公开(公告)号：WO2006066180A1

公开(公告)日：2006-06-22

申请号：PCT/US2005/045874

申请日：2005-12-15

Applicant: INTEL CORPORATION ,  SUN, Lei ,  SU, Xing

Inventor： SUN, Lei ,  SU, Xing

IPC: G01N33/543

CPC classification number: G01N21/658 ,  G01N33/54306

Abstract: Method comprising a) a target analyte associated with a first specific binding member; b) a second specific binding member that binds to the first specific bonding member forming a target complex, wherein the second specific binding member comprises s seed particle suitable for catalyzing the formation of a surface enhanced Raman scattering (SERS) substrate, wherein the SERS substrate can be activated to provide a SERS effect; and c) capture reagent bound to a solid substrate, wherein the capture reagent comprises a Raman label, wherein the target analyte binds to the capture reagent forming a target complex.

Abstract translation: 方法包括a）与第一特异性结合成员相关联的目标分析物; b）与形成目标配合物的第一特异性结合构件结合的第二特异性结合构件，其中第二特异性结合构件包含适于催化形成表面增强拉曼散射（SERS）底物的s种子颗粒，其中SERS底物 可以激活提供SERS效应; 和c）捕获与固体底物结合的试剂，其中所述捕获试剂包含拉曼标记，其中所述靶分析物与形成目标复合物的捕获试剂结合。

公开(公告)号：WO2005065541A3

公开(公告)日：2005-07-21

申请号：PCT/US2004/043769

申请日：2004-12-29

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo

IPC: G01N33/58

Abstract: The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

公开(公告)号：WO2023082231A1

公开(公告)日：2023-05-19

申请号：PCT/CN2021/130536

申请日：2021-11-15

Applicant: MICROSOFT TECHNOLOGY LICENSING, LLC ,  LI, Haoxuan ,  JIANG, Rui ,  LIU, Yang ,  LIN, Edward C ,  SUN, Lei ,  ZHAO, Che

Inventor： LI, Haoxuan ,  JIANG, Rui ,  LIU, Yang ,  LIN, Edward C ,  SUN, Lei ,  ZHAO, Che

IPC: G10L15/06 ,  G10L15/183 ,  G10L13/00

Abstract: Systems and methods are provided for identifying targeted datasets that are configured to facilitate an improvement in the accuracy of an acoustic model included in the automatic speech recognition system. Systems obtain a obtain a test dataset comprising (i) audio data having natural speech utterances and (ii) a transcription of the natural speech utterances. Systems generate a text-to-speech dataset comprising audio data having synthesized speech utterances based on the transcription of the natural speech utterances. Systems apply the test dataset and the text-to-speech dataset to the acoustic model to obtain a first acoustic model output and a second acoustic model output, respectively. Systems identify a first set of errors in the first acoustic model output and a second set of errors in the second acoustic model output. Finally, based on comparing the first set of errors and the second set of errors, an acoustic model error ratio is generated.

公开(公告)号：WO2018072056A1

公开(公告)日：2018-04-26

申请号：PCT/CN2016/102250

申请日：2016-10-17

Applicant: MICROSOFT TECHNOLOGY LICENSING, LLC ,  LU, Yumao ,  LI, Ling ,  SUN, Lei ,  MA, Guangxu ,  ZHU, Yue

Inventor： LU, Yumao ,  LI, Ling ,  SUN, Lei ,  MA, Guangxu ,  ZHU, Yue

IPC: H04L29/06

CPC classification number: H04L65/4084 ,  H04N21/23418 ,  H04N21/2743 ,  H04N21/4788 ,  H04N21/854

Abstract: The present disclosure provides method, apparatus and system for sharing network content. A content sample of a network content to be shared may be obtained. A comment video corresponding to the network content may be obtained. A remixing process may be performed on the content sample and the comment video based on object features of the comment video.

Abstract translation: 本公开提供了用于共享网络内容的方法，设备和系统。 可以获得要共享的网络内容的内容样本。 可以获得与网络内容相对应的评论视频。 可以基于评论视频的对象特征对内容样本和评论视频执行再混合处理。

公开(公告)号：WO2012044987A3

公开(公告)日：2012-10-04

申请号：PCT/US2011054318

申请日：2011-09-30

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV JOHNS HOPKINS ,  UNIV NORTH CAROLINA ,  UNIV CASE WESTERN RESERVE ,  STRUG LISA ,  SUN LEI ,  ROMMENS JOHANNA M ,  DURIE PETER ,  CUTTING GARRY ,  KOWLES MICHAEL ,  DRUMM MITCHELL

Inventor： STRUG LISA ,  SUN LEI ,  ROMMENS JOHANNA M ,  DURIE PETER ,  CUTTING GARRY ,  KOWLES MICHAEL ,  DRUMM MITCHELL

IPC: C12Q1/68 ,  C12N15/12 ,  G01N33/15

CPC classification number: C12Q1/6883 ,  C07K14/4712 ,  C12Q2600/156

Abstract: Disclosed herein are compositions and methods for and treating Cystic Fibrosis lung disease severity and/or secondary manifestations, including meconium ileus and CF related liver disease.

