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    AFFINITY TRAPPING AGENT AND USES THEREOF
    4.
    发明申请
    AFFINITY TRAPPING AGENT AND USES THEREOF 审中-公开
    AFFINITY TRAPPING代理及其用途

    公开(公告)号：WO2012168794A1

    公开(公告)日：2012-12-13

    申请号：PCT/IB2012/001268

    申请日：2012-06-06

    申请人： ANGANY GENETICS ,  MEDICAGO, INC. ,  GOMORD, Véronique ,  FAYE, Loïc ,  D'AOUST, Marc-André ,  COUTURE, Manon ,  TREPANIER, Sonia ,  VEZINA, Louis-Philippe ,  FITCHETTE, Anne Catherine

    发明人： GOMORD, Véronique ,  FAYE, Loïc ,  D'AOUST, Marc-André ,  COUTURE, Manon ,  TREPANIER, Sonia ,  VEZINA, Louis-Philippe ,  FITCHETTE, Anne Catherine

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8258 ,  C12N15/8257

    摘要： A nucleic acid encoding an affinity trapping agent is provided. The affinity trapping agent comprises a peptide or protein ligand having a binding affinity for a protein of interest, fused to a heterologous subcellular compartment targeting signal. Methods for targeting the immobilization of a recombinant peptide or protein of interest, or an endogenous peptide or protein of interest, to a subcellular compartment of a cell are also provided. If the peptide or protein of interest is recombinant then a nucleotide sequence encoding the recombinant peptide or protein of interest and a nucleic acid encoding the affinity trapping agent are introduced into the cell and co- expressed to form a complex thereby targeting the immobilization of the recombinant peptide or protein of interest. If the peptide or protein of interest is endogenous, then the nucleic acid encoding the affinity trapping agent having a binding affinity for the endogenous peptide or protein of interest is introduced into the cell, and the nucleic acid encoding the affinity trapping agent is expressed in the cell to form a complex thereby targeting immobilization of the endogenous peptide or protein of interest.

    摘要翻译： 提供编码亲和性捕获剂的核酸。 亲和捕获剂包括与目标蛋白质结合亲和力的肽或蛋白质配体，其与异源亚细胞区室靶向信号融合。 还提供了将目标重组肽或目的蛋白或感兴趣的内源性肽或蛋白质固定靶向细胞亚细胞室的方法。 如果感兴趣的肽或重组蛋白是重组的，则将编码目标重组肽或蛋白质的核苷酸序列和编码亲和捕获剂的核酸引入细胞并共表达以形成复合物，从而靶向重组体 肽或目的蛋白质。 如果目的肽或蛋白质是内源性的，则将编码对所述目的内源肽或蛋白质具有结合亲和力的亲和捕获剂的核酸引入细胞中，并且编码亲和捕获剂的核酸在 细胞以形成复合物，从而靶向目标的内源性肽或蛋白质的固定化。