公开(公告)号：WO2006066180A1

公开(公告)日：2006-06-22

申请号：PCT/US2005/045874

申请日：2005-12-15

Applicant: INTEL CORPORATION ,  SUN, Lei ,  SU, Xing

Inventor： SUN, Lei ,  SU, Xing

IPC: G01N33/543

CPC classification number: G01N21/658 ,  G01N33/54306

Abstract: Method comprising a) a target analyte associated with a first specific binding member; b) a second specific binding member that binds to the first specific bonding member forming a target complex, wherein the second specific binding member comprises s seed particle suitable for catalyzing the formation of a surface enhanced Raman scattering (SERS) substrate, wherein the SERS substrate can be activated to provide a SERS effect; and c) capture reagent bound to a solid substrate, wherein the capture reagent comprises a Raman label, wherein the target analyte binds to the capture reagent forming a target complex.

Abstract translation: 方法包括a）与第一特异性结合成员相关联的目标分析物; b）与形成目标配合物的第一特异性结合构件结合的第二特异性结合构件，其中第二特异性结合构件包含适于催化形成表面增强拉曼散射（SERS）底物的s种子颗粒，其中SERS底物 可以激活提供SERS效应; 和c）捕获与固体底物结合的试剂，其中所述捕获试剂包含拉曼标记，其中所述靶分析物与形成目标复合物的捕获试剂结合。

公开(公告)号：WO2005065541A3

公开(公告)日：2005-07-21

申请号：PCT/US2004/043769

申请日：2004-12-29

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo

IPC: G01N33/58

Abstract: The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

公开(公告)号：WO2006023458A1

公开(公告)日：2006-03-02

申请号：PCT/US2005/029019

申请日：2005-08-13

Applicant: INTEL CORPORATION ,  SUNDARARAJAN, Narayan ,  SUN, Lei ,  SU, Xing ,  YAMAKAWA, Mineo ,  JINGWU, Zhang ,  CHAN, Selena ,  BERLIN, Andrew ,  KOO, Tae-Woong ,  ROTH, Mark ,  GAFEN, Phil

Inventor： SUNDARARAJAN, Narayan ,  SUN, Lei ,  SU, Xing ,  YAMAKAWA, Mineo ,  JINGWU, Zhang ,  CHAN, Selena ,  BERLIN, Andrew ,  KOO, Tae-Woong ,  ROTH, Mark ,  GAFEN, Phil

IPC: G01N33/68 ,  G01N21/65

CPC classification number: G01N33/6851 ,  G01N21/658 ,  G01N33/54373 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  Y02A90/26

Abstract: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diognosis, prognosis and drug efficacy studies, emzymatic activity profiling and assays.

Abstract translation: 本发明的实施例提供了用于使用表面增强拉曼（SERS）和拉曼光谱来检测，鉴定，区分和量化蛋白质和肽的修饰状态的装置和方法。 本发明实施方案的应用包括例如蛋白质组广泛的修饰分析和应用于疾病预后，预后和药物功效研究，酶活性分析和测定的分析。

公开(公告)号：WO2005090948A2

公开(公告)日：2005-09-29

申请号：PCT/US2004/043878

申请日：2004-12-28

Applicant: INTEL CORPORATION ,  SU, Xing ,  ZHANG, Jingwu ,  SUN, Lei ,  BERLIN, Andrew

Inventor： SU, Xing ,  ZHANG, Jingwu ,  SUN, Lei ,  BERLIN, Andrew

IPC: G01N21/65

CPC classification number: G01N33/54346 ,  G01N21/658

Abstract: Composite organic-inorganic nanoclusters (COINs) are provided that produce surface-enhanced Raman signals (SERS) when excited by a laser. The nanoclusters include metal particles and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanocluster and a wide variety of Raman-active organic compounds and combinations thereof can be incorporated into the nanocluster. In addition, polymeric microspheres containing the nanoclusters and methods of making them are also provided. The nanoclusters and microspheres can be used, for example, in assays for multiplex detection of biological molecules.

