公开(公告)号：WO0065092A3

公开(公告)日：2001-07-19

申请号：PCT/US0010873

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  G01N1/312 ,  C12Q2527/125

Abstract: This invention provides novel methods for detecting microorganisms is biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods involve blocking binding of nucleic acid probe(s) to granulocytes. The method typically involves contacting a biological sample with a nucleic acid probe that specifically hybridizes under stringent conditions to a nucleic acid of a microorganism and a blocking reagent that is a non-coding nucleic acid, washing the biological sample to remove probe that is not hybridized to a nucleic acid of the microorganism, and detecting the probe where the presence of remaining probe indicates the presence of the microorganism.

Abstract translation: 本发明提供了检测微生物的新方法是生物样品。 该方法利用原位杂交技术。 在一个优选的实施方案中，所述方法包括阻断核酸探针与粒细胞的结合。 该方法通常包括使生物样品与在严格条件下与微生物核酸和作为非编码核酸的封闭试剂特异性杂交的核酸探针接触，洗涤生物样品以除去未杂交的探针 涉及微生物的核酸，并检测其中存在的探针表明存在微生物的探针。

公开(公告)号：WO0065092A9

公开(公告)日：2001-09-20

申请号：PCT/US0010873

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  G01N1/312 ,  C12Q2527/125

Abstract: This invention provides novel methods for detecting microorganisms is biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods involve blocking binding of nucleic acid probe(s) to granulocytes. The method typically involves contacting a biological sample with a nucleic acid probe that specifically hybridizes under stringent conditions to a nucleic acid of a microorganism and a blocking reagent that is a non-coding nucleic acid, washing the biological sample to remove probe that is not hybridized to a nucleic acid of the microorganism, and detecting the probe where the presence of remaining probe indicates the presence of the microorganism.

Abstract translation: 本发明提供了检测微生物的新方法是生物样品。 这些方法利用原位杂交技术。 在一个优选的实施方案中，所述方法涉及阻断核酸探针与粒细胞的结合。 该方法通常包括使生物样品与在严格条件下与微生物的核酸和作为非编码核酸的封闭剂特异性杂交的核酸探针接触，洗涤生物样品以除去未杂交的探针 与微生物的核酸结合，并在剩余探针的存在表明存在微生物的情况下检测探针。