Abstract translation: 本文公开了用于和治疗囊性纤维化肺疾病严重性和/或二级表现的组合物和方法，包括胎粪肠梗阻和CF相关的肝脏疾病。

公开(公告)号：WO2012044987A2

公开(公告)日：2012-04-05

申请号：PCT/US2011/054318

申请日：2011-09-30

Applicant: HOSPITAL FOR SICK CHILDERN ,  THE JOHNS HOPKINS UNIVERSITY ,  UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  CASE WESTERN RESERVE UNIVERSITY ,  STRUG, Lisa ,  SUN, Lei ,  ROMMENS, Johanna, M. ,  CUTTING, Garry ,  KOWLES, Michael ,  DRUMM, Mitchell ,  DURIE, Peter

Inventor： STRUG, Lisa ,  SUN, Lei ,  ROMMENS, Johanna, M. ,  CUTTING, Garry ,  KOWLES, Michael ,  DRUMM, Mitchell ,  DURIE, Peter

IPC: C12Q1/68 ,  C12N15/12 ,  G01N33/15

CPC classification number: C12Q1/6883 ,  C07K14/4712 ,  C12Q2600/156

Abstract: Disclosed herein are compositions and methods for and treating Cystic Fibrosis lung disease severity and/or secondary manifestations, including meconium ileus and CF related liver disease.

Abstract translation: 本文公开了用于并治疗囊性纤维化肺病严重性和/或继发表现（包括胎粪肠梗阻和CF相关性肝病）的组合物和方法。

公开(公告)号：WO2008005899A2

公开(公告)日：2008-01-10

申请号：PCT/US2007072595

申请日：2007-06-29

Applicant: INTEL CORP ,  SUN LEI ,  KOO TAE WOONG ,  WANG LIMING

Inventor： SUN LEI ,  KOO TAE WOONG ,  WANG LIMING

CPC classification number: G01N21/658 ,  G01J3/36 ,  G01J3/44

Abstract: A device (and methods of using and manufacturing the device) that utilize a plurality of photomultipliers (PMT)s or a photodiodes coupled with a set of filters to collect Raman signal from samples. Also a method of detecting Raman signals includes receiving Raman signals from a sample utilizing a plurality of photomultiplier tubes (PMT)s or photodiodes, wherein at least one PMT or photodiode provides a different Raman signal than at least one other PMT or photodiode.

Abstract translation: 使用多个光电倍增管（PMT）或与一组滤波器耦合的光电二极管以从样品收集拉曼信号的装置（以及使用和制造该装置的方法）。 检测拉曼信号的方法还包括利用多个光电倍增管（PMT）或光电二极管从样品接收拉曼信号，其中至少一个PMT或光电二极管提供与至少一个其它PMT或光电二极管不同的拉曼信号。

公开(公告)号：WO2006023458A9

公开(公告)日：2006-04-06

申请号：PCT/US2005029019

申请日：2005-08-13

Applicant: INTEL CORP ,  SUNDARARAJAN NARAYAN ,  SUN LEI ,  SU XING ,  YAMAKAWA MINEO ,  JINGWU ZHANG ,  CHAN SELENA ,  BERLIN ANDREW ,  KOO TAE-WOONG ,  ROTH MARK ,  GAFEN PHIL

Inventor： SUNDARARAJAN NARAYAN ,  SUN LEI ,  SU XING ,  YAMAKAWA MINEO ,  JINGWU ZHANG ,  CHAN SELENA ,  BERLIN ANDREW ,  KOO TAE-WOONG ,  ROTH MARK ,  GAFEN PHIL

IPC: G01N33/68 ,  G01N21/65

CPC classification number: G01N33/6851 ,  G01N21/658 ,  G01N33/54373 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  Y02A90/26

Abstract: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diognosis, prognosis and drug efficacy studies, emzymatic activity profiling and assays.

Abstract translation: 本发明的实施方案提供了使用表面增强拉曼（SERS）和拉曼光谱法检测，鉴定，区分和定量蛋白质和肽的修饰状态的装置和方法。 本发明的实施方案的应用包括例如蛋白质组广泛的修饰分析和分析，其应用于疾病预后，预后和药物功效研究，酶活性分析和测定。

公开(公告)号：WO2005066369A3

公开(公告)日：2006-02-02

申请号：PCT/US2004043633

申请日：2004-12-28

Applicant: INTEL CORP ,  BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN

Inventor： BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN

IPC: C12Q1/68 ,  G01N21/65

CPC classification number: G01N21/658 ,  C12Q1/6869 ,  C12Q2565/632 ,  C12Q2533/101

Abstract: The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

Abstract translation: 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸掺入的顺序来确定。 提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。 此外，提供了用于检测单碱基重复的方法。

公开(公告)号：WO2005066369A2

公开(公告)日：2005-07-21

申请号：PCT/US2004/043633

申请日：2004-12-28

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

IPC: C12Q1/68

CPC classification number: G01N21/658 ,  C12Q1/6869 ,  C12Q2565/632 ,  C12Q2533/101

Abstract: The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

Abstract translation: 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸掺入的顺序来确定。 提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。 此外，提供了用于检测单碱基重复的方法。