公开(公告)号：WO2012134262A2

公开(公告)日：2012-10-04

申请号：PCT/MY2012000044

申请日：2012-03-07

Applicant: UNIVERSITI PUTRA MALAYSIA U P M ,  HAJI AHMAD ZALINAH ,  RASTEGARI ELHAM ,  SPENCER DAVID FARRINGDON ,  ISMAIL MAZNAH

Inventor： HAJI AHMAD ZALINAH ,  RASTEGARI ELHAM ,  SPENCER DAVID FARRINGDON ,  ISMAIL MAZNAH

IPC: C07K14/415 ,  C07K1/12 ,  C12P21/06 ,  G01N33/68

CPC classification number: C07K1/145 ,  C07K1/128 ,  C07K14/415 ,  C12Y302/01015 ,  C12Y402/0201 ,  G01N33/6803

Abstract: The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.

Abstract translation: 本发明涉及从植物材料中提取蛋白质的方法，其特征在于高含量的干扰化合物，特别是丰富的多糖如果胶。 本发明的方法在蛋白质提取之前在植物材料样品的制备中应用果胶溶解处理。 这种优化的方法导致高质量的蛋白质提取物与进一步敏感的蛋白质组学分析相适应，具体方法如二维凝胶电泳或质谱法。 本发明还涉及通过本发明方法获得的蛋白质提取物和本发明方法在制备蛋白质样品中的用途。 公开了用于进行本发明方法的蛋白质提取试剂盒，以及蛋白质提取试剂盒在蛋白质分析和蛋白质组学中的用途。

公开(公告)号：WO2013006034A3

公开(公告)日：2013-03-14

申请号：PCT/MY2012000196

申请日：2012-07-02

Applicant: UNIVERSITI PUTRA MALAYSIA U P M ,  BAHAMAN ABDUL RANI ,  SEENICHAMY ARIVUDAINAMBI

Inventor： BAHAMAN ABDUL RANI ,  SEENICHAMY ARIVUDAINAMBI

IPC: G01N33/569 ,  C12Q1/04

CPC classification number: G01N33/56911 ,  C07K16/1207 ,  C07K2317/20 ,  G01N2333/20

Abstract: The present invention relates to a diagnostic method for the detection of acute leptospirosis. The method of the invention allows for a quick, specific and sensitive detection of the disease caused by pathogenic Leptospira. The described method is based on the immunological detection of the protein LipL21, a membrane protein expressed specifically by pathogenic strains of Leptospira. Furthermore, the present invention relates to the development of a diagnostic device that allows positive diagnosis to be conducted in the early and critical stage of the infection. The preferred diagnostic device of the invention is a lateral flow test, which as a hand-held assay, is easy to use and facilitates diagnosis of leptospirosis in the first 5 days of infection. Further disclosed is the antibody raised against the LipL21 protein as well as its respective uses in the diagnostic methods and devices. Finally, a diagnostic kit is disclosed containing the inventive materials described herein to conduct diagnosis of acute leptospirosis during the critical early stage of infection. The first 5 days of the infection is where the subject or patient could succumb to the disease. Diagnosis of the disease at this critical stage of infection would then advise the affected patient to have immediate systemic antibiotic treatment and thereby, avoid succumbing to the disease.

Abstract translation: 本发明涉及用于检测急性钩端螺旋体病的诊断方法。 本发明的方法允许对由病原性钩端螺旋体引起的疾病进行快速，特异和灵敏的检测。 所描述的方法基于蛋白质LipL21的免疫学检测，LipL21是由钩端螺旋体致病性菌株特异性表达的膜蛋白。 此外，本发明涉及诊断装置的开发，其允许在感染的早期和关键阶段进行阳性诊断。 本发明的优选诊断装置是横向流动测试，其作为手持测定法易于使用并且便于在感染的前5天诊断钩端螺旋体病。 还公开了针对LipL21蛋白产生的抗体以及其在诊断方法和装置中的各自用途。 最后，公开了一种诊断试剂盒，其包含本文所述的本发明材料以在感染的关键早期阶段进行急性钩端螺旋体病的诊断。 感染的前5天是受试者或患者可能屈服于该疾病的地方。 在感染的这个关键阶段对疾病的诊断将建议受影响的患者立即进行全身抗生素治疗，从而避免疾病的死亡。