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公开(公告)号：WO2011035422A1
公开(公告)日：2011-03-31
申请号：PCT/CA2010/001488
申请日：2010-09-21
申请人： MEDICAGO INC. , VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant- derived VLPs are recovered from the filtered fraction.
摘要翻译： 提供了植物来源的病毒样颗粒（VLPs）的制备方法。 该方法可以包括获得植物或包含原生质体定位的VLP的植物物质，从植物或植物物质产生原生质体/原生质体级分和原生质体级分，以及回收载脂蛋白级分。 载脂蛋白级分包含植物来源的VLP。 或者，VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得，以产生消化部分。 将消化的馏分过滤以产生过滤的馏分，并从过滤的馏分中回收来自植物的VLP。
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2.发明申请CHIMERIC INFLUENZA VIRUS-LIKE PARTICLES COMPRISING HEMAGGLUTININ 审中-公开含有HEMAGGLUTININ的CHIMERIC INFLUENZA病毒颗粒
公开(公告)号：WO2010148511A8
公开(公告)日：2011-03-10
申请号：PCT/CA2010000983
申请日：2010-06-25
申请人： MEDICAGO INC , COUTURE MANON , DARGIS MICHELE , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE , D AOUST MARC-ANDRE
发明人： COUTURE MANON , DARGIS MICHELE , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE , D AOUST MARC-ANDRE
IPC分类号： C07K14/11 , A01H5/00 , A61K39/145 , A61P31/16 , C07K19/00 , C12N7/00 , C12N7/01 , C12N15/44 , C12N15/82
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译： 提供了一种在植物或植物的一部分内合成嵌合型流感病毒样颗粒（VLP）的方法。 该方法包括在植物或植物的一部分中表达嵌合流感HA。 本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。 本发明还涉及编码嵌合流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。
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公开(公告)号：WO2011035423A1
公开(公告)日：2011-03-31
申请号：PCT/CA2010/001489
申请日：2010-09-21
申请人： MEDICAGO INC. , VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins,. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
摘要翻译： 提供了制备植物衍生蛋白或超基质蛋白的方法。 该方法可以包括获得植物或包含质外体蛋白质定位蛋白质的植物物质或超基质蛋白，从植物或植物物质产生原生质体/原生质体级分和质外体级分，并回收载脂蛋白级分。 载脂蛋白级分包含植物来源的蛋白质或超基因蛋白质。 或者，可以通过使用细胞壁降解酶组合物消化植物物质以产生消化部分，从包含植物衍生的蛋白质或超基质蛋白质的植物或植物物质获得蛋白质或超基质蛋白质。 将消化的馏分过滤以产生过滤的馏分，从过滤的馏分中回收植物来源的蛋白质或超结构蛋白质。
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4.发明申请CHIMERIC INFLUENZA VIRUS-LIKE PARTICLES COMPRISING HEMAGGLUTININ 审中-公开含有HEMAGGLUTININ的CHIMERIC INFLUENZA病毒颗粒
公开(公告)号：WO2010148511A1
公开(公告)日：2010-12-29
申请号：PCT/CA2010/000983
申请日：2010-06-25
申请人： MEDICAGO, INC. , COUTURE, Manon , DARGIS, Michele , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , D'AOUST, Marc-Andre
发明人： COUTURE, Manon , DARGIS, Michele , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , D'AOUST, Marc-Andre
IPC分类号： C07K14/11 , A01H5/00 , A61K39/145 , A61P31/16 , C12N15/44 , C12N15/82 , C12N7/00 , C12N7/01 , C07K19/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译： 提供了在植物或植物的一部分内合成嵌合型流感病毒样颗粒（VLP）的方法。 该方法包括在植物或植物的一部分中表达嵌合流感HA。 本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。 本发明还涉及编码嵌合流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。
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5.发明申请RECOMBINANT INFLUENZA VIRUS-LIKE PARTICLES (VLPS) PRODUCED IN TRANSGENIC PLANTS EXPRESSING HEMAGGLUTININ 审中-公开转基因植物中生产的重组流感病毒样芽孢杆菌（VLPS）表达HEMAGGLUTININ
公开(公告)号：WO2009076778A8
公开(公告)日：2009-08-27
申请号：PCT/CA2009000032
申请日：2009-01-12
申请人： MEDICAGO INC , D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
发明人： D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
IPC分类号： C12N15/82 , A01H5/00 , A23L33/00 , A61K35/76 , A61K39/145 , A61P31/16 , A61P37/04 , C07K14/11 , C12N7/00 , C12N7/01 , C12N15/44
CPC分类号： C12N15/8258 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/55588 , A61K2039/58 , C07K14/005 , C07K2319/02 , C07K2319/03 , C12N7/00 , C12N9/90 , C12N15/8257 , C12N2760/16022 , C12N2760/16122 , C12N2760/16123 , C12N2760/16133 , C12N2760/16134 , C12N2760/16151 , C12N2760/16171 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234 , C12Y503/04001 , G06F9/505 , G06F2209/508
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译： 提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。 该方法包括在植物中表达流感HA并通过尺寸排阻色谱法进行纯化。 本发明还涉及包含流感HA蛋白和植物脂质的VLP。 本发明还涉及编码流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。
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6.发明申请
公开(公告)号：WO2010003225A8
公开(公告)日：2010-07-22
申请号：PCT/CA2009000926
申请日：2009-07-02
申请人： MEDICAGO INC , D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
发明人： D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
CPC分类号： C12N15/8257 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/58 , C07K14/005 , C07K2319/02 , C12N7/00 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2830/65
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译： 提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。 该方法包括在植物中表达A / California / 04/09型流感病毒HA，并通过大小排阻色谱法纯化。 本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。 本发明还涉及编码A / California / 04/09型流感病毒的核酸以及载体。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。
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