公开(公告)号：WO2020181354A1

公开(公告)日：2020-09-17

申请号：PCT/CA2019/050319

申请日：2019-03-14

Applicant: MITSUBISHI TANABE PHARMA CORPORATION ,  MEDICAGO INC. ,  NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： LAVOIE, Pierre-Olivier ,  D'AOUST, Marc-Andre ,  KATO, Ko ,  YAMASAKI, Shotaro

IPC: C12N15/113 ,  A01H5/00 ,  C07K14/08 ,  C07K14/11 ,  C12N15/13 ,  C12N15/40 ,  C12N15/44 ,  C12N15/82 ,  C12N5/10 ,  C12P21/02

Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

公开(公告)号：WO2020000101A1

公开(公告)日：2020-01-02

申请号：PCT/CA2019/050893

申请日：2019-06-27

Applicant: MEDICAGO INC.

Inventor： LAVOIE, Pierre-Olivier ,  LORIN, Aurélien ,  DOUCET, Alain ,  D'AOUST, Marc-André ,  COUTURE, Manon

IPC: C12N15/44 ,  A01H5/00 ,  A61K39/145 ,  A61P31/16 ,  A61P37/04 ,  C07K14/11 ,  C07K16/10 ,  C12N15/82 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01

Abstract: The present invention relates to the production of modified influenza viral proteins in plants. More specifically, the present invention relates to producing and increasing influenza virus-like particle (VLP) production in plants, wherein the VLPs comprise the modified influenza viral proteins, such as modified influenza hemagglutinin (HA). The HA protein may comprising an amino acid sequence comprising at least one substitution when compared to a corresponding wildtype amino acid sequence. Further provided are nucleic acid encoding the modified HA protein. Furthermore methods of producing an influenza virus like particle (VLP) and methods of increasing yield of production of an influenza virus like particle (VLP) in a plant, portion of a plant, or a plant cell, are also provided.

公开(公告)号：WO2011035422A1

公开(公告)日：2011-03-31

申请号：PCT/CA2010/001488

申请日：2010-09-21

Applicant: MEDICAGO INC. ,  VEZINA, Louis-Philippe ,  COUTURE, Manon ,  PAQUET, Dany ,  DARGIS, Michele ,  D'AOUST, Marc-Andre

Inventor： VEZINA, Louis-Philippe ,  COUTURE, Manon ,  PAQUET, Dany ,  DARGIS, Michele ,  D'AOUST, Marc-Andre

IPC: C12N7/02 ,  A61K39/12 ,  A61K39/145 ,  A61P31/16 ,  A61P37/04 ,  C12N7/00

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/517 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2760/16123 ,  C12N2760/16134

Abstract: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant- derived VLPs are recovered from the filtered fraction.

Abstract translation: 提供了植物来源的病毒样颗粒（VLPs）的制备方法。 该方法可以包括获得植物或包含原生质体定位的VLP的植物物质，从植物或植物物质产生原生质体/原生质体级分和原生质体级分，以及回收载脂蛋白级分。 载脂蛋白级分包含植物来源的VLP。 或者，VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得，以产生消化部分。 将消化的馏分过滤以产生过滤的馏分，并从过滤的馏分中回收来自植物的VLP。

公开(公告)号：WO2010148511A8

公开(公告)日：2011-03-10

申请号：PCT/CA2010000983

申请日：2010-06-25

Applicant: MEDICAGO INC ,  COUTURE MANON ,  DARGIS MICHELE ,  LAVOIE PIERRE-OLIVIER ,  VEZINA LOUIS-PHILIPPE ,  D AOUST MARC-ANDRE

Inventor： COUTURE MANON ,  DARGIS MICHELE ,  LAVOIE PIERRE-OLIVIER ,  VEZINA LOUIS-PHILIPPE ,  D AOUST MARC-ANDRE

IPC: C07K14/11 ,  A01H5/00 ,  A61K39/145 ,  A61P31/16 ,  C07K19/00 ,  C12N7/00 ,  C12N7/01 ,  C12N15/44 ,  C12N15/82

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/8257 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16234

Abstract: A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

Abstract translation: 提供了一种在植物或植物的一部分内合成嵌合型流感病毒样颗粒（VLP）的方法。 该方法包括在植物或植物的一部分中表达嵌合流感HA。 本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。 本发明还涉及编码嵌合流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

公开(公告)号：WO2008151440A8

公开(公告)日：2010-03-04

申请号：PCT/CA2008001139

申请日：2008-06-13

Applicant: MEDICAGO INC ,  CENTRE NAT RECH SCIENT ,  D AOUST MARC-ANDRE ,  MARQUET-BLOUIN ESTELLE ,  BARDOR MURIEL ,  BUREL CAROLE ,  FAYE LOIC ,  LEROUGE PATRICE ,  VEZINA LOUIS-PHILIPPE ,  GOMORD VERONIQUE ,  AQUIN STEPHANIE ,  RIHOUEY CHRISTOPHE ,  PACCALET THOMAS ,  SOURROUILLE CHRISTOPHE ,  UNIV ROUEN

