公开(公告)号：WO2021170868A1

公开(公告)日：2021-09-02

申请号：PCT/EP2021/055008

申请日：2021-03-01

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： KALISVAART, Jonathan ,  FRIJTERS, Raoul Jacobus Johannes Maria ,  LUDEKING, Daniël Johannes Wilhelmus ,  ROOVERS, Alwin Johannes Marinus

IPC: C07K14/415 ,  C12N15/82 ,  A01H5/00

Abstract: The present invention relates to a modified Albino3-like gene the wildtype of which comprises SEQ ID No. 1 encoding SEQ ID No. 2, or a homologous sequence having at least 70% sequence identity to SEQ ID No. 1, which modified gene leads to resistance to a Tobamovirus, preferably to ToBRFV. The modified Albino3-like gene preferably encodes an Albino3-like protein 2 according to SEQ ID No. 6 or SEQ ID No. 7, or encodes an Albino3-like protein 2 having at least 70% sequence identity thereto. Examples of modifications comprise a C485G SNP, an A1300G SNP and/or a T1381C SNP, or a SNP on a corresponding position of a homologous sequence. The invention further relates to plants comprising the modified gene and showing resistance to a Tobamovirus.

公开(公告)号：WO2021064118A1

公开(公告)日：2021-04-08

申请号：PCT/EP2020/077558

申请日：2020-10-01

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： BRACUTO, Valentina ,  KOEKEN, Adrianus Cornelis ,  MULLER, Florian ,  VILLADA, Emilio Sarria

IPC: C12N15/82 ,  C07K14/415 ,  A01H5/00

Abstract: The present invention relates to a modified PriL gene, which encodes a modified protein comprising one or more modifications in the wild type protein sequence of SEQ ID No. 1 or SEQ ID No. 3 or in a protein sequence having at least 60% sequence identity to SEQ ID No. 1 or SEQ ID No. 3. Alternatively, the wild type of the modified PriL gene encodes a protein according to SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 7, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, SEQ ID No. 29, or SEQ ID No. 31. The modification in the wild type protein sequence is an amino acid substitution, in particular a phenylalanine (F) to cysteine (C) substitution at a position corresponding to position 147 according to SEQ ID No. 5, and/or glycine (G) to aspartic acid (D) substitution at a position corresponding to position 162 according to SEQ ID No. 5, and/or a glutamine (Q) to histidine (H) substitution at a position corresponding to position 163 according to SEQ ID No. 5, and/or wherein the modified gene encodes a modified protein that comprises a tyrosine (Y) to histidine (H) substitution at a position corresponding to position 4 according to SEQ ID No. 9, and/or a proline (P) to glutamine (Q) substitution at a position corresponding to position 166 according to SEQ ID No. 9, and/or an isoleucine (I) to threonine (T) substitution at a position corresponding to position 200 according to SEQ ID No. 9.

公开(公告)号：WO2018060445A1

公开(公告)日：2018-04-05

申请号：PCT/EP2017/074810

申请日：2017-09-29

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： KOCK, Vincent Laurens Adrianus ,  FEITSMA, Johannes Geert Jan ,  FRIJTERS, Raoul Jacobus Johannes Maria

IPC: A01H5/12 ,  C07K14/415 ,  C12N15/82

CPC classification number: A01H5/12 ,  C07K14/415 ,  C12N15/8282

Abstract: The present invention relates to an allele designated alpha-WOLF 8 which confers resistance to at least one Peronospora farinosa f. Sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif "MAEIGYSVC" at its N-terminus; and b) the motif "KWMCLR"; and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:12. When the allele is homozygously present in a spinach plant confers complete resistance to Peronospora farinosa f. Sp. spinacea races pfs:1, pfs:2, pfs:6, pfs:8 and pfs:15, and isolate US1508, and confers intermediate resistance to pfs:5, pfs:10 and pfs:16, and does not confer resistance to pfs:3, pfs:4, pfs:7, pfs:9, pfs:11, pfs:12, pfs:13 and pfs:14.

