公开(公告)号：WO2019116033A1

公开(公告)日：2019-06-20

申请号：PCT/GB2018/053609

申请日：2018-12-12

Applicant: OXFORD UNIVERSITY INNOVATION LIMITED

Inventor： WALSH, Edmond ,  COOK, Peter Richard ,  SOITU, Cristian

IPC: B01L3/00

CPC classification number: B01L3/5088 ,  B01L3/502707 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2300/161

Abstract: Methods and apparatus for manufacturing a microfluidic arrangement are disclosed. In one arrangement, a selected region of a substrate surface is modified by moving a probe over the selected region while maintaining contact between the probe and the substrate surface. The modification is such that an equilibrium contact angle of the first liquid is higher than without the modification. The modified selected region forms one or more isolated regions on the substrate surface by surrounding each isolated region. The modified selected region forming a closed path around each isolated region. A microfluidic arrangement is formed that comprises a body of the first liquid in direct contact with each isolated region, and a second liquid in direct contact with, and covering, each body of first liquid and the modified selected region, wherein the second liquid is immiscible with the first liquid, and each body of first liquid is held in shape predominantly by surface tension and substantially fills each isolated region.

公开(公告)号：WO2017001537A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065238

申请日：2016-06-30

Applicant: VENTANA MEDICAL SYSTEMS, INC. ,  F. HOFFMANN-LA ROCHE AG

Inventor： ASHBY, Austin, M. ,  BARNETT, Donald, M. ,  KELLER, Timothy, J. ,  KRAM, Brian, H. ,  MARSHALL, Kevin, D. ,  TSE, Christine ,  WILKINSON, Chad, A.

IPC: G01N1/31 ,  B01F11/00 ,  B01F13/00 ,  G02B21/34

CPC classification number: G01N1/312 ,  B01F11/0071 ,  B01L3/0293 ,  B01L7/00 ,  B01L9/52 ,  B01L2200/025 ,  B01L2200/06 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/0822 ,  B01L2300/1827 ,  B01L2400/0406 ,  G01N35/00029 ,  G01N35/1002 ,  G01N2035/00138 ,  G01N2035/1034 ,  G02B21/34

Abstract: Disclosed are specimen processing systems capable of processing specimens carried on slides. The specimen processing systems comprise opposables having at least one fluid control element (1500, 1501, 1502). The fluid control elements (1500, 1501, 1502) may be positioned between spacers or gapping elements (1450, 1452) and the opposable edges (1454, 1456). The fluid control elements may comprise an edge, such as a beveled edge or a stepped edge, as described herein, and the edge may be continuous or segmented.

Abstract translation: 公开了能够处理载玻片上的标本的样品处理系统。 样本处理系统包括具有至少一个流体控制元件（1500,1501,1502）的对象物。 流体控制元件（1500,1501,1502）可以位于间隔件或间隙元件（1450,1452）和可相对边缘（1454,1456）之间。 流体控制元件可以包括如本文所述的边缘，例如斜边或阶梯边缘，并且边缘可以是连续的或分段的。

公开(公告)号：WO2016201163A1

公开(公告)日：2016-12-15

申请号：PCT/US2016/036787

申请日：2016-06-10

Applicant: TEXAS TECH UNIVERSITY SYSTEM

Inventor： VANAPALLI, Siva, A ,  BITHI, Swastika, S.

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/12 ,  B01L2200/142 ,  B01L2300/041 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2400/049 ,  B01L2400/084

Abstract: A microfluidic device includes a substrate and a cover. The substrate has an inlet port, a first microchannel, one or more parking loops, a second microchannel and an outlet port for each microchannel network. The first microchannel is connected to the inlet port, the second microchannel is connected to the outlet port, the parking loops are connected between the first and second microchannels. Each parking loop includes a parking loop inlet, a parking loop output, a fluidic trap connected between the parking loop inlet and the parking loop outlet, and a bypass microchannel connected to the parking loop inlet and the parking loop outlet. The cover is attached to a top of the substrate and has an inlet opening and an outlet opening through the cover for each microchannel network. The inlet and outlet openings of the cover are disposed above the inlet and outlet ports in the substrate.

