公开(公告)号：WO2012160191A2

公开(公告)日：2012-11-29

申请号：PCT/EP2012/059826

申请日：2012-05-25

Applicant: DSM IP ASSETS B.V. ,  DUVAL, Stephane ,  IMMIG, Irmgard ,  KINDERMANN, Maik ,  WEBER, Gilbert

Inventor： DUVAL, Stephane ,  IMMIG, Irmgard ,  KINDERMANN, Maik ,  WEBER, Gilbert

IPC: A23K1/16 ,  A23K1/17 ,  A23K1/18

CPC classification number: A61K31/21 ,  A23K20/10 ,  A23K20/105 ,  A23K20/195 ,  A23K50/10 ,  A61K9/0053 ,  A61K31/04 ,  A61K31/215 ,  A61K31/22 ,  A61K31/221 ,  A61K31/23 ,  A61K31/235 ,  A61K31/24 ,  A61K31/255 ,  A61K31/275 ,  A61K31/34 ,  A61K31/35 ,  A61K31/351 ,  A61K31/44 ,  A61K31/4406 ,  A61K45/06 ,  Y02P60/56

Abstract: The present invention relates to the field of reduction of methane emission in ruminants. Particularly, it relates to the use of a feed composition or a feed additive comprising at least one antibiotic and at least one organic molecule substituted at any position with at least one nitrooxy group for reducing the production of methane emanating from the digestive activities of ruminants, and/or to improve the ruminant performance.

Abstract translation: 本发明涉及反刍动物甲烷排放减少的领域。 特别地，本发明涉及使用包含至少一种抗生素和至少一种在任何位置与至少一个硝基氧基取代的有机分子的饲料组合物或饲料添加剂，用于减少由反刍动物的消化活性产生的甲烷的产生， 和/或提高反刍动物的表现。

公开(公告)号：WO2011045418A1

公开(公告)日：2011-04-21

申请号：PCT/EP2010/065533

申请日：2010-10-15

Applicant: DSM IP ASSETS B.V. ,  DUVAL, Stephane ,  KINDERMANN, Maik

Inventor： DUVAL, Stephane ,  KINDERMANN, Maik

IPC: A23K1/18 ,  A23K1/16

CPC classification number: A23K50/10 ,  A23K20/10 ,  A23K20/105 ,  Y02P60/56

Abstract: The present invention relates to a method for reducing the production of methane emanating from the digestive activities of a ruminant by using as active compound at least one azido alkanoic acid and/or derivative thereof, which is administrated to the animal together with the feed. The invention also relates to the use of these ingredients in feed and feed additives such as premix, concentrates and total mixedration (TMR) or in the form of a bolus.

Abstract translation: 本发明涉及通过使用与饲料一起施用于动物的至少一种叠氮基链烷酸和/或其衍生物作为活性化合物来减少由反刍动物的消化活性产生的甲烷的产生方法。 本发明还涉及这些成分在饲料和饲料添加剂中的用途，例如预混物，浓缩物和总混合（TMR）或以推注的形式。

公开(公告)号：WO2012160191A3

公开(公告)日：2013-01-31

申请号：PCT/EP2012059826

申请日：2012-05-25

Applicant: DSM IP ASSETS BV ,  DUVAL STEPHANE ,  IMMIG IRMGARD ,  KINDERMANN MAIK ,  WEBER GILBERT

Inventor： DUVAL STEPHANE ,  IMMIG IRMGARD ,  KINDERMANN MAIK ,  WEBER GILBERT

IPC: A23K1/16 ,  A23K1/17 ,  A23K1/18

CPC classification number: A61K31/21 ,  A23K20/10 ,  A23K20/105 ,  A23K20/195 ,  A23K50/10 ,  A61K9/0053 ,  A61K31/04 ,  A61K31/215 ,  A61K31/22 ,  A61K31/221 ,  A61K31/23 ,  A61K31/235 ,  A61K31/24 ,  A61K31/255 ,  A61K31/275 ,  A61K31/34 ,  A61K31/35 ,  A61K31/351 ,  A61K31/44 ,  A61K31/4406 ,  A61K45/06 ,  Y02P60/56

Abstract: The present invention relates to the field of reduction of methane emission in ruminants. Particularly, it relates to the use of a feed composition or a feed additive comprising at least one antibiotic and at least one organic molecule substituted at any position with at least one nitrooxy group for reducing the production of methane emanating from the digestive activities of ruminants, and/or to improve the ruminant performance.

