公开(公告)号：WO2007025248B1

公开(公告)日：2007-10-11

申请号：PCT/US2006033498

申请日：2006-08-24

Applicant: WYETH CORP ,  MOSYAK LIDIA ,  RUSH III THOMAS SALTMARSH ,  ZHONG XIAOTIAN ,  MCDONAGH THOMAS E ,  GEORGIADIS KATY E ,  SUM PHAIK-ENG ,  LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  KUMAR RAVINDRA ,  HEBERT TRACY ,  OLLAND STEPHANE HUBERT ,  MACKIE STEWART ANDREWS

Inventor： MOSYAK LIDIA ,  RUSH III THOMAS SALTMARSH ,  ZHONG XIAOTIAN ,  MCDONAGH THOMAS E ,  GEORGIADIS KATY E ,  SUM PHAIK-ENG ,  LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  KUMAR RAVINDRA ,  HEBERT TRACY ,  OLLAND STEPHANE HUBERT ,  MACKIE STEWART ANDREWS

IPC: C12N9/64 ,  C30B7/00 ,  G01N23/20 ,  G06F19/00

CPC classification number: C12N9/6421 ,  C07K2299/00 ,  C30B7/00

Abstract: This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

公开(公告)号：WO2007025248A2

公开(公告)日：2007-03-01

申请号：PCT/US2006033498

申请日：2006-08-24

Applicant: WYETH CORP ,  MOSYAK LIDIA ,  RUSH III THOMAS SALTMARSH ,  ZHONG XIAOTIAN ,  MCDONAGH THOMAS E ,  GEORGIADIS KATY E ,  SUM PHAIK-ENG ,  LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  KUMAR RAVINDRA ,  HEBERT TRACY ,  OLLAND STEPHANE HUBERT ,  MACKIE STEWART ANDREWS

Inventor： MOSYAK LIDIA ,  RUSH III THOMAS SALTMARSH ,  ZHONG XIAOTIAN ,  MCDONAGH THOMAS E ,  GEORGIADIS KATY E ,  SUM PHAIK-ENG ,  LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  KUMAR RAVINDRA ,  HEBERT TRACY ,  OLLAND STEPHANE HUBERT ,  MACKIE STEWART ANDREWS

IPC: C07K14/43

CPC classification number: C12N9/6421 ,  C07K2299/00 ,  C30B7/00

Abstract: This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

Abstract translation: 本发明涉及聚集蛋白聚糖酶多肽和聚集蛋白聚糖酶多肽/配体复合物，聚集蛋白聚糖酶和聚集蛋白聚糖酶多肽/配体复合物的晶体以及相关方法和软件系统。

公开(公告)号：WO2004011637A2

公开(公告)日：2004-02-05

申请号：PCT/US2003/023484

申请日：2003-07-29

Applicant: WYETH ,  CORCORAN, Christopher, John ,  FLANNERY, Carl, R. ,  ZENG, Weilan ,  RACIE, Lisa, A. ,  MCDONAGH, Thomas ,  FREEMAN, Bethany, A. ,  GEORGIADIS, Katy, E. ,  LAVALLIE, Edward, R.

Inventor： CORCORAN, Christopher, John ,  FLANNERY, Carl, R. ,  ZENG, Weilan ,  RACIE, Lisa, A. ,  MCDONAGH, Thomas ,  FREEMAN, Bethany, A. ,  GEORGIADIS, Katy, E. ,  LAVALLIE, Edward, R.

IPC: C12N9/00

CPC classification number: C12N9/6489 ,  A61K2039/505

Abstract: The present invention relates to modified ADAMTS4 proteins having improved stability comparing to the corresponding native, unmodified proteins. The modified ADAMTS4 proteins can be expressed and isolated in large quantities, thus allowing further characterization of the proteins, such as crystallographic and enzyme kinetic studies. The purified, stable proteins would also facilitate the production of antiADAMTS antibodies and the development of inhibitors to ADAMTS enzymes.

