公开(公告)号：WO2012010871A3

公开(公告)日：2012-03-15

申请号：PCT/GB2011051335

申请日：2011-07-15

Applicant: UNIV EXETER ,  SHAW ANDREW MARK

Inventor： SHAW ANDREW MARK

IPC: G06F19/00

CPC classification number: G01N33/54373 ,  G01N33/564 ,  G01N2333/4716 ,  G01N2333/4724 ,  G01N2800/52 ,  G06F19/18 ,  G06F19/34 ,  G16H50/50

Abstract: We describe a system/method for predicting the outcome of a medical procedure on a patient. The system/method uses using complement cascade data representing levels of a set of complement cascade markers in the patient at a succession of peri-operative time intervals, determining deviations from a model of the response to provide a pre-symptomatic prediction of the outcome. In embodiments the complement cascade pathways include the lytic pathway and at least one of the lectin pathway, the classical pathway and the alternative pathway, and the biomarkers include at least C3. The system may include an electroluminescence or plasmon-resonance multianalyte detector to analyse a blood sample from the patient.

Abstract translation: 我们描述一种用于预测患者的医疗程序结果的系统/方法。 系统/方法使用补体级联数据来表示患者中连续的围手术期间隔的一组补体级联标记的水平，确定与反应模型的偏差以提供结果的预先症状预测。 在实施方案中，补体级联途径包括溶解途径和凝集素途径，经典途径和替代途径中的至少一种，生物标志物至少包括C3。 该系统可以包括电致发光或等离子体共振多分析仪检测器以分析来自患者的血液样品。

公开(公告)号：WO2006054117A2

公开(公告)日：2006-05-26

申请号：PCT/GB2005/050203

申请日：2005-11-16

Applicant: EVANESCO Ltd ,  SHAW, Andrew Mark

Inventor： SHAW, Andrew Mark

IPC: G01F23/292

CPC classification number: G01F23/292

Abstract: This invention is generally concerned with methods and apparatus for optical monitoring of fluid level and quality, in particular by means of evanescent wave techniques such as cavity ring-down spectroscopy. We describe a fuel monitoring system, comprising: an evanescent wave fuel sensor for monitoring said fuel, the sensor comprising a light source and a transducer for providing an electrical signal in response to light from said light source, and having a light path between said light source and said transducer including an evanescent wave sensing region; and a processor coupled to said fuel sensor, for converting said electrical signal from said transducer into a signal characterising said monitored fuel; and an output coupled to said processor, for outputting a signal responsive to said fuel characterising signal. We further describe apparatus for jointly determining depth of fluid in a tank and characterising the fluid according to depth, and a fluid storage tank having a three dimensional structure for containing fluid, and a three-dimensional array of fibre-optic sensors integrated into said tank structure.

Abstract translation: 本发明通常涉及用于液体水平和质量的光学监测的方法和装置，特别是通过诸如空腔衰减光谱的ev逝波技术。 我们描述一种燃料监测系统，包括：用于监测所述燃料的ev逝波燃料传感器，所述传感器包括光源和用于响应于来自所述光源的光提供电信号的换能器，并且在所述光 源和所述换能器包括ev逝波感测区域; 以及耦合到所述燃料传感器的处理器，用于将来自所述换能器的所述电信号转换成表征所述监测燃料的信号; 以及耦合到所述处理器的输出，用于响应于所述燃料特性信号输出信号。 我们进一步描述了用于共同确定罐中流体深度和根据深度表征流体的装置，以及具有用于容纳流体的三维结构的流体储存罐和集成到所述罐中的光纤传感器的三维阵列 结构体。

