公开(公告)号：WO2008082369A3

公开(公告)日：2008-07-10

申请号：PCT/SI2007/000040

申请日：2007-11-22

Applicant: KEMIJSKI INSTITUT ,  PROSEK, Mirko ,  SMIDOVNIK, Andrej ,  FIR, Maja ,  GOLC-WONDRA, Alenka ,  ZMITEK, Janko ,  KOSTANJEVEC, Bostjan ,  DONSA, Bostjan ,  VINDIS ZELENKO, Brigita

Inventor： PROSEK, Mirko ,  SMIDOVNIK, Andrej ,  FIR, Maja ,  GOLC-WONDRA, Alenka ,  ZMITEK, Janko ,  KOSTANJEVEC, Bostjan ,  DONSA, Bostjan ,  VINDIS ZELENKO, Brigita

IPC: A23K1/16 ,  A61K31/122

Abstract: This invention relates to a novel use of coenzyme Q10 in animal husbandry, as additive or supplement to animal feed/fodder and animal beverages, and to the enrichment of animal tissues, especially meat with coenzyme Q10. A further object of this invention is animal tissue enriched with coenzyme Q10. Animal tissues within the scope of this invention are in addition to all types of meat, bones, skins and the like, poultry eggs and the milk of dairy animals as well. The invention comprises a use of coenzyme Q10 to improve the effectiveness of animal husbandry, and the production of animal tissues with an increased content of said enzyme, wherein the intake of coenzyme Q10 by the animals is performed by consumption or direct administration into or onto the body, wherein coenzyme Q10 is in a convenient water-soluble or water-insoluble form, preferably in the form of enhanced bioavailability, and the animals consume the coenzyme Q10 or receive it in another manner during all their lifetime or at a determined period any time during their lifetime, preferably during their growth phase and/or at an optional period prior to slaughter, more preferably during growth phases and/or 3-90 days prior to slaughter, and that the animals consume 1-1000 mg of coenzyme Q10 per kg of body weight daily, preferably 1-50 mg, more preferably 1-20 mg. Coenzyme Q10 is added to animal feed/fodder or beverage. The feed/fodder is any nutrient for animals, unprocessed or processed, natural or artificial and industrially manufactured, respectively.

公开(公告)号：WO2007030083A3

公开(公告)日：2007-03-15

申请号：PCT/SI2006/000028

申请日：2006-08-23

Applicant: KEMIJSKI INSTITUT ,  JERALA, Roman ,  MANCEK KEBER, Mateja ,  BREMSAK, Robert

Inventor： JERALA, Roman ,  MANCEK KEBER, Mateja ,  BREMSAK, Robert

IPC: A61P31/00 ,  A61K31/185 ,  A61K31/352 ,  A61K31/4015

Abstract: The invention presents the applications of compounds, which contain a combination of a hydrophobic group with a group, which is reactive with the cysteine residue for the binding to protein MD-2. The compounds are covalently linked to MD-2, which is either free or in the complex with other molecules. The said compounds replace or prevent binding of other ligands, especially bacterial endotoxin (lipopolysaccharide-LPS), which can otherwise lead towards unwanted activation of the immune response and acute or chronic inflammatory diseases.

公开(公告)号：WO2005023854A2

公开(公告)日：2005-03-17

申请号：PCT/SI2004/000028

申请日：2004-08-25

Applicant: KEMIJSKI INSTITUT ,  LEGISA, Matic ,  BENCINA, Mojca

Inventor： LEGISA, Matic ,  BENCINA, Mojca

IPC: C07K14/38

CPC classification number: C12N9/1205

Abstract: The invention deals with the shorter fragment of 6-phosphofructo-l-kinase (PFK1) with molecular mass between 49 and 55 kDa that is not significantly inhibited by citric acid and/or its salts and is post-translationally modified. The 49-52 kDa fragment that is formed by post-translational modifications of the fungal enzyme retains positive regulatory properties and is activated by specific activators, yet is no more sensitive to inhibition by citric acid a substance that in higher organisms acts as a feed back inhibitor. The invention describes a procedure that includes the introduction of the gene coding for a shorter fragment into the eukaryotic cells with an aim to increase the metabolic flow through the glycolysis and concomitantly to increase the rate of primary and secondary metabolites synthesis. The invention describes the use of the shorter fragment in biotechnological procedures for production of primary and secondary metabolites.

