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    VERWENDUNG VON FMN-BINDENDEN FLUORESZENZPROTEINEN (FBFP) ALS NEUARTIGE SEKRETIONSMARKER
    4.
    发明申请
    VERWENDUNG VON FMN-BINDENDEN FLUORESZENZPROTEINEN (FBFP) ALS NEUARTIGE SEKRETIONSMARKER 审中-公开
    FMN结合蛋白荧光作为新颖分泌标记USE（FBFP）

    公开(公告)号：WO2013020831A1

    公开(公告)日：2013-02-14

    申请号：PCT/EP2012/064776

    申请日：2012-07-27

    Applicant: EVOCATAL GMBH ,  DREPPER, Thomas ,  JAEGER, Karl-Erich ,  POTZKEI, Janko

    Inventor： DREPPER, Thomas ,  JAEGER, Karl-Erich ,  POTZKEI, Janko

    IPC: C07K14/195

    CPC classification number: G01N33/52 ,  C07K14/195 ,  C07K14/245 ,  C12Q1/68

    Abstract: Zur Untersuchung von Protein-Protein Interaktionen, Proteinfaltungen und Proteinlokalisation sowie bei der Sekretion von Proteinen werden in der Biotechnologie und in der Grundlagenforschung in vivo Reporterproteine eingesetzt. Um Fluoreszenzreporter als Marker für Sekretionsprozesse nutzen zu können, wurden von uns erstmals FMN-bindende Fluoreszenproteine (FbFP) entwickelt. Die neuen Fluoreszenzmarker lassen sich wie GFP in verschiedenen Bakterien exprimieren. Durch die Bindung des Chromophors FMN entsteht so ein cyan-grün fluoreszierendes Protein, das sich in vivo mit allen gängigen spektroskopischen und mikroskopischen Verfahren nachweisen lässt. Im Gegensatz zu GFP kann dieses Protein überraschender Weise auch über den Sec-Weg sekretiert und im Periplasma in den fluoreszenzaktiven Zustand überführt werden.

    Abstract translation: 对于蛋白质 - 蛋白质相互作用，蛋白质折叠和蛋白定位的调查以及蛋白质的分泌体内报道蛋白的生物技术和基础研究使用。 使用荧光报告作为分泌过程的标志，我们首次FMN结合Fluoreszenproteine（FbFP）开发。 新的荧光标记物可以在不同的细菌，如GFP表达。 由发色团FMN结果的结合是青色的绿色荧光蛋白可在体内与所有标准光谱和显微技术来检测。 相反GFP这种蛋白质，出人意料的是，也可以通过Sec途径分泌，并在周质中被转化成荧光激活状态。

    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    6.
    发明申请
    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
    一个生产精细和特种化学品的新一批产品

    公开(公告)号：WO2007128441A3

    公开(公告)日：2008-02-28

    申请号：PCT/EP2007003772

    申请日：2007-04-27

    Applicant: B R A I N AG ,  ELEND CHRISTIAN ,  JAEGER KARL-ERICH ,  LEGGEWIE CHRISTIAN ,  SCHMEISSER CHRISTEL ,  STREIT WOLFGANG

    Inventor： ELEND CHRISTIAN ,  JAEGER KARL-ERICH ,  LEGGEWIE CHRISTIAN ,  SCHMEISSER CHRISTEL ,  STREIT WOLFGANG

    IPC: C12N9/18

    CPC classification number: C12N9/18

    Abstract: The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.

    Abstract translation: 本发明涉及编码具有羧基酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6和8中任一个所示氨基酸序列的多核苷酸; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5和7中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代 （a）的顺序; （d）编码具有羧基酯酶活性的酶的多核苷酸与编码如SEQ ID NO：2,4,6和8之一所示的酶的多核苷酸至少65％相同; （e）具有或包含核苷酸序列的多核苷酸，其互补链与（a）至（d）中任一项所定义的多核苷酸杂交; 和（T）具有或包含与（d）或（e）的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或（a）至（f）的这种多核苷酸或其片段的互补链用作特异性探针或引物。 本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。 本发明还涉及包含由本发明的多核苷酸编码或可通过本发明的方法获得的氨基酸序列的多肽。 此外，本发明涉及生产所述多肽和产生表达所述多肽的细菌的方法。 最后，本发明涉及包含本发明的多核苷酸，本发明的载体，本发明的宿主，本发明的多肽，本发明的抗体和/或一种或多种的组合物 本发明的引物。

    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    7.
    发明申请
    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
    一个生产精细和特种化学品的新一批产品

    公开(公告)号：WO2007128441A2

    公开(公告)日：2007-11-15

    申请号：PCT/EP2007/003772

    申请日：2007-04-27

    Applicant: B.R.A.I.N. AG ,  ELEND, Christian ,  JAEGER, Karl-Erich ,  LEGGEWIE, Christian ,  SCHMEISSER, Christel ,  STREIT, Wolfgang

    Inventor： ELEND, Christian ,  JAEGER, Karl-Erich ,  LEGGEWIE, Christian ,  SCHMEISSER, Christel ,  STREIT, Wolfgang

    IPC: C12N9/18

    CPC classification number: C12N9/18

    Abstract: The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.