公开(公告)号：WO2004068123A1

公开(公告)日：2004-08-12

申请号：PCT/GB2004/000020

申请日：2004-01-08

Applicant: SHAW, Andrew, Mark

Inventor： SHAW, Andrew, Mark

IPC: G01N21/55

CPC classification number: G01N21/39 ,  G01J3/42 ,  G01J2003/423

Abstract: This invention is generally concerned with sensing apparatus and methods, more particularly apparatus and methods for sensing techniques based upon cavity ring-down spectroscopy (CRDS). An evanescent wave cavity-based optical sensor is described. The sensor comprises an optical cavity formed by a pair of highly reflective surfaces (108, 110) such that light within said cavity makes a plurality of passes between said surfaces, an optical path between said surfaces including a reflection from a totally internally reflecting (112) surface, said reflection from said reflection from said surface generating an evanescent wave to providing a sensing function; a light source (102) to inject light into said cavity; and a detector (114) to detect a light level within said cavity; whereby absorption of said evanescent wave is detectable using said detector to provide said sensing function; wherein said light source comprises a continuous wave light source; and wherein said light source has a power and bandwidth sufficient to couple energy into at least two modes of oscillation of said cavity to overcome losses within the cavity and excite at least two modes of oscillation of said cavity.

Abstract translation: 本发明一般涉及感测装置和方法，更具体地涉及用于基于空腔衰减光谱（CRDS）的感测技术的装置和方法。 描述了一种消逝波腔基光学传感器。 传感器包括由一对高反射表面（108,110）形成的光学腔，使得所述空腔内的光在所述表面之间形成多个通道，所述表面之间的光路包括来自全内反射的反射（112 ）表面，来自所述表面的所述反射的所述反射产生ev逝波，以提供感测功能; 用于将光注入所述空腔的光源（102） 以及检测器（114），用于检测所述空腔内的光级; 由此使用所述检测器检测所述ev逝波的吸收以提供所述感测功能; 其中所述光源包括连续波光源; 并且其中所述光源具有足以将能量耦合到所述空腔的至少两个振荡模式中的功率和带宽，以克服腔内的损耗并激发所述空腔的至少两种振荡模式。

公开(公告)号：WO2008117087A2

公开(公告)日：2008-10-02

申请号：PCT/GB2008/050202

申请日：2008-03-19

Applicant: UNIVERSITY OF EXETER ,  SHAW, Andrew, Mark ,  OLKHOV, Rouslan, Vladimir ,  BARNES, William, Leslie

Inventor： SHAW, Andrew, Mark ,  OLKHOV, Rouslan, Vladimir ,  BARNES, William, Leslie

IPC: G01N21/21 ,  G01N21/55 ,  G01N33/551 ,  G01N33/553

CPC classification number: G01N21/554 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/211 ,  G01N21/253 ,  G01N33/54313 ,  G01N33/54373 ,  G01N33/553 ,  Y10T436/25

Abstract: This invention relates to photonic biosensor arrays in particular employing plasmon resonance based sensing, and to methods and apparatus for reading such arrays. A biosensor array for plasmon resonance-based sensing of a plurality of different biological targets simultaneously, the array comprising a transparent substrate having a surface bearing a plurality of assay spots for plasmon resonance sensing, each of said assay spots comprising a discrete metallic island, a said metallic island comprising a plurality of metallic nanoparticles to which are attached functionalising molecules for binding to a said biological target, different said islands bearing different said functionalising molecules for binding to different ones of said biological targets, and wherein total internal reflection of light at said surface at a wavelength at or near a said plasmon resonance results in scattering of said light away from said surface, said scattering being modulated by said binding of said biological targets.

Abstract translation: 本发明涉及光子生物传感器阵列，特别是采用基于等离子体共振的感测，以及用于读取这种阵列的方法和装置。 一种生物传感器阵列，用于同时进行基于等离子体共振的多个不同生物靶的感测，所述阵列包括具有表面的透明基底，所述表面具有用于等离子体共振感测的多个测定点，每个所述测定点包括离散的金属岛， 所述金属岛包括多个金属纳米颗粒，其附着有用于结合所述生物靶的功能化分子，不同的所述岛具有不同的所述功能化分子，用于结合不同的所述生物靶，并且其中所述的所述生物靶的全内反射 在所述等离子体共振或其附近的波长处的表面导致所述光离开所述表面的散射，所述散射被所述生物靶的所述结合调制。

