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公开(公告)号：WO2012061442A1
公开(公告)日：2012-05-10
申请号：PCT/US2011/058854
申请日：2011-11-01
Applicant: NESS, Kevin, D. , HIDDESSEN, Amy, L. , WYATT, Paul, W.
Inventor： NESS, Kevin, D. , HIDDESSEN, Amy, L. , WYATT, Paul, W.
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q2527/146 , C12Q2563/159
Abstract: Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof.
Abstract translation: 分析基因组DNA的方法 可以获得包括靶标的基因组DNA。 基因组DNA可能会被片段化以产生片段化的DNA。 碎片DNA可以通过液滴发生器产生含有片段DNA的水滴。 可以对液滴进行测定以确定靶标的水平。 在一些实施方案中，液滴可以含有至少约5纳克每微升浓度的基因组DNA，所述液滴可以以每秒至少约50个液滴的液滴产生频率产生，所述液滴可以具有 每滴小于约10纳升，可以以大于约50纳升/秒的流速或其任何组合产生液滴。
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公开(公告)号：WO2011120006A8
公开(公告)日：2011-12-15
申请号：PCT/US2011030077
申请日：2011-03-25
Applicant: QUANTALIFE INC , NESS KEVIN D , ARBORE MARK A , HURST JERRY E , KLEIN DAVID L , MASQUELIER DONALD A
Inventor： NESS KEVIN D , ARBORE MARK A , HURST JERRY E , KLEIN DAVID L , MASQUELIER DONALD A
IPC: C12M1/34
CPC classification number: G01N21/6428 , B01L3/502784 , G01N15/1456 , G01N21/05 , G01N21/31 , G01N21/553 , G01N21/645 , G01N21/6486 , G01N2021/0346 , G01N2021/052 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/8564 , G01N2201/062 , G01N2201/0627 , G01N2201/0696
Abstract: System, including methods and apparatus, for light detection and signal processing for droplet-based assays.
Abstract translation: 用于基于液滴的测定的光检测和信号处理的系统，包括方法和装置。
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公开(公告)号：WO2011120024A1
公开(公告)日：2011-09-29
申请号：PCT/US2011/030101
申请日：2011-03-25
Applicant: QUANTALIFE, INC. , NESS, Kevin, D. , KELLY, Christopher, F. , MASQUELIER, Donald, A.
Inventor： NESS, Kevin, D. , KELLY, Christopher, F. , MASQUELIER, Donald, A.
IPC: G01N33/00
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01L3/0275 , B01L3/502715 , B01L3/502723 , B01L7/52 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2400/0487 , C12Q1/6806 , G01N35/085 , G01N35/1065 , G01N2035/00148 , G01N2035/1034 , Y10T29/494
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample- containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
Abstract translation: 一种用于产生适合于基于液滴的测定的液滴的方法和装置的系统。 所公开的系统可以包括通过将含水样品的流体与背景乳液流体（例如油）并入而形成含有样品的液滴的乳液，所述液体产生组件被配置成通过将含水样品的流体与背景乳液流体 在背景液中。 在一些情况下，所公开的系统可以包括被配置成将含样品的液滴输送到出口区域的通道或其它合适的机制，从而可以执行随后的测定步骤。
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Patent Agency Ranking
公开(公告)号：WO2011120006A1
公开(公告)日：2011-09-29
申请号：PCT/US2011/030077
申请日：2011-03-25
Applicant: AUANTALIFE, INC. A DELAWARE CORPORATION , NESS, Kevin, D. , ARBORE, Mark, A. , HURST, Jerry, E. , KLEIN, David, L. , MASQUELIER, Donald, A.
Inventor： NESS, Kevin, D. , ARBORE, Mark, A. , HURST, Jerry, E. , KLEIN, David, L. , MASQUELIER, Donald, A.
IPC: C12M1/34
CPC classification number: G01N21/6428 , B01L3/502784 , G01N15/1456 , G01N21/05 , G01N21/31 , G01N21/553 , G01N21/645 , G01N21/6486 , G01N2021/0346 , G01N2021/052 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/8564 , G01N2201/062 , G01N2201/0627 , G01N2201/0696
Abstract: System, including methods and apparatus, for light detection and signal processing for droplet-based assays.
Abstract translation: 包括用于基于液滴的测定法的光检测和信号处理的系统，包括方法和装置。
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公开(公告)号：WO2012061444A2
公开(公告)日：2012-05-10
申请号：PCT/US2011058857
申请日：2011-11-01
Applicant: HIDDESSEN AMY L , MASQUELIER DONALD A , NESS KEVIN D , HINDSON BENJAMIN J , MAKAREWICZ ANTHONY J JR , STEENBLOCK ERIN R
Inventor： HIDDESSEN AMY L , MASQUELIER DONALD A , NESS KEVIN D , HINDSON BENJAMIN J , MAKAREWICZ ANTHONY J JR , STEENBLOCK ERIN R
CPC classification number: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F13/1022 , B01L3/502761 , B01L2200/0605 , B01L2200/0673 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2035/1046 , Y10T137/0396 , Y10T137/8593
Abstract: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.
Abstract translation: 系统，包括用于形成乳液的方法，设备和试剂盒。 该系统可以包括由仪器接收的仪器和微流体芯片。 该仪器可以对由芯片保持的预期乳剂阶段施加压力，以驱动芯片中乳剂的形成和收集。 在一些实施例中，当仪器检测到满足预定条件的压力变化时，仪器可以停止对芯片施加压力。 改变可能表明已经达到了液滴产生的终点。
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公开(公告)号：WO2011120020A1
公开(公告)日：2011-09-29
申请号：PCT/US2011/030097
申请日：2011-03-25
Applicant: QUANTALIFE, INC. , NESS, Kevin, D. , HINDSON, Benjamin, J. , MAKAREWICZ, Anthony, J. , HIDDESSEN, Amy, L.
CPC classification number: B01L3/021 , B01L2200/0673 , B01L2400/0478 , B01L2400/0622
Abstract: System, including methods and apparatus, for transporting droplets from a tip to an examination site for detection.
Abstract translation: 用于将液滴从尖端输送到检查部位以进行检测的系统，包括方法和装置。
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公开(公告)号：WO2011028539A1
公开(公告)日：2011-03-10
申请号：PCT/US2010/046519
申请日：2010-08-24
Applicant: QUANTALIFE, INC. , HINDSON, Benjamin, J. , COLSTON, Billy, W. Jr. , NESS, Kevin, D. , MASQUELIER, Donald, A.
IPC: C12Q1/00
CPC classification number: B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
Abstract translation: 系统，包括用于通过多个乳液的聚结混合小体积的流体的方法，装置，组合物和试剂盒。
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公开(公告)号：WO2012061444A3
公开(公告)日：2012-05-10
申请号：PCT/US2011/058857
申请日：2011-11-01
Applicant: HIDDESSEN, Amy L. , MASQUELIER, Donald A. , NESS, Kevin D. , HINDSON, Benjamin J. , MAKAREWICZ, Anthony J., Jr. , STEENBLOCK, Erin R.
Inventor： HIDDESSEN, Amy L. , MASQUELIER, Donald A. , NESS, Kevin D. , HINDSON, Benjamin J. , MAKAREWICZ, Anthony J., Jr. , STEENBLOCK, Erin R.
IPC: B01F15/00
Abstract: System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.
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