Abstract translation: 本发明涉及与相应的天然未修饰蛋白质相比具有改善的稳定性的修饰的ADAMTS4蛋白质。 修饰后的ADAMTS4蛋白可以大量表达和分离，从而可以进一步表征蛋白质，如结晶学和酶动力学研究。 纯化的稳定蛋白质也将促进抗ADAMTS抗体的产生和ADAMTS酶抑制剂的开发。

公开(公告)号：WO2005103287A1

公开(公告)日：2005-11-03

申请号：PCT/US2005/012997

申请日：2005-04-18

Applicant: WYETH ,  LAVALLIE, Edward ,  COLLINS-RACIE, Lisa, A. ,  CORCORAN, Christopher, John ,  TWINE, Natalie, C. ,  AGOSTINO, Michael, J.

Inventor： LAVALLIE, Edward ,  COLLINS-RACIE, Lisa, A. ,  CORCORAN, Christopher, John ,  TWINE, Natalie, C. ,  AGOSTINO, Michael, J.

IPC: C12Q1/37

CPC classification number: C12N9/6489

Abstract: The invention provides truncated biologically active ADAMTS polypeptides, particularly those with hyalectenase activity, and more particularly those with aggrecanase activity, that exhibit greater stability and homogeneity and higher expression yields than their full-length counterparts. The invention also provides nucleic acid molecules encoding such truncated biologically active ADAMTS polypeptides and methods for producing the truncated biologically active ADAMTS polypeptides. In addition, the invention provides methods for identifying compounds capable of modulating biologically active ADAMTS polypeptides, particularly those compounds that inhibit aggrecanase activity.

Abstract translation: 本发明提供截短的生物活性ADAMTS多肽，特别是那些具有透明质酸酶活性的多肽，更具体地，具有聚集蛋白聚糖蛋白聚糖酶活性的多肽，其表现出比其全长对应物更大的稳定性和均一性和更高的表达产率。 本发明还提供了编码这种截短的生物活性ADAMTS多肽的核酸分子和用于产生截短的生物活性ADAMTS多肽的方法。 此外，本发明提供了鉴定能够调节生物活性ADAMTS多肽，特别是那些抑制聚蛋白聚糖蛋白酶活性的化合物的化合物的方法。

公开(公告)号：WO2007025248A3

公开(公告)日：2007-03-01

申请号：PCT/US2006/033498

申请日：2006-08-24

Applicant: WYETH ,  MOSYAK, Lidia ,  RUSH III, Thomas Saltmarsh ,  ZHONG, Xiaotian ,  MCDONAGH, Thomas E. ,  GEORGIADIS, Katy E. ,  SUM, Phaik-Eng ,  LAVALLIE, Edward R. ,  COLLINS-RACIE, Lisa A. ,  CORCORAN, Christopher John ,  KUMAR, Ravindra ,  HEBERT, Tracy ,  OLLAND, Stephane Hubert ,  MACKIE, Stewart Andrews

Inventor： MOSYAK, Lidia ,  RUSH III, Thomas Saltmarsh ,  ZHONG, Xiaotian ,  MCDONAGH, Thomas E. ,  GEORGIADIS, Katy E. ,  SUM, Phaik-Eng ,  LAVALLIE, Edward R. ,  COLLINS-RACIE, Lisa A. ,  CORCORAN, Christopher John ,  KUMAR, Ravindra ,  HEBERT, Tracy ,  OLLAND, Stephane Hubert ,  MACKIE, Stewart Andrews

IPC: C12N9/64 ,  G06F19/00 ,  G01N23/20 ,  C30B7/00

Abstract: This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

公开(公告)号：WO2005116197A2

公开(公告)日：2005-12-08

申请号：PCT/US2005012539

申请日：2005-04-15

Applicant: WYETH CORP ,  LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  AGOSTINO MICHAEL J ,  FREEMAN BETHANY A ,  ARAI MAYA ,  FLANNERY CARL R ,  JIN MACY X