公开(公告)号：WO2005088278A1

公开(公告)日：2005-09-22

申请号：PCT/GB2005/050034

申请日：2005-03-15

Applicant: EVANESCO LTD ,  SHAW, Andrew Mark

Inventor： SHAW, Andrew Mark

IPC: G01N21/55

CPC classification number: G01N21/552 ,  G01N2021/7783

Abstract: This invention is generally concerned with sensing techniques based upon evanescent-wave cavity ring-down spectroscopy (CRDS), with particular reference to functionalising a sensing surface with the aim of increasing specificity and/or sensitivity. An evanescent wave cavity-based optical sensor is described. The sensor comprises an optical cavity formed by a pair of highly reflective surfaces such that light within said cavity makes a plurality of passes between said surfaces, an optical path between said surfaces including a reflection from a totally internally reflecting (TIR) surface, said reflection from said TIR surface generating an evanescent wave to provide a sensing function; a light source to inject light into said cavity; and a detector to detect a light level within said cavity ; and wherein said TIR surface is provided with a functionalising material over at least part of said TIR surface such that said evanescent wave interacts with said material ; whereby an interaction between said funtionalising material and a target to be sensed is detectable as a change in absorption of said evanescent wave. Preferably the TIR surface has substantially no features with a dimension perpendicular to the surface of greater than 3µm, more preferably 1µm.

Abstract translation: 本发明通常涉及基于ev逝波腔衰减光谱（CRDS）的感测技术，特别参考功能化感测表面，目的是提高特异性和/或灵敏度。 描述了一种基于ev逝波腔的光学传感器。 传感器包括由一对高反射表面形成的光学腔，使得所述空腔内的光在所述表面之间形成多个通路，所述表面之间的光路包括来自全反射（TIR）表面的反射，所述反射 从所述TIR表面产生ev逝波以提供感测功能; 用于将光注入所述空腔的光源; 以及检测器，用于检测所述空腔内的光级; 并且其中所述TIR表面在所述TIR表面的至少一部分上设置有功能化材料，使得所述ev逝波与所述材料相互作用; 由此所述功能化材料和待感测的靶之间的相互作用可被检测为所述ev逝波的吸收变化。 优选地，TIR表面基本上没有垂直于表面的尺寸大于3μm，更优选1μm的特征。

公开(公告)号：WO2005088274A1

公开(公告)日：2005-09-22

申请号：PCT/GB2005/050036

申请日：2005-03-15

Applicant: EVANESCO Ltd ,  SHAW, Andrew Mark

Inventor： SHAW, Andrew Mark

IPC: G01N21/39

CPC classification number: G01N21/552

Abstract: This invention is generally concerned with apparatus and methods for sensing based upon evanescent-wave cavity ring-down spectroscopy (CRDS), in particular time-resolved and multiplexed sensing techniques. We describe an evanescent wave cavity-based optical sensor, the sensor comprising: an optical cavity formed by a pair of highly reflective surfaces such that light within said cavity makes a plurality of passes between said surfaces, an optical path between said surfaces including a reflection from a totally internally reflecting (TIR) surface, said reflection from said TIR surface generating an evanescent wave to provide a sensing function; a light source to inject a pulse of light into said cavity; a detector to detect decaying oscillations of said light pulse within said cavity; and a signal processor coupled to said detector and configured to provide a time-resolved output responsive to a light level within said cavity and having a time-resolution corresponding to a set of said light pulse oscillations, whereby said sensing function operates at substantially said time-resolution. We further describe a system where one or more TIR surfaces are provided with at least two functionalising materials responsive at different wavelengths such that an interaction between a said functionalising material and one or more targets to be sensed is detectable as a change in absorption of a said evanescent wave at a said wavelength.

