公开(公告)号：WO2006090241A2

公开(公告)日：2006-08-31

申请号：PCT/IB2006/000360

申请日：2006-02-22

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIVERSITY OF CAPE TOWN ,  BOURN, William ,  WILLIAMSON, Anna-lise

发明人： BOURN, William ,  WILLIAMSON, Anna-lise

IPC分类号： C12N15/67 ,  C12N15/09 ,  C12N15/62 ,  C07K19/00 ,  C07K16/08

CPC分类号： C12N15/1058

摘要： The invention describes a method for identifying modified versions of a foreign recombinant peptide, polypeptide or protein, expressed from a cloned gene, which are produced and maintained in a host cell at high levels and which are of reduced toxicity, such that genetic stability of the recombinant DNA is increased. This is achieved by a dual selection process whereby the modified protein is tested both (i) for its ability to accumulate and (ii) for its ability to cause reduced host stress.

摘要翻译： 本发明描述了用于鉴定由克隆基因表达的外源重组肽，多肽或蛋白质的修饰形式的方法，所述外源重组肽，多肽或蛋白质以高水平产生并保持在宿主细胞中，并且其被还原 毒性，使得重组DNA的遗传稳定性增加。 这是通过双重选择过程实现的，其中修饰的蛋白质既被测试（i）其积累能力和（ii）其引起宿主应激减少的能力。

公开(公告)号：WO2006048734A1

公开(公告)日：2006-05-11

申请号：PCT/IB2005/003268

申请日：2005-11-02

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIVERSITY OF CAPE TOWN ,  MATSABISA, Motlalepula, Gilbert ,  CAMPBELL, William, Ernest ,  FOLB, Peter, Ian ,  SMITH, Peter, John

发明人： MATSABISA, Motlalepula, Gilbert ,  CAMPBELL, William, Ernest ,  FOLB, Peter, Ian ,  SMITH, Peter, John

IPC分类号： A61K36/28 ,  A61P33/06

CPC分类号： A61K36/28 ,  Y02A50/411

摘要： The invention provides a process for the production of a substance or composition for the treatment, by therapy or prophylaxis, of parasitic infections, in particular malarial infections such as Plasmodium falciparum infections, of the human or animal body. The process comprises extracting the substance or composition from roots of the plant species Dicoma anomala, by an extraction using an organic solvent to obtain a liquid extract containing the substance or composition and removing the solvent from the liquid extract to leave a dried extract containing the substance or composition. The invention extends also to the use of the substance or composition in the manufacture of a medicament or preparation for such treatment of infections; to a substance or composition for use in such treatment of said infections; to compounds for use in such treatment of said infections; and to a method of treating said infections using such compounds.

摘要翻译： 本发明提供了用于通过治疗或预防人或动物体的寄生虫感染，特别是疟疾恶性疟原虫感染的疟疾感染来生产物质或组合物的方法。 该方法包括从植物物种Dicoma异常的根中提取物质或组合物，通过使用有机溶剂的萃取获得含有该物质或组合物的液体提取物，并从液体提取物中除去溶剂以留下含有该物质的干燥提取物 或组成。 本发明还涉及物质或组合物在制备用于感染的这种治疗的药物或制剂中的用途; 涉及用于治疗所述感染的物质或组合物; 用于治疗所述感染的化合物; 以及使用这些化合物治疗所述感染的方法。

公开(公告)号：WO2004050691A3

公开(公告)日：2004-06-17

申请号：PCT/IB2003/005634

申请日：2003-12-04

申请人： UNIVERSITY OF CAPE TOWN ,  SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  JAFFRAY, Ann ,  WILLIAMSON, Anna-Lise ,  RYBICKI, Edward, Peter

发明人： JAFFRAY, Ann ,  WILLIAMSON, Anna-Lise ,  RYBICKI, Edward, Peter

IPC分类号： C07K14/16

摘要： The invention describes a vector including a nucleotide sequence encoding an HIV Gag polypeptide for use in the production of HIV-1 Gag virus-like particles. The vector may be a plant vector, for example, a tobacco mosaic virus vector such as the pBSG1057 vector or a tobacco etch virus vector. The vector may also be an Agrobacterium tumefaciens containing a T-derived plasmid construct. Alternatively, the vector may be an insect vector such as a baculovirus vector. HIV-1 Gag virus-like particles are also described, as is the use of the virus-like particles in a vaccine for use in the treatment or prophylaxis of HIV infection in a mammal, the vaccine including virus-like particles of proteins or polypeptides substantially as described above.