Abstract translation: 本发明涉及分子质量在49和55kDa之间的6-磷酸果糖-1-激酶（PFK1）的较短片段，其不被柠檬酸和/或其盐显着抑制并被翻译后修饰。 通过真菌酶的翻译后修饰形成的49-52kDa片段保留阳性调节性质，并被特定的活化剂活化，但​​对柠檬酸的抑制没有更敏感，在高等生物体中作为反馈物质 抑制剂。 本发明描述了一种方法，其包括将编码较短片段的基因引入真核细胞中，目的是增加通过糖酵解的代谢流，并且伴随地增加初级和次级代谢物合成的速率。 本发明描述了生物技术方法中较短片段用于生产初级和次级代谢物的用途。

公开(公告)号：WO2021032759A1

公开(公告)日：2021-02-25

申请号：PCT/EP2020/073143

申请日：2020-08-19

Applicant: KEMIJSKI INSTITUT

Inventor： JERALA, Roman ,  LAINŠCEK, Duško

IPC: C12N15/10 ,  C12N15/62 ,  C12N9/22 ,  C12N9/78 ,  C12N15/85 ,  C12N5/10 ,  A61K31/70 ,  A61K48/00

Abstract: The invention provides a method for enhanced genome engineering using CRISPR/Cas system tethered to exonucleases via different systems. By connecting Cas9 and exonucleases via heterodimeric peptides that form coiled-coils or via heterodimerization systems, greater indel mutations at desired genomic region occurs, resulting in higher genome editing rate. This novel improved CRISPR/Cas system can be exploited in different cell origins and organisms in various fields, where genome engineering is required and desired.

公开(公告)号：WO2020007980A1

公开(公告)日：2020-01-09

申请号：PCT/EP2019/067994

申请日：2019-07-04

Applicant: KEMIJSKI INSTITUT

Inventor： PAVLIN, Nejc ,  DOMINKO, Robert

IPC: H01M4/04 ,  H01M4/62

Abstract: The present invention provides an anode interfacial protective layer, made from silylated cellulose optionally including ion conductive additive(s) and methods for their fabrication. The protective layer is capable of protecting common anode materials like lithium and lithium alloys from destructive reactions with other battery components, and in that way, enhance performance of a battery cell in which the protective layer is incorporated.

公开(公告)号：WO2017208268A1

公开(公告)日：2017-12-07

申请号：PCT/IT2016/000140

申请日：2016-05-30

Applicant: INSER ENERGIA S.P.A. ,  KEMIJSKI INSTITUT LJUBLJANA

Inventor： FAUSSONE, Gian Claudio ,  GRILC, Miha ,  LIKOZAR, Blaz

IPC: C10G31/09 ,  C10G1/00 ,  C10G1/02 ,  C10G1/08

Abstract: A process is described for removing ashes from biomasses, through a system (1) comprising mixing means (10) and (20), filtering means (30) and distilling means (40) and at least one storage system (50) to allow separating and removing ashes from biomasses, comprising the following steps: depolymerizing organic material present in the biomass and separating the organic material, depolymerized and dissolved in the solvent, with respect to a solid ash-rich residue; filtering the organic material, depolymerized and dissolved in the solvent; distilling and regenerating the solvent present in an environment with high temperature; and extracting and storing biomass without ashes. A system (1) for removing ashes from biomasses is further described.