Abstract translation: 本发明通常涉及用于基于ev逝波腔衰减光谱（CRDS）进行感测的装置和方法，特别是时间分辨和多路传感技术。 我们描述了一种基于ev逝波腔的光学传感器，该传感器包括：由一对高反射表面形成的光学腔，使得所述腔内的光在所述表面之间形成多个通路，所述表面之间的光路包括反射 来自全内反射（TIR）表面，来自所述TIR表面的所述反射产生ev逝波以提供感测功能; 光源，用于将光脉冲注入所述空腔; 检测器，用于检测所述腔内所述光脉冲的衰减振荡; 以及耦合到所述检测器的信号处理器，其被配置为响应于所述腔内的光级提供时间分辨的输出，并且具有对应于所述光脉冲振荡的集合的时间分辨率，由此所述感测功能基本上在所述时间 -解析度。 我们进一步描述了一种系统，其中一个或多个TIR表面设置有至少两个在不同波长下响应的功能化材料，使得所述功能化材料与待感测的一个或多个靶之间的相互作用可被检测为随着所述 在所述波长处的ev逝波。

公开(公告)号：WO2005088270A2

公开(公告)日：2005-09-22

申请号：PCT/GB2005050035

申请日：2005-03-15

Applicant: EVANESCO LTD ,  SHAW ANDREW MARK

Inventor： SHAW ANDREW MARK

IPC: G01N21/55 ,  G01N21/00

CPC classification number: G01N21/552 ,  G01N21/7703

Abstract: This invention is generally concerned with apparatus and methods for monitoring fluids, in particular critical fluids such as lubricants, based upon evanescent wave techniques. A sensor module, in particular for use with attenuated total internal (ATIR) reflection apparatus for optically determining the condition of a fluid, the sensor module comprising: an evanescent wave sensor; a module housing for said sensor; and an optical connector for connecting said sensor to said ATIR apparatus. Preferably the sensor comprises a tapered optical fibre with a high reflectivity mirror at one or both ends. Also attenuated total internal reflection (TIR) apparatus for the sensor.

Abstract translation: 本发明一般涉及用于基于ev逝波技术来监测流体，特别是关键流体如润滑剂的装置和方法。 传感器模块，特别是用于衰减的全内部（ATIR）反射设备，用于光学地确定流体的状态，所述传感器模块包括：ev逝波传感器; 用于所述传感器的模块外壳; 以及用于将所述传感器连接到所述ATIR设备的光学连接器。 优选地，传感器包括在一端或两端具有高反射镜的锥形光纤。 也是传感器的衰减全内反射（TIR）装置。

公开(公告)号：WO2011104497A1

公开(公告)日：2011-09-01

申请号：PCT/GB2011/000223

申请日：2011-02-18

Applicant: UNIVERSITY OF EXETER ,  TITBALL, Richard William ,  SHAW, Andrew Mark ,  GREGORY, Anthony Edward

Inventor： TITBALL, Richard William ,  SHAW, Andrew Mark ,  GREGORY, Anthony Edward

IPC: A61K47/48 ,  A61K39/02 ,  B82Y5/00 ,  A61P31/04

CPC classification number: A61K39/025 ,  A61K47/646 ,  A61K47/6923 ,  A61K2039/60 ,  A61K2039/627

Abstract: A functionalised gold nanoparticle conjugate comprising a gold nanoparticle linked to at least one antigen, in particular the F1 protein of Yersinia pestis , via a thiol- carboxylic acid linker molecule, such as mercaptodecanoic acid. The reactants are coupled together in the presence of a non-ionic detergent to aid stabilisation of the nanoparticle conjugate to enable its purification and further manipulation.

Abstract translation: 一种功能化的金纳米颗粒缀合物，其包含通过硫醇 - 羧酸连接分子例如巯基癸酸与至少一种抗原，特别是鼠疫耶尔森菌的F1蛋白连接的金纳米颗粒。 反应物在非离子洗涤剂的存在下偶联在一起，以有助于纳米颗粒缀合物的稳定化，使其能够进行纯化和进一步的操作。

公开(公告)号：WO2008117087A3

公开(公告)日：2008-11-13

申请号：PCT/GB2008050202

申请日：2008-03-19

Applicant: UNIV EXETER ,  SHAW ANDREW MARK ,  OLKHOV ROUSLAN VLADIMIR ,  BARNES WILLIAM LESLIE

Inventor： SHAW ANDREW MARK ,  OLKHOV ROUSLAN VLADIMIR ,  BARNES WILLIAM LESLIE

IPC: G01N21/21 ,  G01N21/55 ,  G01N33/551 ,  G01N33/553

CPC classification number: G01N21/554 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/211 ,  G01N21/253 ,  G01N33/54313 ,  G01N33/54373 ,  G01N33/553 ,  Y10T436/25

