-
公开(公告)号：WO2008153733A3
公开(公告)日：2009-05-14
申请号：PCT/US2008006554
申请日：2008-05-22
发明人： WILLIAMS JAMES A
CPC分类号： A61K48/0066 , A61K39/12 , A61K39/145 , A61K48/00 , A61K2039/53 , A61K2039/55561 , C07K14/005 , C12N15/111 , C12N15/113 , C12N15/117 , C12N15/85 , C12N2310/11 , C12N2310/111 , C12N2310/17 , C12N2310/532 , C12N2320/30 , C12N2320/50 , C12N2710/10322 , C12N2760/16122 , C12N2760/16134 , C12N2800/106 , C12N2800/107 , C12N2830/00
摘要： Improved DNA vaccine plasmids are disclosed that contain novel immunostimulatory RNA compositions. The improved plasmids eliminate all extraneous sequences, incorporate a novel antibiotic free short RNA based selectable marker, increase eukaryotic expression using a novel chimeric promoter, improve yield and stability during bacterial production, and improve immunostimulation. These vectors are utilized in immunization to elicit improved immune responses or therapy to induce type 1 interferon production.
摘要翻译： 公开了包含新型免疫刺激RNA组合物的改进的DNA疫苗质粒。 改进的质粒消除所有外来序列，并结合一种新型的基于抗生素的短基于RNA的可选择标记，使用新型嵌合启动子增加真核表达，提高细菌生产过程中的产量和稳定性，并改善免疫刺激。 这些载体用于免疫以引发改善的免疫应答或诱导1型干扰素生产的治疗。
-
2.发明申请
公开(公告)号：WO2006081529A3
公开(公告)日：2007-03-29
申请号：PCT/US2006003148
申请日：2006-01-27
发明人： WILLIAMS JAMES A
IPC分类号： G06F19/00
摘要： Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity. These processes feature RNA based inducers of plasmid copy number.
摘要翻译： 需要改进质粒DNA生产技术，以确保未来DNA疫苗和DNA治疗的经济可行性。 描述了一般方法，通过这些方法可以显着提高质粒DNA的生产力。 这些过程具有质粒拷贝数的基于RNA的诱导物。
-
3.发明申请
公开(公告)号：WO2006081529A2
公开(公告)日：2006-08-03
申请号：PCT/US2006/003148
申请日：2006-01-27
发明人： WILLIAMS, James, A.
IPC分类号： C12Q1/68
摘要： Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity. These processes feature RNA based inducers of plasmid copy number.
摘要翻译： 需要改进质粒DNA生产技术，以确保未来DNA疫苗和DNA治疗的经济可行性。 描述了一般方法，通过这些方法可以显着提高质粒DNA的生产力。 这些过程具有质粒拷贝数的基于RNA的诱导物。
-
公开(公告)号：WO2008153733A8
公开(公告)日：2016-07-14
申请号：PCT/US2008006554
申请日：2008-05-22
发明人： WILLIAMS JAMES A
CPC分类号： A61K48/0066 , A61K39/12 , A61K39/145 , A61K48/00 , A61K2039/53 , A61K2039/55561 , C07K14/005 , C12N15/111 , C12N15/113 , C12N15/117 , C12N15/85 , C12N2310/11 , C12N2310/111 , C12N2310/17 , C12N2310/532 , C12N2320/30 , C12N2320/50 , C12N2710/10322 , C12N2760/16122 , C12N2760/16134 , C12N2800/106 , C12N2800/107 , C12N2830/00
摘要： Improved DNA vaccine plasmids are disclosed that contain novel immunostimulatory RNA compositions. The improved plasmids eliminate all extraneous sequences, incorporate a novel antibiotic free short RNA based selectable marker, increase eukaryotic expression using a novel chimeric promoter, improve yield and stability during bacterial production, and improve immunostimulation. These vectors are utilized in immunization to elicit improved immune responses or therapy to induce type 1 interferon production.
摘要翻译： 公开了包含新型免疫刺激RNA组合物的改进的DNA疫苗质粒。 改进的质粒消除所有外来序列，并结合一种新型的基于抗生素的短基于RNA的可选择标记，使用新的嵌合启动子增加真核表达，提高细菌生产过程中的产量和稳定性，并改善免疫刺激。 这些载体用于免疫以引发改善的免疫应答或诱导1型干扰素生产的治疗。
-
公开(公告)号：WO2006078979A4
公开(公告)日：2009-11-05
申请号：PCT/US2006002174
申请日：2006-01-20
发明人： WILLIAMS JAMES A
CPC分类号： A61K39/00 , A61K2039/53 , C12N15/85 , C12N2800/106 , C12N2800/107 , C12N2830/15
摘要： Improved DNA vaccine plasmids are disclosed. The improved plasmids eliminate all extraneous sequences, and have superior eukaryotic expression, improved yield and stability during bacterial production, facilitate flexible targeting of antigens to various intracellular destinations, and novel RNA-based functionality. These vectors are utilized in novel immunization methods wherein combinations of immunostimulatory DNA vaccine plasmids that target antigens to various intracellular destinations are used to elicit improved immune response.
