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41 条记录 (耗时 0.013 秒)

    INCREASED STABILITY OF A DNA FORMULATION BY INCLUDING POLY-L-GLUTAMATE
    2.
    发明申请
    INCREASED STABILITY OF A DNA FORMULATION BY INCLUDING POLY-L-GLUTAMATE 审中-公开
    通过包含聚-L-谷氨酸的DNA配方的增加的稳定性

    公开(公告)号：WO2009065121A1

    公开(公告)日：2009-05-22

    申请号：PCT/US2008/083785

    申请日：2008-11-17

    申请人： VGX PHARMACEUTICALS, INC. ,  DRAGHIA-AKLI, Ruxandra ,  POPE, Melissa

    发明人： DRAGHIA-AKLI, Ruxandra ,  POPE, Melissa

    IPC分类号： A61K48/00 ,  A61K39/00 ,  C12N15/63

    CPC分类号： A61K48/0008 ,  A61K39/00 ,  A61K2039/53 ,  C12N15/87

    摘要： Aspects of the present invention is related to DNA vaccine formulations having enhanced stability comprising at least one DNA plasmid capable of expressing an antigen in cells of mammal and poly-L-glutamate; wherein the DNA plasmid is present in the vaccine formulation at a concentration of at least 1 mg/ml, and the poly-L-glutamate is present in the amount of weight that is 1 % of the amount of DNA plasmid. Some aspects of the present invention is related to methods of stabilizing DNA plasmid in a DNA vaccine formulation. Additionally, the present invention is related to methods for introducing a DNA vaccine formulation having enhanced stability into a cell of a selected tissue in a recipient.

    摘要翻译： 本发明的方面涉及具有增强的稳定性的DNA疫苗制剂，其包含能够在哺乳动物和聚-L-谷氨酸的细胞中表达抗原的至少一种DNA质粒; 其中所述DNA质粒以至少1mg / ml的浓度存在于所述疫苗制剂中，并且所述多聚-L-谷氨酸盐以所述DNA质粒量的1％的量存在。 本发明的一些方面涉及在DNA疫苗制剂中稳定DNA质粒的方法。 另外，本发明涉及将具有增强的稳定性的DNA疫苗制剂引入受体中选定组织的细胞的方法。

    ANTIBODY PRODUCTION ELICITED BY A DNA VACCINE DELIVERED BY ELECTROPORATION
    3.
    发明申请
    ANTIBODY PRODUCTION ELICITED BY A DNA VACCINE DELIVERED BY ELECTROPORATION 审中-公开
    通过电穿孔传递DNA疫苗引发的抗体生产

    公开(公告)号：WO2009065032A1

    公开(公告)日：2009-05-22

    申请号：PCT/US2008/083631

    申请日：2008-11-14

    申请人： VGX PHARMACEUTICALS, INC. ,  DRAGHIA-AKLI, Ruxandra ,  KHAN, Amir, S.

    发明人： DRAGHIA-AKLI, Ruxandra ,  KHAN, Amir, S.

    IPC分类号： A61K39/12

    CPC分类号： A61K39/12 ,  A61K39/0011 ,  A61K39/145 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/55577 ,  A61K2039/70 ,  C12N13/00 ,  C12N15/87 ,  C12N2740/16134 ,  C12N2760/16134

    摘要： There are provided methods of generating antibodies in a mammal against recombinant antigens using DNA plasmids capable of expressing said antigens in cells of said mammal, comprising: injecting into tissue of said mammal a DNA plasmid comprising an encoding sequence operably linked to a promoter, electroporating said tissue with an electroporation device capable of delivering an electrical pulse effective to electroporate cells of said tissue to allow entry of said DNA plasmid and expression of said antigen, and allowing said mammal to respond to said expressed antigen in order to generate antibodies to said antigen. Furthermore, there are provided methods of isolating antibodies specific against desired antigens wherein said antibodies are generated in a mammal using DNA plasmids capable of expressing said antigens.

