公开(公告)号：WO1991010890A1

公开(公告)日：1991-07-25

申请号：PCT/EP1991000063

申请日：1991-01-16

Applicant: SITTE, Hellmuth ,  HÄSSIG, Helmut ,  NEUMANN, Klaus

IPC: G01N01/06

CPC classification number: G01N1/06

Abstract: An ultramicrotome consists of an aluminium base (1'), a bearing block (4) with the bearings (5, 7) for an intermediate lever (6) and a preparation rod (8) with preparation (9), and a support (2, 21) for the blade (3). The micrometer spindle (19') with the spindle nut (20) is prolonged by an extension rod (25, 25', 35) made of a material with a low coefficient of thermal expansion arranged below the preparation support rod (8) and connected directly or via intermediate elements with the aluminium base (1') of the device. At least one bearing element (26, 31, 33) allows the micrometer spindle (19') to be rotated about its longitudinal axis (22/22') and is held without play by springs (27). Embodiments of the invention provide a thermal insulation (37-40), and thermostatic heating of the aluminium base (1') and of the essential mechanical elements of the ultramicrotome to a temperature slightly above ambient temperature.

Abstract translation: 超薄切片机由铝基座（1'），具有用于中间杆（6）的轴承（5,7）的轴承座（4）和具有制备（9）的准备棒（8）和支撑件 2），用于刀片（3）。 具有主轴螺母（20）的千分尺主轴（19'）被延伸杆（25,25'，35）延长，所述延伸杆（25,25'，35）由布置在制备支撑杆（8）下方的具有低热膨胀系数的材料制成并连接 直接或经由与装置的铝基（1'）的中间元件。 至少一个轴承元件（26,33,33）允许千分尺主轴（19'）围绕其纵向轴线（22/22'）旋转并且被弹簧（27）保持而不起作用。 本发明的实施例提供了绝热（37-40），并且将铝基底（1'）和超薄切片机的基本机械元件的恒温加热到略高于环境温度的温度。

公开(公告)号：WO1994005995A1

公开(公告)日：1994-03-17

申请号：PCT/EP1993002408

申请日：1993-09-06

Applicant: LEICA AG ,  SITTE, Hellmuth ,  NEUMANN, Klaus ,  EDELMANN, Ludwig ,  HÄSSIG, Helmut ,  LANG, Anton ,  KLEBER, Heinrich

Inventor： LEICA AG

IPC: G01N01/28

CPC classification number: F17C3/085 ,  F17C2203/03 ,  F17C2203/0636 ,  F17C2221/014 ,  F17C2223/0161 ,  F25D3/10 ,  G01N1/36 ,  G01N1/42

Abstract: A device for dehydrating and/or embedding preferably frozen samples, consisting of a dewar vessel (1) filled with liquid nitrogen (2) and a metal component (4) of a good heat conducting material secured to the base, the upper end of which in the base region of the dewar vessel neck has a cover (5) with a metal cooling surface (6, 7) which corresponds to matching lower contact surfaces of the thermostatically heated (9, 10) substitution (PLT) container (8) or the lower part of a freeze-drying chamber in such a way as to ensure good heat transfer between the corresponding surfaces.

公开(公告)号：WO1991015746A1

公开(公告)日：1991-10-17

申请号：PCT/AT1991000046

申请日：1991-03-18

Applicant: SITTE, Hellmuth ,  HÄSSIG, Helmut ,  KUNZ, Armin ,  NEUMANN, Klaus

IPC: G01N01/06

CPC classification number: G01N1/06 ,  G01N2001/066

Abstract: Proposed is a microtome, in particular an ultramicrotome, with an electrical drive (19) for the relative motion of the blade (9) and the specimen (7) and with an electronic control device (20) which controls the drive (19) to change the drive speed. In the cutting zone (S2) in which slice removal takes place, the cutting speed is lower. In order, after slice removal, to bring the moving part, e.g. the blade or the specimen, back into the position it must occupy for the next cutting stroke, a higher speed is called for. In order to optimise the cutting process and thus, if required, to reduce the cycle time, the invention calls for the profile of the relative speed between specimen (7) and blade (9) (cutting speed) within the cutting zone (S2) to differ from a constant-speed profile.

Abstract translation: 提出了一种切片机，特别是超薄切片机，具有用于叶片（9）和样品（7）的相对运动的电驱动器（19）和用于控制驱动器（19）的电子控制装置（20） 改变驱动速度。 在进行切片移除的切割区域（S2）中，切割速度较低。 为了在切片移除之后，带动移动部分，例如， 刀片或样品回到必须占据下一个切割行程的位置，需要更高的速度。 为了优化切割过程，因此如果需要，为了减少循环时间，本发明要求在切割区域（S2）内的样品（7）和叶片（9）之间的相对速度（切割速度）的轮廓， 与恒速曲线不同。