Abstract translation: 本发明涉及反刍动物甲烷排放减少的领域。 特别地，本发明涉及使用包含至少一种抗生素和至少一种在任何位置与至少一个硝基氧基取代的有机分子的饲料组合物或饲料添加剂，用于减少由反刍动物的消化活性产生的甲烷的产生， 和/或提高反刍动物的表现。

公开(公告)号：WO2008012296A1

公开(公告)日：2008-01-31

申请号：PCT/EP2007/057597

申请日：2007-07-24

Applicant: EPFL - ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE ,  GAUTIER, Arnaud ,  JOHNSSON, Kai ,  KINDERMANN, Maik ,  JUILLERAT, Alexandre ,  BEAUFILS, Florent

Inventor： GAUTIER, Arnaud ,  JOHNSSON, Kai ,  KINDERMANN, Maik ,  JUILLERAT, Alexandre ,  BEAUFILS, Florent

IPC: C07D239/46 ,  C07K14/435 ,  C12Q1/48

CPC classification number: G01N33/581 ,  C07D239/47 ,  C07D403/14 ,  C07D405/12 ,  C07D487/04 ,  C12N9/12 ,  C12Q1/48

Abstract: The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O 6 -alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substrates according of the invention are substituted cytosines of formula (I) wherein R 1 is an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to OCH 2 -; R 2 is a linker; and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring label L from these substrates of formula (I) to ACTs and ACT fusion proteins. The system of ACT-compound of formula (I) is particularly suitable for double labelling studies together with the known system O 6 -alkylguanine-DNA alkyltransferase (AGT)-benzylguanines.

Abstract translation: 本发明涉及新的蛋白质，称为烷基胞嘧啶转移酶（ACTs），衍生自O 6 - 烷基鸟嘌呤-DNA烷基转移酶，以及用于特异性转移标记于这些ACT的ACT的底物和包含它们的融合蛋白。 根据本发明的底物是式（I）的取代的胞嘧啶，其中R 1是芳族或杂芳族基团，或任选取代的不饱和烷基，环烷基或杂环基，其双键连接到OCH _{2 - ; R 2是连接基; L是标签或多个相同或不同的标签。 本发明还涉及将标记L从这些式（I）底物转移到ACT和ACT融合蛋白的方法。 式（I）的ACT-化合物的系统特别适用于已知的系统O 6 - 烷基鸟嘌呤-DNA烷基转移酶（AGT） - 苄基胍的双重标记研究。}

公开(公告)号：WO2011070133A1

公开(公告)日：2011-06-16

申请号：PCT/EP2010/069338

申请日：2010-12-10

Applicant: DSM IP ASSETS B.V. ,  DUVAL, Stephane ,  KINDERMANN, Maik

Inventor： DUVAL, Stephane ,  KINDERMANN, Maik

IPC: A23K1/00 ,  A23K1/16 ,  A23K1/18

CPC classification number: C07C203/04 ,  A23K20/105 ,  A23K20/174 ,  A23K50/10 ,  A61K31/21 ,  A61K31/215 ,  A61K45/06 ,  C07C235/06 ,  Y02P60/56

Abstract: The present invention relates to a method for reducing the production of methane emanating from the digestive activities of a ruminant and or for improving ruminant animal performance by using, as active compound at least one nitrooxy alkanoic acid and/or derivative thereof, which is administrated to the animal together with the feed. The invention also relates to the use of these compounds in feed and feed additives such as premix, concentrates and total mixed ration (TMR) or in the form of a bolus.

Abstract translation: 本发明涉及一种通过使用至少一种硝基氧基链烷酸和/或其衍生物作为活性化合物来降低由反刍动物的消化活性产生的甲烷的产生和/或用于改善反刍动物性能的方法， 动物与饲料一起。 本发明还涉及这些化合物在饲料和饲料添加剂如预混物，浓缩物和总混合比例（TMR）中或在丸剂形式中的用途。

公开(公告)号：WO2006114409A9

公开(公告)日：2007-10-18

申请号：PCT/EP2006061798

申请日：2006-04-25

Applicant: COVALYS BIOSCIENCES AG ,  KINDERMANN MAIK ,  SCHWAB MARKUS

Inventor： KINDERMANN MAIK ,  SCHWAB MARKUS

IPC: C07D239/46

CPC classification number: C07D239/47 ,  C07D405/12 ,  C07D495/04

Abstract: The invention relates to pyrimidines suitable as substrates for O 6 -alkylguanine-DNA alkyltransferases (AGT) of formula (I) wherein R 1 is hydrogen, lower alkyl, halogen, cyano, trifluoromethyl or azido; R 2 is a linker; and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring a label from pyrimidines of formula (I) to O 6 -alkylguanine-DNA alkyltransferases (AGT) and AGT fusion proteins.