    摘要翻译： 提供了使用能够在所述哺乳动物的细胞中表达所述抗原的DNA质粒在哺乳动物中产生针对重组抗原的抗体的方法，所述方法包括：将包含编码序列的DNA质粒注射入所述哺乳动物的组织中 可操作地连接到启动子上，用能够传递有效电脉冲的电穿孔装置电穿孔所述组织，以使所述组织的细胞有效电穿孔以允许所述DNA质粒进入和表达所述抗原，并且允许所述哺乳动物对所述表达的抗原 为了产生针对所述抗原的抗体。 此外，提供了分离针对所需抗原的特异性抗体的方法，其中所述抗体是使用能够表达所述抗原的DNA质粒在哺乳动物中产生的。

    COMPOSITIONS COMPRISING HIGH CONCENTRATION OF BIOLOGICALLY ACTIVE MOLECULES AND PROCESSES FOR PREPARING THE SAME
    4.
    发明申请
    COMPOSITIONS COMPRISING HIGH CONCENTRATION OF BIOLOGICALLY ACTIVE MOLECULES AND PROCESSES FOR PREPARING THE SAME 审中-公开

    公开(公告)号：WO2008148010A9

    公开(公告)日：2008-12-04

    申请号：PCT/US2008/064726

    申请日：2008-05-23

    申请人： VGX PHARMACEUTICALS, INC. ,  DRAGHIA-AKLI, Ruxandra ,  HEBEL, Henry ,  CAI, Ying

    发明人： DRAGHIA-AKLI, Ruxandra ,  HEBEL, Henry ,  CAI, Ying

    IPC分类号： C12N15/10

    摘要： Large scale processes for producing high purity samples of biologically active molecules of interest from bacterial cells are disclosed. The methods comprise the steps of producing a lysate solution by contacting a cell suspension of said plurality of cells with lysis solution; neutralizing said lysate solution with a neutralizing solution to produce a dispersion that comprises neutralized lysate solution and debris; filtering the dispersion through at least one filter; performing ion exchange separation on said neutralized lysate solution to produce an ion exchange eluate; and performing hydrophobic interaction separation on said ion exchange eluate to produce a hydrophobic interaction solution. Further, provided are compositions comprising large scale amounts of plasmid DNA produced by the disclosed large scale processes.

    GROWTH HORMONE RELEASING HORMONE ENHANCES VACCINATION RESPONSE
    10.
    发明申请
    GROWTH HORMONE RELEASING HORMONE ENHANCES VACCINATION RESPONSE 审中-公开
    生长激素释放激素提高疫苗接种反应

    公开(公告)号：WO2006014737A2

    公开(公告)日：2006-02-09

    申请号：PCT/US2005/025747

    申请日：2005-07-21

    申请人： ADViSYS, INC. ,  DRAGHIA-AKLI, Ruxandra ,  BROWN, Patrica, A. ,  KHAN, Amir, S. ,  DAVIS, William, C.

    发明人： DRAGHIA-AKLI, Ruxandra ,  BROWN, Patrica, A. ,  KHAN, Amir, S. ,  DAVIS, William, C.

    IPC分类号： A61K48/00

    CPC分类号： A61K39/39 ,  A61K2039/55516

    摘要： This invention discloses compositions and methods of: vaccinating a subject; enhancing the vaccination efficiency; preparing a subject prior to vaccination response; and improving the clinical outcome after infectious challenge in a subject that has been vaccinated. More specifically, the invention pertains to delivering into a tissue of the subject a nucleic acid expression construct that encodes a growth-hormone-releasing-hormone ("GHRH") before or concomitantly with delivering a vaccine to the subject, wherein, GHRH is expressed in vivo in the subject, wherein the subject comprises a human, pig, cow, bird, horse or any other animal species receiving a vaccine.

    摘要翻译： 本发明公开了一种组合物和方法：接种受试者; 提高疫苗接种效率; 在接种疫苗接种前准备受试者; 并且在接种疫苗的受试者中感染性挑战后改善临床结果。 更具体地，本发明涉及在受试者的组织中递送编码生长激素释放激素（“GHRH”）的核酸表达构建体，或者同时向受试者递送疫苗，其中GHRH被表达 在受试者体内，其中受试者包括人，猪，牛，鸟，马或接受疫苗的任何其他动物物种。