公开(公告)号：WO2009084841A2

公开(公告)日：2009-07-09

申请号：PCT/KR2008/007605

申请日：2008-12-23

Applicant: BIOBUD CO., LTD. ,  SOHN, Young-Doug ,  KIM, Bum Joon ,  KIM, Ok Hwan ,  KIM, Kyoung Jun ,  SHIN, Ji Hun ,  HONG, Sung Yu ,  HWANG, Jae Hoon ,  CHUNG, Kwang Hoe

Inventor： SOHN, Young-Doug ,  KIM, Bum Joon ,  KIM, Ok Hwan ,  KIM, Kyoung Jun ,  SHIN, Ji Hun ,  HONG, Sung Yu ,  HWANG, Jae Hoon ,  CHUNG, Kwang Hoe

IPC: C12N9/64

CPC classification number: C12N9/6418 ,  C07K2319/02

Abstract: The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occuring batroxobin.

Abstract translation: 本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的a因子分泌信号序列，以及使用该序列的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型a因子分泌信号肽序列的蛋白质表达系统获得比天然存在的巴曲酶编码序列高约20倍的重组巴曲酶。 此外，使用本发明的顺序制备的重组巴曲酶与天然发生的巴曲酶相比具有显着似乎合理的活性和稳定性。

公开(公告)号：WO2008102932A1

公开(公告)日：2008-08-28

申请号：PCT/KR2007/002512

申请日：2007-05-23

Applicant: BIOBUD CO., LTD. ,  CHUNG, Kwang Hoe ,  PAK, Chwang Siek ,  HONG, Sung Yu ,  KANG, Soo Jung ,  SOHN, Young Doug ,  HWANG, Jae Hoon ,  CHOI, Eun Bok ,  YON, Gyu Hwan ,  LEE, Hyeon-Kyu ,  YANG, Heui Cheol

Inventor： CHUNG, Kwang Hoe ,  PAK, Chwang Siek ,  HONG, Sung Yu ,  KANG, Soo Jung ,  SOHN, Young Doug ,  HWANG, Jae Hoon ,  CHOI, Eun Bok ,  YON, Gyu Hwan ,  LEE, Hyeon-Kyu ,  YANG, Heui Cheol

IPC: A61K31/47

CPC classification number: A61K31/47

Abstract: The present invention relates to a pharmaceutical composition for preventing or treating hyperproliferative vascular disorders, and a pharmaceutical anticancer composition comprising the compound represented by the Formula 1. The present compounds exhibit IC 50 values of less than 0.16 μM for vascular smooth muscle cells and cancer cells to effectively prevent proliferation of vascular smooth muscle cells and cancer cells, thereby ensuring prevention or treatment of hyperproliferative vascular disorders such as arteriosclerosis and restenosis, and cancers.

Abstract translation: 本发明涉及用于预防或治疗过度增殖性血管疾病的药物组合物，以及含有式1表示的化合物的药物抗癌组合物。本发明化合物的IC 50值小于0.16μM， 血管平滑肌细胞和癌细胞，以有效地防止血管平滑肌细胞和癌细胞的增殖，从而确保预防或治疗过度增生性血管疾病如动脉硬化和再狭窄以及癌症。

公开(公告)号：WO2009084841A3

公开(公告)日：2009-09-17

申请号：PCT/KR2008007605

申请日：2008-12-23

Applicant: BIOBUD CO LTD ,  SOHN YOUNG-DOUG ,  KIM BUM JOON ,  KIM OK HWAN ,  KIM KYOUNG JUN ,  SHIN JI HUN ,  HONG SUNG YU ,  HWANG JAE HOON ,  CHUNG KWANG HOE

Inventor： SOHN YOUNG-DOUG ,  KIM BUM JOON ,  KIM OK HWAN ,  KIM KYOUNG JUN ,  SHIN JI HUN ,  HONG SUNG YU ,  HWANG JAE HOON ,  CHUNG KWANG HOE

IPC: C12N9/64

CPC classification number: C12N9/6418 ,  C07K2319/02

Abstract: The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated a-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated a-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occuring batroxobin.

Abstract translation: 本发明涉及编码巴曲酶的核苷酸序列和/或突变的α-因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高4-13倍的产率获得重组巴曲酶。 使用本发明的巴曲酶编码核苷酸序列以及突变的α-因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高约20倍的产量获得重组巴曲酶。 此外，与天然存在的巴曲酶相比，使用本发明的序列制备的重组巴曲酶具有显着合理的活性和稳定性。

公开(公告)号：WO2003018065A1

公开(公告)日：2003-03-06

申请号：PCT/KR2001/001461

申请日：2001-08-29

Applicant: CHUNG, Kwang-Hoe ,  PARK, Yong-Serk ,  KIM, Soo-In ,  HONG, Sung-Yu ,  SOHN, Young-Doug ,  JANG, Yang-Soo ,  HUN, Chin-Kyu ,  KIM, Doo-Sik

Inventor： CHUNG, Kwang-Hoe ,  PARK, Yong-Serk ,  KIM, Soo-In ,  HONG, Sung-Yu ,  SOHN, Young-Doug ,  JANG, Yang-Soo ,  HUN, Chin-Kyu ,  KIM, Doo-Sik

IPC: A61K48/00

CPC classification number: C12N15/88 ,  A61K9/127 ,  A61K48/00 ,  A61K48/0025 ,  C07K14/46 ,  C12N15/86 ,  C12N2750/14143

Abstract: The present invention relates to liposome complex comprising a novel disintegrin, saxatilin, gene derived from Agkistrodon saxatilis and methods for curing and preventing tumors by transferring the complexes to a living body.

Abstract translation: 本发明涉及脂质体复合物，其包含新颖的解联蛋白，萨克西汀，源自Ag蛇毒素的基因，以及通过将复合物转移到活体来固化和预防肿瘤的方法。