Abstract translation: 本发明涉及称为α-WOLF8的等位基因，其赋予对至少一种霜霉病f的抗性。 SP。 其中由所述等位基因编码的蛋白质是CC-NBS-LRR蛋白质，其在其氨基酸序列中包含：a）基序“MAEIGYSVC” 在其N端; 和b）基序“KWMCLR”; 并且其中所述蛋白质的LRR结构域按照与SEQ ID No：12至少92％，93％，94％，95％，96％，97％，98％，99％，100％ 。 当等位基因纯合地存在于菠菜植物中时，赋予对Peronospora farinosa f的完全抗性。 SP。 spinacea races pfs：1，pfs：2，pfs：6，pfs：8和pfs：15，并且隔离US1508，并且赋予对pfs：5，pfs：10和pfs：16的中等抗性，并且不赋予对pfs的抗性 ：3，pfs：4，pfs：7，pfs：9，pfs：11，pfs：12，pfs：13和pfs：14。

公开(公告)号：WO2017144669A1

公开(公告)日：2017-08-31

申请号：PCT/EP2017/054343

申请日：2017-02-24

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： VAN DUN, Cornelis Maria Petrus ,  SCHUT, Johannes Wilhelmus ,  DE NOOIJER, Silvester ,  DE ROOIJ, Johannis Hendrik

IPC: A01H5/12 ,  C12N9/02 ,  C12N15/82 ,  C12Q1/68

CPC classification number: A01H5/12 ,  C12N9/0073 ,  C12N15/8271 ,  C12Q1/68

Abstract: The present invention relates a plant comprising a modified F5H gene homolog, wherein said gene homolog comprises a modification as compared to its corresponding wild type F5H gene homolog, wherein the presence of the modified F5H gene homolog in the plant leads to a reduction of wound-induced surface discoloration in comparison to a plant not comprising the modified F5H gene homolog. The invention also relates to a modified F5H gene homolog that leads to the reduced wound-induced surface discoloration. The invention further relates to use of the gene in breeding and producing plants that show reduced wound-induced surface discoloration.

Abstract translation: 本发明涉及包含修饰的F5H基因同源物的植物，其中所述基因同源物与其相应的野生型F5H基因同源物包含修饰， 基因同系物，其中与未包含修饰的F5H基因的植物相比，植物中修饰的F5H基因同源物的存在导致伤口诱导的表面变色的减少 同源。 本发明还涉及导致伤口诱导的表面变色减少的修饰的F5H基因同系物。 本发明还涉及该基因在培育和生产显示减少的伤口诱导的表面变色的植物中的用途。

公开(公告)号：WO2017072300A1

公开(公告)日：2017-05-04

申请号：PCT/EP2016/076079

申请日：2016-10-28

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： SOLLEVELD, Johan Cornelis ,  NOWOSIELSKI, Agathe Anna ,  KOOPMANS, Roy

IPC: A01H5/08 ,  C12Q1/68

CPC classification number: A01H5/08 ,  A01H1/04 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: The present invention relates to a tomato plant which carries at least one QTL in its genome that leads to its fruits comprising higher levels of anthocyanins when compared to fruits produced by a tomato plant not carrying said QTL in its genome, wherein said fruits are not purple at the red-ripe harvest stage. The QTL may be QTL1 which is located on chromosome 10 between marker sequence SEQ ID No. 1 and the end of said chromosome and can be identified by the markers having SEQ ID No. 2 and SEQ ID No. 3. Optionally, also QTL2 is present, which is located on chromosome 9 between marker sequences SEQ ID No. 4 and SEQ ID No. 5 and which can be identified by the marker having SEQ ID No. 9 and/or SEQ ID No. 22. Optionally, also QTL3 is present, which is located on chromosome 7 between marker sequences SEQ ID No. 13 and SEQ ID No. 14 and which can be identified by the markers having SEQ ID Nos. 15-20. QTL1 and optionally QTL2 and/or QTL3 are as comprised in the genome of a tomato plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42470. A tomato plant of the invention can also comprise all QTLs, each either in homozygous or heterozygous form. The invention further relates to progeny of the plant, propagation material for the plant and to markers for identifying the QTLs and their use.

