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    PROTEASE FOR WOUND CONDITIONING AND SKIN CARE
    1.
    发明申请
    PROTEASE FOR WOUND CONDITIONING AND SKIN CARE 审中-公开
    蛋白酶对伤口调理和皮肤护理

    公开(公告)号：WO2010099955A1

    公开(公告)日：2010-09-10

    申请号：PCT/EP2010/001328

    申请日：2010-03-03

    Applicant: B.R.I.A.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG ,  NIEHAUS, Frank ,  ECK, Jürgen ,  SCHULZE, Renate

    Inventor： NIEHAUS, Frank ,  ECK, Jürgen ,  SCHULZE, Renate

    IPC: C12N9/64 ,  C07K16/40 ,  A61K38/43 ,  C12N15/62 ,  C12N5/10

    CPC classification number: C12N9/6408

    Abstract: We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80 % identical to the amino acid sequence of (a), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80 % identical to the nucleotide sequence of (b), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

    Abstract translation: 我们已经通过分子克隆鉴定了来源于丝光绿蝇幼虫的蛋白酶，并且由于其活性可用于伤口清创，因此被称为清创酶。 描述了编码丝氨酸蛋白酶的核酸分子，所述丝氨酸蛋白酶具有切割纤维蛋白和酪蛋白的能力，所述纤维蛋白和酪蛋白是（a）编码包含SEQ ID NO：4的氨基酸序列或由SEQ ID NO：4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子， 编码所述丝氨酸蛋白酶的前体或片段的酸分子; （b）包含SEQ ID NO：3的核苷酸序列或由其组成的核酸分子; （c）编码丝氨酸蛋白酶的核酸分子，其氨基酸序列与（a）的氨基酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，且大多数 优选的95％相同; （d）包含与（b）的核苷酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，并且最优选95％相同的核苷酸序列或由其组成的核酸分子， 相同; （e）相对于（b）或（d）的核酸分子简并的核酸分子; 或（f）对应于（a）至（d）中任一项的核酸分子的核酸分子，其中T被U取代。

    ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS
    4.
    发明申请
    ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 审中-公开
    来自未受污染的生物体的DNA的分离和克隆

    公开(公告)号：WO2004018673A2

    公开(公告)日：2004-03-04

    申请号：PCT/EP2003/009223

    申请日：2003-08-20

    Applicant: B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG ,  QUAISER, Achim ,  OCHSENREITER, Torsten ,  TREUSCH, Alexander, H. ,  KLETZIN, Arnulf ,  SCHLEPER, Christa ,  LORENZ, Patrick ,  ECK, Jürgen

    Inventor： QUAISER, Achim ,  OCHSENREITER, Torsten ,  TREUSCH, Alexander, H. ,  KLETZIN, Arnulf ,  SCHLEPER, Christa ,  LORENZ, Patrick ,  ECK, Jürgen

    IPC: C12N15/10

    CPC classification number: C12N15/1003

    Abstract: The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

    Abstract translation: 本发明涉及用于分离和/或纯化核酸分子的装置，所述核酸分子适于结合和/或失活用于DNA操作的试剂或酶的活性的抑制剂并分离多个 的核酸分子的大小。 此外，本发明涉及用于分离核酸分子的方法，其包括将样品应用于本发明的装置，其中所述核酸分子优选代表给定栖息地的宏基因组的一部分。 此外，本发明涉及产生至少一种基因文库的方法，所述基因文库包含通过本发明方法分离的核酸分子和通过本发明方法分离的核酸分子。

    NEUE GLYKOSYLHYDROLASEN
    7.
    发明申请
    NEUE GLYKOSYLHYDROLASEN 审中-公开
    新糖

    公开(公告)号：WO2003054177A2

    公开(公告)日：2003-07-03

    申请号：PCT/EP2002/014210

    申请日：2002-12-13

    Applicant: HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN ,  BREVES, Roland ,  MAURER, Karl-Heinz ,  ECK, Jürgen ,  LORENZ, Patrick ,  ZINKE, Holger