公开(公告)号：WO2023042070A1

公开(公告)日：2023-03-23

申请号：PCT/IB2022/058615

申请日：2022-09-13

申请人： STELLENBOSCH UNIVERSITY ,  SOUTH AFRICAN MEDICAL RESEARCH COUNCIL

发明人： DIJKSTRA, Stephan ,  THERON, Grant De Vos ,  NIEUWOUDT, Martinus Johannes ,  VENTER, Rouxjeane ,  WARREN, Robin Mark

IPC分类号： B01L3/00 ,  B01L9/06 ,  B01L9/00

摘要： An extraction device assembly for extracting a sample from a sample-containing chamber of a nucleic acid amplification cartridge is provided. The assembly has a cartridge interface forming a receptacle configured to at least partially fit the sample-containing chamber therein. The cartridge interface has an aperture configured to at least partially expose the sample-containing chamber, and a collection interface having an aperture configured to at least partially align with the cartridge interface aperture and with the sample-containing chamber positioned at least partially between them. The collection interface aperture is in fluid communication with a collector. The extraction device assembly is operable to fit the sample-containing chamber in the receptacle of the cartridge interface and guide a piercing tool into the cartridge interface aperture to pierce the sample-containing chamber and liberate a sample contained therein for transferral to the collector.

公开(公告)号：WO2011064726A1

公开(公告)日：2011-06-03

申请号：PCT/IB2010/055398

申请日：2010-11-24

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  VAN DER WESTHUIZEN, Jan Hendrik ,  FERREIRA, Daneel ,  JOUBERT, Elizabeth ,  BONNET, Sussana Lucia

发明人： VAN DER WESTHUIZEN, Jan Hendrik ,  FERREIRA, Daneel ,  JOUBERT, Elizabeth ,  BONNET, Sussana Lucia

IPC分类号： C07H3/02 ,  C07H1/00

CPC分类号： C07H15/203

摘要： A method of synthesising Aspalathin and its analogues or derivatives is disclosed. The method comprises synthesising a compound of formula 1 or its analogues or derivatives (I) wherein each of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 and R 10 is independently selected from the group consisting of -H, -OH, hydrocarbyl groups, saccharide moieties and -OR 15 ; R 15 is selected from the group consisting of hydrogen, a hydrocarbyl group (e.g. methoxy or ethoxy), an acyl group and a benzyl group; and R 11 , R 12 , R 13 and R 14 are independently selected from the group consisting of -H, hydrocarbyl groups, saccharide moieties, an acyl group and a benzyl group. The method comprises the step of coupling a sugar to a dihydrochalcone, chalcone or flavanone, or coupling the sugar to an intermediate for producing a dihydrochalcone, chalcone or flavanone followed by coupling of the sugar-intermediate adduct to a further intermediate for producing a dihydrochalcone, chalcone or flavanone, and transforming the product thereof into a compound of formula 1 or an analogue or derivative thereof.

摘要翻译： 公开了一种合成Aspalathin及其类似物或衍生物的方法。 该方法包括合成式1化合物或其类似物或衍生物（I），其中R 1，R 2，R 3，R 4，R 5，R 6，R 7，R 8，R 9和R 10各自独立地选自-H， -OH，烃基，糖部分和-OR15; R 15选自氢，烃基（例如甲氧基或乙氧基），酰基和苄基; R 11，R 12，R 13和R 14独立地选自-H，烃基，糖部分，酰基和苄基。 该方法包括将糖偶联到二氢查耳酮，查耳酮或黄酮酮或将糖偶联到用于制备二氢查耳酮，查耳酮或黄烷酮的中间体，然后将糖 - 中间体加合物偶联到另外的中间体以产生二氢查耳酮， 查耳酮或黄烷酮，并将其产物转化为式1的化合物或其类似物或衍生物。

公开(公告)号：WO2011012988A1

公开(公告)日：2011-02-03

申请号：PCT/IB2010/001876

申请日：2010-07-29

申请人： UNIVERSITY OF CAPE TOWN ,  SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  JOHN, Lestor, Ryan

