号： C12N15/74

CPC分类号： C12N1/36 ,  A23V2002/00 ,  A61K35/74 ,  C07K14/335 ,  C12N1/20 ,  C12N9/12 ,  A23V2200/324

摘要： The invention relates to a method for preparing a bacterium capable of immunomodulation, having either anti-inflammatory or pro -inflammatory capacities, by incorporating or knocking-out one or more genes. The invention also relates to modulating expression of these one or more genes by adapting fermentation conditions. The invention further provides for a method for identifying a bacterium capable of immunomodulation, a recombinant bacterium obtainable by any of such methods, compositions comprising such recombinant bacterium, and their use as a medicament. Also, the use of protein and nucleic acid sequences as a medicament is described.

摘要翻译： 本发明涉及通过掺入或敲除一个或多个基因来制备具有抗炎或促炎症能力的免疫调节细菌的方法。 本发明还涉及通过调整发酵条件来调节这些一个或多个基因的表达。 本发明进一步提供了用于鉴定能够免疫调节的细菌的方法，通过任何这样的方法可获得的重组细菌，包含所述重组细菌的组合物，以及它们作为药物的用途。 另外，描述了蛋白质和核酸序列作为药物的用途。