公开(公告)号：WO1986006412A1

公开(公告)日：1986-11-06

申请号：PCT/US1986000742

申请日：1986-04-11

Applicant: GENETICS INSTITUTE, INC. ,  FRITSCH, Edward, Francis ,  COLLINS, Mary

Inventor： GENETICS INSTITUTE, INC.

IPC: C12Q01/68

CPC classification number: C12Q1/682 ,  C12Q1/6813 ,  C12Q1/6823 ,  C12Q1/683 ,  C12Q1/6876

Abstract: A nucleic acid construct useful in preparing reagents for determining target nucleotide sequences in the nucleic acid of a biological sample, the construct having in its single-stranded form: (a) a target binding region substantially complementary to the taget nucleotide sequences and (b) a signal strand pairing segment bound in the construct by complementary base pairing to a portion of the target binding region; a second portion of the target binding region being single-stranded; and the target binding region and signal strand pairing segment being covalently linked by a phosphate/sugar backbone. A replicable nucleic acid having an origin of replication and two half-restriction sites capable of forming a restriction site can be treated with a restriction enzyme to form a length of nucleic acid containing the target binding region and the signal strand pairing segment. Subsequent labeling of the construct and various optional cleavage and derivation steps can convert the construct to a reagent complex.

Abstract translation: 一种用于制备用于测定生物样品核酸中的靶核苷酸序列的试剂的核酸构建体，该构建体具有单链形式：（a）与该标签核苷酸序列基本互补的靶结合区和（b） 通过与靶结合区域的一部分的互补碱基配对结合在构建体中的信号链配对区段; 目标结合区域的第二部分是单链的; 并且靶结合区和信号链配对片段由磷酸/糖主链共价连接。 可以用限制酶处理具有复制起点的复制核酸和两个能够形成限制性位点的半限制性位点，以形成一定长度的含有靶结合区和信号链配对片段的核酸。 随后标记构建体和各种任选的切割和衍生步骤可将构建体转化为试剂复合物。

公开(公告)号：WO2009088968A2

公开(公告)日：2009-07-16

申请号：PCT/US2009/000016

申请日：2009-01-05

Applicant: THE GENERAL HOSPITAL CORPORATION ,  GETMANOVA, Elena, V. ,  KOVTUN, Alexander ,  SUN, Lin ,  FRITSCH, Edward ,  SEED, Brian

Inventor： GETMANOVA, Elena, V. ,  KOVTUN, Alexander ,  SUN, Lin ,  FRITSCH, Edward ,  SEED, Brian

IPC: C07K14/435 ,  C12N9/10

CPC classification number: C12N9/1044

Abstract: Disclosed herein are methods and compositions related to engineered fragments of the human transglutaminase-related protein family, described herein as engineered transglutaminase barrel proteins (ETBPs), that have utility as high affinity, high selectivity target-binding proteins offering advantages as antibody equivalents for therapeutic, analytical, manufacturing and research purposes. ETBPs differ from naturally occurring human transglutaminase fragments by the addition, deletion, replacement and/or substitution of the naturally occurring amino acid sequence. ETBPs can be easily expressed in prokaryotic cells and in many cases can be purified by a simple solubilization and precipitation method.

Abstract translation: 本文公开了与人转谷氨酰胺酶相关蛋白家族的工程化片段相关的方法和组合物，其被描述为工程化转谷氨酰胺酶桶蛋白质（ETBP），其具有作为高亲和力，高选择性靶结合蛋白的效用，作为治疗用抗体的抗体等同物 ，分析，制造和研究目的。 通过天然存在的氨基酸序列的添加，缺失，替换和/或取代，ETBPs与天然存在的人转谷氨酰胺酶片段不同。 ETBPs可以容易地在原核细胞中表达，并且在许多情况下可以通过简单的溶解和沉淀方法纯化。

