公开(公告)号：WO2003027255A3

公开(公告)日：2003-04-03

申请号：PCT/US2002/030611

申请日：2002-09-27

Applicant: TIBOTEC PHARMACEUTICALS LTD. ,  XIE, Dong ,  ERICKSON, John, W. ,  GRULICH, Paul

Inventor： XIE, Dong ,  ERICKSON, John, W. ,  GRULICH, Paul

IPC: C07K14/16

Abstract: Methods of identifying a fusion inhibitor and inhibitors of gp41-mediated membrane fusion are disclosed. The methods comprise, for example, providing a first helical polypeptide comprising a sequence of IQN17 (SEQ ID NO: 1); providing a second helical polypeptide of 34 or less than 34 amino acids comprising the amino acid sequence W-X1-X2-W-X3-X4-X5-I, wherein X1, X2, X3, X4, and X5 are each independently chosen from any amino acid except proline; measuring, by capillary zone electrophoresis, the degree of complex formation between these peptides; and comparing the measured degree of complex formation to the degree in the presence of a test composition.

公开(公告)号：WO2001060839A3

公开(公告)日：2001-08-23

申请号：PCT/US2001/004547

申请日：2001-02-12

Applicant: THE UNITED STATES OF AMERICA, as represented by SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  XIE, Dong ,  GRULICH, Paul ,  ERICKSON, John

Inventor： XIE, Dong ,  GRULICH, Paul ,  ERICKSON, John

IPC: C07K1/113

Abstract: A simple and reproducible method for refolding endostatin is provided. Recombinant endostatin isolated from bacterial inclusion bodies of large scale cultures can be refolded using this method. Refolding is accomplished by contacting endostatin with cyclodextrin in an aqueous medium buffered at a neutral or acidic pH (preferably, a pH of about 4 to about 5). The refolded endostatin has similar biological activity, including anti-proliferation activity against stimulated endothelial cells in culture, as native endostatin.

公开(公告)号：WO03027255A2

公开(公告)日：2003-04-03

申请号：PCT/US0230611

申请日：2002-09-27

Applicant: TIBOTEC PHARM LTD ,  XIE DONG ,  ERICKSON JOHN W ,  GRULICH PAUL

Inventor： XIE DONG ,  ERICKSON JOHN W ,  GRULICH PAUL

IPC: A61K45/00 ,  A61P31/18 ,  C07K7/00 ,  C07K14/47 ,  G01N27/447 ,  G01N33/15 ,  G01N33/48 ,  G01N33/50 ,  G01N33/561 ,  G01N33/569 ,  G01N33/68 ,  G06F19/00 ,  C12N

CPC classification number: G01N33/56988 ,  G01N33/6803 ,  G01N2333/162 ,  G01N2500/02

Abstract: Methods of identifying a fusion inhibitor and inhibitors of gp41-mediated membrane fusion are disclosed. The methods comprise, for example, providing a first helical polypeptide comprising a sequence of IQN17 (SEQ ID NO: 1); providing a second helical polypeptide of 34 or less than 34 amino acids comprising the amino acid sequence W-X1-X2-W-X3-X4-X5-I, wherein X1, X2, X3, X4, and X5 are each independently chosen from any amino acid except proline; measuring, by capillary zone electrophoresis, the degree of complex formation between these peptides; and comparing the measured degree of complex formation to the degree in the presence of a test composition.

Abstract translation: 公开了鉴定融合抑制剂和gp41介导的膜融合抑制剂的方法。 所述方法包括例如提供包含IQN17（SEQ ID NO：1）序列的第一螺旋多肽; 提供包含氨基酸序列W-X1-X2-W-X3-X4-X5-I的34个或小于34个氨基酸的第二螺旋多肽，其中X1，X2，X3，X4和X5各自独立地选自 除脯氨酸以外的任何氨基酸; 通过毛细管区带电泳测量这些肽之间的复合物形成程度; 并将测量的复合物形成程度与存在测试组合物的程度进行比较。

公开(公告)号：WO0160839A9

公开(公告)日：2002-10-17

申请号：PCT/US0104547

申请日：2001-02-12

Applicant: US HEALTH ,  XIE DONG ,  GRULICH PAUL ,  ERICKSON JOHN

Inventor： XIE DONG ,  GRULICH PAUL ,  ERICKSON JOHN

IPC: C07K1/113 ,  C07K14/78

CPC classification number: C07K1/1136 ,  C07K14/78

Abstract: A simple and reproducible method for refolding endostatin is provided. Recombinant endostatin isolated from bacterial inclusion bodies of large scale cultures can be refolded using this method. Refolding is accomplished by contacting endostatin with cyclodextrin in an aqueous medium buffered at a neutral or acidic pH (preferably, a pH of about 4 to about 5). The refolded endostatin has similar biological activity, including anti-proliferation activity against stimulated endothelial cells in culture, as native endostatin.

Abstract translation: 提供了用于重新折叠内皮抑素的简单且可重现的方法。 从大规模培养物的细菌包涵体分离的重组内皮抑素可以使用这种方法再折叠。 通过使内皮抑素与环糊精在中性或酸性pH（优选约4至约5的pH）缓冲的水性介质中接触来完成重折叠。 重折叠的内皮抑素具有相似的生物学活性，包括对培养物中刺激的内皮细胞的抗增殖活性，如天然内皮抑素。

公开(公告)号：WO0160839A2

公开(公告)日：2001-08-23

申请号：PCT/US0104547

申请日：2001-02-12

Applicant: US HEALTH ,  XIE DONG ,  GRULICH PAUL ,  ERICKSON JOHN

Inventor： XIE DONG ,  GRULICH PAUL ,  ERICKSON JOHN

IPC: C07K1/113 ,  C07K14/78 ,  C07K1/00

CPC classification number: C07K1/1136 ,  C07K14/78

Abstract: A simple and reproducible method for refolding endostatin is provided. Recombinant endostatin isolated from bacterial inclusion bodies of large scale cultures can be refolded using this method. Refolding is accomplished by contacting endostatin with cyclodextrin in an aqueous medium buffered at a neutral or acidic pH (preferably, a pH of about 4 to about 5). The refolded endostatin has similar biological activity, including anti-proliferation activity against stimulated endothelial cells in culture, as native endostatin.

Abstract translation: 提供了一种用于重折叠内皮抑素的简单且可重现的方法。 用大规模培养物从细菌包涵体分离的重组内皮抑制素可以用这种方法重折叠。 通过使内皮抑制素与环糊精在中性或酸性pH（优选约4至约5的pH）缓冲的水性介质中接触来实现再折叠。 重折叠的内皮抑制素具有类似的生物学活性，包括对培养物中刺激的内皮细胞的抗增殖活性，作为天然内皮抑制素。