Abstract translation: 番茄植物本发明涉及在其基因组中携带至少一个QTL的番茄植物，与其在其基因组中携带所述QTL的番茄植物产生的果实相比导致其果实包含更高水平的花青素 其中所述果实在红熟期收获阶段不是紫色的。 QTL可以是位于第10号染色体上位于标记序列SEQ ID No.1和所述染色体末端之间的QTL1，并且可以由具有SEQ ID No.2和SEQ ID No.3的标记鉴定。任选地，QTL2也是 存在于位于染色体9上的标记序列SEQ ID No.4和SEQ ID No.5之间，并且可以通过具有SEQ ID No.9和/或SEQ ID No.22的标记鉴定。任选地，QTL3也是 存在于位于第7号染色体上的标记序列SEQ ID No.13和SEQ ID No.14之间，并且可以通过具有SEQ ID No.15-20的标记鉴定。 QTL1和任选的QTL2和/或QTL3包含在番茄植物代表性种子的基因组中，所述番茄植物代表性种子以保藏编号NCIMB 42470保藏在NCIMB中。本发明的番茄植物还可以包含所有QTL，每个QTL均为纯合的或 杂合形式。 本发明进一步涉及植物的后代，植物的繁殖材料和用于鉴定QTL及其用途的标记。

公开(公告)号：WO2016195482A1

公开(公告)日：2016-12-08

申请号：PCT/NL2016/050264

申请日：2016-04-14

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： BOVÉE, Adrianus Johannes Cornelis

IPC: A01C21/00 ,  A01C7/04 ,  G06K9/48 ,  B25J9/16

CPC classification number: A01G9/00 ,  A01C7/04 ,  A01C14/00 ,  A01C21/005 ,  A01G9/085 ,  A01G22/00 ,  B07C5/02 ,  B07C5/3422 ,  B07C5/3425 ,  B07C5/36 ,  B07C5/365 ,  B25J9/1679 ,  B25J9/1697 ,  G06K9/00664

Abstract: The present invention relates to a device and method for sowing seeds. The invention relates more particularly to a device and method for sowing seeds as part of a plant. The system according to the invention comprises a separating device for separating a single seed relative to a remainder of the plurality of seeds, an optical recognition system (9) for recognizing the separated seed, a robot arm device (4) for picking up the separated seed and for sowing the picked-up seed, arid a control unit for controlling the separating device, the optical recognition system (9) and the robot arm device (4). With this system it is possible to sow seeds in a more accurate and less error-prone manner.

Abstract translation: 本发明涉及播种种子的装置和方法。 本发明更具体地涉及将种子播种为植物的一部分的装置和方法。 根据本发明的系统包括用于分离单个种子相对于多个种子中的其余部分的分离装置，用于识别分离的种子的光学识别系统（9），用于拾取分离的种子的机器人手臂装置（4） 种子和用于播种拾取的种子，以及用于控制分离装置的控制单元，光学识别系统（9）和机器人手臂装置（4）。 利用该系统，可以以更精确和更不容易出错的方式播种种子。

公开(公告)号：WO2015152723A1

公开(公告)日：2015-10-08

申请号：PCT/NL2015/050215

申请日：2015-04-02

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： BOVÉE, Adrianus Johannes Cornelis

IPC: G01N1/08

CPC classification number: G01N1/08 ,  G01N1/286 ,  G01N2001/2873 ,  G01N2001/288

Abstract: The present invention is related to a cutting system. It is further related to a system for obtaining a sample of plant material and method. Such a system can for instance be used for DNA analysis as part of a plant breeding program. According to the invention, the cutting system comprises a cutting device having a hollow cutting element having a cutting edge facing a leaf of a plant to be sampled, a drive unit for rotating said cutting element about a longitudinal axis of said cutting element to cut through the leaf, and a gas flow direction unit for applying a suction force through the hollow cutting element. The cutting system further comprises a control unit for controlling the drive unit and the gas flow direction unit. According to the invention, the hollow cutting element is elongated and the control unit is configured to control the gas flow direction unit and the drive unit such that said suction force is applied during said cutting through the leaf, and preferably at least prior to said cutting through the leaf.