Abstract translation: 提供了复合有机 - 无机纳米团簇（COINs），当激光激发时产生表面增强拉曼信号（SERS）。 纳米团簇包括金属颗粒和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米簇中是固有的，并且可以将多种拉曼活性有机化合物及其组合掺入纳米团簇中。 此外，还提供了含有纳米簇的聚合物微球及其制备方法。 纳米团簇和微球可用于例如用于多重检测生物分子的测定中。

公开(公告)号：WO2005066373A1

公开(公告)日：2005-07-21

申请号：PCT/US2004/044091

申请日：2004-12-28

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei

IPC: C12Q1/68

CPC classification number: G01N21/658 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/543 ,  G01N33/68 ,  G01N33/6818 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  C12Q2565/125 ,  C12Q2565/632

Abstract: Various methods of using Raman-active or SERS-active probe constructs to detect analytes in biological samples, such as the protein-containing analytes in a body fluid are provided. The probe moieties in the Raman-active constructs are selected to bind to and identify specific known analytes in the biological sample or the probe moieties are designed to chemically interact with functional groups commonly found in certain amino acids so that the invention methods provide information about the amino acid composition of protein-containing analytes or fragments in the samples. In some cases, the Raman­active or SERS-active probe constructs, when used in the invention methods, can identify particular protein-containing analytes or types of such analytes so that a protein profile of a patient sample can be made. When compared to a data base of Raman or SERS spectra of normal samples, a disease state of a patient can be identified using the methods disclosed.

Abstract translation: 提供了使用拉曼活性或SERS-活性探针构建体检测生物样品中的分析物的各种方法，例如体液中含蛋白质的分析物。 选择拉曼活性构建体中的探针部分以结合并识别生物样品中的特定已知分析物，或者将探针部分设计为与通常在某些氨基酸中发现的官能团进行化学相互作用，使得本发明方法提供关于 样品中含蛋白质的分析物或片段的氨基酸组成。 在一些情况下，当在本发明方法中使用时，拉曼活性或SERS-活性探针构建体可以鉴定特定的含蛋白质的分析物或这种分析物的类型，使得可以制备患者样品的蛋白质谱。 当与正常样品的拉曼或SERS光谱的数据库相比时，可以使用所公开的方法鉴定患者的疾病状态。

公开(公告)号：WO2008085538A2

公开(公告)日：2008-07-17

申请号：PCT/US2007/072298

申请日：2007-06-27

Applicant: INTEL CORPORATION ,  ZHANG, Jingwu ,  LI, Handong ,  SUNDARARAJAN, Narayan ,  SU, Xing ,  SUN, Lei

Inventor： ZHANG, Jingwu ,  LI, Handong ,  SUNDARARAJAN, Narayan ,  SU, Xing ,  SUN, Lei

CPC classification number: G01N33/585 ,  C12Q1/6816 ,  G01N21/658 ,  G01N33/54373 ,  C12Q2563/155 ,  C12Q2565/632

Abstract: The embodiments of the invention are directed to a SERS cluster comprising a capture particle that is at least partially surrounded by analyte molecules, wherein both the capture particle and the analyte molecules surrounding the capture particle are at least partially surrounded by enhancer particles, wherein a majority of the analyte molecules are either sandwiched between capture and enhancer particles or located between junctions of the enhancer particles. The embodiments of the invention also relate to methods of manufacturing and detecting the SERS cluster. The embodiments of the invention also relate to a SERS active particle comprising a tag molecule comprising a Raman active compound and a probe or a linker having a specific biochemical binding capability and to a method for detecting of a target molecule using a SERS active particle having a tag molecule comprising a Raman active compound and a probe or a linker.

Abstract translation: 本发明的实施方案涉及包含被分析物分子至少部分包围的捕获颗粒的SERS簇，其中捕获颗粒和捕获颗粒周围的分析物分子至少部分地被增强子颗粒包围，其中多数 分析物分子被夹在捕获和增强剂颗粒之间或位于增强剂颗粒的结之间。 本发明的实施例还涉及制造和检测SERS簇的方法。 本发明的实施方案还涉及包含包含拉曼活性化合物的标签分子和具有特定生物化学结合能力的探针或接头的SERS活性颗粒以及使用具有下列化合物的SERS活性颗粒检测靶分子的方法： 标签分子，其包含拉曼活性化合物和探针或接头。