Inventor： LAVALLIE EDWARD R ,  COLLINS-RACIE LISA A ,  CORCORAN CHRISTOPHER JOHN ,  AGOSTINO MICHAEL J ,  FREEMAN BETHANY A ,  ARAI MAYA ,  FLANNERY CARL R ,  JIN MACY X

IPC: A61K38/48 ,  C12N9/00 ,  C12N9/64 ,  C12Q1/37

CPC classification number: A61K38/4886 ,  C12N9/6489 ,  C12Q1/37 ,  G01N2333/96486 ,  G01N2400/00 ,  G01N2500/00

Abstract: The present invention features methods of using ADAMTS-8 proteins or their functional derivatives to cleave aggrecan or other proteoglycan molecules. The present invention also features methods for identifying ADAMTS-8 modulators that are capable of inhibiting or enhancing ADAMTS-8 proteolytic activities. In addition, the present invention features pharmaceutical compositions comprising ADAMTS-8 proteins or their derivatives or modulators. These pharmaceutical compositions can be used to treat diseases that are characterized by deficiencies or abnormalities in proteoglycan cleavage or metabolism.

Abstract translation: 本发明的特征在于使用ADAMTS-8蛋白或其功能衍生物切割聚蛋白聚糖蛋白聚糖或其他蛋白聚糖分子的方法。 本发明还涉及用于鉴定能够抑制或增强ADAMTS-8蛋白水解活性的ADAMTS-8调节剂的方法。 此外，本发明的特征在于包含ADAMTS-8蛋白质或其衍生物或调节剂的药物组合物。 这些药物组合物可用于治疗以蛋白多糖切割或代谢为特征的缺陷或异常的疾病。

公开(公告)号：WO2004011637A3

公开(公告)日：2004-04-08

申请号：PCT/US0323484

申请日：2003-07-29

Applicant: WYETH CORP ,  CORCORAN CHRISTOPHER JOHN ,  FLANNERY CARL R ,  ZENG WEILAN ,  RACIE LISA A ,  MCDONAGH THOMAS ,  FREEMAN BETHANY A ,  GEORGIADIS KATY E ,  LAVALLIE EDWARD R

Inventor： CORCORAN CHRISTOPHER JOHN ,  FLANNERY CARL R ,  ZENG WEILAN ,  RACIE LISA A ,  MCDONAGH THOMAS ,  FREEMAN BETHANY A ,  GEORGIADIS KATY E ,  LAVALLIE EDWARD R

IPC: G01N33/50 ,  A61K39/395 ,  A61K45/00 ,  A61P19/00 ,  A61P19/02 ,  A61P43/00 ,  C12N9/64 ,  C12N15/09 ,  C12Q1/37 ,  G01N33/15

CPC classification number: C12N9/6489 ,  A61K2039/505

Abstract: The present invention relates to modified ADAMTS4 proteins having improved stability comparing to the corresponding native, unmodified proteins. The modified ADAMTS4 proteins can be expressed and isolated in large quantities, thus allowing further characterization of the proteins, such as crystallographic and enzyme kinetic studies. The purified, stable proteins would also facilitate the production of antiADAMTS antibodies and the development of inhibitors to ADAMTS enzymes.

Abstract translation: 本发明涉及与相应的天然未修饰蛋白相比具有改进的稳定性的修饰的ADAMTS4蛋白。 修饰的ADAMTS4蛋白可以大量表达和分离，从而允许进一步表征蛋白质，如晶体学和酶动力学研究。 纯化的稳定蛋白质也有助于抗ADAMTS抗体的产生和ADAMTS酶抑制剂的开发。

公开(公告)号：WO2005016130A2

公开(公告)日：2005-02-24

申请号：PCT/US2004/026508

申请日：2004-08-13

Applicant: WYETH ,  FLANNERY, Carl Ralph ,  CORCORAN, Christopher John ,  FREEMAN, Bethany Annis ,  RACIE, Lisa Anne

Inventor： FLANNERY, Carl Ralph ,  CORCORAN, Christopher John ,  FREEMAN, Bethany Annis ,  RACIE, Lisa Anne

IPC: A61B

CPC classification number: A61K38/1709 ,  A61K38/00 ,  A61K38/18 ,  A61K38/19 ,  A61K45/06 ,  C07K14/47 ,  C07K14/475 ,  C07K16/18

Abstract: Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.

