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    OPTICAL PAD AND SYSTEM EMPLOYING THE SAME
    2.
    发明申请
    OPTICAL PAD AND SYSTEM EMPLOYING THE SAME 审中-公开

    公开(公告)号：WO2021250013A1

    公开(公告)日：2021-12-16

    申请号：PCT/EP2021/065320

    申请日：2021-06-08

    申请人： JOHANN WOLFGANG GOETHE-UNIVERSITÄT FRANKFURT AM MAIN ,  INSTITUTE FOR RESEARCH IN BIOMEDICINE (IRB BARCELONA)

    发明人： PAMPALONI, Francesco ,  COLOMBELLI, Julien

    IPC分类号： G02B21/33 ,  G02B21/00 ,  G02B21/34 ,  G02B21/0088

    摘要： Disclosed herein is an optical system comprising a microscope objective lens (10), a sample holder (12), and an optical pad (20) to be provided in a gap between said microscope objective lens (10) and said sample holder (12). The optical pad (20) is attached or attachable to one of the microscope objective lens (10) and the sample holder (12) and has a dry, free surface (22) configured to be in contact with an abutment surface (24) provided on the other of the microscope objective lens (10) and the sample holder (12) during microscopy, but free to move with respect to it to allow for carrying out microscopy at different relative positions between said free surface (22) and said abutment surface (24). The pad (20) is deformable such that the relative distance between said microscope objective lens (10) and said sample holder (12) can be varied by a working distance while continuously filling the gap between said microscope objective lens (10) and said sample holder (12).

    METHOD FOR GENERATING HIGHER ORDER GENOME EDITING LIBRARIES
    6.
    发明申请
    METHOD FOR GENERATING HIGHER ORDER GENOME EDITING LIBRARIES 审中-公开

    公开(公告)号：WO2018122248A1

    公开(公告)日：2018-07-05

    申请号：PCT/EP2017/084625

    申请日：2017-12-27

    申请人： JOHANN WOLFGANG GOETHE-UNIVERSITÄT FRANKFURT AM MAIN

    发明人： KAULICH, Manuel ,  ERNST, Andreas ,  WEGNER, Martin ,  DIEHL, Valentina ,  DE BRUYN, Rahel ,  WIECHMANN, Svenja

    IPC分类号： C12N15/10

    摘要： The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.

    MICROBIOLOGICAL PRODUCTION OF SHORT FATTY ACIDS AND USES THEREOF
    7.
    发明申请
    MICROBIOLOGICAL PRODUCTION OF SHORT FATTY ACIDS AND USES THEREOF 审中-公开
    微生物生产短脂肪酸及其用途

    公开(公告)号：WO2016156548A1

    公开(公告)日：2016-10-06

    申请号：PCT/EP2016/057167

    申请日：2016-03-31

    申请人： JOHANN WOLFGANG GOETHE-UNIVERSITÄT FRANKFURT AM MAIN

    发明人： GRININGER, Martin ,  GAJEWSKI, Jan ,  BOLES, Eckard ,  PAVLOVIC, Renata

    IPC分类号： C12N9/10 ,  C12N15/82

    CPC分类号： C12N9/1029 ,  C12N15/82 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12P7/649 ,  Y02E50/13

    摘要： The present invention relates to proteins involved in fatty acid synthesis, such as fatty acid synthases (FAS) variants, comprising one or more polypeptide chains, wherein said polypeptide chain(s) comprise one or more subunits comprising a malonyl/palmitoyl transferase domain (MPT domain), acetyl transferase domain (AT domain), and ketoacyl synthase domain (KS domain), and at least one amino acid substitution in the MPT domain at a position corresponding to R130, in the AT domain at a position corresponding to I306, and/or in the KS domain, preferably in the acyl binding channel and/or at KS domain binding site to ACP, to modulate affinities of acyl intermediates, and optionally further amino acid substitution(s). The present invention relates to the respective polypeptide domains. The present invention further relates to nucleic acid molecules encoding the proteins (or the polypeptide domains) and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of short fatty acids, CoA esters of short fatty acids, ethyl esters of short fatty acids, esters of short fatty acids with other metabolites, and/or enzyme bound short fatty acids (C 6 to C 12 ), comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention further relates to a method for the production of biofuels, flavoring compounds and/or fine chemicals, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the proteins, nucleic acids molecule or host cells for the bulk production of short fatty acids (C 6 to C 12 ), the specific production of C 6 fatty acids and/or C8 fatty acids, the bulk production of CoA esters of short fatty acids (C 6 to C 12 ), the specific production of C 6 -CoA esters and/or C 8 -CoA esters, the bulk production of ethyl esters of short fatty acids (C 6 to C 12 ), the specific production of C 6 fatty acid ethyl esters and/or C 8 fatty acid ethyl esters, the bulk production of esters of short fatty acids (C 6 to C 12 ) with other metabolites, the specific production of C 6 fatty acid esters with other metabolites and/or C 8 fatty acid esters with other metabolites, the bulk production of enzyme bound short fatty acids (C 6 to C 12 ), the specific production of enzyme bound C 6 fatty acids and/or enzyme bound C 8 fatty acids, the production of biofuels, fine chemicals and/or flavoring substances.