    Abstract translation: 本发明涉及编码具有羧基酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6和8中任一个所示氨基酸序列的多核苷酸; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5和7中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代 （a）的顺序; （d）编码具有羧基酯酶活性的酶的多核苷酸与编码如SEQ ID NO：2,4,6和8之一所示的酶的多核苷酸至少65％相同; （e）具有或包含核苷酸序列的多核苷酸，其互补链与（a）至（d）中任一项所定义的多核苷酸杂交; 和（T）具有或包含与（d）或（e）的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或（a）至（f）的这种多核苷酸或其片段的互补链用作特异性探针或引物。 本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。 本发明还涉及包含由本发明的多核苷酸编码或可通过本发明的方法获得的氨基酸序列的多肽。 此外，本发明涉及生产所述多肽和产生表达所述多肽的细菌的方法。 最后，本发明涉及包含本发明的多核苷酸，本发明的载体，本发明的宿主，本发明的多肽，本发明的抗体和/或一种或多种的组合物 本发明的引物。

    LIPASE PRODUCTION METHOD
    8.
    发明申请
    LIPASE PRODUCTION METHOD 审中-公开
    用于生产脂肪酶

    公开(公告)号：WO2006133900A9

    公开(公告)日：2007-03-08

    申请号：PCT/EP2006005668

    申请日：2006-06-13

    Applicant: BASF AG ,  BESELIN ANKE ,  ROSENAU FRANK ,  JAEGER KARL-ERICH ,  BREUER MICHAEL ,  HAUER BERNHARD

    Inventor： BESELIN ANKE ,  ROSENAU FRANK ,  JAEGER KARL-ERICH ,  BREUER MICHAEL ,  HAUER BERNHARD

    IPC: C12N9/52

    CPC classification number: C12N9/52 ,  C12N9/20

    Abstract: The invention relates to novel proteins, in particular, exhibiting a protease activity and promoting the production of extracellular lipases by bacteria, in particular, by Burkholderia-type bacteria, to coding nucleic acid sequences, expression constructs containing them, host modified thereby, to a method for producing proteins exhibiting a protease activity and to a lipase production method, in particular by Burkholderia-type bacteria.

    Abstract translation: 本发明涉及的特别是伯克霍尔德氏菌属，含有这些表达构建体的核酸编码的序列，宿主经转化与之新的蛋白质，特别是具有蛋白酶活性，其通过细菌促进生产细胞外脂肪酶的，用于制备具有蛋白酶活性以及方法的蛋白质的用于生产脂酶的方法， 特别是通过伯克霍尔德氏菌属的细菌。

    USE OF ESTERASES FOR SEPARATING PLASTICS
    9.
    发明申请
    USE OF ESTERASES FOR SEPARATING PLASTICS 审中-公开
    使用酯类物质切割塑料

    公开(公告)号：WO2007017181A3

    公开(公告)日：2007-08-02

    申请号：PCT/EP2006007693

    申请日：2006-08-03

    Applicant: HENKEL KGAA ,  MICHELS ANDREAS ,  PUETZ ANDRE ,  MAURER KARL-HEINZ ,  EGGERT THORSTEN ,  JAEGER KARL-ERICH

    Inventor： MICHELS ANDREAS ,  PUETZ ANDRE ,  MAURER KARL-HEINZ ,  EGGERT THORSTEN ,  JAEGER KARL-ERICH

    IPC: C12N9/18 ,  C12N5/10 ,  C12N15/63

    CPC classification number: C12N9/18

    Abstract: The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof.

    Abstract translation: 本发明涉及含有酯酶剂，以及涉及它们用于纤维的整理，特别是人造纤维，洗涤和含有酯酶和相应的洗涤和清洁方法和其他技术应用的清洁剂的用途。 更具体地说，它涉及使用酯酶的用于防止或减少或防止起球，优选在纺织品，特别是塑料纤维，特别优选聚酯纤维，以及用于塑料裂解使用酯酶，特别是聚酯化合物。 它还涉及新的酯酶和足够相关的蛋白质及其衍生物，含有它们的试剂及其用途。

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