发明人： JOHN, Lestor, Ryan

IPC分类号： A61B5/0488

CPC分类号： A61B5/0492 ,  A61B5/04012 ,  A61B5/0488 ,  A61B5/0536 ,  A61B5/1107 ,  A61B5/4519 ,  A61B5/6802 ,  A61B5/6824 ,  A61B5/6831 ,  A61B5/7203 ,  A61B5/7264 ,  A61B2562/0209 ,  A61B2562/043 ,  G06K9/0057 ,  G06K9/624

摘要： A method and apparatus for conducting electromyography of a deep muscle non-invasively are provided. An array of suitable surface electromyography electrodes (3) is arranged in one or more rings encircling a part of the human body in which a deep muscle (c) being investigated is located. The potential of at least selected electrodes relative to another electrode selected from a common reference electrode (mono-polar) and other electrodes (bi-polar) in the array is recorded and the data is processed in respect of the recorded potentials of at least some of said selected electrodes in order to determine (optionally using approximations or algorithms, or both) the contribution being made by at least the deep muscle being investigated. Typically, this is done mathematically by resolving the electromyography signals into their constituent components using a suitable technique. Preferably, this is integrated with a static muscle imaging device that may use the same electrodes to obtain a static tomogram of the muscles encircled.

摘要翻译： 提供了非侵入式进行肌肉肌电图的方法和装置。 合适的表面肌电图电极（3）的阵列布置在一个或多个环中，环绕被检查的深层肌肉（c）的人体的一部分。 至少选择的电极相对于从阵列中的公共参考电极（单极性）和其他电极（双极性）中选择的另一电极的电位被记录，并且关于至少一些的记录电位处理数据 以确定（可选地使用近似值或算法，或两者​​）由至少所研究的深层肌肉进行的贡献。 通常，这是通过使用合适的技术将肌电描记信号解析成其组成成分在数学上完成的。 优选地，其与静态肌肉成像装置整合，其可以使用相同的电极来获得包围的肌肉的静态断层图像。

公开(公告)号：WO2009101604A3

公开(公告)日：2009-11-12

申请号：PCT/IB2009050624

申请日：2009-02-16

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIV CAPE TOWN ,  WILLIAMSON ANNA-LISE ,  DOUGLASS NICOLA ,  SHEN YEN-JU

发明人： WILLIAMSON ANNA-LISE ,  DOUGLASS NICOLA ,  SHEN YEN-JU

IPC分类号： A61K39/21

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C12N15/86 ,  C12N2710/24143 ,  C12N2740/16034

摘要： The invention describes a method of manufacturing a recombinant nucleic acid molecule and virus for delivering one or more HIV antigens to a subject, the method including the step of inserting one or more human immunodeficiency virus (HIV) sequences into a lumpy skin disease (LSD) virus nucleotide sequence or virus. The HIV sequences are capable of being expressed from the LSD virus sequence as one or more HIV proteins or immunogenic parts thereof. Recombinant LSD nucleic acid molecules and viruses are also described. Pharmaceutical compositions including the recombinant virus may be used for the inhibition of AIDS in a human.

摘要翻译： 本发明描述了制备用于将一种或多种HIV抗原递送至受试者的重组核酸分子和病毒的方法，所述方法包括将一种或多种人类免疫缺陷病毒（HIV）序列插入块状皮肤病（LSD） 病毒核苷酸序列或病毒。 HIV序列能够作为一种或多种HIV蛋白质或其免疫原性部分从LSD病毒序列表达。 还描述了重组LSD核酸分子和病毒。 包含重组病毒的药物组合物可用于抑制人类中的AIDS。

公开(公告)号：WO2008001306A2

公开(公告)日：2008-01-03

申请号：PCT/IB2007/052468

申请日：2007-06-26

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIVERSITY OF CAPE TOWN ,  MEYERS, Paul Robert ,  LE ROES, Marilize ,  GAMMON, David W

发明人： MEYERS, Paul Robert ,  LE ROES, Marilize ,  GAMMON, David W

IPC分类号： C12R1/465

CPC分类号： C12R1/465 ,  C12P17/00

摘要： The present invention relates to an isolated microorganism of the strain type SPR T (=DSM 44925 T = NRRL B-24448 T ). The microorganism preferably includes genetic material of GenBank accession number DQ141528 (16S-rDNA-sequence). The invention extends to a method of producing 2,5-diphenyloxazole or variants or derivatives thereof, the method comprising recovering the 2,5-diphenyloxazole or variants or derivatives thereof from a microorganism of the strain typeSPR T (=DSM44925 T = NRRL B-24448 T ).

