公开(公告)号：WO2010059232A1

公开(公告)日：2010-05-27

申请号：PCT/US2009/006221

申请日：2009-11-20

Applicant: BIOGEN IDEC MA INC. ,  SELVITELLI, Keith ,  MCCUE, Justin

Inventor： SELVITELLI, Keith ,  MCCUE, Justin

IPC: C12N7/06

CPC classification number: C12N7/00 ,  A61K39/21 ,  A61K39/245 ,  C07K1/14 ,  C07K14/005 ,  C12N7/06 ,  C12N2710/16063 ,  C12N2710/16763 ,  C12N2740/13063

Abstract: The present invention pertains to methods of using arginine to inactivate or reduce the infectious titer of enveloped viruses potentially present in biological compositions produced by eukaryotic cells (such as a antibodies or other therapeutic proteins). In some embodiments, inactivation or reduction of viral titers by exposure to arginine is achieved in a neutral (pH ~7) or near neutral (~pH 6 to ~pH 8) environment.

Abstract translation: 本发明涉及使用精氨酸灭活或降低可能存在于由真核细胞（例如抗体或其它治疗性蛋白质）产生的生物组合物中的包膜病毒的感染滴度的方法。 在一些实施方案中，在中性（pH〜7）或接近中性（〜pH 6〜〜pH 8）的环境中实现了通过暴露于精氨酸而灭活或降低病毒效价。

公开(公告)号：WO2005077529A1

公开(公告)日：2005-08-25

申请号：PCT/US2005/003295

申请日：2005-02-03

Applicant: MILLIPORE CORPORATION ,  DILEO, Anthony, J. ,  MCCUE, Justin ,  MOYA, Wilson ,  QUINONES-GARCIA, Igor ,  SOICE, Neil, P. ,  THOM, Volkmar ,  YUAN, Sarah

Inventor： DILEO, Anthony, J. ,  MCCUE, Justin ,  MOYA, Wilson ,  QUINONES-GARCIA, Igor ,  SOICE, Neil, P. ,  THOM, Volkmar ,  YUAN, Sarah

IPC: B01J20/32

CPC classification number: B01J20/286 ,  B01J20/262 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28042 ,  B01J20/28078 ,  B01J20/28085 ,  B01J20/3035 ,  B01J20/305 ,  B01J20/321 ,  B01J20/3251 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J2220/62 ,  B01J2220/82

Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive coatings comprising from about 1 to about 80 % of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be crosslinked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.

Abstract translation: 能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附性涂层的多孔基材，其包含基材孔隙体积的约1至约80％。 所得到的底物具有良好的对流和扩散流量和容量。 底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

公开(公告)号：WO2005077528A1

公开(公告)日：2005-08-25

申请号：PCT/US2005/003281

申请日：2005-02-03

Applicant: MILLIPORE CORPORATION ,  DILEO, Anthony ,  MCCUE, Justin ,  MOYA, Wilson ,  QUINONES-GARCIA, Igor ,  SOICE, Neil, P. ,  THOM, Volkmar ,  YUAN, Sarah

Inventor： DILEO, Anthony ,  MCCUE, Justin ,  MOYA, Wilson ,  QUINONES-GARCIA, Igor ,  SOICE, Neil, P. ,  THOM, Volkmar ,  YUAN, Sarah

IPC: B01J20/32

CPC classification number: B01D15/20 ,  B01D15/265 ,  B01D15/361 ,  B01J20/26 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28042 ,  B01J20/28078 ,  B01J20/28097 ,  B01J20/286 ,  B01J20/3064 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/62

Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtratin of desired species including biomolecules such as proteins and DNA fragments.

Abstract translation: 能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附聚合物涂层的多孔基底，其包含基底孔隙体积的约1至约80％。 所得到的底物具有良好的对流和扩散流量和容量。 底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子如蛋白质和DNA片段）的色谱介质。

公开(公告)号：WO2009111347A1

公开(公告)日：2009-09-11

申请号：PCT/US2009/035581

申请日：2009-02-27

Applicant: BIOGEN IDEC MA INC. ,  MCCUE, Justin ,  ROMERO, Jonathan ,  MEIER, Werner ,  NOTARNICOLA, Stephen ,  EVANS, David ,  MACNIVEN, Richard

Inventor： MCCUE, Justin ,  ROMERO, Jonathan ,  MEIER, Werner ,  NOTARNICOLA, Stephen ,  EVANS, David ,  MACNIVEN, Richard

IPC: C07K14/715 ,  C07K1/20 ,  C12N15/62 ,  C07K1/16

CPC classification number: C07K14/7151 ,  A61K47/6811 ,  A61K47/6835 ,  C07K2319/30 ,  C07K2319/32

Abstract: The invention provides methods and compositions for separating impurities during the manufacture of immunoglobulin (Ig) fusion proteins. Examples of impurities which may be removed in accordance with the methods of the invention include inactive forms of the Ig fusion protein and/or aggregates.

Abstract translation: 本发明提供了在制造免疫球蛋白（Ig）融合蛋白期间分离杂质的方法和组合物。 根据本发明的方法可以除去的杂质的实例包括Ig融合蛋白和/或聚集体的无活性形式。