Abstract: This invention relates to photonic biosensor arrays in particular employing plasmon resonance based sensing, and to methods and apparatus for reading such arrays. A biosensor array for plasmon resonance-based sensing of a plurality of different biological targets simultaneously, the array comprising a transparent substrate having a surface bearing a plurality of assay spots for plasmon resonance sensing, each of said assay spots comprising a discrete metallic island, a said metallic island comprising a plurality of metallic nanoparticles to which are attached functionalising molecules for binding to a said biological target, different said islands bearing different said functionalising molecules for binding to different ones of said biological targets, and wherein total internal reflection of light at said surface at a wavelength at or near a said plasmon resonance results in scattering of said light away from said surface, said scattering being modulated by said binding of said biological targets.

Abstract translation: 本发明涉及特别采用基于等离子体共振的感测的光子生物传感器阵列，并且涉及用于读取这种阵列的方法和装置。 1.一种用于同时基于等离子体共振的多个不同生物靶标感测的生物传感器阵列，所述阵列包含透明基底，所述透明基底具有承载用于等离子体共振感测的多个化验点的表面，所述化验点中的每一个均包含离散金属岛， 所述金属岛包含多个金属纳米颗粒，所述金属纳米颗粒附着有官能化分子以结合到所述生物靶上，不同的所述岛带有不同的所述功能化分子以结合不同的所述生物靶，并且其中所述光的全内反射 在所述等离子体激元共振处或附近的波长处的表面导致所述光从所述表面散射，所述散射通过所述生物靶的所述结合来调节。

公开(公告)号：WO2008117086A1

公开(公告)日：2008-10-02

申请号：PCT/GB2008/050200

申请日：2008-03-19

Applicant: UNIVERSITY OF EXETER ,  SHAW, Andrew, Mark ,  OLKHOV, Rouslan, Vladimir

Inventor： SHAW, Andrew, Mark ,  OLKHOV, Rouslan, Vladimir

IPC: G01N21/55

CPC classification number: B82Y30/00 ,  B22F2998/00 ,  B82Y15/00 ,  G01N21/554 ,  G01N33/54313 ,  G01N33/54373 ,  G01N33/553 ,  Y10T436/25 ,  B22F1/0022

Abstract: This invention relates to a method for the fabrication of photonic biosensor arrays and applications of arrays produced by the method in the biomedical field. A method for the fabrication of a biosensor array for plasmon resonance-based sensing of a plurality of different biological targets simultaneously, the method comprising: (i) providing a transparent substrate; ii) providing seed metallic nanoparticles in the form of a colloid; (iii) depositing said colloid as discrete metallic islands on the transparent substrate, each of said metallic islands comprising a plurality of metallic nanoparticles; (iv) washing the substrate in order to remove unadhered material; (v) developing the substrate in a growth solution, which solution comprises a salt of the same metal which is present in the form of discrete metallic islands on the substrate, a reducing agent, a capping agent and optionally a surfactant; (vi) washing the developed substrate; and (vii) functionalising each of said metallic islands with a different functionalising molecule using a common chemical process to attach said different functionalising molecules to said metallic islands.

Abstract translation: 本发明涉及一种用于制造光子生物传感器阵列的方法和通过该方法在生物医学领域中产生的阵列的应用。 一种制造生物传感器阵列的方法，用于同时进行基于等离子体共振的多个不同生物靶的感测，所述方法包括：（i）提供透明基底; ii）提供胶体形式的种子金属纳米颗粒; （iii）将所述胶体作为离散的金属岛沉积在所述透明基底上，每个所述金属岛包含多个金属纳米颗粒; （iv）洗涤基底以除去未粘附的材料; （v）在生长溶液中显影底物，该溶液包含在基底上以离散金属岛形式存在的相同金属的盐，还原剂，封端剂和任选的表面活性剂; （vi）洗涤显影底物; 和（vii）使用通用化学方法用不同的功能化分子对各个所述金属岛进行功能化，以将所述不同的功能化分子附着到所述金属岛上。