公开(公告)号：WO2012134262A2

公开(公告)日：2012-10-04

申请号：PCT/MY2012000044

申请日：2012-03-07

Applicant: UNIVERSITI PUTRA MALAYSIA U P M ,  HAJI AHMAD ZALINAH ,  RASTEGARI ELHAM ,  SPENCER DAVID FARRINGDON ,  ISMAIL MAZNAH

Inventor： HAJI AHMAD ZALINAH ,  RASTEGARI ELHAM ,  SPENCER DAVID FARRINGDON ,  ISMAIL MAZNAH

IPC: C07K14/415 ,  C07K1/12 ,  C12P21/06 ,  G01N33/68

CPC classification number: C07K1/145 ,  C07K1/128 ,  C07K14/415 ,  C12Y302/01015 ,  C12Y402/0201 ,  G01N33/6803

Abstract: The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.

Abstract translation: 本发明涉及从植物材料中提取蛋白质的方法，其特征在于高含量的干扰化合物，特别是丰富的多糖如果胶。 本发明的方法在蛋白质提取之前在植物材料样品的制备中应用果胶溶解处理。 这种优化的方法导致高质量的蛋白质提取物与进一步敏感的蛋白质组学分析相适应，具体方法如二维凝胶电泳或质谱法。 本发明还涉及通过本发明方法获得的蛋白质提取物和本发明方法在制备蛋白质样品中的用途。 公开了用于进行本发明方法的蛋白质提取试剂盒，以及蛋白质提取试剂盒在蛋白质分析和蛋白质组学中的用途。

公开(公告)号：WO2012134262A3

公开(公告)日：2012-10-04

申请号：PCT/MY2012/000044

申请日：2012-03-07

Applicant: UNIVERSITI PUTRA MALAYSIA (U.P.M.) ,  HAJI.AHMAD, Zalinah ,  RASTEGARI, Elham ,  SPENCER, David, Farringdon ,  ISMAIL, Maznah

Inventor： HAJI.AHMAD, Zalinah ,  RASTEGARI, Elham ,  SPENCER, David, Farringdon ,  ISMAIL, Maznah

IPC: C07K14/415 ,  C07K1/12 ,  C12P21/06 ,  G01N33/68

Abstract: The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.