Abstract translation: 本发明涉及一种切割系统。 进一步涉及获得植物材料和方法样品的系统。 这样的系统可以例如用于DNA分析，作为植物育种程序的一部分。 根据本发明，切割系统包括具有中空切割元件的切割装置，该中空切割元件具有面向要采样的植物的叶片的切割边缘，用于围绕所述切割元件的纵向轴线旋转所述切割元件以切割的驱动单元 叶片和用于通过中空切割元件施加吸力的气体流动方向单元。 切割系统还包括用于控制驱动单元和气体流动方向单元的控制单元。 根据本发明，中空切割元件是细长的，并且控制单元构造成控制气体流动方向单元和驱动单元，使得在所述切割期间通过叶片施加所述吸力，并且优选地至少在所述切割之前 通过叶子。

公开(公告)号：WO2015136085A1

公开(公告)日：2015-09-17

申请号：PCT/EP2015/055322

申请日：2015-03-13

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. ,  NUNHEMS B.V. ,  VILMORIN & CIE

Inventor： JEUKEN, Marie Jeanette Willemijn ,  DEN BOER, Erik ,  VISSER, Richard Gerardus Franciscus ,  NIKS, Rients Engelhard

IPC: A01H5/12

CPC classification number: A01H5/12 ,  A01H1/02 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The invention relates to a lettuce plant ( Lactuca sativa L .) comprising a resistance allele which confers a broad spectrum resistance to Bremia lactucae , wherein the resistance allele is located on linkage group 9, and which resistance allele is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB 42141. The invention further provides seeds, progeny, propagation material and food products from the plant.

Abstract translation: 本发明涉及一种莴苣植物（Lactuca sativa L.），其包含赋予对Bremia lactucae的广谱抗性的抗性等位基因，其中抗性等位基因位于连接群9上，并且抗性等位基因如在植物基因组中发现 从代表性样品以种子号NCIMB 42141保藏的种子生长。本发明还提供种子，后代，繁殖材料和来自植物的食品。

公开(公告)号：WO2014140209A1

公开(公告)日：2014-09-18

申请号：PCT/EP2014/055012

申请日：2014-03-13

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： DEN BRABER, Jan Hugo

IPC: A01H5/12 ,  A61K36/21

CPC classification number: A01H5/12

Abstract: The present invention relates to a spinach plant (Spinacia oleracea) comprising a genetic determinant that leads to the plant having a red colouration of the leaves, which genetic determinant is obtainable from a spinach plant comprising said genetic determinant, representative seed of which was deposited with the NCIMB under accession numbers NCIMB 41954, NCIMB 41955 and NCIMB 41956.

Abstract translation: 本发明涉及菠菜植物（菠菜），其包含导致植物具有红色着色的遗传决定簇，所述遗传决定簇可从包含所述遗传决定簇的菠菜植物获得，其代表性种子被 NCIMB，登录号为NCIMB 41954，NCIMB 41955和NCIMB 41956。

公开(公告)号：WO2014009555A1

公开(公告)日：2014-01-16

申请号：PCT/EP2013/064843

申请日：2013-07-12

Applicant: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

Inventor： BERG, Jurjen Johannes ,  GLAWE, Grit Anja

IPC: A01H5/12 ,  A01H5/10 ,  A01H1/04

CPC classification number: A01H5/08 ,  A01H1/04 ,  A01H5/10 ,  A01H5/12 ,  A23L19/00 ,  A23V2002/00

Abstract: The invention relates to a corn salad fruit of the species Valerianella locusta , characterised by a fruit width which is smaller than 1.7 mm and/or a length/width ratio of the fruits which is higher than 1.2, and which fruit width and/or length/width ratio are/is determined by a genetic determinant which is as found in, or is obtainable from, fruits of which a representative sample was deposited under accession number NCIMB 42007, or is equivalent to the said genetic determinant in NCIMB 42007.

Abstract translation: 本发明涉及一种缬草属（Valerianella locusta）的玉米沙拉水果，其特征在于果实宽度小于1.7mm，和/或水果长度/宽度比高于1.2，果实宽度和/或长度 /宽度比由遗传决定因素确定，该遗传决定簇在代表性样品以保藏号NCIMB 42007存放或与NCIMB 42007中的所述遗传决定簇相当的果实中发现或可获得。