    Inventor： BREVES, Roland ,  MAURER, Karl-Heinz ,  ECK, Jürgen ,  LORENZ, Patrick ,  ZINKE, Holger

    IPC: C12N9/00

    CPC classification number: C12N9/2414 ,  C07K2319/00 ,  C11D3/386 ,  Y02E50/17

    Abstract: Die vorliegende Erfindung betrifft neue Glykosylhydrolasen mit amylolytischer Aktivität und die für diese Glykosylhydrolasen codierenden Nukleinsäuren, ein PCR-basiertes Verfahren zur Identifizierung und Gewinnung neuer Glykosyl­hydrolasen aus Metagenom-DNA sowie diverse technische Anwendungsmöglich­keiten für derartige Glykosylhydrolasen mit amylolytischer Aktivität. Hierunter sind Wasch- und Reinigungsmittel mit solchen Enzymen sowie entsprechende Verfah­ren und Verwendungsmöglichkeiten von besonderem Interesse.

    Abstract translation: 本发明涉及具有淀粉分解活性的新的糖基水解酶和编码这些糖基水解酶，用于鉴定和获得来自宏基因组DNA，以及用于与淀粉分解活性，例如糖基水解酶的各种技术应用的新糖基水解酶基于PCR的方法中的核酸。 其中，洗涤剂和清洁产品，例如酶和相应的方法和特别感兴趣的用途。

    NOVEL CATALASES
    8.
    发明申请
    NOVEL CATALASES 审中-公开
    新品推荐

    公开(公告)号：WO2012072777A1

    公开(公告)日：2012-06-07

    申请号：PCT/EP2011/071576

    申请日：2011-12-01

    Applicant: B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG ,  SCHULZE, Renate ,  ECK, Jürgen

    Inventor： SCHULZE, Renate ,  ECK, Jürgen

    IPC: A61K8/66 ,  A61K38/44 ,  A61L12/08 ,  A61Q19/08 ,  C11D3/386 ,  C12N9/08

    CPC classification number: C12N9/0065 ,  A61K8/606 ,  A61K8/66 ,  A61K38/00 ,  A61Q5/10 ,  A61Q19/08 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/41 ,  C07K2319/42 ,  C07K2319/43 ,  C07K2319/60 ,  C12Y111/01006 ,  D06L4/40 ,  D21C5/005 ,  D21H17/005

    Abstract: The invention relates to a nucleic acid molecule encoding a polypeptide having the activity of a catalase (EC 1.1 1.1.6), which nucleic acid molecule is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule comprising or consisting of a nucleic acid molecule encoding a polypeptide having the activity of a catalase the amino acid sequence of which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, (d) a nucleic acid molecule encoding a polypeptide having the activity of a catalase and comprising or consisting of a nucleotide sequence which is at least 98% identical to the nucleotide sequence of SEQ ID NO: 2, (e) a fragment of the polypeptide of any of (a) to (d) having the activity of a catalase and comprising at least 675 amino acids; or (f) the nucleic acid sequence of any of (a) to (d) wherein T is U.

    Abstract translation: 本发明涉及编码具有过氧化氢酶活性的多肽的核酸分子（EC 1.1 1.1.6），该核酸分子是（a）编码包含SEQ ID NO：1所示氨基酸序列或由SEQ ID NO： ID NO：1; （b）包含SEQ ID NO：2的核苷酸序列或由其组成的核酸分子; （c）核酸分子，其包含编码具有与SEQ ID NO：1的氨基酸序列至少99％相同的氨基酸序列的过氧化氢酶活性的多肽的核酸分子，（d ）编码具有过氧化氢酶活性并且由与SEQ ID NO：2的核苷酸序列至少98％相同的核苷酸序列组成或由其组成的核酸分子，（e）任何 （a）至（d）具有过氧化氢酶的活性并且包含至少675个氨基酸; 或（f）（a）至（d）中任一项的核酸序列，其中T为U.