摘要翻译： 公开了改进的DNA疫苗质粒。 改进的质粒消除所有外来序列，并且在细菌生产期间具有优异的真核表达，提高的产量和稳定性，促进抗原到各种细胞内目的地的灵活靶向以及新的基于RNA的功能。 这些载体用于新型免疫方法中，其中使用将抗原靶向各种细胞内目的地的免疫刺激DNA疫苗质粒的组合引发改善的免疫应答。
-
公开(公告)号：WO2008153731A4
公开(公告)日：2009-02-26
申请号：PCT/US2008006548
申请日：2008-05-22
发明人： WILLIAMS JAMES A
CPC分类号： C12N15/902 , C12N15/1079
摘要： Improvements in strain engineering technology are needed to insure the economic feasibility of future engineered recombinant organisms for industrial biotechnology. Disclosed herein are rapid, efficient methods (Genome Mass Transfer) that facilitate introduction of new selectable traits into a target microbial host. In one preferred embodiment, methods for high efficiency electroporation mediated transfer of donor DNA into a recipient microbial cell are disclosed.
摘要翻译： 需要改进应变工程技术，以确保未来工程重组生物工业生物技术的经济可行性。 本文公开了有助于将新的可选性状引入目标微生物宿主中的快速，有效的方法（Genome Mass Transfer）。 在一个优选的实施方案中，公开了用于高效电穿孔介导的供体DNA转移到受体微生物细胞中的方法。
-
公开(公告)号：WO2006078979A2
公开(公告)日：2006-07-27
申请号：PCT/US2006/002174
申请日：2006-01-20
发明人： WILLIAMS, James, A.
CPC分类号： A61K39/00 , A61K2039/53 , C12N15/85 , C12N2800/106 , C12N2800/107 , C12N2830/15
摘要： Improved DNA vaccine plasmids are disclosed. The improved plasmids eliminate all extraneous sequences, and have superior eukaryotic expression, improved yield and stability during bacterial production, facilitate flexible targeting of antigens to various intracellular destinations, and novel RNA-based functionality. These vectors are utilized in novel immunization methods wherein combinations of immunostimulatory DNA vaccine plasmids that target antigens to various intracellular destinations are used to elicit improved immune response.
摘要翻译： 公开了改进的DNA疫苗质粒。 改进的质粒消除所有外来序列，并且在细菌生产期间具有优异的真核表达，提高的产量和稳定性，促进抗原到各种细胞内目的地的灵活靶向以及新的基于RNA的功能。 这些载体用于新型免疫方法中，其中使用将抗原靶向各种细胞内目的地的免疫刺激DNA疫苗质粒的组合引发改善的免疫应答。
-
公开(公告)号：WO2006023546A2
公开(公告)日：2006-03-02
申请号：PCT/US2005/029238
申请日：2005-08-16
IPC分类号： C12N15/74
CPC分类号： A61K48/0091 , C12N15/69 , C12N15/70 , C12N15/74 , C12P19/34
摘要： Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.
摘要翻译： 需要改进质粒DNA生产技术，以确保未来DNA疫苗和DNA治疗的经济可行性。 描述了通用方法，通过它们可以显着提高发酵罐中的质粒DNA生产力。 这些方法具有补料分批发酵策略，结合新生长和诱导相温度变化。
-
公开(公告)号：WO2009025690A3
公开(公告)日：2009-11-12
申请号：PCT/US2008006553
申请日：2008-05-22
CPC分类号： C12N15/64 , C12N1/06 , C12N15/1003 , C12N15/1017 , C12N15/73
摘要： General methods and strains of bacteria are described, that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.
摘要翻译： 描述了细菌的一般方法和菌株，其大大简化和简化了质粒DNA的生产。 在一个优选的实施方案中，细胞内溶素介导的质粒提取结合絮凝介导的细胞碎片和宿主核酸的去除通过简化的下游纯化和减少的废物流实现提高的产量和纯度，从而降低生产成本。
-
公开(公告)号：WO2006078979A9
公开(公告)日：2009-09-17
申请号：PCT/US2006002174
申请日：2006-01-20
发明人： WILLIAMS JAMES A
CPC分类号： A61K39/00 , A61K2039/53 , C12N15/85 , C12N2800/106 , C12N2800/107 , C12N2830/15
摘要： Improved DNA vaccine plasmids are disclosed. The improved plasmids eliminate all extraneous sequences, and have superior eukaryotic expression, improved yield and stability during bacterial production, facilitate flexible targeting of antigens to various intracellular destinations, and novel RNA-based functionality. These vectors are utilized in novel immunization methods wherein combinations of immunostimulatory DNA vaccine plasmids that target antigens to various intracellular destinations are used to elicit improved immune response.
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.01 秒)