公开(公告)号：WO1988002851A2

公开(公告)日：1988-04-21

申请号：PCT/EP1987000596

申请日：1987-10-13

Applicant: SITTE, Hellmuth ,  HÄSSIG, Helmut ,  NEUMANN, Klaus

IPC: G01N01/06

CPC classification number: F25D3/10 ,  G01N1/06 ,  G01N1/42

Abstract: A refrigerated chamber comprises, inside its metal wall (24/24') and a foam insulating layer (39), a sheet metal tank (38'/40'), in which, depending on the level of filling with liquid nitrogen, the various compartments of the tank divided by separations (72a/72a', 81/81') of different height are filled with LN2, so that different cooling powers are available for the varying temperature ranges between the ambient temperature and the temperature of the liquid nitrogen; in this way one avoids an excessive consumption of nitrogen. The filling with LN2 is effected from a separating tank (38'/40'/75/75') by means of a horizontal small-diameter pipe (73), which during operation of the chamber, is always exposed to and cooled by liquid nitrogen. The nitrogen in the form of a sweeping gas emerging in a boiling state from the tank (38'/40') and guided through the cutting space (41') can be heated up by a heating plate (67'/69') fixed to the sheet metal deflectors (48/48'). Arrangements relate inter alia to a cooled receiving surface (85) and a simplified chamber assembly having a high degree of stability (89 to 98).

Abstract translation: 冷藏室在其金属壁（24/24'）和泡沫绝缘层（39）的内部包括金属板（38'/ 40'），其中，根据液氮的填充水平， 通过不同高度的分离（72a / 72a'，81/81'）分隔的罐的各个隔室填充有LN2，使得不同的冷却功率可用于环境温度和液氮温度之间的变化的温度范围 ; 以这种方式避免了氮的过量消耗。 LN2的填充通过水平小直径管道（73）从分离罐（38'/ 40'/ 75/75'）进行，在管腔运行期间，总是暴露于液体并被液体冷却 氮。 从罐（38'/ 40'）出来并被引导通过切割空间（41'）的沸腾状态的清扫气体形式的氮气可以通过加热板（67'/ 69'）被固定 到金属板偏转器（48/48'）。 布置尤其涉及冷却的接收表面（85）和具有高度稳定性（89至98）的简化室组件。

公开(公告)号：WO1994005996A1

公开(公告)日：1994-03-17

申请号：PCT/EP1993002409

申请日：1993-09-06

Applicant: LEICA AG ,  SITTE, Helmuth ,  NEUMANN, Klaus ,  EDELMANN, Ludwig ,  HÄSSIG, Helmut ,  KLEBER, Heinrich

Inventor： LEICA AG

IPC: G01N01/28

CPC classification number: F17C3/085 ,  F25D3/10 ,  F26B5/06 ,  G01N1/42

Abstract: A chamber for freeze-drying by cryosorption with liquid nitrogen cooling in a Dewar vessel (1), in which the chamber (8-10, 18, 19) is largely in the Dewar vessel (1), preferably in its neck, and its preferably cylindrical outer wall (9) is of metal, the lower edge of which is vacuumtightly secured to lower edge of a metal and preferably symmetrical in rotation component (8). The plane underside of the metal component (8) has a contact surface (25) which corresponds to the equally plane complementary surface (7) on the top of a body around which flows liquid nitrogen and is cooled by this component, the component (8) having a chamber (30) to accept the dessiccant (31) for cryosorption, e.g. a molecular sieve, and a connection (45) with the drying chamber (34). The upper edge of the metal wall (9) or a ring (10) vacuumtightly secured thereto is outside the Dewar vessel (1) and is therefore at approximately room temperature and has a vacuum connector (14) for the connection of commercially available vacuum components, and the aperture on the upper side of the ring (10) can be vacuumtightly sealed preferably by a commercially available O-ring together with a cover (19).

公开(公告)号：WO1991003679A1

公开(公告)日：1991-03-21

申请号：PCT/EP1990001484

申请日：1990-09-05

Applicant: SITTE, Hellmuth ,  HÄSSIG, Helmut ,  KUNZ, Armin ,  NEUMANN, Klaus

IPC: F17C13/00

CPC classification number: F17C9/00 ,  F17C6/00 ,  F17C13/021 ,  F17C13/025 ,  F17C2221/014 ,  F17C2223/0153 ,  F17C2225/047 ,  F17C2250/0408 ,  F17C2250/061 ,  G01N1/06 ,  G01N1/42 ,  G05D9/12

Abstract: A device for continuously refilling a cold chamber with liquid nitrogen consists of a Dewar flask (1) filled with liquid nitrogen (2), which has a stopper (13, 14) and a filling tube (6) which passes through the stopper (13) into the cold chamber tank (7). Overpressure ensures a continuous flow of liquid nitrogen (2) against the hydrostatic pressure of the column of nitrogen. An electronic unit (15) connected to a heating cartridge (8) controls the flow rate of the liquid nitrogen in function of the level gauges of the sensors (16, 17) and of the pressure gauge of the sensor (18) so that the level of the liquid nitrogen in the cold member tank (7) is maintained at a constant height (h). Equilibrium of flow is thus maintained between the supply of liquid nitrogen to, and the evaporation of liquid nitrogen in the colder chamber (7).

Abstract translation: 用于用液氮连续重新填充冷室的装置由填充有液氮（2）的杜瓦瓶（1）组成，其具有通过塞子（13）的塞子（13,14）和填充管（6） ）进入冷室容器（7）。 超压确保液氮（2）与氮柱的静水压力持续流动。 连接到加热盒（8）的电子单元（15）根据传感器（16,17）的电平计和传感器（18）的压力计来控制液氮的流量，使得 冷组件箱（7）中液氮的水平保持恒定的高度（h）。 因此，在冷却室（7）中的液氮供应和液氮蒸发之间保持平衡的流动。