摘要:
The present invention relates to a method for producing a food product comprising hydrolysed starch, as well as to products obtainable by the method. The method has the advantage of increasing the amount of sugar (i.e.maltose)produced by hydrolysis as compared to conventional methods of starch hydrolysis and present the additional advantage of providing good processability for the food product.
摘要:
The present teachings provide a one step process for producing high maltose syrup. The process comprises contacting starch substrate with a starch liquefying thermostable alpha amylase (s) and a thermoresistant maltose-producing enzyme at a temperature zero to thirty degree above the starch gelatinization temperature resulting in simultaneously solubilizing and hydrolyzing the starch substrate to a high maltose syrup. In some embodiments, the thermoresistant maltose-producing enzyme is a recombinantly-expressed TthAmy
摘要:
The present invention relates to a process for producing a syrup which is rich in maltitol. The process is comprising the successive steps of liquefaction of a starch milk and saccharification of the liquefied starch milk in the presence of alpha-amylase, beta-amylase and a debranching enzyme selected from the group of pullulanase, iso-amylase and mixtures thereof, preferably pullulanase and further adding maltogenic alpha-amylase and/or iso-amylase, for obtaining a maltose containing syrup comprising at least 85% maltose based on dry matter and less than 1.5% glucose based on dry matter, preferably less than 1 % glucose based on dry matter, followed by molecular sieving of the maltose containing syrup to obtain a fraction (A) comprising at least 95% maltose based on dry substance of fraction (A), and further hydrogenating catalytically fraction (A) for obtaining a liquid maltitol enriched product (B).
摘要:
The present invention relates to a process for producing a syrup which is rich in maltitol. The process is comprising the successive steps of liquefaction of a starch milk and saccharification of the liquefied starch milk in the presence of alpha-amylase, beta-amylase and a debranching enzyme selected from the group of pullulanase, iso-amylase and mixtures thereof, preferably pullulanase and further adding maltogenic alpha-amylase and/or iso-amylase, for obtaining a maltose containing syrup comprising at least 85% maltose based on dry matter and less than 1.5% glucose based on dry matter, preferably less than 1 % glucose based on dry matter, followed by molecular sieving of the maltose containing syrup to obtain a fraction (A) comprising at least 95% maltose based on dry substance of fraction (A), and further hydrogenating catalytically fraction (A) for obtaining a liquid maltitol enriched product (B).
摘要:
A method for producing a hydrolysate from lignocellulosic materials generally includes fiberizing the lignocellulosic materials, separating the lignocellulosic materials into at least a first portion and a second portion, wherein at least the first portion includes lignin, treating the first portion to deactivate at least a portion of the lignin in the first portion, re-combining the first and second portions after treating the first portion, and hydrolyzing the lignocellulosic materials with enzymes to produce a hydrolysate.
摘要:
Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or ß-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca ++ ). In certain embodiments, the method comprises contacting the starch substrate with a ß- amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg ++ ), strontium ion (Sr ++ ), barium ion (Ba4 + ) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li + ), potassium ion (K + ) or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.
摘要:
The present invention relates to a method for producing a food product comprising hydrolysed starch, as well as to products obtainable by the method. The method has the advantage of reducing the amount of sugar (i.e.maltose)produced by hydrolysis as compared to conventional methods of starch hydrolysis and present the additional advantage of providing good processability for the food product.
摘要:
The present invention provides enzymes that have been optimized by implementation of Protein Repair One Stop Shop (PROSS), an algorithm that generates protein design(s) for enhanced stability without changing either enzymatic properties or enzyme active site conformation of the respective enzyme. The protein design(s) generated by PROSS introduce mutations to the amino acid sequence of a wild-type protein, resulting in a mutated amino acid sequence that encodes a variant of the wild-type enzyme, i.e., an enzyme variant, which has an enhanced stability, core packing, surface polarity and backbone rigidity, a higher functional expression, and/or a combination thereof, compared to the stability core packing, surface polarity and backbone rigidity, functional expression and/or a combination thereof, of the wild-type enzyme.
摘要:
The present invention provides a process of liquefying starch-containing material comprising the step of treating said starch-containing material with at least one alpha-amylase and a maltogenic amylase or at least one amylase and at least one esterase.