Abstract translation: 描述了用于通过包括混合装置（10）和（20），过滤装置（30）和蒸馏装置（40）以及至少一个混合装置（10）的系统（1）从生物质中去除灰烬的过程。 包括以下步骤：使生物质中存在的有机物质解聚，并相对于固体的富含灰分的残余物分离解聚并溶解在溶剂中的有机物质; 过滤有机材料，解聚并溶解在溶剂中; 蒸馏和再生存在于高温环境中的溶剂; 并提取并储存生物质而不会产生灰烬。 进一步描述了用于从生物质中去除灰烬的系统（1）。

公开(公告)号：WO2015150503A1

公开(公告)日：2015-10-08

申请号：PCT/EP2015/057274

申请日：2015-04-01

Applicant: KEMIJSKI INSTITUT

Inventor： CRNIVEC OSOJNIK, Ilja, Gasan, ,  DJINOVIC, Petar ,  PINTAR, Albin ,  ERJAVEC, Bostjan

IPC: B01J37/03 ,  B01J37/18 ,  B01J21/06 ,  B01J23/75 ,  B01J23/755 ,  B01J35/02 ,  B01J35/10 ,  B01J37/02 ,  C01B3/40

CPC classification number: B01J21/066 ,  B01J23/75 ,  B01J23/755 ,  B01J35/02 ,  B01J35/1014 ,  B01J35/1019 ,  B01J35/1023 ,  B01J35/1028 ,  B01J35/1038 ,  B01J35/1042 ,  B01J35/1047 ,  B01J37/0203 ,  B01J37/03 ,  B01J37/18 ,  B01J2523/00 ,  C01B3/40 ,  Y02P20/52

Abstract: The invention applies to the field of generating energy products and chemicals with high added value from renewable and alternative sources and examines the conversion of gas mixtures of methane and carbon dioxide into syngas. In particular there are provided a carrier material for carrying active metal phases, a solid catalyst, and processes for forming them. A carrier material of the invention comprises a homogenous solid solution of Ce x Zr 1 - x O 2-y having a face-centred cubic unit cell crystalline structure; wherein x is greater than 0 and less than 1 and y is from 0 to 1; and wherein x is not 0.5.

Abstract translation: 本发明适用于从可再生和替代来源产生具有高附加值的能源产品和化学品的领域，并考察了甲烷和二氧化碳的气体混合物转化为合成气。 特别地，提供了用于承载活性金属相的载体材料，固体催化剂及其形成方法。 本发明的载体材料包含具有面心立方晶胞结晶结构的CexZr1-xO2-y均匀固溶体; 其中x大于0且小于1且y为0至1; 并且其中x不为0.5。

公开(公告)号：WO2015137888A1

公开(公告)日：2015-09-17

申请号：PCT/SI2014/000053

申请日：2014-09-25

Applicant: KEMIJSKI INSTITUT

Inventor： KUNAVER, Matjaž ,  KOS, Tomaž ,  ANŽLOVAR, Alojz ,  ŽAGAR, Ema ,  HUSKIĆ, Miroslav

IPC: C08H8/00 ,  C08L1/04

CPC classification number: C08L1/04 ,  C08H8/00

Abstract: Method for the production of nanocrystalline cellulose from a cellulose-containing starting material, said method comprising contacting said starting material with a first solvent mixture comprising at least one first organic solvent and an acid, catalyst, for a period of time sufficient to dissolve components of said starting material other than nanocrystalline cellulose in said solvent mixture, thereby obtaining nanocrystalline cellulose dispersed in a dissolution mixture; adding a second solvent mixture to said nanocrystalline cellulose dispersed in a dissolution mixture, said second solvent mixture comprising at least one second organic solvent and less than 10% (wt.) water, thereby obtaining nanocrystalline cellulose in a diluted dissolution mixture; separating said nanocrystalline cellulose from said diluted dissolution mixture by at least one filtration or centrifugation step.

Abstract translation: 用于从含纤维素原料生产纳米晶纤维素的方法，所述方法包括使所述起始材料与包含至少一种第一有机溶剂和酸的催化剂的第一溶剂混合物接触一段足以溶解 在所述溶剂混合物中除了纳米晶纤维素之外的原料，由此获得分散在溶解混合物中的纳米晶纤维素; 向分散在溶解混合物中的所述纳米结晶纤维素添加第二溶剂混合物，所述第二溶剂混合物包含至少一种第二有机溶剂和小于10％（重量）的水，从而在稀释的溶解混合物中获得纳米晶体纤维素; 通过至少一个过滤或离心步骤将所述纳米晶纤维素与所述稀释的溶解混合物分离。

公开(公告)号：WO2015009248A2

公开(公告)日：2015-01-22

申请号：PCT/SI2014/000042

申请日：2014-07-10

Applicant: KEMIJSKI INSTITUT ,  SILKEM D.O.O.