Inventor： D AOUST MARC-ANDRE ,  MARQUET-BLOUIN ESTELLE ,  BARDOR MURIEL ,  BUREL CAROLE ,  FAYE LOIC ,  LEROUGE PATRICE ,  VEZINA LOUIS-PHILIPPE ,  GOMORD VERONIQUE ,  AQUIN STEPHANIE ,  RIHOUEY CHRISTOPHE ,  PACCALET THOMAS ,  SOURROUILLE CHRISTOPHE

IPC: C12N15/62 ,  A01H5/00 ,  A01H5/10 ,  C07K19/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  C12P21/02 ,  C12P21/08

CPC classification number: C12N15/8257 ,  C12N9/1051 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNTl-GaIT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNTl) fused to a catalytic domain of beta-l,4galactosyltransferase (GaIT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.

Abstract translation: 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化谱的目的蛋白质的方法。 该方法包括在植物内共表达编码编码杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列包含融合到β-1,4galactosyltransferase的催化结构域的N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域 GaIT），与在植物中有活性的第一调节区可操作地连接的第一核苷酸序列和用于编码目标蛋白的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调节区可操作地连接 。 共表达第一和第二核苷酸序列以合成目标蛋白，其包含在植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖。

公开(公告)号：WO2008151440A4

公开(公告)日：2009-02-26

申请号：PCT/CA2008001139

申请日：2008-06-13

Applicant: MEDICAGO INC ,  CENTRE NAT RECH SCIENT ,  D AOUST MARC-ANDRE ,  MARQUET-BLOUIN ESTELLE ,  BARDOR MURIEL ,  BUREL CAROLE ,  FAYE LOIC ,  LEROUGE PATRICE ,  VEZINA LOUIS-PHILIPPE ,  GOMORD VERONIQUE ,  AQUIN STEPHANIE ,  RIHOUEY CHRISTOPHE ,  PACCALET THOMAS ,  SOURROUILLE CHRISTOPHE

Inventor： D AOUST MARC-ANDRE ,  MARQUET-BLOUIN ESTELLE ,  BARDOR MURIEL ,  BUREL CAROLE ,  FAYE LOIC ,  LEROUGE PATRICE ,  VEZINA LOUIS-PHILIPPE ,  GOMORD VERONIQUE ,  AQUIN STEPHANIE ,  RIHOUEY CHRISTOPHE ,  PACCALET THOMAS ,  SOURROUILLE CHRISTOPHE

IPC: C12N15/62 ,  A01H5/00 ,  A01H5/10 ,  C07K19/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  C12P21/02 ,  C12P21/08

CPC classification number: C12N15/8257 ,  C12N9/1051 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNTl-GaIT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNTl) fused to a catalytic domain of beta-l,4galactosyltransferase (GaIT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.

Abstract translation: 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化谱的目的蛋白质的方法。 该方法包括在植物内共表达编码编码杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列包含融合到β-1,4galactosyltransferase的催化结构域的N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域 GaIT），与在植物中有活性的第一调节区可操作地连接的第一核苷酸序列和用于编码目标蛋白的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调节区可操作地连接 。 共表达第一和第二核苷酸序列以合成目标蛋白，其包含在植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖。

公开(公告)号：WO2008151440A1

公开(公告)日：2008-12-18

申请号：PCT/CA2008/001139

申请日：2008-06-13

Applicant: MEDICAGO, INC. ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  D'AOUST, Marc-Andre ,  MARQUET-BLOUIN, Estelle ,  BARDOR, Muriel ,  BUREL, Carole ,  FAYE, Loic ,  LEROUGE, Patrice ,  VEZINA, Louis-Philippe ,  GOMORD, Véronique ,  AQUIN, Stéphanie ,  RIHOUEY, Christophe ,  PACCALET, Thomas ,  SOURROUILLE, Christophe

Inventor： D'AOUST, Marc-Andre ,  MARQUET-BLOUIN, Estelle ,  BARDOR, Muriel ,  BUREL, Carole ,  FAYE, Loic ,  LEROUGE, Patrice ,  VEZINA, Louis-Philippe ,  GOMORD, Véronique ,  AQUIN, Stéphanie ,  RIHOUEY, Christophe ,  PACCALET, Thomas ,  SOURROUILLE, Christophe

IPC: C12N15/62 ,  A01H5/00 ,  A01H5/10 ,  C07K19/00 ,  C12N15/54 ,  C12N15/82 ,  C12N5/10 ,  C12N9/10 ,  C12P21/02 ,  C12P21/08

CPC classification number: C12N15/8257 ,  C12N9/1051 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNTl-GaIT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNTl) fused to a catalytic domain of beta-l,4galactosyltransferase (GaIT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.

