公开(公告)号：WO0065092A9

公开(公告)日：2001-09-20

申请号：PCT/US0010873

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  G01N1/312 ,  C12Q2527/125

Abstract: This invention provides novel methods for detecting microorganisms is biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods involve blocking binding of nucleic acid probe(s) to granulocytes. The method typically involves contacting a biological sample with a nucleic acid probe that specifically hybridizes under stringent conditions to a nucleic acid of a microorganism and a blocking reagent that is a non-coding nucleic acid, washing the biological sample to remove probe that is not hybridized to a nucleic acid of the microorganism, and detecting the probe where the presence of remaining probe indicates the presence of the microorganism.

Abstract translation: 本发明提供了检测微生物的新方法是生物样品。 这些方法利用原位杂交技术。 在一个优选的实施方案中，所述方法涉及阻断核酸探针与粒细胞的结合。 该方法通常包括使生物样品与在严格条件下与微生物的核酸和作为非编码核酸的封闭剂特异性杂交的核酸探针接触，洗涤生物样品以除去未杂交的探针 与微生物的核酸结合，并在剩余探针的存在表明存在微生物的情况下检测探针。

公开(公告)号：WO0065093A9

公开(公告)日：2001-08-16

申请号：PCT/US0010874

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6841 ,  G01N1/312

Abstract: This invention provides novel methods for detecting microorganisms in biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods performing an in situ hybridization using two or more probes specific to the same target microorganism where the probes are each labeled with different and distinguishable label or are themselves different and distinguishable. When the probes co-localize to the same feature they are scored as positive for the target microorganism corresponding to those eprobes. Where only a single probe localizes to the target the result is scored as negative and/or, optionally subject to re-testing or other further analysis.

Abstract translation: 本发明提供了检测生物样品中微生物的新方法。 这些方法利用原位杂交技术。 在一个优选的实施方案中，所述方法使用两种或更多种特异于相同靶微生物的探针进行原位杂交，其中探针各自用不同且可区分的标记标记，或者它们本身不同且可区分。 当探针共同定位于相同的特征时，它们被评分为对应于那些探针的靶微生物为阳性。 当只有一个探针定位到目标时，结果评分为阴性和/或可选地受到重新测试或其他进一步分析的影响。

公开(公告)号：WO0065092A3

公开(公告)日：2001-07-19

申请号：PCT/US0010873

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A ,  ALBERTSON DONNA G ,  SEGRAVES RICK

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  G01N1/312 ,  C12Q2527/125

Abstract: This invention provides novel methods for detecting microorganisms is biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods involve blocking binding of nucleic acid probe(s) to granulocytes. The method typically involves contacting a biological sample with a nucleic acid probe that specifically hybridizes under stringent conditions to a nucleic acid of a microorganism and a blocking reagent that is a non-coding nucleic acid, washing the biological sample to remove probe that is not hybridized to a nucleic acid of the microorganism, and detecting the probe where the presence of remaining probe indicates the presence of the microorganism.

Abstract translation: 本发明提供了检测微生物的新方法是生物样品。 该方法利用原位杂交技术。 在一个优选的实施方案中，所述方法包括阻断核酸探针与粒细胞的结合。 该方法通常包括使生物样品与在严格条件下与微生物核酸和作为非编码核酸的封闭试剂特异性杂交的核酸探针接触，洗涤生物样品以除去未杂交的探针 涉及微生物的核酸，并检测其中存在的探针表明存在微生物的探针。

公开(公告)号：WO0065093A2

公开(公告)日：2000-11-02

申请号：PCT/US0010874

申请日：2000-04-21

Applicant: SCIENCE & TECHNOLOGY CORP ,  UNIV NEW MEXICO ,  THOMPSON CURTIS T ,  SPIDLE JOSEPH A

Inventor： THOMPSON CURTIS T ,  SPIDLE JOSEPH A

IPC: C12Q1/68 ,  G01N1/31 ,  B01L7/00

CPC classification number: C12Q1/689 ,  C12Q1/6841 ,  G01N1/312

Abstract: This invention provides novel methods for detecting microorganisms in biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods performing an in situ hybridization using two or more probes specific to the same target microorganism where the probes are each labeled with different and distinguishable label or are themselves different and distinguishable. When the probes co-localize to the same feature they are scored as positive for the target microorganism corresponding to those eprobes. Where only a single probe localizes to the target the result is scored as negative and/or, optionally subject to re-testing or other further analysis.

Abstract translation: 本发明提供用于检测生物样品中微生物的新方法。 该方法利用原位杂交技术。 在一个优选实施方案中，使用两种或更多种对同一靶微生物特异性的探针进行原位杂交的方法，其中探针各自用不同和可区分的标记物标记，或者本身不同且可区分。 当探针共定位到相同的特征时，它们对于与这些eprobes相对应的靶微生物被评分为阳性。 当只有一个探针定位到目标时，结果被评分为阴性和/或可选择进行重新测试或其他进一步的分析。