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18 条记录 (耗时 0.014 秒)

    REGULATION OF CELLULAR FUNCTIONS BY ECTOPIC EXPRESSION OF NON-ENDOGENOUS CELL SIGNALLING RECEPTORS
    3.
    发明申请
    REGULATION OF CELLULAR FUNCTIONS BY ECTOPIC EXPRESSION OF NON-ENDOGENOUS CELL SIGNALLING RECEPTORS 审中-公开
    通过非内源细胞信号受体的表达表达调节细胞功能

    公开(公告)号：WO1997007233A1

    公开(公告)日：1997-02-27

    申请号：PCT/US1996013077

    申请日：1996-08-12

    申请人： CORNELL RESEARCH FOUNDATION, INC. ,  GERSHENGORN, Marvin, C. ,  FALCK-PEDERSEN, Erik, S. ,  CRYSTAL, Ronald, G.

    发明人： CORNELL RESEARCH FOUNDATION, INC.

    IPC分类号： C12P21/06

    CPC分类号： C07K14/72 ,  A61K38/00

    摘要： The present invention is directed to an in vivo cell transformed with DNA encoding a cell signalling receptor not endogenous to the cell. The cell signalling receptor is capable of activating a signal transduction pathway endogenous to the cell, and the cell signalling receptor can be controllably activated thereby controllably activating the signal transduction pathway so as to regulate a cell function controlled by the signal transduction pathway. The invention also provides a method of ectopically expressing a non-endogenous receptor in a cell, and a method of regulating a cell function in vivo. The method of regulating a cell function comprises transforming a cell with DNA encoding a cell signalling receptor not endogenous to the cell, as above, and controllably exposing the cell to an extracellular molecule capable of activating the foreign cell signalling receptor. Activation of the cell signalling receptor activates the endogenous signal transduction pathway so as to regulate a cell function controlled by the endogenous signal transduction pathway.

    摘要翻译： 本发明涉及用编码细胞内源性的细胞信号传导受体的DNA转化的体内细胞。 细胞信号传导受体能够激活细胞内源性的信号转导途径，并且细胞信号传导受体可被可控地激活，从而可控制地激活信号转导途径，从而调节由信号转导途径控制的细胞功能。 本发明还提供了在细胞中异位表达非内源受体的方法，以及调节体内细胞功能的方法。 调节细胞功能的方法包括如上所述用编码细胞内源性的细胞信号传导受体的DNA转化细胞，并可控地将细胞暴露于能够激活外源细胞信号传导受体的细胞外分子。 细胞信号传导受体的激活激活内源性信号转导途径，以调节由内源信号转导途径控制的细胞功能。

    ALTERNATIVELY SPLICED NUCLEIC ACID MOLECULES
    5.
    发明申请
    ALTERNATIVELY SPLICED NUCLEIC ACID MOLECULES 审中-公开
    交替分离的核酸分子

    公开(公告)号：WO2003091271A1

    公开(公告)日：2003-11-06

    申请号：PCT/US2003/012709

    申请日：2003-04-24

    申请人： CORNELL RESEARCH FOUNDATION, INC. ,  CRYSTAL, Ronald, G. ,  HACKETT, Neil, R.

    发明人： CRYSTAL, Ronald, G. ,  HACKETT, Neil, R.

    IPC分类号： C07H21/04

    CPC分类号： C07K14/52

    摘要： The invention provides an isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence of at least 1877 contiguous nucleotides of (a) SEQ ID NO: 1 or (b) a nucleotide sequence encoding an angiogenic factor, wherein the nucleotide sequence comprises cDNA comprising exons 1-5 of a vascular endothelial growth factor (VEGF) joined to genomic DNA comprising introns 5, 6, and 7 and exons 6, 7, and 8 of VEGF and further comprising a mutation in one or more of the splice donor, branch point, and splice acceptor regions contained in an exon selected from the group consisting of exons 5, 6, 7, and 8, wherein the mutation promotes the production of one isoform of VEGF as compared to another isoform of VEGF. Expression constructs, compositions, and cells comprising such a nucleic acid molecule also are provided by the invention.

    摘要翻译： 本发明提供了分离或纯化的核酸分子，其基本上由（a）SEQ ID NO：1的至少1877个连续核苷酸的核苷酸序列组成，或（b）编码血管生成因子的核苷酸序列，其中所述核苷酸序列包含 血管内皮生长因子（VEGF）的外显子1-5连接到包含内含子5,6和7以及VEGF的外显子6,7和8的基因组DNA上，并且还包含一个或多个剪接供体，分支 包含在选自外显子5,6,7和8的外显子中的位点和剪接受体区，其中与VEGF的另一同工型相比，突变促进VEGF的一种同工型的产生。 包含这种核酸分子的表达构建体，组合物和细胞也由本发明提供。