公开(公告)号：WO2006071946A2

公开(公告)日：2006-07-06

申请号：PCT/US2005/047278

申请日：2005-12-28

Applicant: INTEL CORPORATION ,  SU, Xing ,  SUN, Lei ,  GEORGER, Jacque

Inventor： SU, Xing ,  SUN, Lei ,  GEORGER, Jacque

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0819 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/5438 ,  C12Q2565/601

Abstract: Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte providing the reaction cavity between a source and a drain or a pair of electrodes, applying a voltage and monitoring a parameter indicative of an analyte characteristic. A process of fabricating an EACC include bonding an analyte to the multiple functional binding groups of each reaction cavity, and forming an analyte sensing structure including the substrate.

Abstract translation: 公开了用于生化分析物检测的电活性组合化学（EACC）芯片的装置和方法。 一种装置包括具有限定多个细胞的区域阵列的基底，其中每个细胞包括含有多个功能性结合基团的反应腔。 检测提供源和漏或一对电极之间的反应腔的分析物，施加电压并监测指示分析物特性的参数的方法。 制造EACC的方法包括将分析物结合到每个反应腔的多个功能结合基团，并形成包括基底的分析物检测结构。

公开(公告)号：WO2005066369A2

公开(公告)日：2005-07-21

申请号：PCT/US2004/043633

申请日：2004-12-28

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

IPC: C12Q1/68

CPC classification number: G01N21/658 ,  C12Q1/6869 ,  C12Q2565/632 ,  C12Q2533/101

Abstract: The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

Abstract translation: 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸掺入的顺序来确定。 提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。 此外，提供了用于检测单碱基重复的方法。

公开(公告)号：WO2005066367A2

公开(公告)日：2005-07-21

申请号：PCT/US2004/043364

申请日：2004-12-24

Applicant: INTEL CORPORATION ,  SU, Xing ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo ,  BERLIN, Andrew ,  SUN, Lei ,  ZHANG, Yuegang

Inventor： SU, Xing ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo ,  BERLIN, Andrew ,  SUN, Lei ,  ZHANG, Yuegang

IPC: C12Q1/68

CPC classification number: B82Y40/00 ,  B82Y10/00 ,  B82Y30/00 ,  C01B32/162 ,  C01B2202/08 ,  C01B2202/36 ,  D01F9/127 ,  H01L51/0048 ,  H01L51/0052

Abstract: The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles (140, 230) to polymer (120, 210) molecules, attaching the polymer (120, 210) molecules to a substrate, removing the polymer (120, 210) molecules and producing carbon nanotubes on the catalyst nanoparticles (140, 230). The polymer (120, 210) molecules alignment techniques. The nanotube arrays can be attached to selected areas (110, 310) of the substrate. Within the selected areas (110, 310), the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

Abstract translation: 本文公开的方法，装置和系统涉及碳纳米管的有序阵列。 在本发明的具体实施方案中，通过包括将催化剂纳米颗粒（140,230）附着到聚合物（120,210）分子上的方法形成纳米管阵列，将聚合物（120,210）分子连接到基底上，去除聚合物 120,210）分子并在催化剂纳米颗粒（140,230）上产生碳纳米管。 聚合物（120,210）分子对齐技术。 纳米管阵列可以附着到基板的选定区域（110,310）。 在所选择的区域（110,310）内，纳米管是非随机分布的。 本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列的装置以及包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统。 在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

公开(公告)号：WO2008005899A3

公开(公告)日：2008-01-10

申请号：PCT/US2007/072595

申请日：2007-06-29

Applicant: INTEL CORPORATION ,  SUN, Lei ,  KOO, Tae Woong ,  WANG, Liming

Inventor： SUN, Lei ,  KOO, Tae Woong ,  WANG, Liming

IPC: G01J3/44

Abstract: A device (and methods of using and manufacturing the device) that utilize a plurality of photomultipliers (PMT)s or a photodiodes coupled with a set of filters to collect Raman signal from samples. Also a method of detecting Raman signals includes receiving Raman signals from a sample utilizing a plurality of photomultiplier tubes (PMT)s or photodiodes, wherein at least one PMT or photodiode provides a different Raman signal than at least one other PMT or photodiode.