Abstract translation: 本发明涉及适合作为其中R 1为氢的低级烷基鸟嘌呤-DNA烷基转移酶（AGT）的底物的嘧啶，其中R 1为氢，低级烷基，卤素，氰基 ，三氟甲基或叠氮基; R 2是连接体; L是标签或多个相同或不同的标签。 本发明还涉及将标记从式（I）的嘧啶转移到O 6 - 烷基鸟嘌呤-DNA烷基转移酶（AGT）和AGT融合蛋白的方法。

公开(公告)号：WO2006114409A1

公开(公告)日：2006-11-02

申请号：PCT/EP2006/061798

申请日：2006-04-25

Applicant: COVALYS BIOSCIENCES AG ,  KINDERMANN, Maik ,  SCHWAB, Markus

Inventor： KINDERMANN, Maik ,  SCHWAB, Markus

IPC: C07D239/46

CPC classification number: C07D239/47 ,  C07D405/12 ,  C07D495/04

Abstract: The invention relates to pyrimidines suitable as substrates for O 6 -alkylguanine-DNA alkyltransferases (AGT) of formula (I) wherein R 1 is hydrogen, lower alkyl, halogen, cyano, trifluoromethyl or azido; R 2 is a linker; and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring a label from pyrimidines of formula (I) to O 6 -alkylguanine-DNA alkyltransferases (AGT) and AGT fusion proteins.

Abstract translation: 本发明涉及适合作为其中R 1为氢的低级烷基鸟嘌呤-DNA烷基转移酶（AGT）的底物的嘧啶，其中R 1为氢，低级烷基，卤素，氰基 ，三氟甲基或叠氮基; R 2是连接体; L是标签或多个相同或不同的标签。 本发明还涉及将标记从式（I）的嘧啶转移到O 6 - 烷基鸟嘌呤-DNA烷基转移酶（AGT）和AGT融合蛋白的方法。

公开(公告)号：WO2004031405A1

公开(公告)日：2004-04-15

申请号：PCT/EP2003/010889

申请日：2003-10-01

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL) ,  KINDERMANN, Maik ,  JOHNSSON, Kai ,  BIERI, Christoph

Inventor： KINDERMANN, Maik ,  JOHNSSON, Kai ,  BIERI, Christoph

IPC: C12Q1/48

CPC classification number: C07D209/48 ,  C07D333/28 ,  C07D473/16 ,  C07D473/18 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/48

Abstract: The present invention relates to methods of transferring a label from novel substrates to O 6 -alkylguanine-DNA alkyltransferases (AGT) and O 6 -alkylguanine-DNA alkyltransferase fusion proteins, and to novel substrates suitable in such methods. Proteins of interest are incorporated into an AGT fusion protein, the AGT fusion protein is contacted with particular AGT substrates carrying a label, and the AGT fusion protein is detected and optionally further manipulated using the label in a system designed for recognising and/or handling the label. The particular AGT substrates used in the method of the invention are O 6 -substituted guanine derivatives or related nitrogen containing hydroxy-heterocycles and their sulfur analogs wherein the O 6 -substituent is an activated methyl derivative suitable for transfer from guanine or the corresponding heterocycle to AGT, and further carrying a label or a plurality of same or different labels. The invention relates also to the novel AGT substrates as such, to methods of manufacture of such novel substrates, and to intermediates useful in the synthesis of such novel AGT substrates.