摘要翻译： 本发明涉及SPR型菌株（= DSM 44925 T = NRRL B-24448 T）的分离的微生物。 微生物优选包括GenBank登录号DQ141528（16S-rDNA序列）的遗传物质。 本发明涉及生产2,5-二苯基恶唑或其变体或衍生物的方法，该方法包括从菌株类型SPR 的微生物中回收2,5-二苯基恶唑或其变体或衍生物 = DSM44925 = NRRL B-24448 ）。

公开(公告)号：WO2009101604A2

公开(公告)日：2009-08-20

申请号：PCT/IB2009/050624

申请日：2009-02-16

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIVERSITY OF CAPE TOWN ,  WILLIAMSON, Anna-Lise ,  DOUGLASS, Nicola ,  SHEN, Yen-Ju

发明人： WILLIAMSON, Anna-Lise ,  DOUGLASS, Nicola ,  SHEN, Yen-Ju

IPC分类号： A61K39/21

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C12N15/86 ,  C12N2710/24143 ,  C12N2740/16034

摘要： The invention describes a method of manufacturing a recombinant nucleic acid molecule and virus for delivering one or more HIV antigens to a subject, the method including the step of inserting one or more human immunodeficiency virus (HIV) sequences into a lumpy skin disease (LSD) virus nucleotide sequence or virus. The HIV sequences are capable of being expressed from the LSD virus sequence as one or more HIV proteins or immunogenic parts thereof. Recombinant LSD nucleic acid molecules and viruses are also described. Pharmaceutical compositions including the recombinant virus may be used for the inhibition of AIDS in a human.

摘要翻译： 本发明描述了制备用于将一种或多种HIV抗原递送至受试者的重组核酸分子和病毒的方法，所述方法包括插入一种或多种人类免疫缺陷病毒（HIV）序列 成为块状皮肤病（LSD）病毒核苷酸序列或病毒。 HIV序列能够从LSD病毒序列表达为一种或多种HIV蛋白或其免疫原性部分。 还描述了重组LSD核酸分子和病毒。 包含重组病毒的药物组合物可用于抑制人类中的AIDS。

公开(公告)号：WO2007054792A1

公开(公告)日：2007-05-18

申请号：PCT/IB2006/003155

申请日：2006-11-08

申请人： SOUTH AFRICAN MEDICAL RESEARCH COUNCIL ,  UNIVERSITY OF CAPE TOWN ,  HALSEY, Richard, James ,  TANZER, Fiona, Lesley ,  WILLIAMSON, Anna-Lise ,  RYBICKI, Edward, Peter

发明人： HALSEY, Richard, James ,  TANZER, Fiona, Lesley ,  WILLIAMSON, Anna-Lise ,  RYBICKI, Edward, Peter

IPC分类号： C07K14/16

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  C07K14/005 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2750/14143

摘要： A method of producing immunogenic chimaeric proteins which self-assemble into virus-like particles is described. A first DNA sequence encoding a HIV-1 subtype C protein or portion thereof of greater than 200 amino acids is fused to a second DNA sequence encoding at least p17 and p24 of a Gag protein by a direct in-frame C-terminal fusion. A host cell is infected with a vector containing the fused sequence, and the chimaeric protein encoded by the fused sequence is expressed. The second DNA sequence may aalternatively be a full-length gag sequence or a truncated gag sequence. Examples of the first DNA sequence are provided for tat, nef and reverse transcriptase, and combinations thereof.

摘要翻译： 描述了自组装成病毒样颗粒的产生免疫原性嵌合蛋白的方法。 将编码HIV-1亚型C蛋白或其大于200个氨基酸的部分的第一DNA序列通过直接的框内C末端融合融合到编码Gag蛋白质的至少p17和p24的第二DNA序列。 用含有融合序列的载体感染宿主细胞，并表达由融合序列编码的嵌合蛋白。 第二DNA序列可以替代地是全长gag序列或截短的gag序列。 第一种DNA序列的实例用于tat，nef和reverse transcriptase及其组合。