ENZYMATIC HYDROLYSIS OF STARCH
    3.
    发明申请
    ENZYMATIC HYDROLYSIS OF STARCH 审中-公开
    淀粉酶水解

    公开(公告)号:WO2008055138A3

    公开(公告)日:2008-08-14

    申请号:PCT/US2007082968

    申请日:2007-10-30

    CPC classification number: C12P19/14 C12P19/20 C12P19/22

    Abstract: Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or ß-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca ++ ). In certain embodiments, the method comprises contacting the starch substrate with a ß- amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg ++ ), strontium ion (Sr ++ ), barium ion (Ba4 + ) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li + ), potassium ion (K + ) or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.

    Abstract translation: 本文提供了增加淀粉底物的酶水解速率的方法。 在某些实施方案中,该方法包括在大于0.001mM锰离子存在下使淀粉底物与一种或多种淀粉酶家族酶接触。 在某些实施方案中,该方法包括在大于0.001mM钙离子(Ca ++)的存在下使淀粉底物与一种或多种葡糖淀粉酶和/或β-淀粉酶接触。 在某些实施方案中,该方法包括在大于0.001mM的锰离子,钙离子,镁离子(Mg ++),锶离子(Sr < SUP + + + +),钡离子(Ba 4+ +)或所述金属离子的任何组合。 在某些实施方案中,所述方法包括在大于0.001mM的锰离子,钙离子,锂离子(Li +)和/或钾离子(K +)的存在下使淀粉底物与葡糖淀粉酶接触, / SUP>)或所述金属离子的任何组合。 本文还提供了用于水解淀粉的组合物和试剂盒。

    ENZYMATIC HYDROLYSIS OF STARCH
    4.
    发明申请
    ENZYMATIC HYDROLYSIS OF STARCH 审中-公开
    淀粉酶水解

    公开(公告)号:WO2008055138A2

    公开(公告)日:2008-05-08

    申请号:PCT/US2007/082968

    申请日:2007-10-30

    CPC classification number: C12P19/14 C12P19/20 C12P19/22

    Abstract: Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or β-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca ++ ). In certain embodiments, the method comprises contacting the starch substrate with a β- amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg ++ ), strontium ion (Sr ++ ), barium ion (Ba4 + ) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li + ), potassium ion (K + ) or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.

    Abstract translation: 本文提供了提高淀粉底物的酶促水解速率的方法。 在某些实施方案中,所述方法包括在存在大于0.001mM锰离子的情况下使淀粉底物与一种或多种淀粉酶家族酶接触。 在某些实施方案中,该方法包括使淀粉底物与一种或多种葡糖淀粉酶和/或β-淀粉酶在大于0.001mM钙离子(Ca ++)的存在下接触。 在某些实施方案中,该方法包括使淀粉底物与β-淀粉酶在大于0.001mM锰离子,钙离子,镁离子(Mg ++),锶离子(Sr 2+) 钡离子(Ba 4 +)或所述金属离子的任何组合。 在某些实施方案中,所述方法包括使淀粉底物与葡糖淀粉酶在大于0.001mM锰离子,钙离子,锂离子(Li +),钾离子(K + )或所述金属离子的任何组合。 本文还提供了用于水解淀粉的组合物和试剂盒。

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