公开(公告)号：WO2012155097A3

公开(公告)日：2013-01-03

申请号：PCT/US2012037626

申请日：2012-05-11

Applicant: LUMINEX CORP ,  KOZMA LYNN ,  FAIRCHILD MAUREEN ,  CHING JESUS

Inventor： KOZMA LYNN ,  FAIRCHILD MAUREEN ,  CHING JESUS

IPC: G01N33/53

CPC classification number: B01L7/52 ,  B01L3/0217 ,  B01L3/527 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/142 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2400/043 ,  B01L2400/0469 ,  B01L2400/0487 ,  B01L2400/0672 ,  G01N21/13 ,  G01N21/6428 ,  G01N35/00 ,  G01N2021/0328

Abstract: Systems and methods for producing an evaporation barrier in a polymerase chain reaction (PCR) vial. Beads with a particular distribution in diameters can be used to produce a barrier for reducing the evaporation of liquid PCR samples within the PCR vial. The beads can be pre-filled in the PCR vial. Liquid samples and/or liquid reagents can be introduced in the PCR vial pre-filled with the beads, such that the beads can be driven to the surface of the liquid PCR sample through the buoyancy of the beads.

Abstract translation: 用于在聚合酶链反应（PCR）小瓶中产生蒸发屏障的系统和方法。 可以使用具有特定直径分布的珠来产生用于减少PCR小瓶内液体PCR样品的蒸发的屏障。 珠可以预先填充在PCR小瓶中。 可以将液体样品和/或液体试剂引入预填充珠粒的PCR小瓶中，使得珠可以通过珠的浮力被驱动到液体PCR样品的表面。

公开(公告)号：WO2007149903A2

公开(公告)日：2007-12-27

申请号：PCT/US2007/071641

申请日：2007-06-20

Applicant: CEPHEID ,  KOZMA, Lynn ,  SWENSON, David

Inventor： KOZMA, Lynn ,  SWENSON, David

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/107 ,  C12Q2561/113 ,  C12Q2525/186 ,  C12Q2537/149 ,  C12Q2527/101

Abstract: The present invention provides methods and kits for conducting multiplex nucleic acid amplification reactions by controlling target sequence replication times. In one aspect, such control is exerted by selecting different lengths of target polynucleotides for amplification. In another aspect, control is exerted by providing sequence-specific polymerase inhibitors, such as specific blocking oligonucleotides. In accordance with the invention, multiple target polynucleotides can be sequentially amplified in an amplification reaction conducted in different stages, wherein amplification of sequences with longer replication times is permitted in one stage but precluded in other stages by modifying polymerase extension times in the course of the reaction.

Abstract translation: 本发明提供了通过控制靶序列复制时间进行多重核酸扩增反应的方法和试剂盒。 在一个方面，通过选择不同长度的靶多核苷酸进行扩增来发挥这种控制。 另一方面，通过提供序列特异性聚合酶抑制剂，例如特异性阻断寡核苷酸来进行对照。 根据本发明，可以在不同阶段进行的扩增反应中顺序扩增多个靶多核苷酸，其中一个阶段允许扩增具有较长复制时间的序列，但是在其他阶段通过在 反应。

公开(公告)号：WO2012155097A2

公开(公告)日：2012-11-15

申请号：PCT/US2012/037626

申请日：2012-05-11

Applicant: LUMINEX CORPORATION ,  KOZMA, Lynn ,  FAIRCHILD, Maureen ,  CHING, Jesus

Inventor： KOZMA, Lynn ,  FAIRCHILD, Maureen ,  CHING, Jesus

IPC: C12Q1/68 ,  C12M1/00 ,  C12P19/34

CPC classification number: B01L7/52 ,  B01L3/0217 ,  B01L3/527 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/142 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2400/043 ,  B01L2400/0469 ,  B01L2400/0487 ,  B01L2400/0672 ,  G01N21/13 ,  G01N21/6428 ,  G01N35/00 ,  G01N2021/0328

Abstract: The embodiments described herein relate to systems and methods for producing an evaporation barrier in a PCR vial. In some embodiments, beads with a particular distribution in diameters can be used to produce a barrier for reducing the evaporation of liquid PCR samples within the PCR vial. In some embodiments, the beads can be pre-filled in the PCR vial. In use, liquid samples and/or liquid reagents can be introduced in the PCR vial prefilled with the beads, such that the beads can be driven to the surface of the liquid PCR sample through the buoyancy of the beads.

Abstract translation: 本文描述的实施方案涉及在PCR小瓶中产生蒸发屏障的系统和方法。 在一些实施方案中，具有特定分布直径的珠粒可用于产生用于减少PCR小瓶内的液体PCR样品的蒸发的屏障。 在一些实施方案中，珠可以预先填充在PCR小瓶中。 在使用中，可以将液体样品和/或液体试剂引入预填充珠粒的PCR小瓶中，使得可以通过珠的浮力将珠子驱动到液体PCR样品的表面。

公开(公告)号：WO2007149903A3

公开(公告)日：2008-08-07

申请号：PCT/US2007071641

申请日：2007-06-20

Applicant: CEPHEID ,  KOZMA LYNN ,  SWENSON DAVID

Inventor： KOZMA LYNN ,  SWENSON DAVID

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/107 ,  C12Q2561/113 ,  C12Q2525/186 ,  C12Q2537/149 ,  C12Q2527/101

Abstract: The present invention provides methods and kits for conducting multiplex nucleic acid amplification reactions by controlling target sequence replication times. In one aspect, such control is exerted by selecting different lengths of target polynucleotides for amplification. In another aspect, control is exerted by providing sequence-specific polymerase inhibitors, such as specific blocking oligonucleotides. In accordance with the invention, multiple target polynucleotides can be sequentially amplified in an amplification reaction conducted in different stages, wherein amplification of sequences with longer replication times is permitted in one stage but precluded in other stages by modifying polymerase extension times in the course of the reaction.

Abstract translation: 本发明提供了通过控制靶序列复制时间进行多重核酸扩增反应的方法和试剂盒。 在一个方面，通过选择不同长度的靶多核苷酸进行扩增来发挥这种控制。 在另一方面，通过提供序列特异性聚合酶抑制剂，例如特异性阻断寡核苷酸来进行控制。 根据本发明，可以在不同阶段进行的扩增反应中顺序扩增多个靶多核苷酸，其中在一个阶段允许扩增具有较长复制时间的序列，但是在其他阶段通过在 反应。