Abstract translation: 微流体装置包括基底和盖。 衬底具有用于每个微通道网络的入口，第一微通道，一个或多个停车回路，第二微通道和出口。 第一微通道连接到入口，第二微通道连接到出口，停车回路连接在第一和第二微通道之间。 每个停车回路包括停车回路入口，停车回路输出，连接在停车回路入口和停车回路出口之间的流体回路，以及连接到停车回路入口和停车回路出口的旁路微通道。 盖子连接到基板的顶部，并且具有通过每个微通道网络的盖子的入口开口和出口开口。 盖的入口和出口开口设置在基板中的入口和出口的上方。

公开(公告)号：WO2016097711A1

公开(公告)日：2016-06-23

申请号：PCT/GB2015/053983

申请日：2015-12-14

Applicant: RANDOX LABORATORIES LTD

Inventor： JACKSON, Stuart ,  MCCONNELL, Ivan ,  LAMONT, John ,  FITZGERALD, Peter ,  WHARRY, Marc

IPC: B01L3/00 ,  C12M1/00

CPC classification number: B01L9/52 ,  B01L3/508 ,  B01L2200/0689 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0832 ,  B01L2300/161 ,  B65B7/2871 ,  C12M23/38

Abstract: The present invention is directed to a cap for a biochip storage well. The cap comprises a resilient sealant layer capable, under the application of pressure, of forming a vapour-proof seal with a line contact interface formation extending around the perimeter of a biochip storage well. An assay assembly comprising the cap of the invention and a biochip storage is disclosed. Methods of sealing a biochip storage well using a cap of the invention are also disclosed.

Abstract translation: 本发明涉及一种用于生物芯片存储井的盖。 所述盖包括弹性密封剂层，所述弹性密封剂层能够在施加压力的情况下与围绕生物芯片存储井的周边延伸的线接触界面形成形成防蒸气密封。 公开了一种包括本发明的盖和生物芯片存储器的测定组件。 还公开了使用本发明的盖密封生物芯片储存井的方法。

公开(公告)号：WO2014151996A3

公开(公告)日：2014-12-04

申请号：PCT/US2014026789

申请日：2014-03-13

Applicant: GEN PROBE INC

Inventor： KNIGHT BYRON J ,  BUSE DAVID ,  GROELI JULIAN

IPC: B01L3/00

CPC classification number: G01N35/1002 ,  B01L3/502 ,  B01L3/5082 ,  B01L3/50825 ,  B01L3/5085 ,  B01L3/50853 ,  B01L3/50855 ,  B01L3/523 ,  B01L3/527 ,  B01L2200/02 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/047 ,  B01L2300/048 ,  B01L2300/0609 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/12 ,  B01L2400/0406 ,  B65B69/00 ,  B65D1/0207 ,  B65D1/34 ,  B65D1/36 ,  B65D17/50 ,  B65D39/0017 ,  B65D39/0029 ,  B65D2539/003 ,  C12Q1/686 ,  G01N30/6091 ,  G01N35/0099 ,  G01N35/026 ,  G01N35/10 ,  G01N35/1079 ,  G01N2035/00287 ,  Y10T436/11

Abstract: System, apparatuses, and methods for performing automated reagent-based analysis are provided. Also provided are methods for automated attachment of a cap to a reaction receptacle, and automated removal of a cap from a capped reaction receptacle.

Abstract translation: 提供了用于执行基于自动化试剂的分析的系统，装置和方法。 还提供了用于将盖自动附接到反应容器的方法，以及从加盖的反应容器自动移除盖。

公开(公告)号：WO2014151450A1

公开(公告)日：2014-09-25

申请号：PCT/US2014/025753

申请日：2014-03-13

Applicant: SIEMENS HEALTHCARE DIAGNOSTICS INC.

Inventor： LEDDEN, David, J. ,  CHEN, Dafeng ,  RASMUSSEN, James, E.

IPC: G01N33/50

CPC classification number: B01L3/502753 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/0605 ,  B01L2200/14 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/161 ,  B01L2400/0688 ,  B01L2400/084 ,  G01N1/30

Abstract: A microfluidic distributing device having a plurality of microchannels for the analysis of a fluid sample (such as blood). The microfluidic distributing device has a fluid sample entry port from which subsamples of the fluid sample are distributed to the plurality of microchannels in which fluid subsamples are treated for analysis by test devices.

Abstract translation: 具有用于分析流体样品（例如血液）的多个微通道的微流体分配装置。 微流体分配装置具有流体样品入口端口，流体样品的子样本从该液体样本入口端口分配到多个微通道，在该微通道中流体子样品被处理以供测试装置分析。

公开(公告)号：WO2014108323A1

公开(公告)日：2014-07-17

申请号：PCT/EP2014/000005

申请日：2014-01-06

Applicant: ETH ZURICH

Inventor： DITTRICH, Petra ,  PABST Martin ,  KUESTER Simon

IPC: B01J19/00 ,  G01N33/68 ,  B01L3/02 ,  B01L3/00 ,  G01N35/00 ,  G01N30/02

CPC classification number: B01L3/0262 ,  B01L3/502784 ,  B01L3/5088 ,  B01L2200/0673 ,  B01L2200/142 ,  B01L2400/0487 ,  G01N30/82 ,  G01N35/08 ,  H01J49/0418