Inventor： FAKIN, Tomaž ,  RISTIĆ, Alenka ,  KAUČIČ, Venčeslav ,  ZABUKOVEC LOGAR, Nataša ,  GOZNIK, Ivan ,  HORVAT, Andrej

CPC classification number: B01J29/40 ,  B01J2229/62 ,  C01B39/38

Abstract: The invention of the preparation of zeolite ZSM-5 granulates free from inorganic binder provides a synthetic process for the preparation of highly crystalline zeolite granulates without using inorganic binder from amorphous sodium aluminosilicate granules. This invention falls within the scope of the inorganic chemical technology. Granules were prepared from the powdered dried amorphous sodium aluminosilicate gel without containing organic structure directing agent. The proposed synthesis process provides the preparation of the amorphous sodium aluminosilicate gel from sodium water-glass, sodium aluminate using less than 5% of zeolite ZSM-5 seed crystals. The pH value of the gel is adjusted using dilute sulfuric (VI) acid. Dried amorphous sodium aluminosilicate gel is granulated using sodium water-glass. Thermally treated amorphous sodium aluminosilicate granulate is transformed in the alkaline solution into crystalline zeolite ZSM-5 granulate by the hydrothermal synthesis from 22 to 28 hours at a temperature from 170 to 190°C.

Abstract translation: 制备不含无机粘合剂的沸石ZSM-5颗粒的发明提供了一种合成方法，用于制备高度结晶的沸石颗粒而不使用无定形硅酸铝钠颗粒的无机粘合剂。 本发明属于无机化学技术的范围。 由不含有机结构导向剂的粉状干燥无定形硅铝酸钠凝胶制备颗粒。 所提出的合成方法使用少于5％的沸石ZSM-5晶种，由钠水玻璃铝酸钠制备无定形硅铝酸钠凝胶。 使用稀硫酸（VI）酸调节凝胶的pH值。 使用钠水玻璃将干燥的无定形硅铝酸钠凝胶粒化。 热处理的无定形硅铝酸钠颗粒在碱性溶液中通过水热合成在170至190℃的温度下22至28小时转化成结晶沸石ZSM-5颗粒。

公开(公告)号：WO2007123498A2

公开(公告)日：2007-11-01

申请号：PCT/SI2007/000007

申请日：2007-02-27

Applicant: KEMIJSKI INSTITUT ,  LEGISA, Matic ,  BENCINA, Mojca ,  TEVZ, Gregor ,  CAPUDER, Maja ,  MLAKAR, Tina ,  OVEN, Darija

Inventor： LEGISA, Matic ,  BENCINA, Mojca ,  TEVZ, Gregor ,  CAPUDER, Maja ,  MLAKAR, Tina ,  OVEN, Darija

IPC: C07K14/43

CPC classification number: C12Y207/01011 ,  C12N9/1205

Abstract: The invention deals with mutated truncated mX- pfk A gene encoding shorter fragment of 6- phosphofructo-1 -kinase (PFKl), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49 -52 kDa fragment encoded by mt- pflt k gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.

Abstract translation: 本发明涉及编码6-磷酸果糖-1-激酶（PFK1）的较短片段的突变的截短的mX-pfkA基因，其不需要磷酸化蛋白质分子 对于活化而言，其不被柠檬酸和/或其盐和ATP分子显着抑制。 由mtflflk基因编码的活性49-52kDa片段保留天然蛋白质的正调控性质，并且在特异性激活剂存在下被激活，而柠檬酸和ATP是重要的代谢物，其作为 高等生物中的反馈抑制剂不会降低其活性。 本发明涉及在生物技术方法中使用修饰的较短片段来制备一级和二级代谢物。