公开(公告)号：WO2009070742A3

公开(公告)日：2009-07-16

申请号：PCT/US2008084990

申请日：2008-11-26

Applicant: GREAT BASIN SCIENT ,  JENISON ROBERT D ,  KLONOSKI JOSHUA

Inventor： JENISON ROBERT D ,  KLONOSKI JOSHUA

IPC: G01N33/548 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N33/549

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for an analyte, using an amplification polymer that multivalently binds to one or more non-analyte-specific binding site of the multivalent bridge conjugate, if present on the solid support.

Abstract translation: 公开的方法和材料涉及用于分析物诊断测定中扩增信号的改进方法，使用多价结合多价桥缀合物的一个或多个非分析物特异性结合位点的扩增聚合物（如果存在于 坚实的支持。

公开(公告)号：WO2009070640A3

公开(公告)日：2009-09-03

申请号：PCT/US2008084790

申请日：2008-11-26

Applicant: GREAT BASIN SCIENT ,  JENISON ROBERT D ,  KLONOSKI JOSHUA ,  TORRES ANTHONY R

Inventor： JENISON ROBERT D ,  KLONOSKI JOSHUA ,  TORRES ANTHONY R

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.

Abstract translation: 公开了关于用于扩增核酸诊断测定中的信号的改进方法的方法和材料，其包括提供与核酸分析物结合的扩增聚合物的步骤，其中所述扩增聚合物包含多个胺基团; 在扩增聚合物上具有可检测标记复合物的结合胺基; 并在高盐条件下使乙酰化化合物与未被可检测标记物复合物结合的胺基反应。