公开(公告)号：WO2009105648A3

公开(公告)日：2009-11-19

申请号：PCT/US2009034685

申请日：2009-02-20

Applicant: GREAT BASIN SCIENT ,  JENISON ROBERT DELMAR

Inventor： JENISON ROBERT DELMAR

IPC: C12Q1/68 ,  B01J19/00

CPC classification number: C12Q1/6834 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C12Q1/6844 ,  C12Q2527/107 ,  C12Q2523/313 ,  C12Q2521/513 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2537/101 ,  C12Q2565/519

Abstract: Compositions and methods for amplifying and detecting solution-state polynucleotide targets in a single device are described. In one aspect, a method for a coupled isothermal amplification and detection process utilizes a coated solid support, including a solid substrate, a cationic layer, and a plurality of target-specific probes attached to the coated solid support. Polynucleotide targets in the sample are amplified by an isothermal amplification process involving in situ hybridization onto the coated solid support. The entire process can be carried out with a high degree of specificity under low salt conditions in less than one hour. Further aspects of the present invention include methods for coupled hybridization/detection of polynucleotide targets, coated silicon biosensors optimized for use with the coupled detection systems to provide visual detection of polynucleotide targets under visible light conditions, and kits for practicing the above described methods.

Abstract translation: 描述了用于在单个装置中扩增和检测溶液状态多核苷酸靶标的组合物和方法。 在一个方面，用于偶联等温放大和检测方法的方法利用包被的固体支持物，包括固体底物，阳离子层和连接到涂覆的固体支持物上的多个靶特异性探针。 样品中的多核苷酸靶标通过等温扩增过程进行扩增，包括原位杂交到涂覆的固体支持物上。 整个过程可以在低盐条件下在低于1小时内以高度的特异性进行。 本发明的其它方面包括用于多核苷酸靶的偶联杂交/检测的方法，优化用于与偶联检测系统一起使用的包被的硅生物传感器以提供在可见光条件下的多核苷酸靶的视觉检测，以及用于实践上述方法的试剂盒。

公开(公告)号：WO2009070742A3

公开(公告)日：2009-07-16

申请号：PCT/US2008084990

申请日：2008-11-26

Applicant: GREAT BASIN SCIENT ,  JENISON ROBERT D ,  KLONOSKI JOSHUA

Inventor： JENISON ROBERT D ,  KLONOSKI JOSHUA

IPC: G01N33/548 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N33/549

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for an analyte, using an amplification polymer that multivalently binds to one or more non-analyte-specific binding site of the multivalent bridge conjugate, if present on the solid support.

Abstract translation: 公开的方法和材料涉及用于分析物诊断测定中扩增信号的改进方法，使用多价结合多价桥缀合物的一个或多个非分析物特异性结合位点的扩增聚合物（如果存在于 坚实的支持。

公开(公告)号：WO2009070640A3

公开(公告)日：2009-09-03

申请号：PCT/US2008084790

申请日：2008-11-26

Applicant: GREAT BASIN SCIENT ,  JENISON ROBERT D ,  KLONOSKI JOSHUA ,  TORRES ANTHONY R

Inventor： JENISON ROBERT D ,  KLONOSKI JOSHUA ,  TORRES ANTHONY R

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.

Abstract translation: 公开了关于用于扩增核酸诊断测定中的信号的改进方法的方法和材料，其包括提供与核酸分析物结合的扩增聚合物的步骤，其中所述扩增聚合物包含多个胺基团; 在扩增聚合物上具有可检测标记复合物的结合胺基; 并在高盐条件下使乙酰化化合物与未被可检测标记物复合物结合的胺基反应。