Abstract translation: 重组润滑剂分子及其用途。 提供新的重组润滑剂分子及其作为润滑剂，抗粘连剂和/或关节内补充剂用于例如滑膜关节，半月板，腱，腹膜，心包和胸膜的用途。

公开(公告)号：WO2009058346A1

公开(公告)日：2009-05-07

申请号：PCT/US2008/012338

申请日：2008-10-31

Applicant: WYETH ,  LAVALLIE, Edward, Roland ,  COLLINS-RACIE, Lisa Anne ,  CORCORAN, Christopher, John ,  TCHISTIAKOVA, Lioudmila, Gennadievna ,  NOWAK, John, Adam ,  KARIM, Riyez ,  TAN, Xiang-Yang ,  MARQUETTE, Kimberly, Ann ,  VELDMAN, Geertruida, Machteld

Inventor： LAVALLIE, Edward, Roland ,  COLLINS-RACIE, Lisa Anne ,  CORCORAN, Christopher, John ,  TCHISTIAKOVA, Lioudmila, Gennadievna ,  NOWAK, John, Adam ,  KARIM, Riyez ,  TAN, Xiang-Yang ,  MARQUETTE, Kimberly, Ann ,  VELDMAN, Geertruida, Machteld

IPC: C07K16/22

CPC classification number: C07K16/22 ,  A61K39/395 ,  A61K2039/505 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94

Abstract: The disclosure provides novel molecules related to growth and differentiation factor 8 (GDF8), in particular epitopes specific to GDF8 and other specific antagonists of GDF8 in particular anti GDF8 antibodies or antigen binding protein or fragment thereof that may inhibit GDF8 activity and signal in vitro and/or in vivo. The disclosure also provides for an immunoassay used to detect and quantitate GDF8. The disclosure also provides methods for diagnosing, preventing, ameliorating, and treating GDF8 associated disorders, e.g., degenerative orders of muscle, bone, and insulin metabolism. Finally, the disclosure provides pharmaceuticals for the treatment of such disorders by using the antibodies, polypeptides, polynucleotides, and vectors of the invention.

Abstract translation: 本公开提供了与生长和分化因子8（GDF8）有关的新分子，特别是GDF8特异性表位和GDF8特别是抗GDF8抗体或抗原结合蛋白或其片段的其他特异性拮抗剂，其可抑制体外GDF8活性和信号， /或体内。 本公开还提供用于检测和定量GDF8的免疫测定。 本公开还提供了用于诊断，预防，改善和治疗GDF8相关疾病例如肌肉，骨骼和胰岛素代谢的退行性顺序的方法。 最后，本公开提供了通过使用本发明的抗体，多肽，多核苷酸和载体来治疗这种疾病的药物。

公开(公告)号：WO2005016130A3

公开(公告)日：2006-02-02

申请号：PCT/US2004026508

申请日：2004-08-13

Applicant: WYETH CORP ,  FLANNERY CARL RALPH ,  CORCORAN CHRISTOPHER JOHN ,  FREEMAN BETHANY ANNIS ,  RACIE LISA ANNE

Inventor： FLANNERY CARL RALPH ,  CORCORAN CHRISTOPHER JOHN ,  FREEMAN BETHANY ANNIS ,  RACIE LISA ANNE

IPC: A61B20060101 ,  C07K14/475 ,  A61K38/17 ,  A61K31/728 ,  C07K14/435

CPC classification number: A61K38/1709 ,  A61K38/00 ,  A61K38/18 ,  A61K38/19 ,  A61K45/06 ,  C07K14/47 ,  C07K14/475 ,  C07K16/18

Abstract: Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.

Abstract translation: 重组润滑剂分子及其用途。 提供新的重组润滑剂分子及其用作润滑剂，抗粘合剂和/或关节内补充剂用于例如滑膜关节，半月板，腱，腹膜，心包和胸膜。