Abstract translation: 本发明涉及将标记从新型底物转移至O 6 - 烷基鸟嘌呤-DNA烷基转移酶（AGT）和O 6 - 烷基鸟嘌呤-DNA烷基转移酶融合蛋白的方法，以及适用于这些方法的新型底物。 将感兴趣的蛋白质并入AGT融合蛋白中，AGT融合蛋白与携带标记的特定AGT底物接触，并且可以使用设计用于识别和/或处理AGT融合蛋白的系统中的标记来检测和任选地进一步操纵AGT融合蛋白。 标签。 在本发明方法中使用的特定AGT底物是O 6取代的鸟嘌呤衍生物或相关的含氮羟基 - 杂环及其硫类似物，其中O 6 - 取代基是适合于从鸟嘌呤或鸟嘌呤转移的活化的甲基衍生物 对应于AGT的杂环，并进一步携带标签或多个相同或不同的标记。 本发明还涉及新颖的AGT底物，关于制备这种新型底物的方法以及可用于合成这种新型AGT底物的中间体。

公开(公告)号：WO2009103432A3

公开(公告)日：2010-01-07

申请号：PCT/EP2009000815

申请日：2009-02-06

Applicant: SANOFI AVENTIS ,  WENDT KARL-ULRICH ,  KINDERMANN MAIK ,  MINIEJEW CATHERINE ,  GLOBISCH ANJA

Inventor： WENDT KARL-ULRICH ,  KINDERMANN MAIK ,  MINIEJEW CATHERINE ,  GLOBISCH ANJA

IPC: C07D277/42 ,  A61K31/495 ,  C07D403/12 ,  C07D413/12

CPC classification number: C07D295/215 ,  C07D209/14 ,  C07D311/82 ,  C07D487/04 ,  C07D495/04

Abstract: The present invention relates to molecular probes of the formula (I) L1-R1-L-A-X as defined herein that allow for the observation of the catalytic activity of a selected capase, cathepsin, MMP and carboxypeptidase in in vitro assays, in cells or in multicellular organisms, a method for their preparation and the use thereof.

Abstract translation: 本发明涉及如本文所定义的式（I）L1-R1-LAX的分子探针，其允许在体外测定，细胞或多细胞中观察所选择的酶，组织蛋白酶，MMP和羧肽酶的催化活性 生物体，其制备方法及其用途。

公开(公告)号：WO2005085470A9

公开(公告)日：2006-10-05

申请号：PCT/EP2005050900

申请日：2005-03-01

Applicant: EPFL ECOLE POLYTECHNIQUE FEDERALE LAUSANNE ,  JACCARD HUGHES ,  JOHNSSON KAI ,  KINDERMANN MAIK ,  SIELAFF INDIA CHRISTINA

Inventor： JACCARD HUGHES ,  JOHNSSON KAI ,  KINDERMANN MAIK ,  SIELAFF INDIA CHRISTINA

IPC: C07D473/18 ,  C12Q1/48

CPC classification number: C07D473/18

Abstract: The invention relates to substrates for O 6 -alkylguanine-DNA alkyltransferases (AGT) of formula R 1 -A-X-CH 2 -R 3 -R 4 -L 1 , wherein A is a group recognized by AGT as a substrate, X is oxygen or sulfur, R 1 is a group -R 2 -L 2 or a group R 5 , R 2 and R 4 are, independently of each other, a linker, R 3 is an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to CH 2 , R 5 is arylmethyl or heteroarylmethyl or an optionally substituted cycloalkyl, cycloalkenyl or heterocyclyl group, L 1 is a label, a plurality of same or different labels, a bond connecting R 4 to A forming a cyclic substrate , or a further group -R 3 CH 2 -X-A-R 1, and L 2 is a label or a plurality of same or different labels. The invention further relates to methods of transferring a label from these substrates to O 6-

Abstract translation: 本发明涉及式R 1 -AX-CH 2 -R Sub的O 6 - 烷基鸟嘌呤-DNA烷基转移酶（AGT）的底物 其中A是被AGT识别为底物的基团，X是氧或硫，R 1 是基团-R 2→L 2或基团R 5，R 2和/或 R 4彼此独立地是连接基，R 3是芳族或杂芳族基团，或任选取代的不饱和烷基，环烷基或杂环基与双 与CH 2 R 2连接的键是R 5是芳基甲基或杂芳基甲基或任选取代的环烷基，环烯基或杂环基，L 1是标记， 多个相同或不同的标记，连接R 4与形成环状基底的A的键，或另外的基团-R 3 3 CH 2 -XAR 1，＆lt; / 2＆gt;和L 2 2是标签或多个相同或不同的标签 。 本发明还涉及将标记从这些底物转移到O6-6的方法