    A NOVEL GLUCOSE OXIDASE
    9.
    发明申请
    A NOVEL GLUCOSE OXIDASE 审中-公开
    新型葡萄糖氧化酶

    公开(公告)号：WO2012017008A1

    公开(公告)日：2012-02-09

    申请号：PCT/EP2011/063368

    申请日：2011-08-03

    Applicant: B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG ,  SCHWANEBERG, Ulrich ,  PRODANOVIC, Radivoje ,  OSTAFE, Raluca ,  NIEHAUS, Frank ,  ECK, Jürgen

    Inventor： SCHWANEBERG, Ulrich ,  PRODANOVIC, Radivoje ,  OSTAFE, Raluca ,  NIEHAUS, Frank ,  ECK, Jürgen

    IPC: C12N9/04

    CPC classification number: C12N9/0006

    Abstract: The present invention relates to a novel nucleic acid molecule encoding (I) a polypeptide having the activity of a glucose oxidase (EC 1.1.3.4); or (II) a fragment of the polypeptide of (I) having the activity of a glucose oxidase and comprising at least 400 amino acids. Moreover, the invention relates to a vector encoding the nucleic acid molecule, a host cell transformed, transduced or transfected with the vector, a the protein encoded by the nucleic acid molecule, and compositions comprising said nucleic acid molecule, vector, host cell, protein or combinations thereof.

    Abstract translation: 本发明涉及编码（I）具有葡萄糖氧化酶活性的多肽的新型核酸分子（EC 1.1.3.4）; 或（II）具有葡萄糖氧化酶活性且包含至少400个氨基酸的（I）多肽的片段。 此外，本发明涉及编码核酸分子的载体，用载体转化，转导或转染的宿主细胞，由核酸分子编码的蛋白质，以及包含所述核酸分子，载体，宿主细胞，蛋白质的组合物 或其组合。

    A NOVEL METHOD FOR THE PRODUCTION OF A ANTIMICROBIAL PEPTIDE
    10.
    发明申请
    A NOVEL METHOD FOR THE PRODUCTION OF A ANTIMICROBIAL PEPTIDE 审中-公开
    一种生产抗微生物肽的新方法

    公开(公告)号：WO2011036174A1

    公开(公告)日：2011-03-31

    申请号：PCT/EP2010/063971

    申请日：2010-09-22

    Applicant: B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG ,  NAUMER, Christian ,  KROHN, Michael ,  TIFFERT, Yvonne ,  ECK, Jürgen

    Inventor： NAUMER, Christian ,  KROHN, Michael ,  TIFFERT, Yvonne ,  ECK, Jürgen

    IPC: A61K38/17 ,  C07K14/47 ,  C12P21/00

    CPC classification number: C07K14/4703 ,  A61K38/4873 ,  C12P21/06 ,  Y02A50/401 ,  A61K2300/00

    Abstract: The present invention relates to a method of producing a peptide consisting of the amino acids 63 to 110 of dermcidin (SEQ ID NO: 3) comprising (a) culturing a host cell carrying a nucleic acid molecule encoding the peptide in an expressible form, and (b) optionally isolating the peptide from the culture. Furthermore, the invention relates to a nucleic acid molecule encoding a fusion protein comprising or consisting of (a) a peptide heterologous with regard to dermcidin protein-tag; and, C-terminally thereof (b) a peptide having the antimicrobial activity of dermcidin wherein the fusion protein contains an arginine residue located immediately N-terminally of the peptide of (b).

    Abstract translation: 本发明涉及一种由皮霉素（SEQ ID NO：3）的氨基酸63至110组成的肽的方法，该方法包括：（a）培养携带编码可表达形式的肽的核酸分子的宿主细胞，以及 （b）任选地从培养物中分离肽。 此外，本发明涉及编码融合蛋白的核酸分子，其包含或由（a）针对皮肤素蛋白标签异源的肽组成或由其组成; 及其C-末端（b）具有抗皮肤杀菌活性的肽，其中所述融合蛋白含有位于（b）的肽的N-末端的精氨酸残基。

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