Abstract translation: 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化谱的目的蛋白质的方法。 该方法包括在植物内共表达编码编码杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列包含融合到β-1,4galactosyltransferase的催化结构域的N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域 GaIT），与在植物中有活性的第一调节区可操作地连接的第一核苷酸序列和用于编码目标蛋白的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调节区可操作地连接 。 共表达第一和第二核苷酸序列以合成目标蛋白，其包含在植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖。

公开(公告)号：WO2008006224A1

公开(公告)日：2008-01-17

申请号：PCT/CA2007/001255

申请日：2007-07-13

Applicant: Medicago, Inc ,  PELLETIER, Joelle ,  VEZINA, Louis-Philippe ,  LANDRY, Nathalie ,  SAVARD, Sylvain

Inventor： VEZINA, Louis-Philippe ,  LANDRY, Nathalie ,  SAVARD, Sylvain

IPC: A61K31/704 ,  A23L1/30 ,  A61K31/575 ,  A61P3/06

CPC classification number: A61K31/575 ,  A23L33/105 ,  A61K31/704

Abstract: A cholesterol-lowering preparation comprising medicagenic acid saponin is disclosed. The amount of medicagenic acid saponin in the preparation is greater than 50% by weight to produce the cholesterol-lowering effect in an animal. A method of purifying a preparation of at least 30% medicagenic acid saponin is also disclosed. The preparation may be purified from alfalfa plants and used as a treatment for lowering cholesterol and triglycerides in an animal, in particular a human.

Abstract translation: 公开了一种包含姬松茸酸皂苷的降胆固醇制剂。 为了在动物体内产生降胆固醇效果，制剂中的致acid酸皂苷的量大于50重量％。 还公开了纯化至少30％的致acid酸皂苷的制剂的方法。 该制剂可以从苜蓿植物中纯化出来并用作降低动物，特别是人类中的胆固醇和甘油三酯的处理剂。

公开(公告)号：WO2007090290A1

公开(公告)日：2007-08-16

申请号：PCT/CA2007/000197

申请日：2007-02-09

Applicant: MEDICAGO INC. ,  LE CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  PACCALET, Thomas ,  BARDOR, Muriel ,  RIHOUEY, Christophe ,  GOMORD, Véronique ,  FAYE, Loïc ,  LEROUGE, Patrice ,  AQUIN, Stéphanie ,  VEZINA, Louis-Philippe ,  D'AOUST, Marc-André

Inventor： PACCALET, Thomas ,  BARDOR, Muriel ,  RIHOUEY, Christophe ,  GOMORD, Véronique ,  FAYE, Loïc ,  LEROUGE, Patrice ,  AQUIN, Stéphanie ,  VEZINA, Louis-Philippe ,  D'AOUST, Marc-André

IPC: C12N5/10 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  A61K38/14 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N5/04 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02 ,  C12P21/00 ,  C12N9/10

CPC classification number: C12N15/8245 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/8257 ,  C12P19/26 ,  C12P21/005 ,  C12Y205/01057 ,  C12Y401/03003

Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP- Neu5Ac transporter and a sialyltransferase.

Abstract translation: 提供了一种在植物中合成唾液酸的方法，以及能够合成唾液酸的植物。 此外，还提供了在植物中产生唾液酸化蛋白质的方法。 合成唾液酸的方法包括提供包含编码N-乙酰神经氨酸（Neu5Ac）合酶或Neu5Ac裂解酶的核苷酸序列的植物，并表达该核苷酸序列从而合成唾液酸。 植物还可以共表达编码差向异构酶，CMP-Neu5Ac合成酶，CMP-Neu5Ac转运蛋白和唾液酸转移酶中的一种或多种的核苷酸序列。

公开(公告)号：WO2018170603A1

公开(公告)日：2018-09-27

申请号：PCT/CA2018/050352

申请日：2018-03-23

Applicant: MEDICAGO INC.

Inventor： LAVOIE, Pierre-Olivier ,  D'AOUST, Marc-Andre

IPC: C12N15/82 ,  A01H5/00 ,  A61K39/12 ,  A61P31/14 ,  A61P37/04 ,  C07K14/08 ,  C07K16/20 ,  C12N15/40 ,  C12N15/62 ,  C12N15/63 ,  C12N5/10 ,  C12N7/01 ,  C12N7/04 ,  C12P21/02

Abstract: Nucleic acids encoding norovirus VP1 fusion proteins and VLPs comprising the norovirus VP1 fusion proteins are provided. Methods for norovirus VP1 fusion protein and norovirus VLP production in plants are also described. The VP1 fusion protein comprises, a first sequence encoding an S domain derived from a first norovirus strain, and a second sequence encoding a P domain derived from a second norovirus strain.