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    METHOD FOR PROFILING PHYTOHORMONE LEVELS IN PLANT TISSUE
    8.
    发明申请
    METHOD FOR PROFILING PHYTOHORMONE LEVELS IN PLANT TISSUE 审中-公开
    在植物组织中分析植物酮水平的方法

    公开(公告)号：WO2012140603A1

    公开(公告)日：2012-10-18

    申请号：PCT/IB2012/051818

    申请日：2012-04-13

    Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  BASF (CHINA) COMPANY LIMITED ,  FUCHS, Regine ,  DOSTLER, Martin ,  WAHL, Maria ,  HENTSCHEL, Benjamin ,  LOOSER, Ralf

    Inventor： FUCHS, Regine ,  DOSTLER, Martin ,  WAHL, Maria ,  HENTSCHEL, Benjamin ,  LOOSER, Ralf

    IPC: G01N30/62 ,  G01N30/60 ,  A01N65/00

    CPC classification number: G01N33/74 ,  G01N30/06 ,  G01N30/7233 ,  G01N30/7266 ,  G01N2030/009 ,  G01N2030/062 ,  G01N2030/8813 ,  Y10T436/141111 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/255

    Abstract: The present invention provides a method for profiling phytohormone levels in plant tissue or tissue of other plastid containing organisms, i.e. a method for the simultaneous determination of a multitude of phytohormone levels in plant tissue or tissue of other plastid containing organisms, which method comprises the following steps: i. extraction of particulate tissue material of the plastid containing organism, in particulate tissue material of plants, with a liquid extractant, which is a mixture of at least one water miscible neutral organic solvent having from 1 to 3 carbon atoms and 1 heteroatom selected from O and N with water containing from 0.1 to 5% by weight, based on the extractant, of at least one acid, whereby a first liquid extract is obtained; ii. contacting the liquid extract obtained in step i. with a solid absorbent having a hydrophobically modified surface and removing the solid absorbent to obtain a second liquid extract; iii. evaporating the solvent from the second extract and then re-dissolving the obtained residue in a solvent mixture of at least one water miscible neutral organic solvent having from 1 to 3 carbon atoms and 1 heteroatom selected from O and N with water containing from 0.1 to 5% by weight, based on the solvent mixture, of at least one acid to obtain a reconstituted extract; iv. purification of the reconstituted extract by contacting it with a solid absorbent having a hydrophobically modified surface to obtain a purified reconstituted extract (eluate); and v. determining the relative concentrations of at least two, frequently at least 4, in particular at least 6, especially at least 8 or at least 10 phyto, e.g. from 2 to 60, frequently from 4 to 50, in particular from 6 to 45, especially from 8 to 40 or from 10 to 30 plant hormones (phytohormones) in the purified reconstituted extract obtained in step iv. by directly subjecting the purified reconstituted extract to an analyzing unit comprising a separation unit for separation the phytohormones and an analyzer for identifying the phytohormones which separation unit is coupled to the analyzer.

    Abstract translation: 本发明提供了用于在植物组织或其他含有质体的生物体的组织中分析植物激素水平的方法，即用于同时测定植物组织或其他含有物质的生物体的组织中的大量植物激素水平的方法，该方法包括以下 步骤：我 在植物的颗粒组织材料中用液体萃取剂萃取含有质体的生物体的颗粒组织材料，其是至少一种具有1至3个碳原子的水混溶性中性有机溶剂和1个选自O和O的杂原子的混合物， N，其中含有0.1至5重量％的基于萃取剂的至少一种酸，由此获得第一液体提取物; II。 使步骤i中获得的液体提取物接触。 具有疏水改性表面的固体吸收剂并除去固体吸收剂以获得第二液体提取物; III。 从第二提取物蒸发溶剂，然后将得到的残余物重新溶解在至少一种具有1至3个碳原子的水混溶性中性有机溶剂和1个选自O和N的杂原子的溶剂混合物中，所述溶剂混合物含有0.1至5 基于溶剂混合物的至少一种酸重量以获得重构的提取物; IV。 通过使其与具有疏水改性表面的固体吸收剂接触来纯化重构的提取物，以获得纯化的重构提取物（洗脱液）; 和v。确定至少两个，经常至少4个，特别是至少6个，特别是至少8个或至少10个植物的相对浓度。 在步骤iv中获得的纯化的再提取的提取物中，从2至60，通常为4至50，特别是6至45，特别是8至40或10至30种植物激素（植物激素）。 通过将纯化的重构提取物直接经受包含用于分离植物激素的分离单元的分析单元和用于鉴定分离单元与分析仪结合的植物激素的分析器。

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