    摘要翻译： 本发明涉及脂肪酸合成中涉及的蛋白质，例如包含一个或多个多肽链的脂肪酸合成酶（FAS）变体，其中所述多肽链包含一个或多个包含丙二酰/棕榈酰转移酶结构域（MPT）的亚基 结构域），乙酰转移酶结构域（AT结构域）和酮酰基合成酶结构域（KS结构域），以及在对应于I306的位置的AT结构域中，在对应于R130的位置的MPT结构域中至少一个氨基酸取代，以及 /或在KS结构域中，优选在酰基结合通道中和/或在KS结构域与ACP结合，调节酰基中间体的亲和力，以及任选地进一步的氨基酸取代。 本发明涉及相应的多肽结构域。 本发明还涉及编码蛋白质（或多肽结构域）的核酸分子以及含有所述核酸分子的宿主细胞。 本发明还涉及一种生产短脂肪酸的方法，短脂肪酸的辅酶A，短脂肪酸的乙酯，短脂肪酸与其它代谢物的酯，和/或酶结合的短脂肪酸（C6至 C12），包括所述核酸分子的表达，优选在所述宿主细胞中。 本发明还涉及一种生产生物燃料，调味化合物和/或精细化学品的方法，其包括优选在所述宿主细胞中表达所述核酸分子。 本发明还涉及蛋白质，核酸分子或宿主细胞用于大量生产短脂肪酸（C6至C12），C6脂肪酸和/或C8脂肪酸的特异性生产，批量生产 短链脂肪酸（C6〜C12）的CoA酯，C6-CoA酯和/或C8-CoA酯的特异性生产，大量生产短脂肪酸（C6〜C12）的乙酯，C6脂肪酸的特异性生产 酸性乙酯和/或C8脂肪酸乙酯，大量生产短脂肪酸（C6至C12）与其他代谢物的酯类，与其他代谢物和/或C8脂肪酸酯与其他代谢产物特异性生产C6脂肪酸酯 代谢物，大量生产酶结合的短脂肪酸（C6〜C12），具体生产酶结合的C6脂肪酸和/或酶结合的C8脂肪酸，生产生物燃料，精细化学品和/或调味物质。

    GENERATION OF A MESENCHYMAL STROMAL CELL BANK FROM THE POOLED MONONUCLEAR CELLS OF MULTIPLE BONE MARROW DONORS
    8.
    发明申请
    GENERATION OF A MESENCHYMAL STROMAL CELL BANK FROM THE POOLED MONONUCLEAR CELLS OF MULTIPLE BONE MARROW DONORS 审中-公开
    来自多个骨髓基质细胞的细胞单核细胞的骨髓瘤细胞库的产生

