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    METHOD FOR HARVESTING EXPRESSION PRODUCTS
    3.
    发明申请
    METHOD FOR HARVESTING EXPRESSION PRODUCTS 审中-公开
    收集表达产品的方法

    公开(公告)号：WO2012010280A1

    公开(公告)日：2012-01-26

    申请号：PCT/EP2011/003552

    申请日：2011-07-15

    申请人： BAVARIAN NORDIC A/S ,  WEHNES, Engelbert ,  WERNER, Uwe ,  LESCHKE, Christian

    发明人： WEHNES, Engelbert ,  WERNER, Uwe ,  LESCHKE, Christian

    IPC分类号： C12N7/02 ,  C12N15/10

    CPC分类号： C12N7/02 ,  C12M47/02 ,  C12N7/00 ,  C12N2710/24151

    摘要： The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA.

    摘要翻译： 本发明提供了从宿主细胞中回收基本上与细胞相关的表达产物的方法，包括（a）在允许所述表达产物表达的条件下培养所述宿主细胞; （b）在过滤器单元中/之上收集所述宿主细胞; （c）在所述过滤器单元中/之上破坏所述宿主细胞; 和（d）从所述破坏的宿主细胞中分离所述表达产物。 所述宿主细胞优选是脊椎动物细胞，更优选禽类细胞，其优选以悬浮培养。 此外，本发明提供了一种过滤器单元的使用，其特征在于所述过滤器单元是（i）适于保留表达表达产物的宿主细胞; 和（ii）适合于在所述过滤器单元中/之后的细胞破碎之后从所述过滤器单元洗脱所述表达产物，用于从所述宿主细胞回收所述表达产物，以及用于从包含所述宿主细胞的宿主细胞中回收表达产物的系统 过滤器单元 本发明还提供可通过所述方法获得的表达产物，所述表达产物优选病毒，特别是痘病毒，特别是选自禽痘病毒，痘苗病毒，更优选修饰的痘苗病毒安卡拉，MVA。

    VECTOR COMPRISING MULTIPLE HOMOLOGOUS NUCLEOTIDE SEQUENCES
    5.
    发明申请
    VECTOR COMPRISING MULTIPLE HOMOLOGOUS NUCLEOTIDE SEQUENCES 审中-公开
    包含多个同源核苷酸序列的载体

    公开(公告)号：WO2010057650A1

    公开(公告)日：2010-05-27

    申请号：PCT/EP2009/008275

    申请日：2009-11-20

    申请人： BAVARIAN NORDIC A/S ,  STEIGERWALD, Robin

    发明人： STEIGERWALD, Robin

    IPC分类号： C12N15/863

    CPC分类号： C12N15/8636 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/11022 ,  C12N2760/14122 ,  C12N2799/02 ,  C12N2800/22

    摘要： The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.

    摘要翻译： 本发明涉及包含两个或多个同源核苷酸序列的载体和用于产生它们的方法。 本发明涉及用不改变编码氨基酸序列的不同碱基取代同源核苷酸序列中的碱基。 本发明允许减少同源核苷酸序列之间的分子内重组，特别是在哺乳动物细胞中。 本发明还涉及含有取代碱基的核苷酸序列。

    DEPLETION OF HOST CELL COMPONENTS FROM LIVE VIRUS VACCINES
    9.
    发明申请
    DEPLETION OF HOST CELL COMPONENTS FROM LIVE VIRUS VACCINES 审中-公开
    从活的病毒疫苗中去除宿主细胞成分

    公开(公告)号：WO2012069190A3

    公开(公告)日：2012-11-15

    申请号：PCT/EP2011005901

    申请日：2011-11-23

    申请人： PROBIOGEN AG ,  JORDAN INGO ,  BERNHARDT HOLGER ,  HARTMANN STEFAN

    发明人： JORDAN INGO ,  BERNHARDT HOLGER ,  HARTMANN STEFAN

    IPC分类号： C12N7/02

    CPC分类号： C12N7/02 ,  C12N2710/24151

    摘要： It is desirable to produce live vaccines, which are highly attenuated and which do only contain minimal or no animal-derived components. The production of highly attenuated live viruses can be better achieved when using specifically designed cell lines as producer substrate as opposed to using less defined primary cells. However, live viruses, thus produced comprise undesirable components from the cell lines and cell culture. The present invention relates to methods of production and purification of live enveloped viruses, which are suitable for vaccination.

    摘要翻译： 需要生产活性疫苗，其是高度减毒的，并且仅包含最少的或不含动物衍生的组分。 当使用特定设计的细胞系作为生产者底物而不是使用较少限定的原代细胞时，可以更好地实现高度减毒的活病毒的产生。 然而，由此产生的活病毒包含来自细胞系和细胞培养物的不良成分。 本发明涉及适用于疫苗接种的活包膜病毒的生产和纯化方法。