Abstract: A method for depositing droplets (24) separated by a first fluid from a channel (20) onto a substrate (10) is disclosed. To prevent evaporation of the droplets and to enable combination of droplets, the substrate is covered with a second fluid, and the outlet end (26) of the channel is immersed in the second fluid. The method can be used as an interface between a separation technique, e.g., liquid chromatography, and an analytical technique such as MALDI-MS. A further chemical dimension can be added by splitting a single peak of the separation technique over a plurality of sample spots and subjecting only selected sample spots to a chemical reaction. Applications include the analysis of post-translational modifications of proteins and peptides.

Abstract translation: 公开了一种用于将通过第一流体从通道（20）分离的液滴（24）沉积到衬底（10）上的方法。 为了防止液滴的蒸发并且能够组合液滴，衬底被第二流体覆盖，并且通道的出口端（26）浸没在第二流体中。 该方法可以用作分离技术（例如液相色谱法）和诸如MALDI-MS的分析技术之间的界面。 可以通过在多个样品点上分离分离技术的单个峰并且仅对选定的样品点进行化学反应来添加另外的化学尺寸。 应用包括对蛋白质和肽的翻译后修饰的分析。

公开(公告)号：WO2013113054A1

公开(公告)日：2013-08-08

申请号：PCT/AU2012/000089

申请日：2012-02-03

Applicant: PYROBETT PTE LTD ,  CORBETT, John ,  CORBETT, SNR, John

Inventor： CORBETT, John ,  CORBETT, SNR, John

IPC: C12Q1/68

CPC classification number: B01L3/502753 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/142 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

Abstract translation: 本发明涉及进行核酸测序的方法和装置。 该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对 约束伙伴 该方法还包括以下步骤：将第一或第二结合配偶体固定或固定到支撑表面，并从所述平台外部的点向试剂分配，其中在分配步骤之后，平台被充分旋转，使得任何残留或 从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。 本发明还涉及用于进行核酸测序的试剂盒和试剂盒的用途。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。

公开(公告)号：WO2013067272A1

公开(公告)日：2013-05-10

申请号：PCT/US2012/063190

申请日：2012-11-02

Applicant: DIAGNOSTICS FOR ALL, INC.

Inventor： ROLLAND, Jason ,  CONNELLY, John, Thomas

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/5023 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/04 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/126 ,  B01L2300/161 ,  C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2565/625

Abstract: The invention provides molecular diagnostic test devices and methods for using such diagnostic test devices to detect analytes of biological significance in a patient. The diagnostic test devices are particularly useful for detecting a polynucleotide analyte in a sample obtained from a patient. Further, the diagnostic test devices are inexpensive, disposable, easy to use, and are useful at the point of care.

Abstract translation: 本发明提供了使用这种诊断测试装置来检测患者生物学意义的分析物的分子诊断测试装置和方法。 诊断测试装置特别可用于检测从患者获得的样品中的多核苷酸分析物。 此外，诊断测试装置是便宜的，一次性的，易于使用的，并且在护理点是有用的。

公开(公告)号：WO2012086168A1

公开(公告)日：2012-06-28

申请号：PCT/JP2011/007064

申请日：2011-12-19

Applicant: 日本電気株式会社 ,  麻生川 稔 ,  萩原 久

Inventor： 麻生川　稔 ,  萩原　久

IPC: G01N35/00 ,  C12M1/00 ,  C12Q1/68 ,  G01N35/08 ,  G01N37/00

CPC classification number: F24H9/20 ,  B01L3/5027 ,  B01L3/52 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2400/0481 ,  G01N2035/00356

Abstract: 　マイクロチップを用いた試料の加熱方法において、少なくとも一部が弾性部材からなる容器部（７０ｅ）と、液体試料を容器部（７０ｅ）に導く流路（６１ｂ）と、を有するマイクロチップを用い、容器部（７０ｅ）に液体試料を収容した後、容器部（７０ｅ）の内部に対して圧力を加えながら、液体試料を加熱する。

Abstract translation: 提供一种使用微芯片的样品加热方法，其中所述方法采用微芯片，其包括：至少部分地由弹性构件形成的容器部分（70e）; 和用于将液体样品引导到容器部分（70e）的通道（61b）。 在液体样品已经被容纳在容器部分（70e）中之后，在将容器（70e）的内部施加压力的同时加热液体样品。