    公开(公告)号：WO2016008895A1

    公开(公告)日：2016-01-21

    申请号：PCT/EP2015/066083

    申请日：2015-07-14

    申请人： JOHANN WOLFGANG GOETHE-UNIVERSITÄT, FRANKFURT AM MAIN ,  DRK BLUTSPENDEDIENST BADEN-WÜRTTEMBERG-HESSEN GGMBH

    发明人： BADER, Peter ,  KUCI, Selim ,  KUCI, Zyrafete ,  BÖNIG, Halvard

    IPC分类号： C12N5/0775

    CPC分类号： C12N5/0663 ,  A61K35/28 ,  A61K2035/124 ,  C12Q1/6881

    摘要： The present invention pertains to an improved mesenchymal stromal cell (MSC) preparation and a method for producing the same. The invention provides a new strategy to isolate MSC from bone marrow mononuclear cells (BM-MNCs) by pooling BM-MNCs of multiple unrelated (third-party) bone marrow donors. The MSC preparation manufactured in accordance with the methodology of the invention is characterized by a stable proliferative capability and an increased immunosuppressive potential when compared to individual donor MSC preparations or a pool of individual MSCs generated from multiple donors. The MSCs prepared ac- cording to the invention are particularly useful for medical applications such as the treatment of graft-versus-host disesase (GvHD) in recipients with hematopoietic stem cell transplants, patients with autoimmune disorders and as a cell-based therapy in regenerative medicine.

    摘要翻译： 本发明涉及一种改良的间充质基质细胞（MSC）制剂及其制备方法。 本发明提供了通过汇集多个不相关（第三方）骨髓供体的BM-MNCs来分离MSC与骨髓单核细胞（BM-MNC）的新策略。 根据本发明的方法制备的MSC制剂的特征在于与单个供体MSC制剂或由多个供体产生的各个MSC的池相比时，具有稳定的增殖能力和增加的免疫抑制潜力。 根据本发明制备的MSC对于医学应用特别有用，例如在造血干细胞移植受者，自身免疫性疾病患者和再生中的基于细胞的治疗的接受者中对移植物抗宿主病（GvHD）的治疗 医学。

    DIALYSEZELLE FÜR EINE IN-VITRO-FREISETZUNGSTESTAPPARATUR, VERWENDUNG DER DIALYSEZELLE UND IN-VITRO-FREISETZUNGSTESTAPPARATUR
    9.
    发明申请
    DIALYSEZELLE FÜR EINE IN-VITRO-FREISETZUNGSTESTAPPARATUR, VERWENDUNG DER DIALYSEZELLE UND IN-VITRO-FREISETZUNGSTESTAPPARATUR 审中-公开
    透析小区生物体外释放试验设备，采用渗析池和生物体外释放试验设备

    公开(公告)号：WO2015039749A1

    公开(公告)日：2015-03-26

    申请号：PCT/EP2014/002523

    申请日：2014-09-17

    申请人： JOHANN WOLFGANG GOETHE-UNIVERSITÄT FRANKFURT AM MAIN

    发明人： WACKER, Matthias ,  JANAS, Christine

    IPC分类号： G01N33/15 ,  B01D61/24 ,  B01D63/06 ,  G01N13/00

    CPC分类号： G01N33/15 ,  B01D61/28 ,  B01D2313/06 ,  G01N2013/006

    摘要： Bei einer Dialysezelle für eine In-vitro-Freisetzungstestapparatur zum Ermitteln eines Freisetzungsverhaltens, wie einer Freisetzungsrate, einer Substanz einer Stoffzusammensetzung, wie eines Arzneimittels, welche Substanz an Molekularträger, wie Liposome, Mizellen oder Nanopartikel, gebunden ist, gegenüber einem Substanzaufnahmefluid mit einem Zelleninnenraum und einer den Zelleninnenraum zumindest teilweise begrenzende Dialysemembran, die die gebundene Substanz zurückhält, für in das Substanzaufnahmefluid freigesetzte Substanz durchlässig ist und für das Substanzaufnahmefluid zumindest teilweise durchlässig ist, ist vorgesehen, dass die Dialysemembran an einer Tragstruktur befestigt ist, die zur festen Montage der Dialysezelle an einer gegenüber der In-vitro-Freisetzungstestapparatur ortsfesten Montageposition ausgelegt ist.

    摘要翻译： 是在用于确定的释放行为，如组合物的物质，例如药物，其具有的细胞内分子的载体，如脂质体，胶束或纳米颗粒，附针对接收物质流体的物质和释放速率的体外释放试验装置的渗析池 一个小区内部至少部分地限制透析膜，其保留结合的物质，是可渗透的释放到接收物质流体物质和接收物质流体是至少部分透明的，它提供的是，透析膜被附接到支撑结构的固定安装的渗析池的 一个被设计相对于所述的体外释放试验装置静止安装位置。