公开(公告)号：WO2018140391A1

公开(公告)日：2018-08-02

申请号：PCT/US2018/014831

申请日：2018-01-23

Applicant: THE BROAD INSTITUTE, INC. ,  FRITSCH, Edward

Inventor： FRITSCH, Edward

IPC: C12Q1/6858

Abstract: In one aspect, the invention features a combination of oligonucleotides comprising a forward primer oligonucleotide and a blocking oligonucleotide. The forward primer oligonucleotide has a 3' end region, where the 3' end region includes a portion complementary to a mutation positioned in a region within a polynucleotide. The blocking oligonucleotide contains a blocking moiety and has a 5' end region, where the 5' end region includes a portion complementary to a wild-type sequence of the region corresponding to the position of the mutation. In other aspects, the invention provides kits including the combination of primer oligonucleotides and methods of using the oligonucleotides to detect a mutation in a polynucleotide.

公开(公告)号：WO2021072075A1

公开(公告)日：2021-04-15

申请号：PCT/US2020/054785

申请日：2020-10-08

Applicant: FRITSCH, Edward

Inventor： FRITSCH, Edward

IPC: A61K39/00 ,  C07K14/005

Abstract: Disclosed herein is a protein fusion technology that allows the combination of one or more cancer vaccine epitopes with scaffold domains. Also disclosed herein are polypeptide and polynucleic acid compositions encompassed by the protein fusion technology and the methods of using the same.

公开(公告)号：WO2009088968A3

公开(公告)日：2009-10-08

申请号：PCT/US2009000016

申请日：2009-01-05

Applicant: GEN HOSPITAL CORP ,  GETMANOVA ELENA V ,  KOVTUN ALEXANDER ,  SUN LIN ,  FRITSCH EDWARD ,  SEED BRIAN

Inventor： GETMANOVA ELENA V ,  KOVTUN ALEXANDER ,  SUN LIN ,  FRITSCH EDWARD ,  SEED BRIAN

IPC: C07K14/435 ,  C12N9/10

CPC classification number: C12N9/1044

Abstract: Disclosed herein are methods and compositions related to engineered fragments of the human transglutaminase-related protein family, described herein as engineered transglutaminase barrel proteins (ETBPs), that have utility as high affinity, high selectivity target-binding proteins offering advantages as antibody equivalents for therapeutic, analytical, manufacturing and research purposes. ETBPs differ from naturally occurring human transglutaminase fragments by the addition, deletion, replacement and/or substitution of the naturally occurring amino acid sequence. ETBPs can be easily expressed in prokaryotic cells and in many cases can be purified by a simple solubilization and precipitation method.

Abstract translation: 本文公开的是涉及到人的转谷氨酰胺酶相关蛋白家族的工程改造的片段的方法和组合物，本文所述经改造的转谷氨酰胺酶桶蛋白（ETBPs），即具有作为高亲和力，高的选择性靶标结合蛋白提供的优点抗体等同物用于治疗 ，分析，制造和研究目的。 ETBP与天然存在的人转谷氨酰胺酶片段的不同之处在于天然存在的氨基酸序列的添加，缺失，置换和/或取代。 ETBPs可以在原核细胞中容易地表达，并且在许多情况下可以通过简单的溶解和沉淀方法纯化。

公开(公告)号：WO1986003520A1

公开(公告)日：1986-06-19

申请号：PCT/US1985002405

申请日：1985-12-03

Applicant: GENETICS INSTITUTE, INC. ,  FRITSCH, Edward ,  HEWICK, Rodney, M. ,  JACOBS, Kenneth

Inventor： GENETICS INSTITUTE, INC.

IPC: C12P21/00

CPC classification number: C07K14/505 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/44

Abstract: Cloned genes for human erythropoietin (EPO) obtained from human fetal liver that provide surprisingly high levels of expression. Also described is the expression of said genes in vitro to produce active human EPO.

Abstract translation: 从人胎肝获得的人促红细胞生成素（EPO）的克隆基因提供惊人的高水平表达。 还描述了所述基因在体外的表达以产生活性人EPO。